LOCUS       MR219816                8976 bp    DNA     circular  SYN 
DEFINITION  Whsc1 (Myc-DDK-tagged) - Mouse Wolf-Hirschhorn syndrome candidate 1 (human) (Whsc1), transcript variant 2
ACCESSION   NM_175231
VERSION     NM_175231.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5133..5224 
                     /label="Myc-DDK"  
     misc_feature    5254..5839 
                     /label="PolyA signal"  
     misc_feature    5311..5330 
                     /label="Restriction Enzyme"  
     misc_feature    6115..6787 
                     /label="ColE1"  
     misc_feature    7217..8011 
                     /label="Kan/Neo"  
     misc_feature    8046..8348 
                     /label="SV40 ori"  
     misc_feature    8434..8911 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5115..5121 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5115 
                     /label="Nsd2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATTTAGC ATCAGAAAAA GTCCTCTTTC TGTTCAGAAA GTTGTAAAGT 
     1081 GCATGAAGAT GAAGCAGACA CCAGAAATCC TTGGCAGTGC AAATGGAAAG ACTCAGAACT 
     1141 GTGAAGTGAA TCATGAATGT TCTGTATTCC TCAGCAAAGC TCAACTTTCT AACAGCCTAC 
     1201 AGGAGGGGGT CATGCAGAAA TTTAATGGCC ATGATGCGCT CCCCTTTCTT CCAGCAGAGA 
     1261 AGTTGAAAGA TCTTACTTCT TGTGTTTTTA ATGGAGAACC TGGTGCTCAT GATACTAAAT 
     1321 TGTGTTTTGA GGCCCAGGAA GTAAAAGGAA TTGGGACACC ACCCAATACT ACACCTATCA 
     1381 AAAATGGCTC TCCAGAAATT AAACTGAAAA TCACCAAAAC ATACATGAAT GGGAAACCTC 
     1441 TCTTTGAATC TTCAATTTGT GGTGACGGTG CTGCTGATGT GTCTCAGTCA GAAGAAAACG 
     1501 AACAAAAATC TGATAATAAG ACTAGGAGGA ACAGGAAGAG GAGCATAAAA TATGACTCTT 
     1561 TACTGGAGCA GGGCCTTGTG GAAGCAGCTT TAGTGTCCAA GATCTCAAGT CCTGCAGATA 
     1621 AAAAGATTCC AGTTAAGAAG GAGTCCTGTC CAAATACTGG CAGAGACAGA GACCTCTTGT 
     1681 TAAAATACAA CGTTGGTGAT TTGGTGTGGT CCAAAGTGTC AGGTTACCCT TGGTGGCCTT 
     1741 GCATGGTTTC TGCTGACCCA CTCCTTCACA ATCATACCAA ACTTAAAGGT CAGAAAAAAA 
     1801 GTGCACGCCA GTATCATGTA CAGTTCTTTG GTGATGCCCC AGAAAGAGCT TGGATATTTG 
     1861 AGAAGAGCCT TGTTGCTTTT GAAGGAGAAG AACAATTTGA AAAATTATGC CAAGAAAGTG 
     1921 CCAAGCAGGC ACCCACGAAA GCTGAGAAAA TCAAGTTGTT GAAACCTATT TCGGGGAGAT 
     1981 TGAGAGCCCA GTGGGAAATG GGCATTGTCC AAGCTGAGGA AGCTGCCAGC ATGTCAATAG 
     2041 AGGAGCGGAA AGCCAAATTT ACCTTCCTTT ATGTGGGGGA CCAATTGCAC CTCAACCCTC 
     2101 AAGTAGCTAA GGAGGCTGGC ATTGTTACAG AACCTTTGGG AGAAATGGTG GACTCTTCAG 
     2161 GGGCCAGTGA AGAGGCTGCT GTAGACCCTG GGTCTGTGAG AGAAGAGGAT ATTCCCACGA 
     2221 AGAGAAGGCG AAGAACCAAA AGGTCTAGTT CTGCTGAGAA CCAAGAAGGT GATCCTGGCA 
     2281 CAGACAAGAG TACACCTCCA AAGATGGCAG AGGCTGAACC TAAGCGAGGA GTAGGCTCTC 
     2341 CTGCTGGGAG GAAAAAGTCC ACAGGCTCTG CTCCTCGGAG CAGGAAGGGA GACTCAGCAG 
     2401 CCCAGTTTTT AGTCTTTTGT CAAAAACACA GAGATGAGGT TGTAGCTGAA CATCCAGATG 
     2461 CCTCAGGGGA AGAGATTGAA GAATTGCTTG GGTCCCAGTG GAGCATGCTC AATGAAAAGC 
     2521 AGAAAGCACG ATATAATACA AAGTTTTCCC TAATGATCTC TGCCCAGTCT GAAGAAGACT 
     2581 CTGGAAATGG GAATGGGAAA AAAAGAAGCC ACACAAAGAG AGCAGATGAC CCTGCAGAGG 
     2641 ATGTGGATGT TGAAGACGCG CCCAGGAAAA GACTTAGAGC AGATAAGCAC AGTCTTCGGA 
     2701 AGAGAGAGAC AATCACCGAC AAAACGGCCA GAACAAGCTC TTACAAGGCT ATAGAGGCAG 
     2761 CCTCCTCACT CAAGAGCCAG GCAGCAACGA AAAATTTGTC TGATGCTTGC AAACCACTGA 
     2821 AGAAGCGAAA TCGGGCTTCT GCAACAGCGT CTTCAGCACT TGGGTTTAAC AAAAGTTCAT 
