LOCUS       MR219855                8535 bp    DNA     circular  SYN 
DEFINITION  Kcnt1 (Myc-DDK-tagged) - Mouse potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 2
ACCESSION   NM_001145403
VERSION     NM_001145403.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4692..4783 
                     /label="Myc-DDK"  
     misc_feature    4813..5398 
                     /label="PolyA signal"  
     misc_feature    4870..4889 
                     /label="Restriction Enzyme"  
     misc_feature    5674..6346 
                     /label="ColE1"  
     misc_feature    6776..7570 
                     /label="Kan/Neo"  
     misc_feature    7605..7907 
                     /label="SV40 ori"  
     misc_feature    7993..8470 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4674..4680 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4674 
                     /label="Kcnt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACGACCTG GACACGGAGG TGCTGCCCTT GCCGCCAAGG TACCGATTCC 
     1081 GTGACCTGCT TTTAGGAGAC CAGCCATTCC CGAACGACGA TAGGCTTCAT GTGGAAGATT 
     1141 TCAGCCTGGA CTCTTCCCTT TCTCAGGTCC AGGTGGAGTT CTATGTCAAT GAGAACACCT 
     1201 TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG AGGATCCGGC 
     1261 TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT GTCCTGCTTG 
     1321 ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC ACTTTCAATG 
     1381 GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA ATGGCTCTGT 
     1441 GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC ATCATTTACC 
     1501 TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC TTGGAGATGA 
     1561 TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC CTGTTCATCC 
     1621 CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT AATGACTTCC 
     1681 ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC CTGTTCTGCA 
     1741 CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG CGAGCAGGTG 
     1801 GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA ACTGTGGGCT 
     1861 TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC CTGATCTGTG 
     1921 TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG ATGGAGCGTC 
     1981 AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC GTAGTCCTGT 
     2041 GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC TATGCCCATC 
     2101 CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC GTCCAGGTGC 
     2161 GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG GGCTCTGCCC 
     2221 TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC TTTATCCTCA 
     2281 GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT CGAGCCTGGG 
     2341 CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG CCCGAAAACA 
     2401 AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG TACGCCATGC 
     2461 TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG GTGCACACGT 
     2521 CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC GGGAGGTGCT 
     2581 CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG GAGTATGAGG 
     2641 GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG TGCCTCATCG 
     2701 GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG CACATCCTGG 
     2761 CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA GCTTTCATCT 
     2821 TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA TATGAAGGGC 
     2881 CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC ATGGACCTGC 
     2941 AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA AAGCTGACTC 
     3001 TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT CTGGAGTTGG 
     3061 CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG GATGAGGTGA 
     3121 CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT CCCCCCAACT 
     3181 CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA GCCCCCTTCT 
     3241 GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC AAGGCCTATG 
     3301 GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG CTCTACAACT 
     3361 TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT ATCGTGTTGC 
     3421 TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC CCCATGGTCT 
     3481 ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC ATCATCTATG 
     3541 CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG GACTACATGG 
     3601 CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC AGTCTCAGCA 
     3661 TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT GCCAAGGACA 
     3721 GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC TCCAACCTGG 
     3781 CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC ATGTTGGATA 
     3841 CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG CTGCTCTTGG 
     3901 GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC GAGGACGACC 
     3961 TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC GCCGAGATCC 
     4021 CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC GTCAGAGCCC 
     4081 AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC AAGGGACCCT 
     4141 GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG GGCAGTGCTG 
     4201 ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC AAGCTGAGTC 
     4261 GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC ACCCAGCAGC 
     4321 GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC AAGAACCGAA 
     4381 TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA ACAGCCAGTG 
     4441 ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT CATCACCAGA 
     4501 ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA CCCAACGACA 
     4561 TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC CAGAGTCGGA 
     4621 AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT GAGACCCAGC 
     4681 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4741 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4801 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4861 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4921 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4981 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5041 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5101 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5161 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5221 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5281 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5341 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5401 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5461 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5521 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5581 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5641 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5701 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5761 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5821 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5881 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5941 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6001 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6061 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6121 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6181 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6241 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6301 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6361 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6421 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6481 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6541 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6601 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6661 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6721 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6781 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6841 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6901 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6961 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7021 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7081 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7141 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7201 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7261 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7321 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7381 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7441 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7501 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7561 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7621 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7681 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7741 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7861 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7921 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7981 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8041 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8101 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8161 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8221 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8281 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8341 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8401 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8461 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8521 TTTAACGCGA ATTTT
//