LOCUS       MR220472                8136 bp    DNA     circular  SYN 
DEFINITION  Ap3b2 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, beta 2 subunit (Ap3b2)
ACCESSION   NM_021492
VERSION     NM_021492.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4291..4382 
                     /label="Myc-DDK"  
     misc_feature    4412..4997 
                     /label="PolyA signal"  
     misc_feature    4469..4488 
                     /label="Restriction Enzyme"  
     misc_feature    5273..5945 
                     /label="ColE1"  
     misc_feature    6375..7169 
                     /label="Kan/Neo"  
     misc_feature    7204..7506 
                     /label="SV40 ori"  
     misc_feature    7592..8069 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4266..4273 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4266 
                     /label="Ap3b2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCGCT CCGGCCTACA GCGAAGACAA GGGCGGCTCA GCCGGTCCCG 
     1081 GGGAGCCAGA ATATGGCCAC GACCCGGCGA GCGGCGGTAT CTTCTCCTCG GACTACAAGC 
     1141 GGCATGATGA CCTGAAAGAG ATGTTGGACA CCAACAAGGA CTCCCTCAAG CTGGAGGCCA 
     1201 TGAAGAGGAT TGTGGCAATG ATCGCCCGGG GAAAGAATGC CTCGGACCTG TTCCCTGCAG 
     1261 TGGTGAAGAA TGTGGCCTGT AAGAACATAG AGGTGAAGAA GCTTGTCTAC GTGTATCTGG 
     1321 TCCGATATGC CGAGGAGCAG CAAGACCTCG CTCTACTGTC CATCTCTACT TTCCAACGTG 
     1381 GCCTAAAGGA TCCCAACCAG CTGATCCGTG CCAGTGCGCT CCGGGTTCTC TCTAGCATCC 
     1441 GTGTGCCCAT CATAGTGCCC ATCATGATGC TAGCCATCAA GGAAGCTGCC TCGGACATGT 
     1501 CACCCTACGT TCGGAAAACA GCTGCTCATG CCATCCCCAA ACTCTACAGT TTGGACTCCG 
     1561 ATCAGAAGGA CCAGCTGATA GAAGTCATTG AGAAGCTTTT GGCAGATAAG ACCACGCTGG 
     1621 TGGCAGGCAG TGTAGTGATG GCCTTTGAAG AGGTATGTCC AGAACGTATT GACCTGATTC 
     1681 ACAAAAACTA CCGGAAGCTC TGCAACCTGC TCATTGATGT GGAGGAGTGG GGCCAGGTGG 
     1741 TGATTATCAG CATGCTCACG CGTTATGCGC GAACGCAATT CCTGAGCCCT ACGCAGAATG 
     1801 AGTCCTTGCT GGAGGAGAAT CCCGAGAAGG CCTTCTATGG CTCCGAGGAG GACGAGGCCA 
     1861 AAGGCCCTGG ATCAGAGGAG GCAGCCACTG CGGCGCTGCC CGCTCGGAAG CCCTATGTAA 
     1921 TGGACCCAGA TCACCGGCTG CTGCTGCGGA ACACCAAGCC CCTGCTGCAG AGCCGCAGCG 
     1981 CCGCGGTGGT CATGGCGGTG GCGCAGCTTT ACTTCCACCT AGCGCCTAAG GCAGAGGTGG 
     2041 GCGTCATCGC CAAGGCGCTG GTACGCCTGC TGCGCAGCCA CAGTGAGGTG CAGTACGTAG 
     2101 TGCTCCAGAA CGTGGCCACC ATGTCCATCA AGCGCAGGGG TATGTTTGAA CCCTACCTGA 
     2161 AGAGCTTCTA CATTAGGTCC ACGGATCCTA CCCAGATCAA GATCCTGAAG CTGGAAGTGC 
     2221 TGACCAACCT GGCCAATGAG ACTAACATCC CCACTGTTCT CCGGGAATTC CAGACCTACA 
     2281 TTCGCAGCAT GGACAAAGAC TTTGTGGCAG CCACGATCCA GGCCATTGGA CGATGTGCAA 
     2341 CGAACATAGG CCGAGTCCGT GACACCTGTC TCAATGGCTT GGTGCAGCTT CTGTCCAACC 
     2401 GTGACGAGCT TGTGGTTGCA GAGTCAGTGG TGGTCATTAA GAAGCTGCTG CAAATGCAGC 
     2461 CAGCGCAGCA CGGTGAGATC ATCAAACACT TGGCAAAGCT CACAGACAAC ATCCAGGTGC 
     2521 CCATGGCACG AGCCAGCATC CTCTGGCTCA TTGGAGAGTA CTGTGAGCAT GTCCCCAAGA 
     2581 TCGCACCTGA TGTCCTGAGA AAAATGGCCA AGTCCTTCAC AGCAGAGGAG GACATCGTGA 
     2641 AGCTGCAGGT CATCAACCTG GCAGCCAAGC TGTACCTGAC TAACTCAAAG CAGACCAAGC 
     2701 TCTTGACCCA GTATGTGCTG AGTCTGGCCA AGTACGACCA GAACTATGAT ATCCGGGATC 
     2761 GAGCCCGCTT CACCCGGCAG CTCATTGTTC CCTCTGAGCA GGGTGGTGCC CTCAGTCGCC 
     2821 ATGCCAAGAA ACTCTTCCTG GCCCCTAAGC CAGCCCCTAT CTTGGAGTCA TCCTTCAAAG 
     2881 ATCGTGACCA TTTCCAGCTG GGCTCACTGT CACACCTGCT CAATGCAAAG GCTACAGGCT 
     2941 ACCAGGAGCT CCCGGACTGG CCAGAGGAAG CCCCAGATCC ATCTGTGCGC AATGTGGAGG 
     3001 TACCTGAATG GACCAAATGT TCAAATCGGG AGAAGAGGAA GGAGAAGGAA AAGCCTTTCT 
     3061 ATTCTGACTC CGAGGGGGAG TCTGGCCCCA CTGAGTCTGC AGACAGCGAG CCTGAATCTG 
     3121 AGAGTGAGTC AGAGAGTAAG AGCAGCAGTG GGAGTGGCTC CGGGGAGTCT AGCAGTGAGT 
     3181 CGGACAATGA AGAAGAGGAT GAGGAGAAAG GCGGAGGCAG CGAGAGTGAA CAGAGTGAGG 
     3241 AGGAAGATGA GAAGAAGAAG AAGACAAAGA AGAAGAAGGC ATCAGAGGGA CACAGAGAAG 
     3301 GCTCATCATC AGAAGAGGGC AGTGACTCCA GCAGCAGCTC AGAGTCTGAG GTGACGTCAG 
     3361 AGTCGGAGGA GGAACAGGTG GAACCTGCCT CCTGGAGGAA GAAAACGCCT CCAGGCAGCA 
     3421 AGAGTGCCCC TGTAGCCAAG GAGATTTCCC TGCTGGACCT AGAGGATTTC ACCCCTCCTA 
     3481 GTGTCCAGCC TGTGTCTCCT CCCATGGTTG TGTCTACCAG TCTGGCTGCT GACCTGGAAG 
     3541 GGCTGACACT CACAGACTCC TCTCTGGTGC CTTCGCTGCT GAGTCCAGTG TCCAGCATTG 
     3601 GGAGGCAGGA GCTGCTGCAC CGGGTAGCTG GAGAGGGGCT GTCTGTGGAC TATGCCTTCA 
     3661 GCCGCCAGCC TTTCTCTGGG GATCCCCACA TGGTGTCTCT GCATATCTAC TTCTCCAACA 
