LOCUS       MR219928                6591 bp    DNA     circular  SYN 
DEFINITION  Exd1 (Myc-DDK-tagged) - Mouse exonuclease 3'-5' domain containing 1 (Exd1)
ACCESSION   NM_172857
VERSION     NM_172857.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2748..2839 
                     /label="Myc-DDK"  
     misc_feature    2869..3454 
                     /label="PolyA signal"  
     misc_feature    2926..2945 
                     /label="Restriction Enzyme"  
     misc_feature    3730..4402 
                     /label="ColE1"  
     misc_feature    4832..5626 
                     /label="Kan/Neo"  
     misc_feature    5661..5963 
                     /label="SV40 ori"  
     misc_feature    6049..6526 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2730..2736 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2730 
                     /label="Exd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCCAGC AGTGACTACC ATTTCCTCAA CCAGATTTTG TGGAGAAGGG 
     1081 TGAAGCTCAC ACTGGTGTCT GGCATCTTTG AGGGCGTGCT CCAGCACGTG GATCCTAACA 
     1141 AGATTGTTGT CCTGAAAAAC GTGAGGAATG CAGAGTCGGG CCGCAGCGTC CCAGGGGTGA 
     1201 AAGTGTTCTT CGGGCACGAG ATCTTGAATG TGGAACTAAT GGATGAAGCA GAAGGTGCAT 
     1261 CGGGGGAGAA GGCATCCGCT GTTAGCATAA ATACAGAACG AGCTGGAATG GAGAAAGTGA 
     1321 AAAATGAAGA CGTCAATGTC TGTGAGCCGG CTTCACCTGC ACCAGAGGTG CCAACCAGCT 
     1381 CTCTCCTCAG TGACCTCAAG TACTGCCCGT CAGAGGAAGA GGAGGTGACA TACACAGTCA 
     1441 TTGATCAGTT CCAGCAGAAG TTTGGTGCTG CAATGCTCCA CATCAAGAAG CAGAGTGTCC 
     1501 TGAGTGTTGC TGCCGAAGGC GCCAATGTGT GTCGCCATGG GAAACTCTGT TGGCTTCAGG 
     1561 TGGCCACAAA CAGCCGAGTT TACTTGTTTG ATATTTTCCT TCTGGGAAGT CGTGCTTTCA 
     1621 ATAATGGACT TCAGATGATC TTAGAAGACA AGAGAATCTT AAAGGTTATC CACGATTGTC 
     1681 GCTGGCTCTC TGACTGCCTG TCTCATCAGT ATGGAATCAT GCTGAACAAT GTCTTTGATA 
     1741 CACAGGTAGC CGACGTCCTT CAATTTTCTA TGGAAACGGG TGGCTTTCTT CCAAACTGTA 
     1801 TCAGTACTTT GCAGGAGAGT TTAATCAGAC ACCTTAAAGT TGCTCCCAGA TACCTTTTCT 
     1861 TTTTAGAAGA GAGACAGAAA CGTATTCAGG AAAATCCAGA AATATGGTTG ACAAGACCTC 
     1921 TTCCACCCTC TTTATTGAAA ATCTTGGCCC TGGAAACAAC CTACCTGCTT CCACTTCGAT 
     1981 TGGTGCTCCT GGATGAGGTG ATGTCAGACT TAACCACACT GGTGGATGGG TACCTGAACA 
     2041 CCTACCGGGA GGGCTCTGCA GACCGGCTTG CAGGCACAGA GCCTGCGTGT ATGGAGCTGC 
     2101 CAGCAGAGCT TCTTCAACTC CAGGACTTCC AGAAACAGCG CAGAGAGCGA GCTGTGAAGG 
     2161 AGTACAGAGT GAACGCACGG GGCCTCCTCA TAAGGACTCC ACTGCACCCA AAGGAGCCGA 
     2221 CAGCTTGCAC AGCAGGGAAA GAGGAGCGAG TCCAGGGTTT CTTATTTTAT AAGACGGATG 
     2281 GTGGAGACCA AGTTCCACGC TTTTTATGTC CCAAATCTCA TGAGGATGAG AAGTTTTTGG 
     2341 ATAAAGAATC TAAACAAACC ACAGCAAAGT CTCAGATTGT GCCTCCCAGG AAGGAAGGCG 
     2401 AAGCCCATAA GGACTCTAAG AACAAACCGG GGTGTTGGGA GTCAGCGGGG CCAGAAGACC 
     2461 CGAGAGCCCA GAAAGCCCAC GCCCTGCCTC CCACGTGGGC ATCTCAGTCC CAGTTTTCTT 
     2521 TGAAAGAGGA GATAGAGCAG TTGACAGTGG TGGGAAATAA GGGAGCTTTA ACAAGCCCAA 
     2581 AAGAAGGCGC TTTGGTGTCG CCTTCTCTCC TTCAGGAAAC CTGGGAGGCA CCAACTGACA 
     2641 CCTTCCATCT TCCTGAAAAA GCTGAGGTCT CTACACTTCC TCCCTGTCCA GCCCTGGAGA 
     2701 AGACAGACTC ATGGATAAGT CCATCTTTAA ATCTGTTTAC GCGTACGCGG CCGCTCGAGC 
     2761 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     2821 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     2881 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     2941 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3001 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3061 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3121 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3181 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3241 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3301 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3361 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3421 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3481 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3541 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3601 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     3661 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     3721 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     3781 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     3841 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     3901 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     3961 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4021 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4081 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4141 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4201 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4261 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4321 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4381 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4441 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4501 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4561 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4621 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     4681 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     4741 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     4801 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     4861 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     4921 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     4981 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5041 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5101 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5161 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5221 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5281 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5341 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5401 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5461 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5521 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5581 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     5641 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     5701 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     5761 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     5821 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     5881 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     5941 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6001 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6061 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6121 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6181 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6241 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6301 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6361 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6421 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6481 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6541 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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