LOCUS       MR220127                8601 bp    DNA     circular  SYN 
DEFINITION  Stag3 (Myc-DDK-tagged) - Mouse stromal antigen 3 (Stag3)
ACCESSION   NM_016964
VERSION     NM_016964.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4758..4849 
                     /label="Myc-DDK"  
     misc_feature    4879..5464 
                     /label="PolyA signal"  
     misc_feature    4936..4955 
                     /label="Restriction Enzyme"  
     misc_feature    5740..6412 
                     /label="ColE1"  
     misc_feature    6842..7636 
                     /label="Kan/Neo"  
     misc_feature    7671..7973 
                     /label="SV40 ori"  
     misc_feature    8059..8536 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4740..4746 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4740 
                     /label="Stag3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTACTCTG TGGTCACCTT CTACCCAGCA CCATGGCTCT TCCTCAGGCA 
     1081 GTGAGTCTTC CCCACTTCAA AAGTCTGTGA GACGTGCACA GATGGCCTTG TCTCCTTGTT 
     1141 CCTCCTCCAT CCTACCCTGT GATGACAGAG ACTCACAGGG AACTGCAGAG TGGGATAGTC 
     1201 CCTCAACTAA CGAAGACAGC GACTTTGAAG ACAGCTTAAG ACGAAATGTG AAGAAGAGAG 
     1261 CAGCAAAGCA ACCACCCAAA GCTGTTCCAG CAGCAAAACA TCGGAAGAAG CAGTCCCGAA 
     1321 TAGTATCTAG TGGGAATGGC AAGAATGAAT CAGTGCCATC AACCAATTAC CTTTTTGATG 
     1381 CTGTGAAAGC TGCTAGAAGT TGCATGCAGT CTTTGGTGGA TGAGTGGCTA GATAACTACA 
     1441 AGCAAGATGA AAATGCAGGA TTCTTGGAGC TCATTAATTT TTTCATCCGA GCCTGTGGAT 
     1501 GTAAAAGCAC TGTGACTCCT GAGATGTTCA AGACAATGTC CAATTCAGAG ATCATCCAAC 
     1561 ACCTAACGGA AGAGTTTAAT GAGGACTCGG GGGACTATCC CCTGACAGCT CCAGGTCCCT 
     1621 CCTGGAAGAA GTTCCAGGGA AGCTTCTGTG AGTTTGTGAA GACATTGGTC TATCAGTGCC 
     1681 AGTACAGTCT CCTCTATGAT GGCTTTCCTA TGGATGACCT TATCTCCCTG CTCATTGGCC 
     1741 TCTCAGATTC CCAGGTCCGA GCCTTTCGTC ATACTAGTAC CCTGGCTGCC ATGAAGCTAA 
     1801 TGACTTCTCT GGTAAAAGTT GCACTCCAGT TGAGTCTGCA CAAAGACAAC AATCAACGTC 
     1861 AGTATGAGGC TGAACGAAAC AAGGGGCCAG AGCAGAGAGC ACCGGAACGA CTGGAGAGTC 
     1921 TGCTGGAGAA ACGAAAAGAG TTCCAAGAGA ATCAAGAGGA CATAGAGGGG ATGATGAATG 
     1981 CCATCTTCAG AGGTGTCTTT GTCCATCGGT ACAGGGACAT CCTTCCTGAG ATCCGCGCTA 
     2041 TCTGCATTGA GGAGATTGGG TATTGGATGC AAAGCTACAG CACCTCCTTT CTTAATGACA 
     2101 GCTACCTAAA ATACATCGGC TGGACCCTGC ATGATAAGCA CAAGGAAGTT CGCCTGAAGT 
     2161 GTGTGAAGGC TCTGGCAGGG CTGTACAGCA ACCAGGAGCT GAGCTTACGG ATGGAGCTCT 
     2221 TTACAAATCG CTTCAAGGAC CGGATGGTTT CCATGGTCAT GGACAGAGAG TGTGAAGTAG 
     2281 CAGTGGAGGC CATCAGATTG CTGACCCTTA TTCTGAAGAA CATGGAGGGA GTGCTGACTA 
     2341 GTGCAGACTG TGAGAAAATT TACTCCATTG TATACATTTC TAATCGTGCT ATGGCCTCTT 
     2401 CTGCAGGGGA ATTTGTGTAT TGGAAGATCT TCCATCCTGA ATGTGGGGCA AAAGCAGTGA 
     2461 GTGATAGAGA GCGACGCCGG AGTCCACAAG CCCAGAAGAC TTTCATTTAT CTTTTACTGG 
     2521 CCTTCTTTAT GGAGAGTGAG CATCACAACC ATGCTGCTTA CTTAGTAGAC AGCTTGTGGG 
     2581 ACTGTGCGGG GTCTTACCTG AAGGACTGGG AGAGTCTGAC AAACCTGTTG CTGCAGAAAG 
     2641 ACCAGAATCT GGGTGATATG CAAGAGAGAA TGCTGATAGA AATCCTTGTG TCTAGTGCCC 
     2701 GGCAAGCTGC AGAGGGTCAC CCTCCAGTGG GGCGCATCAC TGGAAAGAAG AGTCTGACGG 
     2761 CCAAAGAACG CAAGCTTCAA GCCTATGATA AGATGAAGCT GGCTGAGCAC CTCATCCCCC 
     2821 TCTTGCCCCA GCTCCTTGCC AAGTTCTCAG CAGATGCAGA GAATGTTGCT CCCTTGCTCC 
     2881 AGCTGCTCAG TTACTTTGAC CTCAGCATAT ATTGCACTCA GCGCTTGGAA AAGCACTTGG 
     2941 AGCTGCTTCT GCAACAACTC CAGGAGGTGG TGGTGAAGCA TGTAGAGCCT GAGGTGCTTG 
     3001 AGGCAGCAGC CCATGCCCTC TATCTGCTCT GCAAACCAGA GTTCACCTTC TTCAGCAGAG 
     3061 TGGACTTTGC CAGAAGCCAA TTAGTAGATT TTCTGACTGA TAGATTCCAG CAGGAGCTTG 
     3121 ATGACCTAAT GCAGTCATCC TTCCTAGATG AGGATGAGGT ATACAGCCTG ACAGCCACCC 
     3181 TGAAGCGTCT CTCTGCCTTT TACAATGCTC ATGACCTGAC CCGATGGGAG ATCTCTGAAC 
     3241 CATGTTCTCG ACTCCTCCGG AAGGCTGTAG ACACAGGAGA AGTTCCTCAC CAGGTGATTT 
     3301 TGCCAGCCTT GACTCTGGTA TATTTTTCCA TTCTCTGGAC AGTAACCCAC ATTTCAGAGT 
     3361 CTACTTCTCA TAAGCAGCTG ATGAGTCTGA AGAAAAGAAT GGTAGCCTTC TGTGAGCTTT 
     3421 GCCAAAGCTG CCTCTCAGAC GTGGACCCAG AGATCCAGGA GCAGGCTTTT GTCTTATTAA 
     3481 GTGACCTGCT TCTCATCTTC AGCCCTCAGA TGATTGTAGG GGGACGGGAT TTCCTTAGGC 
     3541 CTCTTGTCTT TTTTCCGGAA GCTACTCTCC AGTCGGAACT AGCCAGCTTC CTCATGGACC 
     3601 ATGTCTTTCT CCAGCCTGGA GAACTGGGCA ACGGTCAGTC ACAGGAGGAT CACGTCCAGA 
     3661 TAGAACTTCT GCACCAGAGG CGCCGCCTGC TTGCAGGATT TTGTAAGCTG CTGCTTTATG 
     3721 GGGTATTGGA GCTGGATGCA GCCTCAGACG TTTTCAAACA CTACAACAAG TTCTATGAAG 
     3781 ACTATGGTGA CATTATCAAG GAAACATTAA CTCGAGCAAG ACAAATTGAC AGATGTCAGT 
     3841 GCTCTCGGAT CCTGCTCCTG AGCCTAAAGC AGCTCTACAC AGAACTGATA CAGGAGCAGG 
