LOCUS       MR219708                8058 bp    DNA     circular  SYN 
DEFINITION  Rps6kc1 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase polypeptide 1 (Rps6kc1)
ACCESSION   NM_178775
VERSION     NM_178775.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4213..4304 
                     /label="Myc-DDK"  
     misc_feature    4334..4919 
                     /label="PolyA signal"  
     misc_feature    4391..4410 
                     /label="Restriction Enzyme"  
     misc_feature    5195..5867 
                     /label="ColE1"  
     misc_feature    6297..7091 
                     /label="Kan/Neo"  
     misc_feature    7126..7428 
                     /label="SV40 ori"  
     misc_feature    7514..7991 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4188..4195 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4188 
                     /label="Rps6kc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTCTCCT TGGGGTCACA GTGCGGACCT GGCCCGCTTC TACACTGTCA 
     1081 CCGAGCCCCA GCGACACCCC AGGGGCTACA CTGTCTATAA GGTTACCGCA CGGGTTGTTT 
     1141 CACGGAGAAA TCCAGAGGAC GTCCAGGAGA TAATTGTGTG GAGGAGATAC AGTGACTTCA 
     1201 AGAAACTGCA CAGAGAATTA TGGCAAATTC ACAGGAATGC ATTTCGACAC TCTGAGCTAT 
     1261 TTCCCCCGTT TGCTAAAGGG ACAGTGTTTG GGCGGTTCGA CAAGACGGTG ATTGAGGAGC 
     1321 GACGGCAGTG TGCTGAGGAC CTGCTGCAGT TCTCTGCCAA CATCCCCGCC CTGTACAACA 
     1381 GCAGGCAGCT CCAGGACTTC TTCAAGGGTG GGGTAATTAG TGATGGCTCT GAGTTAATTG 
     1441 GCCCTGCTGA AGCTTACCCA GACTCCCCGG CCAACGCCTT TCCTGAGTGC GGTACTGAAG 
     1501 GATTCTCCAG TGACAGTGAC CTTCTGTCCC TCACTGTTGA TGCAGACTCT CTTGCCGAGG 
     1561 TAGATGATGG AATGGCTTCC CGTCAGGGTT CTCCCAGTAG AACCTTTGGT CTGAGTCTGT 
     1621 CTACGGATTC TTCGGCAGTA GGGGCTGTTG CTTCTGACAG TGAACCAAGC AGAGTAGAAG 
     1681 ACCGGGAAAG CCGCAGCCTC TTCCCCAGCA GCTTAAAGCC AAGGCTTGGC AGGAGAGATT 
     1741 ACCTGGAGAA GGCAGGGGAA CTTATCAAAC TGGCCTTAAA GAAGGAGGAG GAAGATGACT 
     1801 ATGAAGCTGC TTCTGACTTT TACAGGAAGG GCGTCGATCT GCTCCTAGAA GGTGTACAAG 
     1861 GAGAGTCGAG CCCCACGCGT CGGGAGGCTG TGAAGAGAAG GACTGCGGAG TACCTCATGC 
     1921 GTGCAGAGAG CATCTGTAGC CTCCGGGCCG CGCCTCAGCT CCACACGGGA CCACAGCCTC 
     1981 CAGGATCACT AAGTTCAAGG CCCCCTTGGA GCCTGAGGAG CCCTGCCGAG GAGCTGAAGG 
     2041 CCTTCCGAGT CCTTGGGGTG ATTGACAAGG TTTTACTTGT AATGGATACA AGGACAGAGC 
     2101 AGACATTCAT TTTAAAAGGT CTAAGGAAAA GCAGCGAGTG CAGCAGGAAC AGAAAGACCA 
     2161 TCATCCCCCG CTGTGTGCCT AACATGGTGT GTCTGCACAC GTACATCATC TCGGAGGAAT 
     2221 CGGTGTTTCT CGTGCTGCAG CGCGCCGAAG GTGGAAAACT CTGGTCGTAT ATCAGTAAAT 
     2281 TTCTGAACAG AAGCTCTCAG GAAAGCTTGG ACATTAAGGA AGGGAGACCG TCCATGCCTC 
     2341 CTCGAGTGTG CCTGCAGCAG CCAAGTGCCA GTCCCCAGGG CGGCAGCAGC TTCGAGTCCA 
     2401 GAGGGAGCGA CACGGGGAGC ATGCTGAAGG CTCTGCCTCT GAAGACCAGT CTCACCCCGA 
     2461 GTTCTCAAGA CGACAGCAAC CAGGAAGACG ACGGCCAGCC CAGCTCTCCC AAGTGGCTGG 
     2521 ATTCGGGCTC CAGTTCAGAG GATGAGTGCA CAGCGGGCTA CCTGACGCTG TGCAACGAGT 
     2581 ATGGGCAAGA AAAGATGGAC CTGGTGTCTC TGAGCGAGGA GTCTGTTATG CAGCCTGAAG 
     2641 GGGACAAGGC TGACACCCAG GCGGTTAGTT CCCCAGCATC CCTCGCCACT GGCAGTGTGA 
     2701 GCCCTAGCAC CCACCTCAGG GTCTTCTCTG GAGGTGAGGA TCTAGAGGCG GTTTCTTCTC 
     2761 CTCCAACATC TGAATCCCTC AGTAGGTCAA AAAACAGCCC TATGGAATTC TTCAGAATAG 
     2821 ACAGTAAAGA CAGCACAAGT GAGCTCCTGG GACTGGATTT TGGTGAGAAA TTACACAGTC 
     2881 TGAAACCAGA GCCTTTGAAA GCCCTCTTTA CCCTTGAAGA TGGAGACAGC CCTTCTCAGA 
     2941 GTCTTGATCC TGGTGAGAGC AAGAGAGAGA GTGAAGCTCA GGACTCCGTG AGCCGGGGCT 
     3001 CAGATGATTC CGTCCCAGTT ATCTCATTTA AAGAGGCGGC TGCAGAGGCT ATCAGTGGGG 
     3061 CTGAGGAGGG AAGGCCTGAC CTTCTTGTGA ATTTACCTGG GGAACTGCAG CCAACCAAAG 
     3121 AGGCTTCAGC AATGGACCCT AAGTTTTCAC AGGCCAGTGC AGGCAGGCTG GATAGTAAAC 
     3181 TGCTGGAAGC CCCGGATGTG TTGTGCCTCA GGCTGAGTTC TGAACAGTGC CATGGTCTGG 
     3241 GACCAGAAGG CCCAGAGGAA CTGAGCGATC CCACTGAATT CTGTCCCGGA GGTGTGATCC 
     3301 CAGAGCACGA TGCTCAGGCA GATCCCGGGG TGCTATTCGA GGCTGCCGTT GATCACAGGA 
     3361 GCTCCCCAGA CCAGTTTCTG TTTTCCAGCT TGCGTTCCGA GTCTGACAGG CTGGGGCAGG 
     3421 TGGAGGTAGT GGTCACTGCA CAGGCCTTGC AAGAAAGCCT GTTCCACATT TCTAGTCCCT 
     3481 GCTCAGGTGC TAATAAAGAA CACAGTGCAT ACGCCGACAC TGCCACGTCG GAGGAGGTGT 
     3541 TGCTGTTCAC AGAACCGACT AAAGAAGAAG CGAACTCTCT GTTCCAGAGA GACTCGGAGG 
     3601 CCCAGGAGCG AGGTGTGGGA GCCGGGGAAG CAGACAAGGA AATCCATCAG ATCTTTGAGG 