     2881 CTCCTTCTGC ATCCTTAACT GAGCATGAGG TCTCAGACAG CCCAGGAGAT GAACCATCAG 
     2941 AGTCTCCATA TGAAAGTGCA GATGAAACAC AGACTGAAGC ATCCGTCTCA TCTAAAAAAT 
     3001 CTGAAAGAGG AATGGCTGCC AAAAAGGAGT ACGTGTGTCA GCTGTGTGAG AAGACAGGCA 
     3061 GTCTCTTACT GTGTGAGGGC CCCTGTTGTG GAGCATTCCA CCTAGCCTGC CTTGGACTTT 
     3121 CCCGAAGACC GGAAGGAAGA TTCACCTGCA CCGAATGTGC CTCAGGGATT CACTCATGCT 
     3181 TCGTATGTAA AGAAAGCAAG ATGGAGGTGA AACGTTGTGT GGTAAATCAG TGTGGAAAGT 
     3241 TTTACCATGA AGCCTGTGTA AAAAAATACC CTCTCACTGT GTTTGAGAGC CGAGGCTTCC 
     3301 GCTGTCCTCT CCATAGCTGT ATGAGCTGCC ATGCCTCCAA CCCTTCAAAT CCACGGCCAT 
     3361 CAAAAGGTAA AATGATGCGA TGTGTCCGAT GCCCCGTTGC CTATCATGGA GGGGATGCCT 
     3421 GTCTGGCAGC AGGATGTTCG GTGATTGCTT CTAACAGCAT AATCTGCACA GGCCACTTCA 
     3481 CTGCCCGGAA GGGGAAGCGG CACCATACCC ATGTCAATGT GAGCTGGTGC TTTGTGTGCT 
     3541 CCAAAGGGGG AAGCCTTCTG TGCTGTGAGG CCTGCCCAGC AGCCTTTCAC CCCGATTGTC 
     3601 TAAACATCGA GATGCCCGAT GGTAGCTGGT TCTGCAACGA TTGCCGGGCT GGAAAGAAGC 
     3661 TCCACTTTCA AGACATCATC TGGGTCAAAC TTGGTAACTA CAGATGGTGG CCGGCAGAAG 
     3721 TTTGCCATCC CAAAAATGTT CCCCCAAATA TTCAGAAAAT GAAGCACGAG ATTGGAGAAT 
     3781 TCCCTGTATT TTTCTTTGGG TCTAAAGATT ATTACTGGAC GCATCAGGCA CGAGTGTTCC 
     3841 CATACATGGA GGGGGACCGG GGCAGCCGCT ACCAGGGGGT CAGAGGGATC GGAAGAGTCT 
     3901 TCAAGAACGC ATTGCAAGAA GCTGAAGCTC GTTTTAATGA AGTCAAACTT CAGCGGGAAG 
     3961 CCCGAGAAAC ACAGGAGAGT GAGAGAAAGC CTCCACCATA CAAGCATATC AAGGTGAATA 
     4021 AACCTTATGG TAAAGTCCAG ATCTACACAG CTGACATTTC TGAAATCCCG AAGTGCAACT 
     4081 GTAAGCCCAC AGATGAGAAC CCCTGTGGCT CTGATTCAGA GTGTCTGAAC AGGATGCTAA 
     4141 TGTTTGAGTG CCACCCGCAG GTGTGTCCTG CAGGGGAATA CTGCCAGAAC CAGTGTTTCA 
     4201 CTAAGCGCCA GTACCCTGAG ACCAAGATCA TCAAGACAGA TGGCAAAGGG TGGGGCCTGG 
     4261 TTGCCAAAAG GGACATCAGA AAAGGAGAAT TTGTTAATGA GTATGTTGGT GAGCTGATTG 
     4321 ATGAAGAGGA GTGTATGGCA AGAATCAAAT ATGCACATGA GAATGACATC ACTCACTTCT 
     4381 ACATGCTCAC CATAGACAAG GACCGCATAA TTGATGCTGG CCCCAAAGGG AATTATTCAC 
     4441 GATTCATGAA CCATAGCTGC CAGCCCAATT GTGAGACCCT GAAGTGGACA GTGAATGGGG 
     4501 ACACACGGGT TGGCCTGTTT GCTGTGTGTG ACATTCCTGC AGGTACAGAG CTGACTTTCA 
     4561 ACTATAACCT TGATTGTTTG GGCAATGAAA AGACAGTCTG TCGGTGTGGA GCCTCCAACT 
     4621 GTAGTGGGTT CCTTGGAGAC AGACCAAAGA CATCAGCATC CCTTTCATCA GAGGAAAAGG 
     4681 GTAAAAAGGC CAAGAAGAAA ACCAGAAGGC GCAGAGCCAA GGGTGAAGGA AAACGGCAGT 
     4741 CAGAGGATGA GTGCTTCCGC TGTGGTGATG GTGGGCAGCT GGTGCTGTGT GACCGCAAGT 
     4801 TCTGTACCAA GGCCTACCAC CTATCCTGCC TCGGTTTGGG CAAGCGGCCC TTTGGGAAGT 
     4861 GGGAATGTCC TTGGCATCAC TGTGATGTAT GTGGCAAACC TTCTACCTCA TTTTGCCACC 
     4921 TCTGCCCCAA CTCATTCTGT AAGGAACACC AAGATGGGAC TGCTTTCCGT TCCACCCAGG 
     4981 ATGGGCAGTC CTACTGCTGT GAGCATGACT TGAGGGCAGA CTCTTCAAGT AGCACCAAGA 
     5041 CTGAGAAACC CTTTCCAGAA TCACTGAAGT CAAAAGGAAA GAGGAAGAAA AGGCGGTGCT 
     5101 GGCGAAGGGT CACAGATGGC AAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5161 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5221 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5281 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5341 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5401 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5461 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5521 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5581 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5641 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5701 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5761 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5821 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5881 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5941 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6001 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6061 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6121 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6181 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6241 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6301 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6361 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6421 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6481 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6541 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6601 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6661 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6721 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6781 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6841 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6901 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6961 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7021 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7081 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7141 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7201 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7261 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7321 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7381 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7441 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7501 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7561 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7621 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7681 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7741 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7801 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7861 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7921 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7981 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8041 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8101 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8161 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8221 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8281 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8341 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8401 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8461 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8521 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8581 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8641 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8701 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8761 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8821 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8881 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8941 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//