LOCUS       MR219985                8451 bp    DNA     circular  SYN 
DEFINITION  Mtmr4 (Myc-DDK-tagged) - Mouse myotubularin related protein 4 (Mtmr4)
ACCESSION   NM_133215
VERSION     NM_133215.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4608..4699 
                     /label="Myc-DDK"  
     misc_feature    4729..5314 
                     /label="PolyA signal"  
     misc_feature    4786..4805 
                     /label="Restriction Enzyme"  
     misc_feature    5590..6262 
                     /label="ColE1"  
     misc_feature    6692..7486 
                     /label="Kan/Neo"  
     misc_feature    7521..7823 
                     /label="SV40 ori"  
     misc_feature    7909..8386 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4590..4596 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4590 
                     /label="Mtmr4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGAGGAG GGCCCCCCCA GCCTGGAGTA CATCCAAGCC AAGGATCTGT 
     1081 TCCCCCCCAA GGAACTGGTG AAGGAGGAGG AGAATCTTCA GGTACCCTTC ACAGTGCTGC 
     1141 AGGGTGAGGG AGTGGAATTC CTGGGCCGGG CAACCGATGC CCTCATTGCC ATCTCTAACT 
     1201 ACCGGCTGCA CATCAAGTTC AAAGACTCTG TCATCAACGT GCCCCTCCGG ATGATTGACA 
     1261 GTGTGGAAAG CCGTGATATG TTTCAGTTGC ACATTGCCTG CAAGGACTCC AAAGTGGTGA 
     1321 GGTGCCACTT CTCTACTTTC AAGCAGTGCC AAGAGTGGCT CTCAAGGCTA AGCCGGGCCA 
     1381 CAGCGAGACC TGCCAAGCCC GAGGATCTCT TTGCCTTTGC CTACCATGCG TGGTGCCTGG 
     1441 GGCTGACCGA GGAGGACCAG CATACCCACC TGTGCCAGCC AGGAGAGCAC ATCCGATGTC 
     1501 GACAGGAGGC AGAGCTCGCA AGGATGGGTT TTGACCTGCA GAATGTCTGG AGAGTCTCGC 
     1561 ATATCAACAG CAATTACAAG CTGTGCCCTA GTTACCCCCA GAAGCTGCTG GTTCCTGTGT 
     1621 GGATCACAGA TAAAGAGCTG GAGAACGTGG CTTCTTTCCG CTCTTGGAAA CGCATCCCTG 
     1681 TTGTGGTATA CAGACACCTG CGCAACGGGG CTGCCATTGC TCGCTGCAGC CAGCCTGAGA 
     1741 TCAGCTGGTG GGGCTGGCGC AATGCTGATG ATGAATACCT CGTCACGTCC ATTGCCAAAG 
     1801 CCTGCGCCTT AGACCCAGGG ACAAGGGCCA GTGGGGGCTC CCTCAGCACT GGGACTAATG 
     1861 ATGCCAGTGA GGCATGTGAC ACAGATTTCG ATTCTTCTCT GACTGCATGC TCTGGGGTGG 
     1921 AGAGCACAGC AGCCCCACAG AAACTGCTGA TCCTGGATGC ACGGTCCTAC ACAGCAGCCG 
     1981 TGGCTAACCG GGCCAAGGGT GGAGGCTGTG AATGTGAAGA ATACTATCCG AACTGTGAGG 
     2041 TCTTGTTTAT GGGAATGGCC AACATCCACG CCATCCGGAA TAGCTTCCAG TACCTCCGGG 
     2101 CTGTGTGTAG CCAGATGCCA GATCCCAGCA ACTGGTTGTC GGCACTGGAG AGTACCAAAT 
     2161 GGCTGCAGCA CTTGTCAGTG ATGCTAAAAG CAGCTGTGTT GGTAGCCAAT ACGGTAGATC 
     2221 GGGAAGGCCG GCCTGTGCTG GTACACTGCT CTGACGGCTG GGACCGTACA CCACAGATTG 
     2281 TAGCCCTGGC CAAAATACTA CTGGATCCAT ATTACAGGAC TCTGGAGGGC TTCCAAGTGT 
     2341 TAGTGGAGTC TGATTGGCTG GATTTCGGAC ACAAGTTTGG AGACCGCTGT GGCCATCAGG 
     2401 AAAATGCAGA GGACCAAAAT GAACAGTGTC CTGTGTTCCT CCAGTGGCTT GATTCCGTGC 
     2461 ATCAGCTGCT TAAGCAGTTC CCCTGCCTGT TTGAGTTCAA TGAAGCATTT CTGGTAAAGC 
     2521 TGGTGCAGCA CACATACTCG TGTCTCTACG GCACATTCCT GGCCAACAAC CCCTGCGAGC 
     2581 GAGAGAAGCG CAACATCTAT AAACGGACGT GCTCTGTGTG GGCACTCCTG CGAGCTGGCA 
     2641 ACAAGAACTT TCATAACTTC CTCTATACAC CTGGTTCAGA CGTGGTCCTG CATCCTGTGT 
     2701 GTCATGTCCG GGCCCTGCAC CTCTGGACAG CTGTTTATCT GCCTGCGTCA TCTCCATGCA 
     2761 CCCTTGGAGA GGAAAATATG GATCTTTACC TTTCCCCAGT GGCTCAGAGC CAGGAATTTT 
     2821 CTGGTCGTTC TCTGGACAGG TTACCTAAAA CCAGATCCAT GGATGATCTG CTCTCTGCCT 
     2881 GTGATACAAG CAGCCCCCTC ACTCGTACTT CCAGCGACCC TAACCTGAAT AACCACTCTC 
     2941 AGGAGGTCAG AGGCAGCCTG GAGCCCTGGC ACAGCAGTCC TGAAGGAGCA GAGACAGTCA 
     3001 TAGACTCTGG AGTTGGAAGC CCACAGCTGA CTGTGGGAGA AATGGGCCTT CCTCCTCCTC 
     3061 TGCCCAGTAG CCAGAAAGAG TACTTGAGCA ATAAACCTTT CAAGGGTCAT AAAAGTTGTT 
     3121 CTCTTAGTTA TAAGTTGCTT AATACTTCTG TGTCCTGGGA AATGAAGAGC AACACCTCTG 
     3181 ACATCAAGGT CCTGGAAGAA ACTGAAGCAC TGGCCCCGGA CCCTTCAGCC CAAGAGGAGC 
     3241 AGGGTAGGAC TTCTGATGGC TTAGGGAAGC CACCTGAACA GTTTCTAGAG AAGGAAGCTG 
     3301 TCAGCTCACT CTGCAGTGTC TCTAGCAAGT GTGGTGGAGC CTGTGATTTT CCTGAGCCTC 
     3361 CCCAGGACCC CCTAACAGGT ACTCCCCAAC AGCCCCACCT AGACTCCATG CAGATCTCGC 
     3421 CCTCAAGGTG CACTCCAGAT CACAGTCAGG GCAGCCTTTG CAACCCACCA AGTGTGGCCA 
     3481 GCCAAACTCC AGAGCCAAAT ACTGACCTCC TCAGCCAAGA TCCCCCTGGT TCTACGGCAA 
     3541 GTATCTCCCA CCAGGAACAG CCTAGCTCTG TGCCGGATCT GATCTACAAG AAGGAGGATG 
     3601 CTGGCAAGAG AGGAAGTAAG AATGGGCAGC TGTTGGAAAA TCCTCGCTTT GGAAAAATGC 
     3661 CATTGGAGTT GGCCCGAAAG CCAATTTCTC AGAGCCAGAT CAGTGAGTTC TCATTTTTAG 
     3721 GGTCAAACTG GGACAGCTTC CAAGGGATGA TGACTTCATT CCCAAGCGGA GAGACCACTC 
     3781 CTCGGCGCCT GCTTGCTTAT GGCTGTTGTA GCAAGAGGCC GAGCAATAAG CATATTCGAG 
