LOCUS       MR220267                8205 bp    DNA     circular  SYN 
DEFINITION  Gucy2f (Myc-DDK-tagged) - Mouse guanylate cyclase 2f (Gucy2f)
ACCESSION   NM_001007576
VERSION     NM_001007576.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4362..4453 
                     /label="Myc-DDK"  
     misc_feature    4483..5068 
                     /label="PolyA signal"  
     misc_feature    4540..4559 
                     /label="Restriction Enzyme"  
     misc_feature    5344..6016 
                     /label="ColE1"  
     misc_feature    6446..7240 
                     /label="Kan/Neo"  
     misc_feature    7275..7577 
                     /label="SV40 ori"  
     misc_feature    7663..8140 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4344..4350 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4344 
                     /label="Gucy2f"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCCTCGGA CCCTGGCCTT TTTCTCGCCT TCTGTCCTGG TTTGCCATTT 
     1081 CCAGCAGACT GTCAGGACAG CATGGCCTCC CTTCCTCCAA GTTCCTGAGA TGTTTGTGCC 
     1141 TCTTGGCCCT TTTGCCTCTC CTTCGGTGGG GGCAAGCACT CCCCTACAAG ATCGGGGTCA 
     1201 TAGGTCCCTG GACATGTGAT CCGTTCTTTT CCAAGGCCCT GCCTGAGGTT GCTGCTGCTT 
     1261 TAGCAATTGA GCGAATCAGC CGGGACAAGA CATTTGACAG GAGTTACTCC TTCGAATACG 
     1321 TGATTCTTAA CGAAGACTGT CAGACTTCAA AGGCCCTCGC CAGTTTCATT TCCCACCAGC 
     1381 AGATGGCCTC AGGATTTGTT GGGCCTGCCA ACCCTGGCTT CTGCGAGGCA GCCTCACTCC 
     1441 TGGGAACCAG CTGGGACAAA GGGATTTTCT CTTGGGCTTG TGTGAATCAT GAATTAGATA 
     1501 ACAAACATAG CTTCCCAACC TTCTCTCGGA CACTCCCTTC TCCGATACGG GTGCTTGTAA 
     1561 CCGTCATGAA GTATTTCCAG TGGGCACATG CTGGGGTCAT TTCCTCAGAT GAAGACATTT 
     1621 GGATGCATAC AGCCAATCGA GTCTCAAGTG CTCTTCGGAG CCAGGGCCTA CCTGTAGGGG 
     1681 TCGTCCTGAC CTCGGGACGA GACAGCCAGA GCATTCAGAA AGCTCTCCAG CAGATTCGCC 
     1741 AGGCAGACAG AATTCGCATA ATCATAATGT GTATGCATTC AGCCTTGATT GGGGGAGAGA 
     1801 CACAAACACA CTTCTTGGAA TTGGCTCATG ATCTGAAAAT GACAGATGGG ACTTACGTCT 
     1861 TTGTCCCCTA TGATGTACTG CTCTACAGTT TACCTTATAA GCACAGCCCC TACCAGGTCC 
     1921 TGAGGAACAA TCCAAAGCTC CGGGAAGCCT ATGATGCTGT GTTGACTATT ACAGTGGAGT 
     1981 CCCATGAAAA GACCTTCTAC GAAGCCTATG CAGAAGCAGC AGCCCGCGGA GAAATTCCTG 
     2041 AGAAGCCTGA CTCAAACCAA GTTTCACCGT TGTTTGGAAC CATCTACAAT TCAATTTATT 
     2101 TTATCGCACA AGCCATGAAT AATGCTATGA AAAAAAATGG ACGGGCTAGT GCTGCCAGCC 
     2161 TGGTTCAGCA TTCCAGAAAC ATGCAGTTCT ATGGATTCAA CCAGTTGATA AAGACAGACT 
     2221 CAAATGGAAA TGGGATTTCT GAATATGTAA TCCTGGACAC CAATGGGAAA GAATGGGAAC 
     2281 TTCGTGGCAC CTACACTGTG GACATGGAAA CTGAACTGCT AAGGTTCAGA GGGACCCCAA 
     2341 TTCATTTCCC TGGCGGCAGA CCTACTAGTG CAGATGCAAA ATGCTGGTTT GCAGAAAGGA 
     2401 AGATCTGCCA AGGAGGTATT GACCCTGCCT TGGCCATGAT GGTCTGCTTT GCTTTACTTA 
     2461 TAGCCTTGCT GTCTATCAAT GGATTTGCTT ACTTTATAAG GCGACGTATA AATAAAATCC 
     2521 AGTTGATTAA AGGACCCAAC AGAATCCTAC TGACTTTGGA AGATGTGACA TTTATTAATC 
     2581 CCCACTTTGG CAGTAAGAGA GGAAGCCGTG CCAGTGTAAG CTTCCAGATC ATCTCAGAAG 
     2641 TTCAAAGTGG GAGGTCCCCA AGGCTGTCCT TTTCTTCAGG TAGTCTAACT CCAGCTACAT 
     2701 ATGAGAACTC CAACATAGCA ATCTATGAGG GTGACTGGGT ATGGCTGAAA AAGTTCCCTC 
     2761 CTGGAGATTT TGGAGACATT AAGTCTATCA AATCGAGTGC AAGTGATGTG TTTGAAATGA 
     2821 TGAAGGACCT GCGGCATGAA AATGTCAACC CTCTATTGGG TTTCTTCTAT GATTCGGGGA 
     2881 TGTTTGCCAT CGTGTCAGAA TTTTGTTCTC GAAGGAGCCT AGAAGACATA CTTACAAATG 
     2941 ATGATGTGAA ACTTGATTGG ATGTTCAAAT CATCACTACT GCTTGACCTC ATCAAGGGGA 
     3001 TGAAATACTT ACACCACAGA GAATTTATTC ACGGGAGGCT AAAGTCTCGG AACTGTGTGG 
     3061 TAGATGGGCG TTTTGTCCTA AAAGTGACAG ATTATGGCTT TAATGACATT TTAGAAATGC 
     3121 TGAGACTCTC TGAAGAGGAA CCTTCTGAAG AAGAGCTGCT GTGGACGGCC CCTGAACTGT 
     3181 TAAGAGCCCC TGGGGGCATC AGGTTAGGTT CATTTGCAGG AGACGTCTAT AGCTTTGCCA 
     3241 TCATCATGCA AGAAGTGATG GTTCGGGGTG CTCCTTTCTG CATGATGGAT CTGCCTGCCA 
     3301 AAGAAATCAT AGACAGACTT AAAATGCCTC CCCCTGTGTA CAGACCCGTG GTTTCTCCTG 
     3361 AGTATGCCCC TGCAGAATGT CTCCAGCTGA TGAAGCAGTG CTGGGCTGAG GCGTCAGAAC 
     3421 AGCGACCAAC TTTTGATGAA ATATTTAACC AGTTTAAAAC TTTCAACAAA GGGAAAAAGA 
     3481 CTAATATTAT TGATTCTATG CTTCGGATGT TGGAGCAGTA TTCTAGCAAC TTGGAAGATT 
     3541 TGATCCGGGA ACGGACTGAA GAATTGGAAA TTGAAAAACA GAAAACGGAA AAGCTTCTAA 
     3601 CACAGATGCT ACCACTATCA GTTGCTGAAT CCCTCAAGAA GGGCTGTACA GTTGAACCTG 
     3661 AGGGATTTGA CTTGGTCACC TTGTACTTCA GTGACATTGT GGGCTTCACC ACCATCTCAG 
     3721 CCATGAGTGA GCCCATTGAA GTTGTGGACC TTCTGAATGA CCTGTACACA CTCTTTGATG 
