LOCUS       MR219455                7599 bp    DNA     circular  SYN 
DEFINITION  Tlr12 (Myc-DDK-tagged) - Mouse toll-like receptor 12 (Tlr12)
ACCESSION   NM_205823
VERSION     NM_205823.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3756..3847 
                     /label="Myc-DDK"  
     misc_feature    3877..4462 
                     /label="PolyA signal"  
     misc_feature    3934..3953 
                     /label="Restriction Enzyme"  
     misc_feature    4738..5410 
                     /label="ColE1"  
     misc_feature    5840..6634 
                     /label="Kan/Neo"  
     misc_feature    6669..6971 
                     /label="SV40 ori"  
     misc_feature    7057..7534 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3738..3744 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3738 
                     /label="Tlr12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGGTAC TGGCTGCTGC CAGGTCTCCT CCTTTCCCTG CCTCTGGTAA 
     1081 CTGGGTGGAG CACTTCCAAC TGCCTGGTGA CCGAAGGCTC CCGACTGCCC CTGGTCTCCC 
     1141 GCTATTTCAC ATTCTGCCGC CATTCCAAGC TATCCTTTCT TGCTGCATGC CTCTCCGTGA 
     1201 GCAACCTGAC ACAGACCTTG GAAGTTGTAC CTCGGACTGT GGAGGGGCTC TGCCTCGGTG 
     1261 GTACTGTGTC TACTCTGCTT CCAGATGCTT TCTCTGCTTT TCCTGGTCTC AAGGTCCTGG 
     1321 CACTGAGTCT GCACCTTACC CAACTTCTGC CAGGAGCTCT CCGGGGTCTG GGACAGTTGC 
     1381 AGAGCCTCTC TTTTTTTGAC TCTCCTCTTA GGAGATCTCT CTTTCTACCT CCTGATGCCT 
     1441 TCAGTGACCT GATTTCCCTC CAGAGACTCC ATATCTCTGG CCCTTGCCTG GATAAGAAGG 
     1501 CAGGCATCCG CCTGCCTCCC GGTCTGCAAT GGCTGGGTGT CACGCTCAGT TGCATTCAGG 
     1561 ACGTGGGAGA GCTGGCTGGT ATGTTCCCAG ATCTGGTGCA AGGTTCCTCC TCCAGGGTTT 
     1621 CGTGGACCCT GCAGAAGTTG GATCTGTCAT CCAACTGGAA GCTGAAGATG GCTAGTCCTG 
     1681 GGTCCCTCCA GGGTCTCCAG GTGGAGATTC TGGACCTGAC AAGAACACCA CTGGATGCCG 
     1741 TGTGGCTGAA GGGCCTGGGA CTTCAGAAAC TCGATGTCTT GTATGCACAG ACTGCCACGG 
     1801 CCGAGCTGGC TGCTGAGGCT GTTGCCCACT TTGAGCTGCA GGGCTTGATT GTGAAAGAAA 
     1861 GCAAGATAGG ATCTATATCT CAGGAGGCTC TGGCTTCCTG CCACAGCCTG AAGACCTTGG 
     1921 GTCTTTCAAG CACTGGCCTA ACCAAGCTTC CACCAGGCTT CCTGACTGCC ATGCCTAGGC 
     1981 TTCAGCGACT GGAGCTGTCC GGAAACCAAC TGCAGAGCGC CGTGCTGTGC ATGAATGAGA 
     2041 CGGGAGATGT GTCAGGACTC ACGACTCTGG ATCTGTCAGG CAACAGGTTG CGCATCCTGC 
     2101 CTCCAGCCGC CTTCTCCTGC TTACCCCACT TGCGAGAGCT GCTGCTTCGG TACAACCAGC 
     2161 TGCTTTCCCT GGAGGGATAC CTATTCCAGG AGCTCCAGCA ACTAGAGACC TTGAAGCTGG 
     2221 ATGGAAACCC CCTGCTTCAC CTGGGTAAGA ACTGGTTGGC GGCTCTGCCT GCATTGACCA 
     2281 CCCTTAGCTT GCTAGATACC CAAATACGGA TGAGCCCAGA GCCTGGCTTC TGGGGAGCAA 
     2341 AGAATCTGCA TACCTTGAGC CTGAAGCTTC CCGCTCTCCC TGCTCCGGCA GTATTGTTCC 
     2401 TGCCCATGTA TCTGACCAGC TTAGAGCTTC ATATAGCCTC AGGCACGACG GAGCACTGGA 
     2461 CGCTGTCCCC AGCGATCTTT CCTTCCTTGG AGACCTTGAC TATAAGCGGC GGGGGACTGA 
     2521 AGCTGAAGCT GGGGTCCCAG AATGCTTCTG GGGTCTTCCC TGCTCTCCAG AAGCTCTCCC 
     2581 TGCTTAAGAA CAGCTTGGAT GCCTTCTGCT CCCAGGGTAC CTCCAACCTT TTCCTCTGGC 
     2641 AGCTCCCCAA ACTTCAGTCC TTGAGGGTAT GGGGTGCTGG AAACAGCTCC AGACCCTGCC 
     2701 TTATCACTGG GCTGCCCAGC CTACGGGAGC TGAAGCTGGC GTCGCTTCAG TCCATAACCC 
     2761 AGCCCCGTTC GGTGCAGCTG GAGGAGCTGG TGGGTGACCT TCCACAGCTC CAGGCCTTAG 
     2821 TGCTATCCAG CACAGGCCTC AAGTCACTGT CGGCTGCTGC TTTCCAGCGC CTGCACAGTC 
     2881 TCCAGGTCTT AGTGCTAGAA TACGAGAAGG ACTTGATGCT GCAGGACAGT CTGAGGGAGT 
     2941 ACAGCCCTCA GATGCCCCAC TATATATACA TTCTGGAGTC AAACCTGGCC TGCCACTGTG 
     3001 CCAATGCGTG GATGGAGCCA TGGGTTAAGC GGTCCACTAA AACGTACATA TACATAAGAG 
     3061 ACAATCGCTT ATGTCCAGGA CAAGACAGGC TCTCTGCTAG GGGTTCCCTT CCCTCCTTTC 
     3121 TCTGGGACCA CTGCCCCCAG ACGTTGGAGC TGAAACTCTT TTTGGCTAGT TCTGCCTTGG 
     3181 TGTTCATGCT AATTGCCTTG CCTCTCCTCC AAGAAGCCAG GAACTCTTGG ATCCCCTACC 
     3241 TGCAGGCCTT GTTCAGGGTT TGGCTCCAGG GTCTGAGGGG TAAGGGAGAC AAGGGGAAGA 
     3301 GGTTCCTTTT TGATGTATTC GTGTCCCACT GCAGGCAAGA CCAGGGCTGG GTGATAGAGG 
     3361 AACTTCTGCC TGCTCTGGAG GGCTTCCTTC CAGCTGGCCT GGGCCTGCGC CTCTGTCTCC 
     3421 CCGAGCGTGA CTTTGAGCCT GGTAAGGATG TAGTTGATAA TGTGGTAGAT AGCATGTTGA 
