LOCUS       MR220414                7863 bp    DNA     circular  SYN 
DEFINITION  Clcn1 (Myc-DDK-tagged) - Mouse chloride channel 1 (Clcn1)
ACCESSION   NM_013491
VERSION     NM_013491.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4020..4111 
                     /label="Myc-DDK"  
     misc_feature    4141..4726 
                     /label="PolyA signal"  
     misc_feature    4198..4217 
                     /label="Restriction Enzyme"  
     misc_feature    5002..5674 
                     /label="ColE1"  
     misc_feature    6104..6898 
                     /label="Kan/Neo"  
     misc_feature    6933..7235 
                     /label="SV40 ori"  
     misc_feature    7321..7798 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4002..4008 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4002 
                     /label="Clcn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGGTCC CAGTCCCAGC GGCATGGAGG GGAACAGAGC TGGTGGGGCA 
     1081 GTGCCCCCCA GTACCAGTAC ATGCCCTTTG AACATTGTAC CAGCTACGGA CTGCCCTCAG 
     1141 AGAATGGGGG CCTTCAGCAC CGGCCCCGAA AGGACATGGG TCCCAGGCAC AATGCCCACC 
     1201 CAACACAGAT ATATGGCCAT CAAAAAGAAC AATATTCATA TAAGGCACAG GACGGGGGAA 
     1261 TGCCCAAGAA GATGGGCTCC AGTTCTACCA TGGACAGCTT GGATGAGGAC CACTATTCTA 
     1321 AATGTCAAGA CTGTGTCCAT CGCCTGGGAC GTGTGCTGAG AAGGAAGCTG GGGGAAGATT 
     1381 GGATCTTTCT TGTGCTCCTG GGCCTCCTGA TGGCTCTAGT CAGCTGGTGC ATGGACTATG 
     1441 TTAGTGCCAA GAGCCTTCAG GCCTACAAGT GGACCTATGC CCAGATGAAG CCTAGCCTTC 
     1501 CTCTGCAGTA CCTGGCCTGG GTCACCTTCC CACTTATCCT GATCCTCTTC AGCGCCCTCT 
     1561 TTTGCCAACT CATCTCTCCC CAGGCTGTGG GCTCTGGAAT CCCTGAGATG AAAACAATTC 
     1621 TTCGTGGTGT TGTCTTGAAG GAATACCTCA CACTCAAGGC CTTTGTAGCC AAGGTGGTAG 
     1681 CTTTGACAGC TGGACTGGGC AGTGGCATCC CTGTGGGGAA AGAGGGTCCC TTTGTTCACA 
     1741 TCGCCAGCAT CTGTGCTGCT GTCCTCAGCA AGTTTATGTC CATGTTCTCT GGTGTCTATG 
     1801 AGCAGCCATA CTATTACACT GACATCCTGA CAGTGGGCTG TGCCGTGGGG GTCGGTTGCT 
     1861 GTTTTGGAAC ACCACTTGGA GGAGTGCTAT TTAGCATCGA GGTCACCTCT ACCTACTTTG 
     1921 CTGTTCGGAA TTACTGGCGA GGATTCTTTG CAGCCACATT CAGTGCCTTT GTGTTCCGTG 
     1981 TCCTGGCCGT TTGGAACAAG GATGCTGTCA CCATCACTGC TCTGTTCAGA ACGAATTTCC 
     2041 GAATGGATTT CCCCTTTGAC CTGAAGGAAC TCCCAGCTTT TGCTGTCATT GGGATTTGCT 
     2101 GCGGGTTCTT GGGAGCTGTT TTCGTTTATC TGCATCGCCA AGTCATGCTC GGTGTCCGAA 
     2161 AGCACAAGTG TCTCAGCCAA TTTCTTGCTA AGCACCGCCT GCTATATCCT GGAATTGTTA 
     2221 CCTTTGTCAT CGCCTCGCTC ACATTTCCAC CAGGAATGGG TCAATTCATG GCTGGAGAGC 
     2281 TGATGCCCCG TGAAGCTATC AGTACCCTCT TTGACAACAA CACATGGGTA AAGCACATAG 
     2341 GTGACCCCCA AAGCTTGGGC CAGTCAGCTG TGTGGCTTCA CCCCCAGGTC AACGTGATCA 
     2401 TCATCATCCT TCTCTTCTTC GTTATGAAGT TTTGGATGTC CATTGTAGCC ACCACTATGC 
     2461 CCATACCCTG TGGAGGCTTC ATGCCTGTTT TTGTGCTAGG AGCTGCATTT GGAAGGCTGG 
     2521 TAGGAGAGAT CATGGCCATG CTATTCCCTG AGGGTATCTT ATTTGATGAT ATCATCTATA 
     2581 AGATCTTACC TGGGGGCTAT GCAGTAATTG GAGCAGCAGC TTTGACAGGG GCTGTCTCCC 
     2641 ACACAGTCTC CACAGCCGTC ATTTGCTTCG AATTAACCGG TCAGATTGCT CACATCCTGC 
     2701 CCATGATGGT GGCTGTTATC TTGGCCAACA TGGTGGCTCA GAGTCTGCAG CCCTCCCTCT 
     2761 ATGACAGCAT CATCCAGGTC AAGAAGCTTC CCTATCTGCC AGACCTTGGT TGGAACCAGC 
     2821 TCAGCAAATT TACAATTTTT GTTGAGGACA TCATGGTACG TGATGTGAAG TTCGTTTCAG 
     2881 CTTCTTGTAC ATATGGGGAA CTGAGAAACC TACTCCAGGC CACCACAGTC AAGACTTTAC 
     2941 CATTGGTTGA CTCCAAAGAT TCAATGATCC TGCTGGGCTC TGTGGAACGC TCAGAACTGC 
     3001 AGTCCCTCCT GCAGCGCCAC CTGTGTGCAG AGCGAAGGTT GAAGGCTGCC CAGGACATGG 
     3061 CTCGAAAGTT ATCAGAGCTG CCTTATAATG GCAAGGCTCA GCTGGCTGGG GACTGGCATC 
     3121 CTGGTGGTCG ACCTGAGTCC TTTGCCTTCG TAGATGAAGA TGAAGATGAG GACCTCTCCA 
     3181 GGAAGATGGA GCTTCCACTG ACTCCAGCTC CTCCCCCTCC TTCCCCTCCT CCTCCACCTT 
     3241 CCCAGTTTCC TATTGCTCCA TCAAACCCTG AAGAACCTAA TGGGCCACTG CCCAGCCATA 
     3301 AACAGCCACC TGAAGCCTCA GACTCTGCAG ATCAAAGATC ATCCACCTTC CAACGCCTGC 
     3361 TGCACTGCTT GCTAGGAAAA GCTCACTCCA AAAAGAAGAA AATAACACAG GATTCAACAG 
     3421 ATTTAGTGGA TAACATGTCA CCTGAAGAGA TTGAGGCCTG GGAGCGGGAG CAGCTGAGCC 
     3481 AGCCTGTGTG CTTTGATTGC TGCTGCATCG ACCAGTCTCC CTTCCAGCTG GTGGAGCAGA 
     3541 CAACCCTGCA CAAGACTCAT ACACTCTTCT CACTTCTTGG CCTCCATCTT GCCTATGTGA 
