LOCUS       MR219495                7218 bp    DNA     circular  SYN 
DEFINITION  Sik1 (Myc-DDK-tagged) - Mouse salt inducible kinase 1 (Sik1)
ACCESSION   NM_010831
VERSION     NM_010831.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3375..3466 
                     /label="Myc-DDK"  
     misc_feature    3496..4081 
                     /label="PolyA signal"  
     misc_feature    3553..3572 
                     /label="Restriction Enzyme"  
     misc_feature    4357..5029 
                     /label="ColE1"  
     misc_feature    5459..6253 
                     /label="Kan/Neo"  
     misc_feature    6288..6590 
                     /label="SV40 ori"  
     misc_feature    6676..7153 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3357..3363 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3357 
                     /label="Sik1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGATCATG TCGGAGTTCA GTGCGGTCCC CTCAGGCACT GGCCAGGGCC 
     1081 AGCAGAAGCC CCTCCGGGTG GGCTTTTACG ACGTGGAACG GACCCTGGGC AAAGGCAATT 
     1141 TTGCAGTGGT TAAGCTGGCG CGGCACCGAG TCACCAAAAC GCAGGTTGCA ATAAAAATAA 
     1201 TTGACAAGAC ACGGTTAGAT TCTAGCAATC TGGAGAAGAT CTACCGGGAG GTCCAGCTCA 
     1261 TGAAACTTTT GAACCACCCA AATATCATCA AGCTTTATCA GGTTATGGAG ACAAAGGATA 
     1321 TGCTCTACAT TGTCACAGAA TTTGCAAAAA ATGGAGAAAT GTTTGATTAT CTGACTTCCA 
     1381 ACGGGCACTT GAGTGAAAAC GAGGCTAGGC AGAAGTTCTG GCAGATTCTG TCAGCCGTGG 
     1441 AGTACTGCCA CAACCACCAC ATTGTCCACC GGGACCTCAA GACGGAGAAC CTGCTCCTGG 
     1501 ACAGCAACAT GGATATCAAG CTAGCAGATT TTGGATTTGG GAATTTCTAC AAGCCAGGCG 
     1561 AGCCTCTGTC CACGTGGTGT GGGAGCCCCC CGTATGCAGC CCCTGAAGTC TTCGAGGGGA 
     1621 AGGAGTACGA GGGTCCCCAG CTGGACGTCT GGAGCCTCGG TGTGGTGCTG TACGTCCTGG 
     1681 TCTGTGGCTC CCTCCCCTTC GACGGGCCCA ACCTGCCTAC GCTGAGACAG CGGGTACTGG 
     1741 AGGGCCGCTT CCGCATCCCC TTCTTCATGT CTCAAGACTG TGAGACGCTG ATCAGACGCA 
     1801 TGCTGGTCGT GGACCCCGCT AAGCGCATCA CCATAGCCCA GATCCGCCAG CACCGGTGGA 
     1861 TGCAGGCCGA CCCCACTCTC CTGCAGCAGG ATGACCCTGC CTTCGACATG CAAGGCTACA 
     1921 CCTCCAACCT GGGCGACTAC AACGAACAGG TGCTAGGGAT CATGCAGGCC CTCGGCATCG 
     1981 ACCGGCAGCG GACTATAGAG TCTCTGCAGA ACAGCAGCTA CAACCACTTT GCCGCCATTT 
     2041 ACTACCTCCT ACTCGAGCGC CTCAAGGAGC ATCGAAGCGC CCAGCCCTCA TCCCGGCCCA 
     2101 CCCCTGCACC CACCAGACAG CCCCAGCTCC GAAGCTCAGA CCTCAGCAGT CTGGAGGTTC 
     2161 CTCAAGAAAT TCTCCCGTGT GACCCTTTCC GGCCCTCTCT GCTGTGCCCA CAGCCCCAGG 
     2221 CCTTGGCTCA GTCTGTCCTG CAGGCTGAGA TAGACTGTGA TCTCCACAGC TCACTTCAGC 
     2281 CCTTATTATT CCCCCTGGAT ACCAACTGCA GTGGAGTGTT CCGGCACCGA TCCATCTCCC 
     2341 CCAGCAGTCT GCTGGACACA GCTATCAGCG AGGAGGCCAG GCAGGGTCCC AGCCTAGAGG 
     2401 AGGAACAGGA GGTCCAGGAA CCCCTGCCCG GAAGCACAGG CCGGAGGCAC ACATTGGCTG 
     2461 AAGTCTCCAC CCATTTCTCC CCGCTCAACC CTCCTTGCAT AATTGTCTCC TCCTCTGCCA 
     2521 CGGCGAGTCC CTCGGAAGGG ACCAGCTCCG ACAGCTGCCT CCCCTTCTCT GCAAGTGAAG 
     2581 GTCCTGCAGG GCTTGGCAGT GGCCTGGCCA CCCCAGGGCT GCTGGGCACC AGCTCTCCAG 
     2641 TCAGATTGGC CTCGCCCTTC CTGGGATCAC AGTCAGCCAC CCCTGTGCTC CAGACTCAGG 
     2701 CAGGTCTGGG CACAGCCGTC TTACCTCCCG TCAGCTTCCA GGAGGGACGG AGAGCGTCTG 
     2761 ATACCTCTCT CACTCAGGGG CTGAAGGCCT TCCGGCAGCA GCTGAGGAAA AATGCGAGGA 
     2821 CCAAGGGGTT CCTGGGACTG AACAAGATCA AAGGGTTGGC TCGCCAGGTG TGCCAGTCCT 
     2881 CCGTCCGAAC TCCCCGGGGA GGGATGAGTA CCTTCCACAC CCCAGCCCCA AGCTCCGGCC 
     2941 TGCAAGGCTG CACGACCAGC AATCGGGAGG GCAGGAGCCT ACTTGAAGAG GTCCTGCACC 
     3001 AGCAGAGGCT GCTCCAGTTA CAGCACCACT CTTCTACCGC CGCCGCCTCA TCTGGCTGCC 
     3061 AGCAGGGCCC CCAGCTGTCC CCGGTCCCCT ATGTGCTTGC TCCCTGTGAC AGCCTCCTCG 
     3121 TGTCTGGAAT TCCACTGCTG CCAACACCCC TTCTCCAGGC TGGCATGTCC CCTGTGGCGT 
     3181 CTGCTGCGCA TCTCCTGGAT ACCCACTTGC ACATCAGTGC TGGCCCAGTG GCCCTCCCCA 
     3241 CTGGGCCCCT GCCACAGTGC CTCACCAGGC TGTCCCCAGG CTGTGATCCA GCTGGGCTGC 
     3301 CACAGGGGGA CTGTGAGATG GAGGACCTGA CCTCTGGCCA GCGGGGGACA TTCGTCCTGG 
     3361 TACAGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3421 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3481 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3541 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3601 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3661 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3721 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3781 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3841 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3901 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3961 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4021 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4081 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4141 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4201 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4261 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4321 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4381 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4441 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4501 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4561 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4621 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4681 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4741 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4801 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4861 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4921 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4981 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5041 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5101 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5161 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5221 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5281 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5341 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5401 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5461 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5521 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5581 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5641 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5701 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5761 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5821 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5881 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5941 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6001 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6061 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6121 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6181 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6241 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6301 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6361 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6421 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6481 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6541 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6601 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6661 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6721 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6781 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6841 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6901 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6961 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7021 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7081 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7141 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7201 AAATTTAACG CGAATTTT
//