LOCUS       MR219575                7908 bp    DNA     circular  SYN 
DEFINITION  Usp35 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 35 (Usp35). Note: ORF is codon optimized
ACCESSION   NM_001177412
VERSION     NM_001177412.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4065..4156 
                     /label="Myc-DDK"  
     misc_feature    4186..4771 
                     /label="PolyA signal"  
     misc_feature    4243..4262 
                     /label="Restriction Enzyme"  
     misc_feature    5047..5719 
                     /label="ColE1"  
     misc_feature    6149..6943 
                     /label="Kan/Neo"  
     misc_feature    6978..7280 
                     /label="SV40 ori"  
     misc_feature    7366..7843 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4047..4053 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4047 
                     /label="Usp35"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATAAAATA CTGGAAGCAG TGGTGACTAG CAGTTACCCT GCCAGTGTGA 
     1081 AGCAGGGACT GGTGAGAAGA GTGCTGGAGG CCGCCAGACA GCCCCTTGAG CGCGAACAGT 
     1141 GTTTGGCACT CCTCGCTCTG GGAGCTAGGC TGTACGTGGG GGGTACCGAA GAACTCCCCA 
     1201 GGAGAGTGGG CTGCCAGCTG CTCCATGTTG CCGGCCGGCA TCATCCCGAC GTGTTTGCCG 
     1261 AGTTCTTCAG CGCTAGGCGC GTCCTGCGCC TTCTTCAGGG GGGCGCTGGA CCTCCTGGTG 
     1321 CGAGAGCACT GGCCTGCGTT CAGCTCGGGT TGCAACTCTT GCCTGATGGC CCTGCCGCGG 
     1381 ACGAGGTCTT CGCCCTGCTC CGCAGGGAGG TACTGAGAAC TGTTTGTGAA CGGCCGGGCC 
     1441 CCGCTGCTTG TGCTCAGGTG GCCCGCCTGT TGGCTAGGCA CCCACGCTGT GTGCCAGACG 
     1501 GTGCCCACAG GCTGCTCTTC TGCCAACAGC TTGTGAGATG CCTGGGCAGA TTTAGGTGTC 
     1561 CTGCCGAAGG TGAGGAGGGC GCAGTAGAGT TCCTGGAGCA GGCCCAACAG GTGTCCGGGC 
     1621 TGCTCGCGCA GTTGTGGAGA GCACAGCCCG CCGCCATCCT CCCTTGTCTG AAAGAGCTTT 
     1681 TTGCCGTGAT CTCCTGTACC GAGGAAGAGC CACCCTCTAG TGCCCTCGCC TCAGTGGTAC 
     1741 AGCATCTTCC CCTTGAATTG ATGGATGGTG TGGTCCGGAA CCTCAGCAAT GATGATAGTG 
     1801 TGACAGACTC CCAGATGCTG ACTGCCATTA GCAGGATGAT CGACTGGGTG TCCTGGCCCC 
     1861 TGGGGAAGAA CATTGACAAG TGGATCATTG CCCTGCTGAA GGGCCTGGCT GCTGTTAAGA 
     1921 AGTTCAGCAT CTTGATCGAG GTTTCGCTCG CCAAAATTGA GAAGGTCTTC TCTAAGCTGC 
     1981 TGTACCCCAT CGTGCGGGGA GCTGCCCTAT CTGTCCTTAA GTACATGCTC CTGACTTTCC 
     2041 AGCACTCCCA TGAGGCCTTC CACCTGCTTC TCCCTCACAT TCCCGCCATG GTGGCCTCTC 
     2101 TGGTCAAGGA GGACTCGAAC TCTGGGACCA GCTGCCTGGA GCAGCTGGCA GAGCTGGTGC 
     2161 ACTGCATGGT GTTTCGGTTC CCGGGCTTCC CAGACCTGTA TGAGCCTGTC ATGGAAGCCA 
     2221 TCAAGGACCT CCACATTCCC AATGAGGACC GAATCAAGCA GCTGCTGGGG CAGGATGCCT 
     2281 GGACCTCACA GAAGAGTGAA CTGGCTGGCT TCTATCCCCG GCTCATGGCC AAGTCAGACA 
     2341 CGGGCAAGAT TGGCTTAATT AACTTGGGCA ACACATGCTA CGTGAACAGT GTCCTTCAGG 
     2401 CCTTATTCAT GGCTTCTGAC TTTCGACACT GTGTGCTCCG CTTGACTGAG AACAACTCCC 
     2461 AGCCCTTGAT GACCAAGCTG CAGTGGCTCT TTGCCTTCCT GGAGCACAGT CAGAGGCCTG 
     2521 CCATCTCTCC TGAGAACTTC CTCTCTGCGT CCTGGACGCC CTGGTTCAGC CCTGGCACCC 
     2581 AGCAAGACTG TTCAGAGTAT CTGAAGTACC TGCTGGATCG GCTTCATGAA GAAGAGAAAA 
     2641 CTGGGATGAG GATCTGCCAG AAGCTCAAGC AGTCCAGCTT GCCATCCCCA CAGGAAGAGC 
     2701 TCCCCAGCTC CAATGCAACG TCGGTGGAGA AGATGTTCGG AGGCAAGATT GTGACCCGGA 
     2761 TATGCTGTCT CCACTGCCTC AATGTTTCCT CAAGGGAGGA AGCCTTCACA GACCTCTCTT 
     2821 TGGCCTTCCC TCCTCCTGAG AGAAGTCGCC ACCGCCGCCT CGGCTCCGTG ATGCTCCCCA 
     2881 CGGAGGATGT GCGAGTCCAG GAGTTGACAC TGGCTCCCAG AGCCCCCGGG GCGCAGAGGC 
     2941 AGAGGAAGCG CTGCATCACA GGGGACGCTC CTCGCACTGG GCTGGACAGT GAAGGTGTGG 
     3001 ACACCATAGG CACTGGTGGA CGGAGTGGGC AGGAGAAGGT GGAGAGGGAG CAGGCTGGGA 
     3061 AGGAGAAGGA GGTGGCAGAG GACAGGGAAG AGGAAGGAAC AAGGGAAGAG GAGAAAGAAG 
     3121 AGGGGGAAGA GAAAGACAAA GAGAAAGAGA AGAAGGAAGA TGAAAAGGAA AAGGAAGCTG 
     3181 AGAATGGCAA GGAGAAGGAA GGGGACAGCT TAGGACCAGG GACCCATAGG GAGGCTGCCA 
     3241 CTCCACCCAG GGAGCAGACA TGCGGCCCTG AGGGTTCCCG CTCTGTACTG GACTTGGTCA 
     3301 ACTACTTCCT GTCCCCTGAG AGGCTGACAG CTGAGAACCG CTACTACTGT GAGTCCTGTG 
     3361 CCTCCCTGCA GGATGCGGAG AAGGTGGTGG AGCTGAGCCA GGGTCCACGC TACCTCATCC 
     3421 TCACACTACT GCGCTTCTCC TTTGACCTGC GTACCATGCG GCGGCGTAAG ATCCTGGACG 
     3481 ATGTCACCAT CCCCCTTTTG CTGCGCCTGC CACTGGCTGG GGGTCAGGGC CAGGCCTATG 
     3541 ACCTCTGTAG TGTGGTGGTG CACTCTGGAG TGTCCTCAGA GAGTGGCCAC TATTACTGCT 
