LOCUS       MR219503                7167 bp    DNA     circular  SYN 
DEFINITION  Zdhhc8 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 8 (Zdhhc8)
ACCESSION   NM_172151
VERSION     NM_172151.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3324..3415 
                     /label="Myc-DDK"  
     misc_feature    3445..4030 
                     /label="PolyA signal"  
     misc_feature    3502..3521 
                     /label="Restriction Enzyme"  
     misc_feature    4306..4978 
                     /label="ColE1"  
     misc_feature    5408..6202 
                     /label="Kan/Neo"  
     misc_feature    6237..6539 
                     /label="SV40 ori"  
     misc_feature    6625..7102 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3306..3312 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3306 
                     /label="Zdhhc8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCAGC CCCGGGACGC GCCTCAAACC CGCCAAGTAC ATCCCGGTGG 
     1081 CCACGGCCGC CGCGCTGTTG GTTGGTTCCA GCACACTCTT CTTCGTATTC ACGTGCCCAT 
     1141 GGTTGACAAG AGCTGTGTCT CCAGCTATTC CTGTCTACAA TGGCATCCTC TTCCTCTTTG 
     1201 TCCTGGCCAA CTTCAGTATG GCTACCTTCA TGGACCCTGG AGTCTTCCCC CGAGCGGACG 
     1261 AGGACGAGGA CAAGGAGGAT GACTTCCGGG CCCCACTGTA CAAGAATGTG GATGTGCGGG 
     1321 GCATCCAGGT CCGCATGAAG TGGTGTGCAA CGTGCCACTT TTACCGTCCA CCGCGCTGCT 
     1381 CACACTGCAG TGTCTGTGAC AACTGTGTGG AGGACTTTGA CCATCACTGC CCCTGGGTCA 
     1441 ACAACTGCAT TGGACGCCGC AACTACCGTT ACTTCTTCCT GTTCCTGCTG TCACTCAGCG 
     1501 CGCACATGGT GGGGGTGGTG GCCTTCGGCC TGCTCTATGT GCTCAATCAC TCGGAGGGGC 
     1561 TGGGAGCCGC CCACACCACC ATCACCATGG CTGTCATGTG TGTGGCTGGC CTTTTCTTCA 
     1621 TTCCTGTCAT CGGCCTCACT GGCTTTCACG TGGTACTGGT CACGCGGGGC CGCACCACCA 
     1681 ATGAGCAGGT GACTGGGAAG TTCCGCGGGG GTGTGAATCC CTTCACCCGA GGCTGCTATG 
     1741 GGAACGTGGA GCACGTGTTA TGCAGTCCCC TGGCGCCCCG GTATGTGGTG GAACCCCCCA 
     1801 GGATGCCGCT CTCAGTGAGC CTCAAGCCAC CCTTCCTGAG ACCTGAGCTC CTGGAACGAG 
     1861 CTGTGCCCCT CAAGGTCAAA CTTAGCGACA ATGGGCTGAA AGCTGGCCGC AGCAAGTCCA 
     1921 AGGGCAGTCT AGACCAGCTG GATGAGAAAC CTCTGGACCT GGGACCTCCA CTGCCCCCCA 
     1981 AGATAGAGGC TGGTACCTTT GGAAGAGATC TGAAGACCCC AAGACCTGGC AGTGCTGAGA 
     2041 GTGCCCTATC AGTACAGAGG ACCAGCCCCC CAACACCTGC CATGTATAAG TTCCGGCCAG 
     2101 CCTTCTCCAC TGGTCCCAAG ACACCCTTTT GTGGACCTAA TGAGCAGGTC CCAGGTCCTG 
     2161 ACTCCCTTAC TCTGGCAGAT GACAGCACCC ACAGTCTAGA CTTTGTGTCT GAGCCCAGCC 
     2221 TGGATCTCCC AGACCACGGG CCGGGTGGTC TGCGTCCTCC CTACCCGCCC TCCCCACCCC 
     2281 TCAACACCAC TGATGCCTTC TCAGGTGCCT TGCGCTCCCT GAGTCTCAAG GCTGCCAGTC 
     2341 GGAGGGGTGG GGACCACATG ACCTTACAGC CACTGCGCTC TGAAGGTGGG CCCCCTACAC 
     2401 CTCACCGTAG TCTCTTTGCT CCTCATGCAC TGCCCAATCG AAATGGCAGC CTGTCATATG 
     2461 ACAGCCTACT TAACCCTGGC TCACCCAGTG GCCACGCATG CCCCACACAC CCCTCTGTTG 
     2521 GTATAGCCAG CTACCATTCA CCCTACCTGC ACCCTGGGCC ATCAGATCCA CCGCGGCCCC 
     2581 CACCCCGCAG CTTCAGCCCT GTGCTGGGTC CCCGGCCTAG GGAACCCTCT CCTGTGCGCT 
     2641 ATGACAACCT GTCTCGGACC ATCATGGCCT CTATCCAGGA GCGCAAGGAC AGGGAAGAGC 
     2701 GTGAACGGCT GCTGCGCTCC CAGACTGACT CACTCTTTGG CGACTCTGGT GTCTATGATA 
     2761 CACCCAGCTC CTACAGCCTG CAACAGGCCA GTGTGTTAAC GGAAGGCCCC CGTGGCTCTG 
     2821 TCCTGCGTTA CGGCTCCAGG GATGACCTCG TGGCCGGGCC TGGCTTTGGT GGTGCCCGCA 
     2881 ATCCTGCCCT GCAGACGTCA TTGTCCTCGC TGTCCAGCTC CATGAGTCGG GCACCTCGGA 
     2941 CATCTTCCTC CTCCCTGCAG GCTGACCAGG CCAACAACAA TGCCCCAGGA CCCCGGCCTG 
     3001 GCAGTGGTTC ACATAGGTCA CCTGCCCGTC AGGGCCTGCC TTCCCCACCA GGCACCCCCC 
     3061 GATCGCCCTC CTACACGGGC TCCAAGGCCG TCGCCTTCAT CCACACAGAC CTCCCGGACC 
     3121 GGCAGCCCTC ACTGGCTATG CAGAGGGATC ACCCTCAGCT GAAGACCCCC CCAAGTAAGC 
     3181 TTAACGGGCA GTCCCCAGGC ATGGCCCGTC TGGGGCCTGC TGCCAGCCCC ATGGGGCCCA 
     3241 ACGCCAGCCC TGCCCGGCAC ACGCTGGTTA AGAAGGTGTC CGGCGTGGGT GGGACTACGT 
     3301 ATGAAATCTC GGTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3361 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3421 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3481 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3541 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3601 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3661 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3721 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3781 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3841 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3901 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3961 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4021 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4081 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4141 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4201 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4261 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4321 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4381 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4441 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4501 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4561 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4621 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4681 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4741 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4801 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4861 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4921 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4981 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5041 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5101 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5161 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5221 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5281 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5341 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5401 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5461 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5521 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5581 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5641 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5701 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5761 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5821 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5881 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5941 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6001 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6061 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6121 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6181 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6241 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6301 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6361 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6421 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6481 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6541 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6601 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6661 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6721 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6781 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6841 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6901 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6961 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7021 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7081 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7141 ATTTAACAAA AATTTAACGC GAATTTT
//