LOCUS       MR219512                8439 bp    DNA     circular  SYN 
DEFINITION  Nrcam (Myc-DDK-tagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam), transcript variant 2
ACCESSION   NM_001146031
VERSION     NM_001146031.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4596..4687 
                     /label="Myc-DDK"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    4774..4793 
                     /label="Restriction Enzyme"  
     misc_feature    5578..6250 
                     /label="ColE1"  
     misc_feature    6680..7474 
                     /label="Kan/Neo"  
     misc_feature    7509..7811 
                     /label="SV40 ori"  
     misc_feature    7897..8374 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4578..4584 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4578 
                     /label="Nrcam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCTTAAA ATAATGCCGA AGAAGAAGCA CTTATCTGCA GGCGGAGTGC 
     1081 CCCTGATTCT CTTCCTGTGC CAGATGATCA GCGCGCTGGA TGTTCCTCTC GATCTGGTAC 
     1141 AACCTCCAAC CATCACTCAA CAGTCACCAA AAGACTACAT CATTGACCCT CGGGAGAATA 
     1201 TTGTAATCCA GTGTGAGGCC AAAGGGAAAC CTCCCCCAAG CTTTTCCTGG ACTCGTAATG 
     1261 GAACTCACTT TGACATAGAC AAGGACCCTC TGGTCACCAT GAAGCCTGGC TCAGGAACCC 
     1321 TTGTCATCAA CATCATGAGT GAGGGGAAGG CAGAGACCTA TGAAGGAGTC TACCAGTGCA 
     1381 CTGCAAGGAA CGAGCGTGGA GCTGCCGTCT CCAATAACAT TGTTGTCCGC CCATCTAGGT 
     1441 CACCCTTGTG GACCAAGGAA AGACTTGAAC CTATAGTACT CCAGAATGGG CAGTCATTAG 
     1501 TACTTCCATG TAGGCCTCCG ATTGGATTAC CTCCGGCCAT AATATTTTGG ATGGATAATT 
     1561 CCTTTCAGAG ACTTCCACAG AGTGAGCGGG TTTCCCAAGG CCTAAATGGA GACCTTTACT 
     1621 TCTCCAATGT CCTCCCAGAG GACACCCGTG AGGACTATAT CTGCTATGCC AGATTTAATC 
     1681 ACACTCAAAC CATCCAGCAG AAGCAGCCTA TTTCTTTGAA GGTGATTTCA GTGGATGAAT 
     1741 TGAATGACAC TATAGCTGCT AATTTGAGTG ACACTGAGTT TTATGGTGCT AAATCTAGTA 
     1801 AAGAGAGGCC CCCAACGTTT CTAACTCCAG AGGGCAATGA AAGCCACAAA GAGGAATTAA 
     1861 GAGGAAACGT GCTTTCGCTG GAGTGCATTG CGGAAGGCCT ACCTACTCCA ATTATTTACT 
     1921 GGATCAAAGA AGACGGAATG CTTCCCGCCA ACCGGACATT TTATCGGAAC TTTAAGAAAA 
     1981 CCCTGCAGAT CACTCATGTT TCCGAAGCCG ACTCTGGAAA TTATCAGTGC ATAGCAAAAA 
     2041 ACGCATTAGG AGCCGTCCAT CACACCATTT CTGTCACTGT TAAAGCGGCT CCCTACTGGA 
     2101 TCGTGGCACC TCAAAACCTC GTGCTTTCCC CGGGAGAGAA TGGGACCCTC ATCTGCAGAG 
     2161 CTAATGGCAA CCCGAAACCC AGAATTAGCT GGTTAACAAA TGGAGTCCCA ATAGAAATTG 
     2221 CTCTCGATGA CCCCAGCAGA AAAATAGATG GCGATACCAT TATATTTTCA AATGTTCAAG 
     2281 AAAGCTCAAG TGCGGTTTAT CAGTGCAATG CCTCTAACAA ATATGGATAT TTACTAGCAA 
     2341 ATGCATTTGT AAATGTTCTC GCTGAACCAC CTCGGATTCT CACATCAGCA AACACACTGT 
     2401 ACCAGGTCAT TGCAAACAGG CCTGCTTTGC TAGATTGTGC CTTCTTTGGA TCTCCTATGC 
     2461 CTACCATTGA GTGGTTTAAA GGCACTAAAG GAAGCGCTCT TCATGAAGAC ATTTATGTTT 
     2521 TGCATGATAA TGGAACATTA GAAATTCCCG TGGCCCAAAA GGATAGTACA GGGACGTATA 
     2581 CTTGTGTCGC ACGGAATAAA CTAGGGATGG CAAAGAATGA AGTTCACTTG GAAATCAAAG 
     2641 ATCCAACCAG GATCATTAAA CAACCTGAGT ATGCAGTCGT CCAGAGGGGG AGCAAGGTGT 
     2701 CCTTTGAATG CAGAGTGAAA CATGACCACA CCTTAATCCC CACCATTATG TGGCTGAAGG 
     2761 ACAATGGAGA GCTGCCCAAT GATGAAAGGT TCTCCACTGA CAAGGATCAT CTGGTGGTAT 
     2821 CTGATGTAAA GGATGACGAT GGCGGAACCT ACACGTGTAC GGCCAACACA ACGCTGGACA 
     2881 GTGCTTCGGC CAGCGCTGTG CTCAGGGTTG TCGATGTCCC GAATCCTCCC TTTGATTTAG 
     2941 AATTGACCAA TCAACTTGAC AAAAGTGTTC AGCTGACATG GACCCCAGGC GACGACAACA 
     3001 ATAGCCCCAT TACAAAATTC ATCATTGAGT ATGAAGATGC AATGCATGAT GCAGGGCTGT 
     3061 GGCGCCACCA GGCTGAAGTT TCTGGAACAC AGACCACAGC CCAACTGAAG CTGTCTCCCT 
     3121 ATGTGAACTA CTCCTTCCGT GTCATGGCAG AGAACAGCAT TGGGAGAAGT ATGCCGAGCG 
     3181 AGGCATCCGA GCAGTATCTT ACAAAAGCCG CAGAACCAGA TCAGAATCCC ATGGCTGTGG 
     3241 AAGGACTAGG GACAGAGCCG GACAACTTGG TGATTACATG GAAGCCCCTG AATGGTTTTC 
     3301 AATCGAATGG GCCTGGCCTC CAGTACAAAG TGAGCTGGCG CCAGAAAGAT GGTGACGATG 
     3361 AGTGGACGTC TGTGGTTGTG GCCAATGTAT CCAAATACAT TGTTTCTGGC ACACCAACCT 
     3421 TTGTCCCATA CCTGATAAAA GTTCAAGCTC TGAATGATGT GGGGTTTGCA CCAGAGCCAG 
     3481 CTGCAGTCAT GGGGCATTCT GGAGAAGACC TTCCAATGGT GGCTCCTGGA AATGTTCGCG 
     3541 TCAGCGTGGT GAACAGTACG CTGGCAGAGG TGCACTGGGA CCCAGTTCCT CCCAAGAGTG 
