LOCUS       MR220320                8610 bp    DNA     circular  SYN 
DEFINITION  Pitpnm1 (Myc-DDK-tagged) - Mouse phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant 2
ACCESSION   NM_001136078
VERSION     NM_001136078.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4767..4858 
                     /label="Myc-DDK"  
     misc_feature    4888..5473 
                     /label="PolyA signal"  
     misc_feature    4945..4964 
                     /label="Restriction Enzyme"  
     misc_feature    5749..6421 
                     /label="ColE1"  
     misc_feature    6851..7645 
                     /label="Kan/Neo"  
     misc_feature    7680..7982 
                     /label="SV40 ori"  
     misc_feature    8068..8545 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4749..4755 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4749 
                     /label="Pitpnm1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTATCAAG GAGTACCACA TCCTGCTGCC CATGAGCCTG GACGAGTATC 
     1081 AAGTGGCCCA GCTCTACATG ATCCAGAAAA AGAGCCGTGA GGAGTCTAGT GGTGAGGGCA 
     1141 GCGGCGTGGA GATCCTGGCC AACCGGCCCT ACACAGATGG GCCTGGAGGC AACGGGCAGT 
     1201 ATACACACAA GGTGTACCAC GTGGGCTCCC ACATCCCAGG CTGGTTCCGG GCACTGTTAC 
     1261 CCAAGGCTGC TCTGCAGGTA GAAGAGGAAT CTTGGAACGC TTACCCATAT ACCCGGACAC 
     1321 GGTACACCTG CCCCTTTGTG GAGAAGTTCT CCATTGAGAT AGAGACCTAC TACTTGCCTG 
     1381 ATGGGGGGCA GCAACCTAAC GTCTTCAACC TGAGTGGGGC TGAGAGAAGA CAGAGAATCG 
     1441 TGGATACCAT CGACATCGTG CGGGATGCAG TGGCCCCAGG AGAATACAAA GCGGAAGAGG 
     1501 ACCCTCGGCT GTACCGCTCA GCCAAGACAG GCCGAGGGCC GCTGGCTGAT GACTGGGCAC 
     1561 GGACAGCGGC CCAGACAGGA CCTCTCATGT GTGCCTATAA GCTGTGCAAG GTTGAATTCC 
     1621 GCTACTGGGG CATGCAGGCC AAGATTGAGC AGTTCATCCA TGACGTAGGT CTGCGCAGGG 
     1681 TGATGCTTCG CGCCCATCGC CAGGCCTGGT GTTGGCAAGA TGAGTGGATA GAACTGAGCA 
     1741 TGGCTGACAT CCGGGCACTG GAGGAGGAGA CTGCACGCAT GCTAGCGCAG CGTATGGCTA 
     1801 AGTGCAACAC TGGCAGTGAG GGACCAGAGG CTCAGACCCC TGGGAAATCC AGCACTGAGG 
     1861 CCCGACCTGG GACCAGCACT GCTGGCACCC CTGATGGACC TGAGGCCCCT CCCGGCCCCG 
     1921 ACGCCTCCCC AGATGCCAGC TTTGGGAAGC AGTGGTCCTC ATCCTCCCGT TCCTCCTACT 
     1981 CATCCCAACA TGGAGGCGGC GTGTCTCCAC AGAGCTTGTC TGAGTGGCGC ATGCAGAACA 
     2041 TTGCCCGAGA CTCTGAGAAC AGCTCCGAGG AAGAATTCTT TGATGCCCAT GAAGGTTTCT 
     2101 CGGACAGTGA TGAGGTCTTC CCCAAGGAGA TGACCAAGTG GAATTCCAAT GATTTTATCG 
     2161 ATGCCTTTGC CTCCCCAACC GAGGTGGAGG GGGTACCAGA TCCTACAGTC ATGGCTACCA 
     2221 AAGGCATTGA AGATGGGGCC CGAGCTCCCA GGGACTCAGA GGGCCTAGAT GGAGCGGGGG 
     2281 ATCTAGTGGT CGAGGCGTGC TCTGTGCATG CCCTCTTCCT CATCCTGCAC AGCGGCAGCA 
     2341 TCCTGGACTC TGGCCCTGGA GACACCAACT CCAAGCAGGC CGACGTGCAA ACGCTGAGCA 
     2401 CAGCCTTTGA GGCCGTCACC CGAGTCCATT TCCCCGAGGC CCTGGGTCAC GTGGCACTGC 
     2461 GGCTGGTGCC CTGTCCACCC ATTTGCGCGG CTGCCTATGC TCTTGTCTCC AACCTGAGCC 
     2521 CCTACAGCCA TGATGGGGAT AGCCTGTCCC GCTCCCAAGA CCACATTCCG CTGGCTGCCC 
     2581 TGCCGCTCCT GGCCACCTCA TCCTCTCGCT ACCAGGGTGC CGTGGCCACT GTCATCGCTC 
     2641 GCACCAACCA GGCCTATGCA GCCTTCCTGC GCTCATCGGA GGGCACAGGT TTCTGCGGGC 
     2701 AGGTGGTGCT GATCGGAGAT GGTGTTGGTG GCATCCTGGG CTTTGATGCG CTCTGCCACA 
     2761 GTGCCAGTGC AGGCCCAGGG AGTCGGGGCA GCAGCCGCCG TGGGAGCATG AACAATGAGA 
     2821 TGCTCTCCCC TGAAGTTGGT CCAGTACGGG ACCCGCTGGC AGATGGGGTG GAGGTACTGG 
     2881 GTCGTGCTAG CCCAGAACCC TCAGCCCTAC CTGCTCAGCG TACCTTCAGT GACATGGCCA 
     2941 ATCCTGATCC TGATGGCTCT CAGAACAGCC TCCAGGTAGC CTCCACAGCT ACCTCCTCTG 
     3001 GGGAGCCCCG CCGTGCAAGC ACAGCCTCCT GTCCACCTGC CAGTTCTGAG GCTCCTGACG 
     3061 GCCCCACGAA CGCTGCTCGC CTGGACTTCA AGGTCTCAGG CTTCTTCCTC TTTGGCTCCC 
     3121 CACTGGGCCT GGTGCTGGCT CTTCGCAAAA CCGTGATGCC CGCCTTGGAG GTGGCTCAGC 
     3181 TGCGTCCAGC CTGCGAGCAG ATCTACAATC TCTTCCATGC CGCTGACCCC TGTGCCTCCC 
     3241 GCCTGGAACC CCTGCTGGCC CCCAAGTTCC AGGCCATCGC CCCCCTGGCT GTACCTCGTT 
     3301 ACCAGAAGTT TCCCCTAGGA GACGGCTCGT CCTTGCTGCT GGCTGACACT TTGCAAACCC 
     3361 ATTCGAGTCT CTTCCTGGAA GAGCTGGAGA TGATGGTGCC CTCCACACCC ACCTCGGCCA 
     3421 GTGGTGCCTT CTGGAAGGGC AGTGAACTAG GCAATGAGCC AGCATCCCAG ACAGCAGCCC 
     3481 CCAGCACCAC CAGCGAGGTT GTTAAGATCC TGGACCGCTG GTGGGGAAAC AAGCGGATCG 
     3541 ACTATTCACT GTACTGCCCC GAGGCACTCA CGGCCTTCCC CACGGTCACG CTGCCCCACC 
     3601 TCTTCCACGC CAGCTACTGG GAATCAGCGG ATGTAGTGGC CTTCATTCTG CGCCAGGTCA 
     3661 TTGAGAAGGA GCGGCCACAG CTGACAGAGT GTGAGGAGCC ATCCATCTAT AGCCCCGCCT 
     3721 TCCCCAGGGA GAAGTGGCAG CGCAAACGCA CACAGGTCAA GATCCGGAAC GTCACTTCCA 
     3781 ACCACCGAGC GAGTGACACC GTGGTATGTG AGGGCCGTCC CCAGGTGCTG AATGGGCGCT 
     3841 TCATGTATGG ACCATTGGAT GTGGTCACGC TCACTGGAGA GAAGGTGGAT GTCTACGTCA 
     3901 TGACACAGCC ACTGTCAGGC AAGTGGATCC ACTTTGGCAC AGAGGTCACT AACAGCTCAG 
