LOCUS       MR219447                6741 bp    DNA     circular  SYN 
DEFINITION  Vgf (Myc-DDK-tagged) - Mouse VGF nerve growth factor inducible (Vgf)
ACCESSION   NM_001039385
VERSION     NM_001039385.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2896..2987 
                     /label="Myc-DDK"  
     misc_feature    3017..3602 
                     /label="PolyA signal"  
     misc_feature    3074..3093 
                     /label="Restriction Enzyme"  
     misc_feature    3878..4550 
                     /label="ColE1"  
     misc_feature    4980..5774 
                     /label="Kan/Neo"  
     misc_feature    5809..6111 
                     /label="SV40 ori"  
     misc_feature    6197..6674 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2871..2878 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2871 
                     /label="Vgf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAACCTTC ACGTTGCCGG CATCCGTCCT CTTCTGCTTC CTTCTACTGA 
     1081 TCCAGGGGTT GGGAGCAGCG CCCCCCGGGC GCCCCGATGT TTTTCCTCCT CCCCTCAGCT 
     1141 CTGAGCATAA TGGGCAGGTA GCTGAGGACG CAGTGTCCCG GCCAAAGGAT GACGGCGTAC 
     1201 CAGAGGTCCG AGCTGCTCGG AATCCCGAGC CTCAGGACCA GGGAGAGCTC TTCCAGGGCG 
     1261 TGGATCCCCG GGCGCTGGCC TCGGTACTGT TGCAGGCACT GGACCGTCCG GCCTCGCCCC 
     1321 CGTCGGTCCC GGGAGGTTCC CAGCAGGGAA CACCCGAAGA AGCAGCAGAA GCTCTGCTGA 
     1381 CCGAGTCCGT GCGCAGTCAG ACCCATAGCC TCCCGGCACC AGAAATCCAA GCGCCCGCTG 
     1441 TGGCCCCCCC TCGCCCTCAG ACTCAGGACC GCGATCCCGA GGAGGACGAC CGCTCAGAAG 
     1501 AGCTGGAGGC GCTAGCATCC TTGCTCCAAG AACTTCGAGA TTTCAGTCCG AGCAATGCTA 
     1561 AGCGCCAACA AGAGACGGCG GCAGCAGAGA CTGAAACCCG CACGCACACG CTGACCCGAG 
     1621 TCAATCTGGA GAGCCCCGGG CCAGAGCGCG TGTGGCGCGC TTCCTGGGGA GAGTTCCAGG 
     1681 CGCGCGTTCC GGAGCGCGCT CCTCTTCCGC CCCCGGTCCC TTCCCAATTC CAGGCTCGAA 
     1741 TGTCCGAAAG CGCTCCCCTT CCCGAAACTC ATCAGTTCGG GGAAGGAGTG TCCTCCCCTA 
     1801 AAACACATTT AGGTGAGACT TTGACACCCT TATCCAAGGC GTATCAAAGT CTAGGTGGCC 
     1861 CCTTCCCTAA GGTGCGCCGG CTCGAGGGCT CATTCCTGGG CGGCTCCGAG GCAGGAGAGC 
     1921 GCCTGCTTCA GCAAGGATTA GCTCAGGTAG AGGCAGGGAG GAGACAGGCG GAGGCCACCC 
     1981 GGCAGGCCGC GGCGCAAGAA GAGCGGCTGG CCGATCTCGC CTCCGACCTG CTGCTCCAGT 
     2041 ATCTGTTGCA GGGCGGAGCC CGGCAGCGCG ATCTCGGGGG TCGCGAGCTG CAGGAGACGC 
     2101 AGCAAGAGCG GGAGAACGAG AGGGAGGAGG AGGCAGAGCA GGAGAGGCGA GGTGGTGGGG 
     2161 AGGACGATGT GGGGGAAGAG GATGAGGAGG CGGCGGAGGC GGAGGCGGAG GCAGAGGAGG 
     2221 CGGAGAGGGC GCGGCAGAAC GCACTCCTGT TCGCCGAGGA GGAGGACGGG GAAGCCGGAG 
     2281 CCGAGGACAA ACGCTCCCAG GAGGAGGCGC CAGGCCATCG GCGGAAGGAT GCTGAGGGGG 
     2341 CAGAGGAGGG CGGGGAGGAG GATGACGACG ACGAGGAGAT GGATCCGCAG ACGATCGATA 
     2401 GTCTCATTGA ACTGTCCACC AAACTCCACC TGCCAGCAGA CGATGTGGTC AGCATCATCG 
     2461 AAGAGGTGGA GGAGAAACGG AAGCGGAAGA AGAACGCCCC TCCCGAGCCG GTGCCGCCTC 
     2521 CCAGGGCTGC CCCAGCCCCG ACCCATGTCC GCTCTCCGCA GCCCCCACCT CCCGCCCCAG 
     2581 CCCGGGATGA GTTGCCGGAC TGGAACGAAG TGCTCCCACC CTGGGATCGG GAGGAGGATG 
     2641 AGGTGTTTCC CCCGGGGCCC TATCACCCCT TCCCAAACTA CATTCGGCCG CGGACACTGC 
     2701 AGCCGCCCGC ATCCTCCCGC CGCCGTCACT TCCATCACGC GTTGCCACCT GCGCGCCACC 
     2761 ATCCGGATCT GGAGGCCCAG GCCAGGCGCG CGCAGGAGGA AGCGGACGCG GAGGAGCGCC 
     2821 GGCTGCAGGA GCAGGAGGAG CTGGAGAATT ACATTGAGCA CGTGCTGCTG CACCGCCCGA 
     2881 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     2941 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3001 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3061 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3121 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3181 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3241 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3301 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3361 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3421 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3481 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3541 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3601 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3661 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3721 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     3781 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     3841 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     3901 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     3961 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4021 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4081 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4141 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4201 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4261 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4321 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4381 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4441 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4501 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4561 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4621 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4681 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4741 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     4801 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     4861 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     4921 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     4981 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5041 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5101 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5161 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5221 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5281 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5341 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5401 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5461 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5521 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5581 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5641 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5701 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     5761 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     5821 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     5881 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     5941 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6001 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6061 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6121 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6181 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6241 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6301 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6361 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6421 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6481 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6541 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6601 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6661 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6721 CAAAAATTTA ACGCGAATTT T
//