LOCUS       MR220302                6798 bp    DNA     circular  SYN 
DEFINITION  Ccdc27 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 27 (Ccdc27)
ACCESSION   NM_001033455
VERSION     NM_001033455.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2955..3046 
                     /label="Myc-DDK"  
     misc_feature    3076..3661 
                     /label="PolyA signal"  
     misc_feature    3133..3152 
                     /label="Restriction Enzyme"  
     misc_feature    3937..4609 
                     /label="ColE1"  
     misc_feature    5039..5833 
                     /label="Kan/Neo"  
     misc_feature    5868..6170 
                     /label="SV40 ori"  
     misc_feature    6256..6733 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2937..2943 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2937 
                     /label="Ccdc27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCAGGAG AACTTGACTC AGGAATGCTG CTCCACATCC CCCAAGATCA 
     1081 GCAAGGGCCT GATGGTGCTT CAGAGCATAG CCAGCCGGGG CTGCGACACA CAGGACTCAG 
     1141 AGAAGAAACC GCAAAGTACC CAGCAAAGCC TCAAAAAGTC AAGCCAGGCG GCTGCCGGAT 
     1201 ACTTCTATGA CAAGGAGGAC CAGATCAGGA AACTCAGCAA ACACAACGGC TTTTTGTCTG 
     1261 AAATGGAAGA TATGAGAAAA GCCTTCCTCA TGCGGCCTGG GTGTCCCCAG TTTAGTACCA 
     1321 GGACAACATC CATGTCTCAT GTGGGTTCTG CCATTATGGT TGATCTTCCA AGGACCTGCT 
     1381 CTGGAGTTTG GAAGTTGACT GAGGACCATC CCCTAGGCAG GCTGGGCTCT GCCAGCAGTG 
     1441 TGGATGGGAG GGTCTTTCCA TTCAGCAAGA GTGCTTGTGA GCTCAACTAC CCACGGAAGA 
     1501 GGAGTGAGCC TTCAGACCCG AGTCCCACCG GCAGCCCCAC TGTGGTCAAG AAGTCCCAGA 
     1561 GGACCAGAAC GCCCTGGTAT ATCTCAGTCA TCCATGAGAA GGATCACTCT CTGCTCCTGA 
     1621 TGGGAGAAGA GCTCCAGCGT TTCTCCGAGA TGGAGAGCCA GATGCAGAAG AAAGATCAGG 
     1681 AGATCCTGAC ACTCCAGAAG GAGAAGGAGG CCCTGAAGAA GCAGCTGAAG AACCTGCTCA 
     1741 GAGGCAAAGG CACAGAAACC TCTTCAGCTT CCATCAAGAT GGACCGGTCC TTTGAGACGC 
     1801 CCCTGAAGCT GGGGAGGATG AGTGTGCTGA AGACCATCTA CAAAGAAGAG GATGAGCTAC 
     1861 AGCACTGGAT GCAGATGCAG GAGGAATATA GCATGGCGGA GAGTAGCAAG GAGCTGCACG 
     1921 TGGAACCAGG AAGCGCCATA GAGGAAAAGA GCTCTGAGGG GCCACCCGAG GAGGCTGCAG 
     1981 CTGCCAAACT GAGCAGGCCC AGCCAGAGCA AGACTGAGAC GCTGCTGGAG GTGGGCCCCG 
     2041 AGGAGGAGGA GGAGGAGGAA GAGGAGGAGG TGGAGGGTGA TGAAGCAAAA GGAACTGAGG 
     2101 AGGGAGAGAT ATTGGTGAAT GAGGAAGAGG CATCGTGGGA GCTTCGGGAG GACGAGGAAT 
     2161 GTCACCCCAA GAGGTCCTAC TCCATGACGG AGTCCTTTGA GGAGGAGCTG ATGGCTCAGC 
     2221 TGGAGGAGTA TGAACGGATG TTGATGGACT TCCAGAGAGA GCTGGAATTC ACTAGGTCCA 
     2281 GATATTCCCT GGCCACAGGG ACCATCACAT CTTTGCAGCG GCAAACTGAC TTCCAAGAGT 
     2341 CTCAGCTGCG GAAGGTCACC ACGGAGAACG AGCTGCTGGA GAAGGAGCTC CGGGAACGGA 
     2401 AGCAGCAGAT ACAAGACATG ACAGACAAGT TCTCCAACCT CCGGGAGGAA AAGAAGCATC 
     2461 AGGAGATAAT GGGACTCATT GAGAAGGAAA ACCTGGTCCT CCGACAGCAA GTTGCAGACT 
     2521 TGAAGATGGA TCTCATCAGC AGTGAGCGGA CTATTAAAGA GCTGAACACC CAAACCAAAG 
     2581 AGCTGGAAGA TCAGGTTAAC ACCGACAAGG ACCACCTGAG GCGGTGGAAG GATTTGCATG 
     2641 ACGACCTACA GACACGTAAC GAGATCATCC AACAGACAGA GCAACAGACT CGTGTGGTCC 
     2701 TGGAGGCCAC CCAGGCCAGG TATGAGAAGC TAAGGAATAA GATCATCCAG GCTGTCTTCA 
     2761 GTGTCAGTGG GAACAAGAAC TTGTCCATGG AACTCTCTGA TAGCTACATC CTGGAATCCC 
     2821 TGCAGAGGAT CATCTCGGAG AGGAGTGACT TCTACAGTCA GCTGAAGCAA AAGGGCGTGA 
     2881 AGGTGCCCCC ACTACAGCAG TCAGATGTCT CCCTGCCTAG CAAGATCAAG AAGATGGCAT 
     2941 CCAAGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3001 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3061 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3121 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3181 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3241 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3301 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3361 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3421 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3481 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3541 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3601 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3661 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3721 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3781 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     3841 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     3901 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     3961 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4021 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4081 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4141 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4201 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4261 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4321 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4381 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4441 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4501 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4561 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4621 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4681 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4741 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4801 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     4861 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     4921 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     4981 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5041 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5101 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5161 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5221 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5281 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5341 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5401 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5461 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5521 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5581 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5641 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5701 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5761 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     5821 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     5881 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     5941 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6001 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6061 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6121 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6181 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6241 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6301 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6361 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6421 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6481 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6541 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6601 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6661 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6721 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6781 AAATTTAACG CGAATTTT
//