     3781 CCATCATTGG CAGTCATGAT GTCTATAAAG TAGAGACCAT TGGAGATGCC TACATGGTGG 
     3841 CCTCAGGCCT TCCCAAGAGG AATGGCAGTA GGCATGCAGC TGAGATTGCA AACATGTCCC 
     3901 TGGATATCCT GAGTTCTGTG GGTACCTTTA AGATGCGACA CATGCCAGAG GTGCCAGTCC 
     3961 GAATTCGAAT AGGCCTGCAC TCGGGGCCTG TTGTTGCTGG AGTGGTGGGC CTCACCATGC 
     4021 CCAGATATTG CTTGTTTGGG GACACTGTGA ACACAGCTTC TCGAATGGAA TCAACAGGGC 
     4081 TACCATACCG AATTCATGTC AGTCTCAGCA CAGTAACAAT TCTTCAAACT CTGAGTGAAG 
     4141 GCTATGAAGT AGAGCTTCGT GGAAGAACAG AGCTCAAGGG CAAAGGCACA GAGGAGACCT 
     4201 TTTGGCTTGT TGGAAAAAAG GGTTTCACCA AACCTCTTCC TGTACCCCCA CCAGTGGGCA 
     4261 AAGATGGGCA AGTGGGCCAT GGCTTGCAAC CAGCAGAGAT TGCAGCATTC CAAAGGAGAA 
     4321 AAGCAGAGAG GCAGTTGGTG CGAAACAAGC CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4381 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4441 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4501 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4561 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4621 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4681 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4741 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4801 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4861 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4921 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4981 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5041 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5101 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5161 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5221 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5281 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5341 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5401 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5461 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5521 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5581 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5641 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5701 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5761 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5821 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5881 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5941 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6001 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6061 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6121 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6181 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6241 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6301 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6361 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6421 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6481 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6541 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6601 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6661 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6721 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6781 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6841 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6901 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6961 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7021 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7081 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7141 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7201 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7261 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7321 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7381 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7441 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7621 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7681 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7741 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7801 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7861 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7921 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7981 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8041 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8101 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8161 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//