     3721 ACTCGGAGAC TCCCATCAAG GGGCTGCATG TGGGCACTCC CAAGCTGCCT GCTGGCATCA 
     3781 GCATTCAAGA ATTCCCTGAA ATCGAGTCCC TGGCACCTGG AGAATCCACC ACTACTGTGA 
     3841 TGGGCATTAA CTTCTGCGAC TCAACCCAGG CAGCCAACTT CCAGCTGTGC ACCCAAACCC 
     3901 GACAGTTCTA TGTCTCCATT CAGCCACCTG TCGGGGAGCT GATGGCCCCT GTATTTATGA 
     3961 GTGAGAATGA GTTCAAGAAG GAACAGGGAA AGCTGACAGG CATGAATGAG ATCACAGAGA 
     4021 AGCTTACATT GCCAGACACC TGTCGAAGTG ATCACATGGT GGTGCAGAAA GTGACTGCCA 
     4081 CCGCCAACCT AGGTCGTGTC CCATGTGGGA CATCTGATGA GTACAGGTTT GCAGGGAGGA 
     4141 CATTAACTAG TGGGAGTCTG GTCCTGCTGA CTCTGGATGC CCGGGCAGCT GGAGCCGCCC 
     4201 AGCTGACCGT CAACAGTGAG AAGATGGTGA TTGGCACCAT GCTGGTGAAG GATGTGATTC 
     4261 AGGCTCTGAC CCAGAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4321 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4381 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4441 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4501 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4561 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4621 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4681 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4741 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4801 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4861 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4921 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4981 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5041 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5101 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5161 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5221 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5281 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5341 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5401 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5461 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5521 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5581 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5641 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5701 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5761 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5821 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5881 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5941 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6001 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6061 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6121 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6181 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6241 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6301 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6361 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6421 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6481 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6541 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6601 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6661 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6721 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6781 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6841 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6901 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6961 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7021 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7081 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7141 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7201 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7261 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7321 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7381 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7561 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7621 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7681 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7741 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7801 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7861 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7921 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7981 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8041 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8101 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//