     3601 ATGCTCGTGA GGGTGCTGCT CGGCCAGCTC CTGTCCTGGG ATCCACAGAG AGGCCTGAGC 
     3661 CTGAGAACCA GTGGTACCTG TTTAATGATA CCCGGGTGTC TTACTCATCC TTTGAGTCTG 
     3721 TCAGCAATGT CACCTCCTTC TTCCCTAAGG ACACTGCCTA TGTGCTCTTC TACCGCCAGA 
     3781 GGCCCAGGGA GGACCCTGCG GCTGAGCCCG GCTCTCCTAG AGTCCGAGCA GAGCCTACCC 
     3841 TCCACAAGGA CCTGATGGAG GCCATTTCCA AAGACAATGT CCTCTACCTG CAGGAGCAGG 
     3901 AGAAGGAGGC CCGGAGCAGG GCAGCTTATA TCTCTACACT CCCTGCCCCT CCACACTGGG 
     3961 GTAGGGGCTT CGATGAAGAC AAAGATGAGG ATGAAGGCTC ACCAGGGGGC TGCAACCCTG 
     4021 CAGGTGGCAA TGGTGACTTC CACAGACTGG TCTTCACGCG TACGCGGCCG CTCGAGCAGA 
     4081 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4141 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4201 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4261 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4321 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4381 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4441 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4501 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4561 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4621 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4681 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4741 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4801 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4861 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4921 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4981 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5041 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5101 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5161 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5221 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5281 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5341 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5401 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5461 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5521 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5581 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5641 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5701 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5761 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5821 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5881 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5941 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6001 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6061 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6121 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6181 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6241 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6301 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6361 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6421 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6481 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6541 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6601 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6661 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6721 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6781 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6841 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6901 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6961 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7021 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7081 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7141 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7261 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7321 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7381 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7441 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7501 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7561 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7621 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7681 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7741 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7801 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7861 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//