LOCUS       MR220029                8526 bp    DNA     circular  SYN 
DEFINITION  Vwa5b1 (Myc-DDK-tagged) - Mouse von Willebrand factor A domain containing 5B1 (Vwa5b1)
ACCESSION   NM_029401
VERSION     NM_029401.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4683..4774 
                     /label="Myc-DDK"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    4861..4880 
                     /label="Restriction Enzyme"  
     misc_feature    5665..6337 
                     /label="ColE1"  
     misc_feature    6767..7561 
                     /label="Kan/Neo"  
     misc_feature    7596..7898 
                     /label="SV40 ori"  
     misc_feature    7984..8461 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4665..4671 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4665 
                     /label="Vwa5b1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGGCCTG CTGAACTGCC TCACAGGGGC AGCCCTGCCT CTCATGGAAT 
     1081 CCGATGTCAC CTCCTATGTC AGTGGCTATG CCCTGGGCCT GACAGCCTCC CTCACCTACG 
     1141 GCAACCTGGA GGCCCAGCCC TTTCAAGGTC TCTTCGTGTA TCCAATAGAC GAGTATAGCA 
     1201 CAGTGGTTGG CTTTGAAGCA GTCATTGCTG ACCGTGTTGT GACCATACAG CTCAGAGACA 
     1261 AAGCCAAGCT GGACAGAAGC CACTTGGATA TCCAACCCGC CACTGTCACA GGGAACTTTC 
     1321 CGGAAGAGGA GAGTCCCATC GCACCAGGAA AGGTGACCTT GGATGAGGAT CTGGAGCGGG 
     1381 TTCTGTTCGT GGTCAACCTG GGGACCATTG CCCCCATGGA GAATGTCACC GTCTTCATCA 
     1441 GCACCTCCTC CGAACTCCCC ACACTGCCCA GCGGGGCTGT GAGGGTTCTT CTGCCGGCCA 
     1501 TCTGCGCCCC GACCGTGCCT CCATCCTGTA CCCACAGGTT TGGCTCCTCC AGCCCACAGC 
     1561 CACAAGGCAA AGACCCACAC TGCTTCGGCA CCCAGACCAA GGACTCCTAT AACAGGCTGT 
     1621 GTCTGGCCAC TCTCCTGGAC ACAAAGGTGA CCAACCCAAT GGAATATGAA TTCAAATTTC 
     1681 AGCTGGAAAT CCGTGGGCCC TGTCTGCTCG CAGGGGTGGA GAGTCCCACT CATGAAATCC 
     1741 GAGCAGACGC AGCCCCATCG GCACACTCCG CCAAGAGCAT CATCATCACG TTGGCCAAAA 
     1801 AACATACCTT TGACCGGCCA GTGGAGATTC TTCTCCACCC TAGCGAGCCT CACATGCCAC 
     1861 ACGTCCTGGT GGAGAAAGGG GACATGACCC TGGGGGAATA TGACCAGCAC TTGAAGGGAA 
     1921 AAGCAGATTT CATTCGGGGG ACAAAGAAGG ACAACAGCGC GGAGCGGAAG ACAGAAGTCA 
     1981 TCCGGAAGCG TCTCCACAAA GACATTCCCC ACCACTCAGT CATCATGCTC AACTTCTGTC 
     2041 CTGACCTCCA GTCTGTGCAG CCCAACCCGA GGAAAGCCCA CGGGGAGTTT ATCTTCCTCA 
     2101 TTGATAGGAG TAACAGCATG AGCAAGACCA ACATCCAGTG CATCAAGGAG GCCATGCTGG 
     2161 TGGCCCTCAA GAGCCTCATG CCAGCGTGCT TCTTCAACAT CATTGGTTTT GGATCCACAT 
     2221 TTAAGGCAGT CTTTGCTTCC AGTCGGATCT ACAATGAGGA GAACTTGACC ATGGCATGTG 
     2281 ACTGCATCCA GAGAATGCAG GCTGATATGG GAGGCACCAA CATGCTCTCC CCCCTCAAGT 
     2341 GGGTCCTGCG GCAGCCACTG CGCCGGGGTC ACCCACGGCT CCTCTTCCTG ATCACGGATG 
     2401 GGTCCGTGAA CAACACAGGG AAGGTTCTGG AGCTGGTACG GAATCACGCC TCTTCTACCA 
     2461 GGTGCTATAG CTTTGGAATT GGACCCACGG TCTGCTACCG ACTGGTCAAA GGGCTGGCAT 
     2521 CTGTATCCAA GGGCAGCGCA GAGTTCCTGA TGGAGGGGGA ACGGCTGCAA CCAAAGATGG 
     2581 TCAAATCCTT GAAGAAGGCC ATGGCGCCAG TGCTGAGTGA CGTGACTGTG GAGTGGGTCT 
     2641 TTCCCGAGAC CACGGAGGCC CTGATCTCTC CTGTCAGCAC CAGCTCCCTT TTCCCTGGGG 
     2701 AACGGCTGAT GGGATATGGC ATTGTGTGTG ATGCCTCCTT GTACATTTCC AATTCCAGAT 
     2761 CTGACAAAAG GCGAAAGTAT GGCATGCTGC ACACTCAGGA GTCCAGCAGC TCCGTCTTCT 
     2821 ACCCCTCACA GGACGAGGGA CTCAGCCCAG GCAGTGGAAA CTGTGCCAAG AACGTCAACC 
     2881 AGGGGCAGAC CAAAGATGCC CATCCATGCA ATGGAGACTC CCCCACCCAT CACGGTCTGG 
     2941 ATGTCTCCCG GCGGCGGAGA GCATACAGCA CTAACCAGAT CTCCAGCCAC AAGACTTGCC 
     3001 CAAGGGCTAC CACAGCCAGC GACCCCACCG GGACTGCCAG GAGATACCCA CTGCGAAAAG 
     3061 CCAAGGTGCA AGACCTTGCC AGTGAGAGCG ACTGGGAGAG CCAGAAGTGG CAGACTGATC 
     3121 TGCAGACCCT ACTTAACGAA GGCCATAACC TGAGCCAGGG CCCCAAACTT CATGGCCCAG 
     3181 GAGCCCGCAG GCCTTCCCTA CTGCCTCAAG GCTGCCAGCT CATGCGCTTC TTTGACCAGA 
     3241 AGCCCCAGGC CTGGGGCCCT GTGAGAGAGC TGGATTGTGG TGCCAGCCGG ACGTCTGCCC 
     3301 CTAACAGCCA AAGCTCTGAA GATCTGGCCA TAGAGCCAGC CCATTGTCCT TCAACCTTTG 
     3361 AACGGGAGAC ATCCTTAGAC TTGGAGCCCA TGGCTGAGTC CGAGGAGCAA GCCAATCCCT 
     3421 GCAGGACTGC CACTCCAAGC CCTGTGGTGG GCAAGGCTTT GGTCAAAGGC CTGTGCGCCA 
     3481 ACCAACGCAT GCAGTGGGAA GTGAGCTTCG AGCTGGAGCC TCCAGCCCTG AAGAGGGGGG 
     3541 ACACACAGAA CGCTGACATG TGGAGTGAGA CTTTCCACCA CCTGGCGGCC CGCGCCATCA 
     3601 TCCGAGAATT CGAGCATCTG GCAGAGCGCG AGGACGAGAT CGAGCTGGGG TCCAATCGCC 
     3661 GATACCAGGT GAACGCCGTG CACACCAGCA AGGCCTGCAG CGTGATCAGC AAATACACGG 
     3721 CCTTTGTGCC TGTGGACATA AACAAGAGGC AGTACCTGCC CACGGTCGTC AAGTACCCCA 
     3781 ACTCTGGTGC CATGCTCAGC TTCCGGAACC TGACTCGACA GTGGGGGGGC TCTTCGGCTG 
     3841 GCCTGGGAAG GCCCCAGTCT ATGCTCAGAG AACACTCCTC AGCCGCAGGA GACAGCAAAT 