     3841 CTGCAGGGCC CTGCCTTGGG GGCCAGTGGG CTCAGAGAGA AGGGATGAAA TCTCCTGTCT 
     3901 GTTCTAGTCA TTCCAATGGA CACTGCACAG GCCCAGGAGG GAAGAACAAC CGGATGTGGT 
     3961 TTTCCAGTCA TCCGAAGCAA GTCTCCAGCA CAAAGCCTTC TCTTCTGAGC TGCCCTTCCC 
     4021 CAGTGCCTCC TCTCTACCTG GATGATGATG GACTCCCCTT TCCCACGGAT GTGATCCAAC 
     4081 ACAGGTTACG GCAGATCGAA GCAGGGTACA GGCAAGAGGT GGAACAGCTA CGGCGACAGG 
     4141 TTCGAGAGCT TCAGATGAGG CTAGACATTC GTCATTGCTG TGCCCCTCCA GCAGAACCCC 
     4201 CCATGGACTA TGAGGATGAC TTTACGTGTT TAAAGGAGTC AGATGGCAGT GACACTGAAG 
     4261 ACTTTGGCTC TGATCACAGT GAAGACTGCC TTTCAGAAGC AAGCTGGGAA CCCGTTGATA 
     4321 AGAAGGAGAC TGAGGTGACT CGCTGGGTTC CAGACCATAT GGCATCCCAC TGCTTTAACT 
     4381 GTGACTGTGA ATTCTGGTTG GCCAAACGAA GACATCATTG CAGAAATTGT GGGAACGTAT 
     4441 TTTGTGCTGG ATGCTGCCAC CTTAAGCTGC CCATTCCTGA TCAGCAACTC TATGACCCAG 
     4501 TTCTCGTCTG TAACTCATGT TATGAACACA TTCAAGTATC TCGTGCCAGG GAACTCATGA 
     4561 GCCAACATCT GAAGAAACCC ATTGCTACGG CTTCCAGTAC GCGTACGCGG CCGCTCGAGC 
     4621 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4681 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4741 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4801 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4861 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4921 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4981 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5041 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5101 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5161 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5221 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5281 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5341 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5401 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5461 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5521 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5581 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5641 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5761 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5821 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5881 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5941 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6061 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6121 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6181 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6241 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6301 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6361 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6421 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6481 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6541 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6601 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6661 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6721 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6781 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6841 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6901 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6961 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7021 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7081 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7141 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7201 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7261 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7321 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7381 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7441 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7501 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7561 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7621 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7681 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7861 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7921 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7981 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8041 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8101 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8161 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8221 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8281 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8341 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8401 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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