LOCUS       MR219511                8649 bp    DNA     circular  SYN 
DEFINITION  Nrcam (Myc-DDK-tagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam), transcript variant 1
ACCESSION   NM_176930
VERSION     NM_176930.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="Nrcam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCTTAAA ATAATGCCGA AGAAGAAGCA CTTATCTGCA GGCGGAGTGC 
     1081 CCCTGATTCT CTTCCTGTGC CAGATGATCA GCGCGCTGGA TGTTCCTCTC GATCTGGTAC 
     1141 AACCTCCAAC CATCACTCAA CAGTCACCAA AAGACTACAT CATTGACCCT CGGGAGAATA 
     1201 TTGTAATCCA GTGTGAGGCC AAAGGGAAAC CTCCCCCAAG CTTTTCCTGG ACTCGTAATG 
     1261 GAACTCACTT TGACATAGAC AAGGACCCTC TGGTCACCAT GAAGCCTGGC TCAGGAACCC 
     1321 TTGTCATCAA CATCATGAGT GAGGGGAAGG CAGAGACCTA TGAAGGAGTC TACCAGTGCA 
     1381 CTGCAAGGAA CGAGCGTGGA GCTGCCGTCT CCAATAACAT TGTTGTCCGC CCATCTAGGT 
     1441 CACCCTTGTG GACCAAGGAA AGACTTGAAC CTATAGTACT CCAGAATGGG CAGTCATTAG 
     1501 TACTTCCATG TAGGCCTCCG ATTGGATTAC CTCCGGCCAT AATATTTTGG ATGGATAATT 
     1561 CCTTTCAGAG ACTTCCACAG AGTGAGCGGG TTTCCCAAGG CCTAAATGGA GACCTTTACT 
     1621 TCTCCAATGT CCTCCCAGAG GACACCCGTG AGGACTATAT CTGCTATGCC AGATTTAATC 
     1681 ACACTCAAAC CATCCAGCAG AAGCAGCCTA TTTCTTTGAA GGTGATTTCA GTGGATGAAT 
     1741 TGAATGACAC TATAGCTGCT AATTTGAGTG ACACTGAGTT TTATGGTGCT AAATCTAGTA 
     1801 AAGAGAGGCC CCCAACGTTT CTAACTCCAG AGGGCAATGA AAGCCACAAA GAGGAATTAA 
     1861 GAGGAAACGT GCTTTCGCTG GAGTGCATTG CGGAAGGCCT ACCTACTCCA ATTATTTACT 
     1921 GGATCAAAGA AGACGGAATG CTTCCCGCCA ACCGGACATT TTATCGGAAC TTTAAGAAAA 
     1981 CCCTGCAGAT CACTCATGTT TCCGAAGCCG ACTCTGGAAA TTATCAGTGC ATAGCAAAAA 
     2041 ACGCATTAGG AGCCGTCCAT CACACCATTT CTGTCACTGT TAAAGCGGCT CCCTACTGGA 
     2101 TCGTGGCACC TCAAAACCTC GTGCTTTCCC CGGGAGAGAA TGGGACCCTC ATCTGCAGAG 
     2161 CTAATGGCAA CCCGAAACCC AGAATTAGCT GGTTAACAAA TGGAGTCCCA ATAGAAATTG 
     2221 CTCTCGATGA CCCCAGCAGA AAAATAGATG GCGATACCAT TATATTTTCA AATGTTCAAG 
     2281 AAAGCTCAAG TGCGGTTTAT CAGTGCAATG CCTCTAACAA ATATGGATAT TTACTAGCAA 
     2341 ATGCATTTGT AAATGTTCTC GCTGAACCAC CTCGGATTCT CACATCAGCA AACACACTGT 
     2401 ACCAGGTCAT TGCAAACAGG CCTGCTTTGC TAGATTGTGC CTTCTTTGGA TCTCCTATGC 
     2461 CTACCATTGA GTGGTTTAAA GGCACTAAAG GAAGCGCTCT TCATGAAGAC ATTTATGTTT 
     2521 TGCATGATAA TGGAACATTA GAAATTCCCG TGGCCCAAAA GGATAGTACA GGGACGTATA 
     2581 CTTGTGTCGC ACGGAATAAA CTAGGGATGG CAAAGAATGA AGTTCACTTG GAAATCAAAG 
     2641 ATCCAACCAG GATCATTAAA CAACCTGAGT ATGCAGTCGT CCAGAGGGGG AGCAAGGTGT 
     2701 CCTTTGAATG CAGAGTGAAA CATGACCACA CCTTAATCCC CACCATTATG TGGCTGAAGG 
     2761 ACAATGGAGA GCTGCCCAAT GATGAAAGGT TCTCCACTGA CAAGGATCAT CTGGTGGTAT 
