LOCUS       MR220090                8751 bp    DNA     circular  SYN 
DEFINITION  Mcm9 (Myc-DDK-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9)
ACCESSION   NM_027830
VERSION     NM_027830.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4908..4999 
                     /label="Myc-DDK"  
     misc_feature    5029..5614 
                     /label="PolyA signal"  
     misc_feature    5086..5105 
                     /label="Restriction Enzyme"  
     misc_feature    5890..6562 
                     /label="ColE1"  
     misc_feature    6992..7786 
                     /label="Kan/Neo"  
     misc_feature    7821..8123 
                     /label="SV40 ori"  
     misc_feature    8209..8686 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4890..4896 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4890 
                     /label="Mcm9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCAGAGA ACTACACGAA ATGGAAAATA TTGTGACGTG GAACCGGTGT 
     1081 CCCGCTCAAA CCCCGCCCCA TGCCTCCGAG ACCCGCCCCT CAGACGTCTT GTCCGGCCCA 
     1141 AACCCCGCCT CCAGCTCCCC GAGTCCCGCC TCTCTCCCTG CTCCCGCCTC CCGCTCGCAG 
     1201 ACTCCAGCGT CCGTCCTGGC GCGCGCCCGC CTGCGTCCGC ACCCGGAAGA AGCCCCAGCG 
     1261 GCCGGAAAGT TGAGGCAGTG CGCGGCTCGG GGTCCGCGGG AAGCTCAAGT CCTTCAGAGG 
     1321 CCGAGCGAGA GCAGCGCGAA GAAGCCTGCG CGCCCCCGCG CAAGGCGGCC CCCTCGAGCG 
     1381 GCCGCGCGCA CGCCCCGCCC CCTCCAACGC CGCGCGGGTC GGGCTGGGGC GACCACGGCC 
     1441 GCAGCGCGGT CCCGGCGACC AAGACAGTGC GTGTTGAGCC CTACCCACCC TTCAAGATGA 
     1501 ATAGTGAGCA GGTCACCCTG GTGGGTCAGG TGTTTGAGTC CTATGTTTCA GAGTACCATA 
     1561 AGAACGATAT TCTTCTGATC CTGAAAGAAA GAGATGAAGA TGCTCACTAC CCGGTTGTGG 
     1621 TTAATGCTAT GAGCCTTTTC GAGACCAACA TGGAAATTGG GGACTATTTC ACCGTGTTCC 
     1681 CCAATGAAGT ACTAACAGTT TTTGACAGTG CACTTCGAAG GTCAGCCTTG GCAATTCTGC 
     1741 AGTCCCTTCC TGAGACGGAG GGGTTATCCA TGAAGCAGAA TCTTCATGCC AGGATATCAG 
     1801 GTTTGCCTGT TTGTCCAGAA CTGGTCAGGG AACACATTCC CAAAACCAAG GATGTGGGAC 
     1861 ACTTCTTATC TGTCACTGGG ACAGTGATCC GAACGAGTCT GGTGAAGGTC TTGGAGTTCG 
     1921 AGCGGGATTA CATGTGTAAC AAATGCAAGC ATGTGTTCAT GGTGGAGGCA GACTTCGAGC 
     1981 AGTATTACAC CTTCAGTCGG CCATCGTCAT GTCCAAGTTT AGCCAGCTGT GACTCCTCAA 
     2041 AATTCTCTTG CCTCTCAGAC TTGTCTTCAT CTCCAGCCAG ATGTCGGGAT TACCAGGAAA 
     2101 TCAAAATTCA GGAGCAGGTG CAAAGGCTGT CTGTTGGAAG TATCCCACGG TCTATGAAAG 
     2161 TTATTCTGGA AGATGACCTA GTTGACAGTT GCAAATCTGG AGATGACCTC ACCATCTATG 
     2221 GGGTTGTAAT GCAACGGTGG AAACCCTTTC AGCGAGATGT ACGCTGTGAA GTTGAGATTG 
     2281 TCTTGAAAGC CAACTATGTC CAAGTGAATA ATGAGCAATC CTCGGGGATG GTCATGGATG 
     2341 AGGACACTCG AAAAGAATTT GAAGACTTCT GGGAACACTA TAAGAGTGAC CCCTTTGCAG 
     2401 GGAGGAATGA AATATTGGCC AGCTTGTGTC CTCAAGTTTT TGGGATGTAT CTAGTGAAGC 
     2461 TTGCTGTGGC CATGGTACTG GCTGGTGGAA TTCAAAGAAC TGATGCTGCA GGAACCAGGG 
     2521 TTAGAGGGGA ATCTCACCTT TTATTGGTTG GGGATCCTGG CACAGGGAAA TCACAATTCC 
     2581 TTAAATATGC AGCAAAGATT ACCCCAAGGT CCGTTTTGAC CACAGGAATT GGATCTACTA 
     2641 GTGCAGGTCT GACCGTGACA GCTGTCAAAG ACTCAGGAGA ATGGAATCTA GAGGCTGGGG 
     2701 CTTTGGTGCT TGCAGATGCT GGTCTCTGCT GTATTGACGA ATTTAACAGC CTCAAAGAAC 
     2761 ATGACAGGAC AAGCATCCAT GAAGCAATGG AGCAACAAAC CATAAGTGTT GCTAAGGCTG 
     2821 GCCTTGTTTG TAAGCTGAAC ACAAGGACCA CCATCCTGGC AGCAACTAAC CCCAAAGGCC 
     2881 AGTATGACCC CAAGGAGTCT GTGTCTGTGA ACATTGCCCT CGGGAGCCCA CTCTTAAGTC 
     2941 GATTTGACCT TGTCCTGGTT TTGCTCGACA CTAGGAATGA AGACTGGGAT CGTATCATTT 
     3001 CTTCCTTTAT CTTAGAAAAT AAAGGTTATC CAAGCAAATC AGAGAATCTG TGGAGCATGG 
     3061 AGAAGATGAA AACCTACTTC TGCCTCATTC GGAATCTCCA CCCCACACTG TCTGAAGTGA 
     3121 GCAATCAAGT CCTCCTTCGA TACTACCAAA TGCAAAGGCA GAGTGATTCC CGGAATGCAG 
     3181 CCCGGACAAC CATCCGCCTG TTAGAAAGCT TGATCCGATT AGCAGAAGCT CACGCTCGCC 
     3241 TGATGTTCCG CAGTGCCGTG ACTCTGGAAG ATGCCGTTAC AGCTGTATCT GTGATGGAGT 
     3301 CTTCAATGCA GGGAGGTGCT CTGCTAGGAG GTGTGAATGC TCTCCACACT TCCTTCCCTG 
     3361 AAAACCCCCG TGCACAGTAC CAGAGGCAGT GTGAACTCAT TCTGGAAAAG CTGGAACTCC 
     3421 AGGGCCTCTT GCAGGAAGAA CTTCGAAGAC TGGAAAGGTT ACAGAATGAG AGTGTACACC 
     3481 AATGCCAGTC ACATTCACTA GAGGAGGAGG TGGCTCCAGG TTCCTGCAGA AATGATCCCA 
     3541 GGGACAAGCC AAGGCTCAGG ACTTCAACAC AGCAGGAACA GAGCTGTAGC TGGAGTTCCA 
     3601 CAGAGAGATC TGGTGCAGAC TCCCCGCCTG GTCCAGGGCT TAATAGACCA ACAAGTTGTA 
     3661 ACAACTCAGC TGAGAACAGA GATGGCAGAG GTGACGGTTT AGACTGGTTG GACCCCACAT 
     3721 CAAGTCCTGA GATTGCACCA GAAAGCACTA TTGTGTCTCC CAATGTGAAA ACAACTGAGA 
     3781 AAAATGTGAA TTTGAAAATC TCCAACAATA AATCTCAGGG CAAGGAGAAG CATGGGCCAC 
     3841 AGCAAAGAAG CAAATTATTA GAAGCTGGAC ATCTTCCATC ATCAGGAGCC ATGAATGCCC 
     3901 CCTTACGGTC TCACGGTGTT AAGCGTACAA AGGCAAGTCA GGCAGTGGTT GTATCTGAAG 
     3961 CAGGACGGGG TGATGAAGAA GACTCTGTGC CCCGAAGACT CCCCAAGCTG CTGAAGGAGG 
     4021 GGTCACAGAA TGTGTGCAGA AGCACAACCA GAGTGCGACC ACTGCCACCC ACTGTCCCTC 
