LOCUS       MR219895                7224 bp    DNA     circular  SYN 
DEFINITION  Spg7 (Myc-DDK-tagged) - Mouse spastic paraplegia 7 homolog (human) (Spg7), nuclear gene encoding mitochondrial protein
ACCESSION   NM_153176
VERSION     NM_153176.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3381..3472 
                     /label="Myc-DDK"  
     misc_feature    3502..4087 
                     /label="PolyA signal"  
     misc_feature    3559..3578 
                     /label="Restriction Enzyme"  
     misc_feature    4363..5035 
                     /label="ColE1"  
     misc_feature    5465..6259 
                     /label="Kan/Neo"  
     misc_feature    6294..6596 
                     /label="SV40 ori"  
     misc_feature    6682..7159 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3363..3369 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3363 
                     /label="Spg7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGCC TTGCTGCTGC TTCGCGGGCT CCGCCCCGGC CCGGAGCCCC 
     1081 GGCCCCGACG GCTGTGGGGC CTGCTCTCAG GCAGGGGCCC AGGCCTCTCG TCCGGGGCTG 
     1141 GGGCCAGGCG GCCGTACGCG GCCCGCGGGA CTCCGGTTGG CCCGGCTGCG GCTGGAGGCC 
     1201 ACGCGCCTCA GAGTTTGCTA TTGCGAATAC TGACCCCTAG CTTTGAAGGT ATTAGCGGAT 
     1261 TGTTACTGAA ACAGCATATA GTTCCAAATG CAGTCAGACT GTGGCCGCTT TCAGGTAGCA 
     1321 CTTTGTATTT TAACACCTCA AGGATGAAGC AGAAGAATAA AGACAATGAT AAACCCAAAG 
     1381 GCAAGACCCC TGAAGATGAT GAAGAAGAGA AGAGACGAAA AGAGCGGGAA GACCAGATGT 
     1441 ACCGAGAGAG GCTGCGCACC CTGTTCATCA TTGCTCTTGT CATGAGCCTG CTGAACTCCC 
     1501 TCAGTACAAG TGGGGGCAGT ATTTCCTGGG CTGACTTTGT CAACGAGATG CTGGCTAAAG 
     1561 GCGAGGTGCA GCGTGTGCAG GTGGTGCCCG AGAGTGATGT GGTGGAAGTC TATCTGCATC 
     1621 CTGGAGCTGT GGTGTTTGGG CGGCCTCGGC TGGCCCTGAT GTATCGGATG CAGGTTGCAA 
     1681 ACATCGACAA ATTTGAAGAG AAGCTTCGAG CAGCCGAAGA TGAACTGAAC ATTGAGAGCA 
     1741 AGGACAGGAT TCCCGTGTCC TACAAGCGGA CAGGATTCTT TGGGAATGCC CTCTACGCCC 
     1801 TGGGGATGAC AGCCGTGGGC TTGGCCATCC TGTGGTATGT TTTCAGACTG GCGGGGATGA 
     1861 CCGGAAGGGA AGGCGGATTC AGTGCTTTTA ATCAGCTTAA GATGGCACGT TTCACCATTG 
     1921 TGGACGGGAA GACAGGGAAA GGAGTCAGCT TCCAAGATGT GGCAGGAATG CATGAAGCCA 
     1981 AGCTGGAAGT CCGAGAATTT GTGGATTATC TGAAGAGCCC AGAGCGTTTC CTTCAGCTCG 
     2041 GTGCCAAGGT TCCAAAGGGT GCCCTGTTGC TGGGGCCCCC TGGCTGTGGG AAGACGCTGT 
     2101 TGGCCAAGGC AGTAGCCACG GAGGCTCAGG TGCCCTTTTT AGCAATGGCT GGCCCAGAGT 
     2161 TTGTGGAGGT GATTGGAGGC CTGGGAGCTG CCCGAGTGCG AAGCCTCTTC AAGGAGGCAC 
     2221 GAGCCAGGGC CCCTTGCATA GTGTACATTG ATGAGATCGA TGCTGTGGGA AAGAAGCGCT 
     2281 CCACCTCCAT GTCTGGGTTC TCCAACACGG AAGAGGAGCA GACCCTCAAC CAGCTCCTGG 
     2341 TGGAGATGGA CGGAATGGGC ACCACAGACC ATGTCATCGT CTTGGCATCC ACCAATCGAG 
     2401 CTGATGTTCT GGACAATGCT CTGATGAGGC CTGGGCGGCT CGACAGGCAT GTCTTCATTG 
     2461 ATCTTCCCAC GCTCCAGGAG AGGCGGGAGA TTTTCGAGCA GCACCTGAAA GGCCTGAAGT 
     2521 TGACCCAGCC CAGCAGTTTT TACTCCCAGC GGCTGGCAGA GCTGACACCT GGATTCAGTG 
     2581 GCGCCGACAT CGCCAACATC TGCAACGAGG CCGCTCTGCA CGCTGCACGC GAGGGGCACA 
     2641 CGTCCGTGCA CACGTTCAAC TTTGAGTATG CCGTGGAGCG CGTCATTGCT GGTACTGCTA 
     2701 AAAAGAGTAA GATCCTCTCC AAGGAGGAGC AGAGAGTGGT CGCCTTCCAT GAGTCTGGCC 
     2761 ATGCCTTGGT TGGTTGGCTG CTGGAGCACA CAGAGGCTGT GATGAAGGTC TCCATCGCAC 
     2821 CTCGGACAAA CGCTGCTTTG GGCTTCTCTC AGATGCTCCC TCGGGACCAG TACCTCTTCA 
     2881 CCAAGGAGCA GCTGTTTGAG CGCATGTGCA TGGCCCTAGG TGGCCGTGCA GCTGAAGCCA 
     2941 TCTCATTCAG CAGGGTCACC TCTGGGGCCC AGGATGATTT GAGGAAGGTT ACCCGCATTG 
     3001 CTTACTCCAT GGTGAAACAG TTCGGGATGG CTCCCAGCAT TGGGCCTGTA TCCTTCCCCG 
     3061 AGGCACAAGA GGGCCTCATG GGCATTGGAC GTCGTCCCTT CAGCCAGGGC CTCCAGCAGA 
     3121 TGATGGACCA TGAAGCAAAG CTGCTGGTGG CCAAAGCCTA CAGACACACC GAGAAGGTGC 
     3181 TGCTAGACAA CCTGGACAAG CTGCAGGCGT TGGCGAATGC CCTTCTGGAA AAGGAAGTGA 
     3241 TAAACTATGA GGACATTGAG GCGCTCATTG GCCCGCCGCC CCATGGGCCA AAGAAAATGA 
     3301 TTGCACCACA GAAATGGATT GATGCTGAAA AGGAGAGACA GGCCTCAGGG GAGGAGGAGG 
     3361 CTCCGGCTCC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3421 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3481 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3541 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3601 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3661 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3721 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3781 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3841 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3901 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3961 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4021 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4081 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4141 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4201 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4261 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4861 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4921 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4981 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5041 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5101 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5161 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5221 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5281 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5341 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5401 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5461 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5521 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5581 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5641 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5701 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5761 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5821 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5881 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5941 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6001 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6061 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6121 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6181 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6241 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6301 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6361 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6421 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6481 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6541 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6661 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6781 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6841 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6901 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6961 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7021 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7081 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7141 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7201 TAACAAAAAT TTAACGCGAA TTTT
//