     3661 ACCTGGATAA ACGACTAGCA GCTAGCTCCA GGTTCTTCAT CCCAGAGGGC TGCATTCAGA 
     3721 GATGGGCAGC GGAAATGGTG GTAGCCCTTG ACGCCTTGCA CAGAGAGGGG ATTGTGTGCC 
     3781 GAGATCTGAA CCCAAACAAC ATCTTATTGA ATGATGGAGG ACACATTCAG CTAACGTATT 
     3841 TTAGCAGGTG GAGTGAGGTT GAAGATTCCT GCGACAGCGA TGCCGTAGCG AGAATGTACT 
     3901 GTGCCCCAGA GGTTGGAGCA GTCACAGAAG AAACTGAAGC CTGTGATTGG TGGAGCCTGG 
     3961 GTGCTGTCCT CTTCGAACTT CTCACTGGCA AGACGTTGGT GGAATGCCAC CCAGCCGGAA 
     4021 TAAATACCCA CACTACTTTG AACATGCCAG GTTGTGTTTC TGAAGAGGCT CGCTCACTCA 
     4081 TCCAACAGCT CCTGCAGTTC AACCCTATGG AGCGCCTGGG TGCTGGAGTG GCTGGTGTTG 
     4141 AAGATATCAA ATCTCACCCG TTTTTCACCC CTGTGGATTG GGCAGAGTTG ACAAGAAGCG 
     4201 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4261 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4321 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4381 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4441 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4501 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4561 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4621 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4681 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4741 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4801 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4861 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4921 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4981 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5041 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5101 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5161 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5221 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5281 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5341 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5401 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5461 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5521 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5581 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5641 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5701 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5761 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5821 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5881 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5941 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6001 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6061 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6121 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6181 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6241 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6301 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6361 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6421 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6481 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6541 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6601 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6661 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6721 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6781 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6841 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6901 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6961 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7021 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7081 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7141 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7201 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7261 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7321 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7381 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7501 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7561 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7621 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7681 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7741 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7801 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7861 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7921 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7981 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8041 AAATTTAACG CGAATTTT
//