LOCUS       MR220287                7983 bp    DNA     circular  SYN 
DEFINITION  B4galnt4 (Myc-DDK-tagged) - Mouse beta-1,4-N-acetyl-galactosaminyl transferase 4 (B4galnt4)
ACCESSION   NM_177897
VERSION     NM_177897.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4140..4231 
                     /label="Myc-DDK"  
     misc_feature    4261..4846 
                     /label="PolyA signal"  
     misc_feature    4318..4337 
                     /label="Restriction Enzyme"  
     misc_feature    5122..5794 
                     /label="ColE1"  
     misc_feature    6224..7018 
                     /label="Kan/Neo"  
     misc_feature    7053..7355 
                     /label="SV40 ori"  
     misc_feature    7441..7918 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4122..4128 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4122 
                     /label="B4galnt4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGTGGTTC CCGGTGAAGA AGGTCCGCAA GCAGATGAAG CTGCTGCTGC 
     1081 TGTTGCTGCT GCTCACCTGC GCCGCGTGGC TCACGTATGT GCACCGGAGC CTGGTGCGCC 
     1141 CGGGCCGCGC GCTACGCCAG CGGCTGGGCT ACGGGCGAGA TGGGGAGAAG CTGACCGGTG 
     1201 TGACCGATAG CCGCGGAGTC CGAGTGCCAT CGTCCACACA GAGGTCGGAG GACTCGAGTG 
     1261 AAAGTCATGA AGAGGAGCAG GCGCCCGAGG GGCGGGGCCC AAACATGCTG TTTCCTGGAG 
     1321 GACCTAGGAA GCCACCCCCA CTGAACCTCA CCCACCAGAC ACCCCCATGG CGGGAAGAGT 
     1381 TCAAAGGACA GGTGAACCTG CACGTGTTTG AGGACTGGTG TGGAGGTGCT GTGGGCCACC 
     1441 TGAGACGGAA TCTGCACTTC CCACTCTTTC CTCACACTCG TACTACGGTG ACAAAGTTAG 
     1501 CTGTGTCCCC TAAGTGGAAG AACTATGGAC TCCGGATTTT TGGCTTCATC CACCCAGCCA 
     1561 GAGATGGAGA CATCCAGTTC TCTGTGGCTT CGGATGACAA CTCTGAGTTC TGGCTGAGTT 
     1621 TGGATGAGAG CCCAGCAGCC GCCCAGCTTG TAGCCTTTGT GGGCAAGACT GGCTCCGAGT 
     1681 GGACCGCACC TGGAGAATTC ACCAAGTTCA GCTCCCAGGT GTCTAAGCCA CGTCGGCTCA 
     1741 TGGCCTCCCG GAGATACTAC TTTGAACTGC TCCACAAGCA AGATGACAAG GGTTCAGACC 
     1801 ATGTGGAAGT GGGTTGGCGA GCTTTCCTGC CTGGTCTGAA GTTCGAGATC ATTGATTCTG 
     1861 CTCACATTTC CCTGTACACA GATGAGTCAT CTCTGAAGAT GGACCATGTG GCCCATGTGC 
     1921 CTCAGTCTCC AGCCAGCCAC ATAGGAGGAT TCCCGCCGCA GGGGGAACCC AGCGCCGACA 
     1981 TGCTGCACCC AGACCCCAGG GATACCTTCT TCCTCACTCC TCGGATGGAA CCTTTGAGCC 
     2041 TGGAGAATGT TCTGGAGCCC TGTGCCTATG CCCCCACCTA TATCCTCAAG GATTTCCCCA 
     2101 TAGCCAGATA CCAAGGACTA CAGTTTGTGT ACCTGTCCTT CATCTACCCC AATGACCATA 
     2161 CCCGTCTCAC TCACATGGAG ACAGACAACA AGTGCTTCTA CCGTGAGTCC CCACTATACC 
     2221 TGGAAAGGTT TGGGTTCTAT AAATACATGA AAATGGACAA GGAGGAGGGA GAGGAAGATG 
     2281 AGGAGGAAGA AGTTCAGCGT AGAGCCTTCC TCTTCCTCAA CCCAGATGAC TTCCTGGATG 
     2341 AGGAGGATGA GCAGGATCTG TTAGACAGCC TGGAGCCCAC CGATGCATCT GTACAGCAGA 
     2401 GCCACAGGAC CCCCACCCCA GCAGCCTCCA CTGGAACGAC AGCCAGCCCG ACCCCACCTA 
     2461 CAACTAGTCC TCTGGACGAG CAGACCCTCA GACACTCCCG GGCACTGAAT TGGGCCCCAC 
     2521 GCCCCCTGCC CCTCTTCTTG GGGCGAGCTC CACCTCCCCG AACTGTGGAG AAGTCGCCTT 
     2581 CAAAGGTGTA CGTGACCAGG GTCCGACCTG GACAGCGGGC TTCCCCGAGG GCATTGCGAG 
     2641 ACTCACCCTG GCCACCCTTC CCTGGCGTCT TCCTGCGCCC CAAGCCTCTG CCCAGAGTAC 
     2701 AGCTGCGGGT ACCCCCACAT CCACCTCGGA CCCAGGGCTA TAGGACCAGT GGCCCCAAGG 
     2761 TCACAGAACT AAAGCCCCCA GTCAGGGCCC AGACCAGCCA GGGAGGCCGG GAGGGCCAGT 
     2821 TACATGGACA GGGACTCATG GTGCCCACAG TGGACTTGAA CTCCTCAGTG GAAACACAGC 
     2881 CTGTGACTTC CTTCCTGAGC TTGTCTCAGG TATCCAGGCC ACAGCTGCCA GGAGAGGGTG 
     2941 AAGAAGGGGA GGAGGATGGG GCCCCAGGTG ATGAGGCCAC ATCAGAAGAC AGTGAGGAAG 
     3001 AGGAGGAGCC GGCCGCTGGG CGGCCCCTGG GTCGCTGGCG GGAGGATGCC ATCAACTGGC 
     3061 AGCGCACGTT CAGCGTGGGC GCCATGGACT TCGAGCTCCT GCGCTCTGAC TGGAACGACC 
     3121 TGCGCTGTAA CGTATCCGGG AACCTGCAAC TTCCTGAGGC CGAAGCGGTG GATGTAGTGG 
     3181 CTCAGTACAT GGAGCGGCTA AATGCAAAGC ATGGCGGGCG CTTCTCGCTT CTACGCATCG 
     3241 TGAACGTGGA GAAGCGCCGC GACTCTGCAC GCGGGAGCCG CTTCCTCCTG GAACTGGAAT 
     3301 TGCAAGAGCG CGGAGGGAGC CGCCAGCGCC TATCCGAATA CGTCTTCCTG CGGTTGCCCG 
     3361 GAGCCCGCGT TGGGGACGAA GATGGAGAAA GTCCCGAGCC GCCTCCAGCC GCCTCGATCC 
     3421 ACCCAGACAG TCGCCCAGAG CTCTGCCGGC CTTTGCATCT GGCCTGGCGT CAGGATGTCA 
     3481 TGGTTCATTT CATTGTACCA GTGAAGAATC AGGCGCGCTG GGTAGTGCAG TTCCTGGCAG 
     3541 ATATGACCGC GCTGCATGTG CATACGGGGG ACTCGTACTT CAACATCATC TTGGTGGACT 
     3601 TTGAGAGCGA GGACATGGAT GTGGAGCGGG CCCTGCGTGC GGCTCAGCTA CCTCGGTACC 
     3661 AGTACTTGAA ACGAACTGGA AACTTCGAGC GCTCTGCAGG CCTGCAAACT GGAGTGGATG 