     3601 CCAGCATGGG GAAGCTCAGA GGTGTCTTGG CACTAGAAGA GCTACAGAAA GCTATTGAGG 
     3661 GCCACACCAA ATCTGGGGTG CAGCTTCGCC CTCCACTTGC CAGCTTCCGG AATACAACTT 
     3721 CAATCCGGAA GACTCCTGGG GGGCCACCCC CTCCTGCAGA GGGCTGGAAT GTACCTGAGG 
     3781 ATGGAGATGG GGCTCCTGGA AGAGAAGTCA TGGTTCCTAC CATGCCAGAG ACTCCTGTCC 
     3841 CACCACCATC TCCAGAGGCC CCTTCCTGCC TGGCCCCAGC CAGAGCGGAG GGTGAGCTGG 
     3901 AGGAACTGGA GATGGTGGGG AGCCTAGAGC CTGAGGAGGA GCTGGCTGAC ATCTTGCATG 
     3961 GCCCCAGTCT GCGGTCCACT GATGAGGAAG ATGAGGACGA GCTGATCCTG ACGCGTACGC 
     4021 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4081 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4141 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4201 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4261 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4321 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4381 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4441 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4501 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4561 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4621 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4681 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4741 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4801 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4861 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4921 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4981 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5041 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5101 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5161 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5221 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5281 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5341 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5401 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5461 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5521 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5581 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5641 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5701 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5761 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5821 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5881 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5941 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6001 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6061 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6121 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6181 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6241 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6301 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6361 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6421 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6481 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6541 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6601 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6661 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6721 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6781 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6841 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6901 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6961 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7021 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7081 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7141 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7261 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7321 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7381 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7441 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7501 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7561 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7621 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7681 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7741 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7801 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7861 TTT
//