LOCUS       MR220384                7041 bp    DNA     circular  SYN 
DEFINITION  Capn12 (Myc-DDK-tagged) - Mouse calpain 12 (Capn12)
ACCESSION   NM_001110807
VERSION     NM_001110807.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3198..3289 
                     /label="Myc-DDK"  
     misc_feature    3319..3904 
                     /label="PolyA signal"  
     misc_feature    3376..3395 
                     /label="Restriction Enzyme"  
     misc_feature    4180..4852 
                     /label="ColE1"  
     misc_feature    5282..6076 
                     /label="Kan/Neo"  
     misc_feature    6111..6413 
                     /label="SV40 ori"  
     misc_feature    6499..6976 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3180..3186 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3180 
                     /label="Capn12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGTGGC AACAGGAAGG TCACCATCCA GCTGGTGGAC GACGGGGCCG 
     1081 GGACTGGAGC TGGGGGCCCA CAGCTCTTTA AAGGCCAGAA CTACGAAGCC ATCCGAAGAG 
     1141 CTTGCCTGGA TTCCGGGATC CTGTTTCGTG ACCCTTGCTT TCCTGCTGGC CCTGATGCCC 
     1201 TTGGCTATGA CAAGCTGGGA CCTGACTCAG AGAAGGCCAA AGGGGTGGAA TGGAAGAGGC 
     1261 CCCATGAGTT TTGTGCTGAG CCCCAGTTCA TCTGTGAAGA CATGAGCAGA ACAGATGTGT 
     1321 GCCAGGGAAG CTTGGGAAAC TGCTGGCTTC TTGCAGCTGC TGCCTCCCTC ACACTCTACC 
     1381 CCAGGCTCCT GTACCGGGTG GTCCCCCCTG GACAAGGTTT CCAAGATGGC TACGCGGGGG 
     1441 TCTTCCATTT TCAGCTATGG CAGTTTGGCC GCTGGGTGGA TGTGGTGGTA GACGACAAAC 
     1501 TGCCTGTGCG TGAGGGGAAG CTGATGTTCG TGCGCTCAGA ACAAAGGAAC GAGTTCTGGG 
     1561 CCCCTCTGCT GGAAAAGGCC TATGCCAAGC TCCATGGCTC CTACGAGGTA ATGCGAGGAG 
     1621 GTCACATGAA CGAGGCTTTT GTGGACTTTA CAGGAGGCGT GGGTGAGGTC CTCTACTTGA 
     1681 GACAAAACAC TCCAGGTGTC TTTGCTGCCC TTCGCCACGC ATTGGCCAAG GAGTCCCTTG 
     1741 TGGGTGCTAC TGCCCTGAGT GATCGGGGTG AGATCCGCAC AGATGAAGGG CTGGTGAAGG 
     1801 GACATGCTTA TTCTGTCACA GGCACGCACA AGATGTCTCT GGGCTTCACC AAGGTGCGGC 
     1861 TGCTGCGGCT GAGGAACCCC TGGGGCCGCG TGGAGTGGTC CGGGCCCTGG AGTGACAGCT 
     1921 GCCCACGCTG GGACATGCTC CCTTCTGAGT GGCGAGATGC CCTGCTTGTG AAAAAGGAGG 
     1981 ATGGCGAGTT CTGGATGGAG CTTCAAGACT TTCTCACGCA CTTCAACACA GTGCAGATTT 
     2041 GTTCACTGAG TCCTGAGGTG TTGGGCCCCA GCCCTGCTGG CGGCGGCTGG CATATCCACA 
     2101 TCTTCCAGGG CCGCTGGGTG CGAGGCTTCA ACTCCGGTGG GAGTCAGCCC AGCGCTGAAA 
     2161 ACTTCTGGAC CAACCCCCAG TTCCGGCTGA CACTGCTGGA GCCTGATGAG GAAGAGGATG 
     2221 ACGATGATGA AGAGGGACCC TGGGGAGGCT GGGGAGCGGC AGGGGCCCGG GGCCCGGCGA 
     2281 GAGGAGGCCG AGTCCCCAAG TGCACGGTCC TGTTGTCACT CATCCAGCGC AACCGCCGGT 
     2341 GTCTGAGGGC CAAGGGCCTC ACTTACCTCA CTGTGGGCTT CCACGTGTTC CAGATTCCGG 
     2401 AGGAGCTGCT GGACCTCTGG GACTCCCCGC GCAGCCGCGC GCTCTTGCCG GGACTGCTGC 
     2461 GCGCCGACCG CTCGGTTTTC TGCGCCCGCC GCGACGTGAG CCGTCGCTGT CGCCTGCCGC 
     2521 CTGGCCACTA CCTGGTGGTA CCCAGCGCCT CGCGCGTAGG CGATGAAGCC GACTTCACTC 
     2581 TGCGCATCTT CTCGGAGCGC AGCCACACCG CAGTGGAGAT CGATGACGTG ATCAGCGCAG 
     2641 ACCTGGACGC CCTCCAGGCC CCCTACAAGC CCCTGGAGCT GGAGTTGGCA CAGCTATTTT 
     2701 TGGAGCTGGC TGGAGAGGAG GAGGAACTCA ACGCGCTTCA GCTGCAGACC TTAATAAGCA 
     2761 TTGCTCTGGA ACCTGCGAGG GCCAACACCA GGACCCCTGG AGAGATTGGG CTTAGGACCT 
     2821 GCGAACAGCT TGTGCAGTGT TTTGGGCGTG GGCAAAGACT GTCCCTACAC CACTTCCAGG 
     2881 AGCTCTGGGG CCATCTCATG TCATGGCAGG CCACATTTGA CAAGTTTGAT GAAGATGCCT 
     2941 CTGGGACAAT GAACTCCTGT GAACTGAGGC TGGCACTGAC TGCTGCAGGC TTCCACCTCA 
     3001 ACAACCAGCT GACCCAGTCC CTCACTAGCC GCTACCGGGA CAGCCGGCTC CGTGTGGACT 
     3061 TCGAGCGCTT CGTGTGCTGT GCAGCCCGGC TCACCTGCAT CTTCCGCCAC TGCTGCCAAC 
     3121 ACCTGGATGG CGGCGAGGGG GTCGTCTGCC TGACCCACAA ACAGTGGTCG GAGGTGGCTA 
     3181 CCTTCTCAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3241 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3301 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3361 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3421 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3481 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3541 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3601 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3661 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3721 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3781 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3841 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3901 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3961 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4021 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4081 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4141 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4201 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4261 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4321 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4381 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4441 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4501 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4561 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4621 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4681 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4741 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4801 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4861 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4921 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4981 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5041 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5101 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5161 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5221 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5281 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5341 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5401 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5461 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5521 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5581 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5641 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5701 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5761 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5821 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5881 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5941 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6001 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6061 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6121 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6181 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6241 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6301 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6361 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6421 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6481 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6541 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6601 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6661 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6721 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6781 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6841 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6901 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6961 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7021 CAAAAATTTA ACGCGAATTT T
//