     3721 CCGTGGAGGA CCCCAGCAGC ATCGTTTTCC TCTGTGACCT GCACATCCAC TTCCCACCTA 
     3781 ATATCCTGGA CAGCATCCGC AAGCATTGCG TGGAGGGCAA GCTGGCCTTC GCCCCTGTGG 
     3841 TCATGCGTCT GGGCTGTGGA AGCTCACCGT GGGACCCACA TGGTTACTGG GAAGTGAATG 
     3901 GATTTGGCCT CTTTGGGATC TACAAATCAG ACTTTGACAG AGTAGGAGGC ATGAACACTG 
     3961 AGGAGTTCCG TGACCAGTGG GGAGGCGAGG ACTGGGAACT TCTTGACAGG GTCCTGCAGG 
     4021 CAGGGCTGGA GGTGGAGAGG CTTCGACTGC GACACTTCTA CCACCACTAT CACTCGAAGC 
     4081 GAGGCATGTG GGCCACACGC AGCCGCAAAG GTGCCCGCGC ACAGCGATCC ACGCGTACGC 
     4141 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4201 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4261 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4321 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4381 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4441 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4501 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4561 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4621 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4681 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4741 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4801 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4861 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4921 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4981 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5041 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5101 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5161 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5221 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5281 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5341 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5401 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5461 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5521 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5581 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5641 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5701 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5761 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5821 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5881 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5941 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6001 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6061 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6121 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6181 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6241 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6301 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6361 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6421 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6481 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6541 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6601 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6661 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6721 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6781 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6841 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6901 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6961 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7021 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7081 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7141 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7201 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7261 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7321 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7381 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7441 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7501 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7561 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7621 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7681 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7741 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7801 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7861 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7921 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7981 TTT
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