     3901 GCCCCCAGGG CCTGACAGAA CTGCCAGCCT TCATTGAGAT GAGAGACTTG GCTCGGAGGT 
     3961 TTGCCTTGAG CTTTGGACCC CAGCAGCTCC ATAACCGAGA TCTTGTGGTC ATGCTGCACA 
     4021 AGGAAGGCAT CAAGTTCTCA TTGTCTGAGC TTCCTCCTGC TGGTTCTTCT CATGAGCCCC 
     4081 CAAATCTTGC ATTCCTGGAG CTTCTTTCAG AGTTCTCCCC TCGCCTCTTC CATCAGGACA 
     4141 AGCGGCTACT ACTATCCTAC CTGGAAAAGT GTCTGCAGCG TGTCTCCAAG GCACCTAACC 
     4201 ATCCCTGGGG TCCAGTCACC ACCTACTGCC ACTCCCTTCA CCCTCTAGAG ATCACAGCAG 
     4261 AGGCCAGCCC TCGTGGACCC CCCCACTCCA AGAAGAGGTG TGTTGAAGGC CCCTGCAGGC 
     4321 CTCAGGAAGA AGAGTCCTCA TCCCAGGAAG AAAGCCTTCA GCTGAACAGT GGCCCCACAA 
     4381 CCCCTACCCT CACCTCCACC GCAGTGAAGA GGAAGCAGTC TCTGAGGACA GTGGGCAAGA 
     4441 AGCAAAAAGG TAGACCAGGA CCAGGACCAG GACCAGGACC AGAGCTGATC TGCAGTCAGC 
     4501 AACTCTTAGG CACCCAGAGG TTGAAGATGT CGAGTGCACC ATGTTTCCAG ATTCGATGTG 
     4561 ATCCTTCAGG CTCTGGCTTG GGCAAGCAGC TGACCCGACT CAGCCTTATG GAAGAAGATG 
     4621 AGGAAGAAGA GCTAAGACTT CTGGATGAAG AATGGCAACG TGGAGACAAG ATGCTTCATA 
     4681 GCCCTTCTTC TCCCAGTGAG CATGGGTTGG ACCTATTAGA TACAACAGAG CTGAACATGG 
     4741 AGGATTTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4801 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4861 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4921 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4981 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5041 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5101 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5161 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5221 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5281 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5341 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5401 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5461 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5521 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5581 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5641 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5701 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5761 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5821 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5881 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5941 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6001 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6061 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6121 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6181 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6241 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6301 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6361 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6421 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6481 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6541 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6601 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6661 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6721 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6781 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6841 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6901 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6961 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7021 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7081 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7141 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7201 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7261 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7321 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7381 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7441 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7501 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7561 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7621 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7681 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7741 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7801 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8041 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8101 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8161 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8221 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8281 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8341 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8401 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8461 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8521 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8581 CAAAAATTTA ACGCGAATTT T
//