LOCUS       MR219472                8391 bp    DNA     circular  SYN 
DEFINITION  Maml2 (Myc-DDK-tagged) - Mouse mastermind like 2 (Drosophila) (Maml2), transcript variant 1. Note: ORF is codon optimized
ACCESSION   NM_001013813
VERSION     NM_001013813.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4548..4639 
                     /label="Myc-DDK"  
     misc_feature    4669..5254 
                     /label="PolyA signal"  
     misc_feature    4726..4745 
                     /label="Restriction Enzyme"  
     misc_feature    5530..6202 
                     /label="ColE1"  
     misc_feature    6632..7426 
                     /label="Kan/Neo"  
     misc_feature    7461..7763 
                     /label="SV40 ori"  
     misc_feature    7849..8326 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4530..4536 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4530 
                     /label="Maml2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGACACA GCGCCTCCGC AGGCTCCAGC AGGAGGGTTG GGAGGTGCTT 
     1081 CTGGAGCGGG TCTCCTTGGA GGTGGCTCAG TCACTCCGAG AGTGCACAGT GCTATTGTGG 
     1141 AACGCCTGCG GGCTCGCATT GCTGTCTGCC GACAGCACCA CCTGAGCTGT GAAGGACGCT 
     1201 ATGAACGTGG TAGGGCTGAG AGCTCAGACC GAGAGAGGGA GAGCACCATG CAGCTTCTCA 
     1261 GCCTGGTGCA GCATGGTCAG GGTGCAAGGA AGGCTAGCAA ACATGCTAAG GCTACAGTCG 
     1321 CGACTGCCAC TACCACAGCT CCTCCACCGC CCCCTACTGC CCCTCCTGTG GCCTCCCAAG 
     1381 CGGCAGCCAC GGCCCCACCA CCTCCACCAG ACTATCACCA TCACCATCAG CAGCACCGGC 
     1441 TGACCAGTAA TGATAACGGT GGGATAAACC GAGAGCAGCC GCCAGCTTCG ACCCCGGGGG 
     1501 ACCAGCGGAA CTCAGCCCTG CTTGCGCTCC AGGGCTCTTT GAAAAGAAAG CAAATAGTCA 
     1561 ACTTGTCTCC TGCCAACAGC AAGAGGCCCA ATGGCTTTGT TGACAGCTCA TTTCTTGACA 
     1621 TCAAAAGGAT CAGAGTTGGG GAGAACCTTT CTACAGGTCA GGGGGGCCTC CCAGTAAATA 
     1681 ATGGACAAAG TCAAATGATG CCAGGGCCCT TGACCATGAA TCAAGCACCC CTGAGGAAGA 
     1741 CTAATGCTCT ACCACCTCCT GCACATTCCC CTGGCAACAA TTTGTTTAAC ATGGGCTTAA 
     1801 AGGAGGTGAA GAAAGAACCT GGGGAGACCC TGTCCTGCAG TAAGCACATG AGTGACCAAA 
     1861 TGACACAAGA GAGTGTTTTT ACTAATAGGT TTGGAGATGA CCCTGGAGAT CAACTGATGG 
     1921 ACCCTGAGCT ACAGGAACTG TTCAATGAAC TAACCAACAT ATCAGTGCCT CCCATGAGTG 
     1981 ACCTGGAGCT GGAGAACATG ATCAATGCTA CCATTAAGCA AGATGACCCG TTTGGCATTG 
     2041 ACTTGGGTCA GCAGAGCCAG AGGAGCACAC CTAGACCCTC CCTGCCCATG GAGAAGATCA 
     2101 TTATCAAAAG TGAATATTCA CCTGGTTTGA CACAAGGCAC TTCAGGCTCT CCCCAGCTGA 
     2161 GACCCCCATC AGCTGGCCCT GCATTTTCCT TGGCTAACTC TGCACTCTCC ACTTCATCCC 
     2221 CAATTCCCAC AATTCCCCAG AGTCAGCCTC AGCTTCAGAC AGTATCAGGA GCAAACAGGG 
     2281 CCCTGTCAAG CTGGCAAGAA GTATCCCATG CTCAACAGCT CAAGCAGATA GCTGCAAACC 
     2341 GCCAGCAGCA TGCACGGATG CAGCAGCAGC AACAGCAGCA GCAGCAGCAG CCATCTCCCA 
     2401 GCTGGCCAGC CTTGCAGTCC TCTGCTGGGC CATCCCCAGG AGCATTTGTG CAGGAAAAAA 
     2461 TCCCTAGCCC TTCCTTCAGC CAGCAACCAT TCAGTCCACA GGGATCCCCC ATGCCTGGGG 
     2521 TGACTGGCAG CAACACCCAG TCTAAAGCAG CGGCCAATTA CACGTACAAG GCCAGCCCCT 
     2581 CAGCCCAGGC TGGACACCTG GATGTGCTTC TGCAGCAAAA GCCTCAGGAG CTCAATCGAA 
     2641 GCTTCATTAA TAACCCACAC CCAGCCTTGG AGCCCCGGCA TGGCAATACC AAGCCTTTGT 
     2701 TCCATTTTAA CTCAGACCAA GCAAACCAGC AGATGCCTTC TCTTTTGCCT TCCCAGAGCA 
     2761 AACCTTCTCT CTTGCACTAT ACCCAACAGC AGCAGCAACA GCAGCAGCAA CAACAGCAAC 
     2821 AGCAACAACA GCAACAACAG CAACAGCAAC AACAGCAGCA GCAACAACAA CAGCAGCAAC 
     2881 AACAACAGCA ACAGCAGCAA CAGCAACAAC AACAGCAGCA AGGCTCCCTG GCAGCTCAAC 
     2941 AGCAACAGCA GCAGCCACAG CCACAGAGTT CATTGGTAGC TCAGCAGCAG CAACAGCAGC 
     3001 AGCAACAGCA GCAGCAACAG CAAGGTTCAT TGACAGCTCA ACAACAGCAG CAGCAGCAAC 
     3061 AACAGCAGCA ACCATCGCAA CCCACCCATG CTTTATCAAG CCAGCCTTTG CTAAGGTCAC 
     3121 CCTTACAACT TCAGCAAAAG ATCATGCTTC AGAAAATGCA AACTCAGTCC ATCACAGGCC 
     3181 TGGGATATCA AGTCACTCAA CAGCACAGAC AGGATCAACA CACTGTGGTA GGCCAGAACA 
     3241 CAGGCCCCAG TCCAAGTCCC AACTCCTGCT CAAATCCAAA CACTGGAAGT GGTTACATGA 
     3301 ACTCCCAGCA GTCCCTGTTG AATCAGCAGA TGATGGGGAA GAAACAGACC CTGCAGAGAC 
     3361 CAACCATGGA GCAGAAGCAG CAACTTCTCC TCCAGCAGCA GATGCTGGCT GATGCGGAGA 
     3421 AACTTTCCCC TCAGGATCAG ATGAACAGAC ATCTGACAAG GCCGCCCCCA GACTACAAAG 
     3481 ACCAAAGAAG AAATACGGGC ACCCTGCAGC CAGCTGCTCA GTATTCGGGT GGCTCCTCTA 
     3541 CAGTGAGTTT AAACTCTAAC CAGGCTCTGA CAAACCCAGT TTCAACACAC ACCATTTTAA 
     3601 CTCCAAACTC CAGCCTCATG TCTACGTCTC ATGGGATGAG AATGCCATTG TTATCTACAG 
     3661 TTCAGAACAT AGGGATGTAT GGAAACCTGC CTTGTAACCA GCCTGGCACC TACAACGTCA 
     3721 CTTCAACAAT GAACCAGCTG ACACAACAGA GAAATACAAC TCAACTGATA ACAAATCAGA 
     3781 ACAACCCTCT GATGTCTCGG CCATCTCCCT TAGGGGCAAA TAACGGTAAC AATGTGGCCA 