     3601 TCCGAGGACA CTTACAAGGC TACCGGATTT ACTACTGGAA GACCCAGAGC TCCTCTAAAA 
     3661 GAAACAGGCG CCACATTGAG AAGAAGATCC TCACCTTCCA GGGCACCAAG ACTCACGGCA 
     3721 TGCTGCCAGG GCTGCAGCCA TACAGTCACT ATGCCCTCAA CGTCCGAGTG GTCAACGGGA 
     3781 AAGGGGAGGG CCCAGCCAGC ACGGACAGAG GCTTCCATAC ACCGGAGGGA GTCCCTAGTG 
     3841 CTCCCTCATC TTTGAAAATT GTTAATCCCA CACTGGACTC CCTCACTTTG GAATGGGACC 
     3901 CCCCAAGCCA CCCAAATGGC ATCCTGACTG AGTACATCTT ACAATATCAG CCAATTAACA 
     3961 GCACACATGA GCTAGGCCCT CTGGTAGATT TAAAAATTCC TGCCAACAAG ACCCGCTGGA 
     4021 CTTTGAAAAA TTTAAATTTC AGCACTCGGT ACAAGTTCTA TTTCTATGCA CAGACATCAG 
     4081 TGGGGCCAGG CAGTCAGATC ACAGAGGAAG CGATAACGAC TGTGGACGAA GGTAAGAAGG 
     4141 CAATGGCAAG CCGGCAAGTG GATATCGCGA CCCAAGGCTG GTTCATAGGT CTAATGTGCG 
     4201 CTGTTGCTCT CCTCATCTTA ATTTTGCTGA TTGTTTGCTT CATCAGAAGA AACAAAGGTG 
     4261 GTAAATACCC AGTTAAAGAA AAGGAGGATG CTCATGCAGA CCCTGAAATC CAGCCCATGA 
     4321 AGGAAGATGA TGGGACGTTT GGAGAATACA GTGATGCAGA AGATCACAAG CCTTTGAAAA 
     4381 AAGGAAGTCG AACACCTTCA GACAGGACTG TGAAAAAAGA AGATAGCGAT GATAGTCTGG 
     4441 TTGACTATGG AGAGGGGGTG AACGGCCAAT TCAACGAGGA TGGCTCCTTT ATTGGCCAAT 
     4501 ACAGTGGTAA GAAAGAGAAA GAGCCAGCAG AGGGAAATGA AAGCTCAGAG GCCCCTTCTC 
     4561 CTGTCAACGC AATGAACTCC TTTGTTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4621 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5401 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5461 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5521 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5581 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5641 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5701 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5761 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5821 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5881 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5941 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6001 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6061 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6121 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6181 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6241 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6301 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6361 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6421 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6481 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6541 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6601 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6661 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6721 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6781 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6841 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6901 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6961 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7021 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7081 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7141 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7201 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7261 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7321 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7381 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7441 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7501 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7561 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7621 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7681 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7861 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7921 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7981 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8041 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8101 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8161 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8221 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8281 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8341 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8401 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//