     4081 TGTCCCTGTC TATCCCTTCA CCTGGGTCAG GAAAAAGATC AGGAACACCC AAAAGAAAGA 
     4141 GACGGAAATC TGCTCAGGTG GAAGAGCCTG AACCTGAAGG TATGGAGACT CCAACAGTAA 
     4201 AGCTGGCCAA ATTCACATTT AAACAGAAGA CAAAACTGAC CCACTCCCCC GAAGGCCAAG 
     4261 GCCCCATACC TCCCAGTGCT AGTGAAATAG CAGTTGACAG TTCTAAAATT CCCCAACAAA 
     4321 GAACAAGAAG AGAAGCAGCT GTGCCTGTGG TAGCGCCAGG AAAGTCGACA TCCACCTCAG 
     4381 GAGACAGATG CTCTGACCAG CTGCACGGAA AGACAAAGGA GCTCTCAAGG CAGCCACCAG 
     4441 ACAGTAATCC ACCAAGAGAG GAGAGGGAAC AGGGCCCCAA AAGAAGGGTT ATTCAGCCCA 
     4501 AACCGGAGCT TGGGAACCAG GCTGGGCATT CGCATCTAGC CTGTGAGAAA GACAGAAAGG 
     4561 AAGGGGTTTC CTGCGGTAAT AAAAGCAGCA AGGTTCATGC TGGCACCATA GCCAGACTGG 
     4621 CAAGCTTCTC CTTCACTTCC CCATCAGAAT CCAAATCGGA ATCCCTCCCT CCTGAAAGGA 
     4681 AGGACAGCAG GGACAGCAGG GACAGCAGGG ACAGCAGGGA CAGGTGCCAC AGCCCTCCTG 
     4741 CGACCACAGC TCCAGTGCTC GGCCAGCAAA GGCAGACTTT CCAGCTGCAG CAGCCCACAG 
     4801 AGAGAGCGAA TCTTTCCACC CTCTCCCTCT TCACTCTGTC GGAACTAGAT GACGAAGCAT 
     4861 TAGATTTTGA CTGGGAGGAA GAGATGAGGA AAAAGCCAAC GCGTACGCGG CCGCTCGAGC 
     4921 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4981 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5041 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5101 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5161 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5221 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5281 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5341 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5401 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5461 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5521 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5581 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5641 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5701 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5761 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5821 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5881 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5941 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6001 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6061 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6121 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6181 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6241 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6301 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6361 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6421 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6481 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6541 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6601 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6661 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6721 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6781 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6841 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6901 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6961 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7021 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7081 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7141 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7201 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7261 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7321 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7381 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7441 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7501 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7561 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7621 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7681 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7741 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7801 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7861 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7921 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7981 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8041 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8101 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8161 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8221 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8281 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8341 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8401 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8461 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8521 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8581 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8641 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8701 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//