     3901 TCCAGACTCT AGCCCTACAA GACAGCCCTA CTTCAACCTT CAATAAAACC CCATCCCCCG 
     3961 GCCATGAGAA GCAAACCACG GCAGAAGGTC CCCCTCAAAA CCTGTCCGCC AGCGCTCCAT 
     4021 CCTCCATGAA GGCCACTGAG ACTCTCTTTG GATCTAAGTT GAATCTCAAC AAATCCAGGC 
     4081 TGCTGACCCG AGCTACCAAA GGCTTTCTCA GCAAATCGTT GCCCAAGGCT TCAGAGGCAA 
     4141 CCCCAGGCAG CCAGAGCTCT GACTACATAC CACTGGTGTC TCTGCAGCTG GCCTCGGGAG 
     4201 CCTTTTTGCT CAATGAAGCC TTCTGCACGA CCATCCAAAT CCCCATGGAG AAACTCAAGT 
     4261 GGACCTCACC CTTCTCCTGC CTCCGAATGT CCCTCGTCAC CCGCCGACAG GATCTGAAGA 
     4321 CCCAGAGTCC ACAGGACTGC ACCAGTCTCT CCTCTTCACC CCCATCCTGT GACGGCATCT 
     4381 CCCTAAAGTC TGAAGAGAGC TCAGACCAGG AGTCCAATGC AATGCTGGAG CACATGGGAA 
     4441 AGCTGTGGGC CACGGTGGTG GCGCTGGCGT GGCTGGAACA CAGCTCAGCT AACTACATCA 
     4501 TTGAGTGGGA GCTGGTGGCT GCCAAGGCCA GCTCCTGGGT GGAGAAGCAG AAAGTTCCTG 
     4561 AGGGCCGCAC CTTGAGCACT CTCAAGAACA CTGCTCGCCA ACTCTTTGTG CTTCTGCGAC 
     4621 ACTGGGATGA GAAATTAGAG TTCAATATGC TCTGTTACAA CCCCAATTAT GTGACGCGTA 
     4681 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4741 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4801 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4861 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4921 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4981 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5041 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5101 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5161 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5221 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5281 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5341 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5401 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5461 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5521 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5581 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5641 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5701 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5761 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5821 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5881 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5941 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6001 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6061 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6121 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6181 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6241 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6301 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6361 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6421 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6481 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6541 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6601 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6661 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6721 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6781 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6841 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6901 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6961 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7021 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7081 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7141 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7201 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7261 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7321 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7381 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7441 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7501 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7561 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7621 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7681 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7741 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7981 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8041 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8101 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8161 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8221 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8281 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8341 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8401 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8461 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8521 AATTTT
//