     3961 GCCGTCTCAC CTTCCCAGTG CCCTCAGAAC GTGCACTGGG CATTGGTGTC TACCCTGTGC 
     4021 GCATGGTGGT CAGGGGAGAC CACACCTATG CCGAGTGCTG TCTGACTGTG GTGTCCCGAG 
     4081 GCACAGAAGC TGTGGTCTTC AGCATCGATG GTTCTTTCAC TGCCAGTGTC TCTATCATGG 
     4141 GCAGCGACCC CAAGGTGCGC GCTGGCGCAG TGGACGTGGT CAGGCACTGG CAGGACTCTG 
     4201 GCTACTTGAT TGTGTATGTA ACTGGCCGGC CTGACATGCA AAAGCACCGT GTAGTGGCCT 
     4261 GGCTGTCGCA ACACAACTTC CCCCATGGTG TTGTCTCCTT CTGTGATGGC CTCACCCACG 
     4321 ACCCGCTGCG GCAGAAAGCC ATGTTTCTGC AGAGCCTGGT GCAAGAGGTA GAACTGAACA 
     4381 TCGTGGCTGG ATATGGGTCA CCAAAAGATG TGGCAGTGTA TGCAGCACTG GGGCTCTCTC 
     4441 CGAGCCAGAC CTACATTGTT GGCCGTGCTG TGCGCAAACT GCAGGCACAG TGTCAGTTCC 
     4501 TGTCAGATGG CTATGTGGCC CACTTGGGCC AGCTGGAGGC AGGCTCCCAC TCCCATGCTC 
     4561 CCTCAGGACC TCCGAGAGCC GCTCTGGCCA AGAGTAGCTA TGCTGTGGCT GCCCCTGTGG 
     4621 ACTTCCTCCG GAAGCAGAGC CAGCTGCTTC GATCCAGAGG CCCTAGCCAG GTGGACCGTG 
     4681 AGGGTCCAGG AACACCTCCC ACCACCCTGG CCAGGGGCAA GACTCGCAGC ATCAGCCTCA 
     4741 AGTTGGACAG TGAAGAGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4801 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4861 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4921 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4981 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5041 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5101 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5161 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5221 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5281 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5341 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5401 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5461 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5521 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5581 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5641 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5701 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5761 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5821 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5881 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5941 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6001 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6061 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6121 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6181 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6241 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6301 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6361 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6421 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6481 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6541 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6601 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6661 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6721 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6781 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6841 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6901 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6961 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7021 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7081 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7141 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7201 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7261 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7321 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7381 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7441 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7501 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7561 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7621 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7681 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7741 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7801 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7861 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7981 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8041 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8101 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8161 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8221 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8281 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8341 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8401 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8461 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8521 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8581 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//