     3481 GCAGCCGTAC CACACTCTGC GTGTTGAGTG GGCAGGCCCT GTGTAACCCC CGATGCCGCC 
     3541 TGGAGCTCCG CTTGGCCACC TCTCTCCTCC TGGCTGCCCC GTCCCCTCCA GTGTTGCTGC 
     3601 TAGTCTTCTT GGAACCCATT TCTCGGCACC AGCTTCCGGG TTACCACAGA CTGGCTCGGC 
     3661 TGCTTCGAAG AGGAGACTAC TGTCTGTGGC CCGAGGAAGA GGAGAGAAAG AGTGGGTTCT 
     3721 GGACTTGGCT GAGGAGCAGG CTAGGGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3781 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3841 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3901 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3961 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4021 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4081 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4141 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4201 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4261 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4321 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4381 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4441 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4501 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4561 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4621 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4681 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4741 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4861 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4921 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4981 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5041 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5161 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5221 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5281 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5341 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5401 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5461 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5521 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5581 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5641 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5701 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5761 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5821 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5881 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5941 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6001 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6061 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6121 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6181 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6241 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6301 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6361 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6421 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6481 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6541 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6601 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6661 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6721 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6781 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6841 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6901 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6961 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7021 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7081 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7141 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7201 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7261 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7321 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7381 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7441 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7501 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7561 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//