     2821 CTGATGTAAA GGATGACGAT GGCGGAACCT ACACGTGTAC GGCCAACACA ACGCTGGACA 
     2881 GTGCTTCGGC CAGCGCTGTG CTCAGGGTTG TCGCTCCTAC TCCAACTCCA GCCCCCATTT 
     2941 ACGATGTCCC GAATCCTCCC TTTGATTTAG AATTGACCAA TCAACTTGAC AAAAGTGTTC 
     3001 AGCTGACATG GACCCCAGGC GACGACAACA ATAGCCCCAT TACAAAATTC ATCATTGAGT 
     3061 ATGAAGATGC AATGCATGAT GCAGGGCTGT GGCGCCACCA GGCTGAAGTT TCTGGAACAC 
     3121 AGACCACAGC CCAACTGAAG CTGTCTCCCT ATGTGAACTA CTCCTTCCGT GTCATGGCAG 
     3181 AGAACAGCAT TGGGAGAAGT ATGCCGAGCG AGGCATCCGA GCAGTATCTT ACAAAAGCCG 
     3241 CAGAACCAGA TCAGAATCCC ATGGCTGTGG AAGGACTAGG GACAGAGCCG GACAACTTGG 
     3301 TGATTACATG GAAGCCCCTG AATGGTTTTC AATCGAATGG GCCTGGCCTC CAGTACAAAG 
     3361 TGAGCTGGCG CCAGAAAGAT GGTGACGATG AGTGGACGTC TGTGGTTGTG GCCAATGTAT 
     3421 CCAAATACAT TGTTTCTGGC ACACCAACCT TTGTCCCATA CCTGATAAAA GTTCAAGCTC 
     3481 TGAATGATGT GGGGTTTGCA CCAGAGCCAG CTGCAGTCAT GGGGCATTCT GGAGAAGACC 
     3541 TTCCAATGGT GGCTCCTGGA AATGTTCGCG TCAGCGTGGT GAACAGTACG CTGGCAGAGG 
     3601 TGCACTGGGA CCCAGTTCCT CCCAAGAGTG TCCGAGGACA CTTACAAGGC TACCGGATTT 
     3661 ACTACTGGAA GACCCAGAGC TCCTCTAAAA GAAACAGGCG CCACATTGAG AAGAAGATCC 
     3721 TCACCTTCCA GGGCACCAAG ACTCACGGCA TGCTGCCAGG GCTGCAGCCA TACAGTCACT 
     3781 ATGCCCTCAA CGTCCGAGTG GTCAACGGGA AAGGGGAGGG CCCAGCCAGC ACGGACAGAG 
     3841 GCTTCCATAC ACCGGAGGGA GTCCCTAGTG CTCCCTCATC TTTGAAAATT GTTAATCCCA 
     3901 CACTGGACTC CCTCACTTTG GAATGGGACC CCCCAAGCCA CCCAAATGGC ATCCTGACTG 
     3961 AGTACATCTT ACAATATCAG CCAATTAACA GCACACATGA GCTAGGCCCT CTGGTAGATT 
     4021 TAAAAATTCC TGCCAACAAG ACCCGCTGGA CTTTGAAAAA TTTAAATTTC AGCACTCGGT 
     4081 ACAAGTTCTA TTTCTATGCA CAGACATCAG TGGGGCCAGG CAGTCAGATC ACAGAGGAAG 
     4141 CGATAACGAC TGTGGACGAA GCTGGTATTC CTCCACCTGA TGTAGGTGCA GGCAAAGGCA 
     4201 AAGAAGAATG GAGGAAAGAA ATTGTAAATG GTTCTCGAAG CTTCTTTGGG TTAAAGGGTC 
     4261 TAATGCCAGG AACAGCATAC AAAGTTCGAG TTGGTGCTGA GGGGGACTCT GGTTTTGTGA 
     4321 GTTCAGAGGA TGTGTTTGAG ACAGGACCAG CAATGGCAAG CCGGCAAGTG GATATCGCGA 
     4381 CCCAAGGCTG GTTCATAGGT CTAATGTGCG CTGTTGCTCT CCTCATCTTA ATTTTGCTGA 
     4441 TTGTTTGCTT CATCAGAAGA AACAAAGGTG GTAAATACCC AGTTAAAGAA AAGGAGGATG 
     4501 CTCATGCAGA CCCTGAAATC CAGCCCATGA AGGAAGATGA TGGGACGTTT GGAGAATACA 
     4561 GTGATGCAGA AGATCACAAG CCTTTGAAAA AAGGAAGTCG AACACCTTCA GACAGGACTG 
     4621 TGAAAAAAGA AGATAGCGAT GATAGTCTGG TTGACTATGG AGAGGGGGTG AACGGCCAAT 
     4681 TCAACGAGGA TGGCTCCTTT ATTGGCCAAT ACAGTGGTAA GAAAGAGAAA GAGCCAGCAG 
     4741 AGGGAAATGA AAGCTCAGAG GCCCCTTCTC CTGTCAACGC AATGAACTCC TTTGTTACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//