     3841 CCTTTGGAGC TGGATCTGCT GGCAGTTCAC AGCAATTGAG ACCAAATTTG GCTCACAGTT 
     3901 TGTCAGGCAT GTCAGCCCAG AGGTCATCAA CTGTCATGAT CACAGCCAAC ACGACAGCAA 
     3961 CAAACTGGGC TTCTCAAGAG GTGACAGGGA AACAACAGGA AGCCCTCAAA TCCACAGGAG 
     4021 TCCGGTTCCC AACAAGTACA CCTGCAGCCT ATACCCCAAA CCAGTCTTTG CAACCAGGAG 
     4081 TAGGGAGCCA GCCATTTTCC CAGAGGGCAG TGGCACCCCC TAGCCAGTTA TCGCCAGCAG 
     4141 TGCAAATGAG ACCTATGAAC CAAATGAATC AGGCATTAAA TGGGCAAACC CTGGGTTCAC 
     4201 TCAGGGGGTT GAACCTCAGA CCCAATCAGC TGGCCGCCCA GAGCCTGTCT AATATGAACC 
     4261 CATCAGGGAC AGGATTGAAT CATCCCAGGA CAGGCACCAA CCAGCCTCCA TCCCTGACAC 
     4321 CTAACGCTTT TCCTTCATCC AACCAAAGTT CCAGGGCTTT TCAAGGACCT GATCATGGCA 
     4381 GTGATTTAGC TTTTGACTTC CTCAGCCAAC AGAGCGACAG CATGTGCCCT GCCCTAAACA 
     4441 GTGATGCTGA TTTCATCGAT TCTTTATTGA AGACAGAGCC TGGTAATGAC GACTGGATGA 
     4501 AAGATATCAA CCTTGATGAG ATCCTGGGAA GCAATTCCAC GCGTACGCGG CCGCTCGAGC 
     4561 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4621 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4681 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4741 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4801 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4861 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4921 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4981 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5041 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5101 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5161 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5221 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5281 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5341 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5401 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5461 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5521 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5581 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5641 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5701 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5761 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5821 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5881 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5941 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6001 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6061 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6121 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6181 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6241 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6301 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6361 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6421 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6481 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6541 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6601 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6661 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6721 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6781 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6841 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6901 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6961 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7021 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7081 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7141 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7201 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7261 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7321 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7381 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7441 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7501 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7561 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7621 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7801 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7861 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7921 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7981 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8041 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8101 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8161 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8221 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8281 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8341 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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