LOCUS       MR219435                9399 bp    DNA     circular  SYN 
DEFINITION  Pik3c2g (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, C2 domain containing, gamma polypeptide (Pik3c2g), transcript variant 1
ACCESSION   NM_207683
VERSION     NM_207683.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5556..5647 
                     /label="Myc-DDK"  
     misc_feature    5677..6262 
                     /label="PolyA signal"  
     misc_feature    5734..5753 
                     /label="Restriction Enzyme"  
     misc_feature    6538..7210 
                     /label="ColE1"  
     misc_feature    7640..8434 
                     /label="Kan/Neo"  
     misc_feature    8469..8771 
                     /label="SV40 ori"  
     misc_feature    8857..9334 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5538..5544 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5538 
                     /label="Pik3c2g"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATACAGT TGGCAAACAG AGCCAAATCG CACTGAACCA CAGGAAGATG 
     1081 GATCTGATAC CCAACAATTT CACCATACAA ACCAGCACCT TTCTTCCAGG CAAGTCAGGT 
     1141 TGGGTTTTGA TCAGCTGGTG GAAGAGATCA ATAATAAGAC TCCACTGTCT GAGAGTGAAA 
     1201 AGGAAGAAGA CACGTATTTT GTGCCAGATG CACCAAACTT GGGTTCAAAA TGGCCATCCA 
     1261 TATATGAAAC ACACCCAAGA TACTTCAGTG AATTCACTTC TCAGAGCCCT GACTCCTCCC 
     1321 AGCTTCGGTT TGGAAAACTA TCAGCCATTG GTTTTAATCC TGCTGTACTA CCAACCCATC 
     1381 AGCTCATTCA TGAGGGAGCT TCCTGGAGAA ATCCCAGTGG AAAATATCAT GGCATCGAGT 
     1441 ATCCCAGATT CGATGCTCTA CCTCCATCAT CCACTGGCCA GGGTGAGTGT AATCCACAAG 
     1501 GACAATCAGG AACCAAACAT CATAACTACT GTGGAGAACA TGAAGGCAAT CTCCCCCATC 
     1561 ATCATTCATC CTACTCAATA GACTCCATAC CGAATAGAGA GAAAAGAAGG AGTGGAGATG 
     1621 TGAACCTTGT AGAACCTTCT CTGGAGTTCT CTAAAGACTC CTTTCTACCC AGGACATCGG 
     1681 AGAACGTGTC AGTGGAAAGC ACAGAGCCGA TTGGTTGTCC CATTGAAATA GTTGAGGTAC 
     1741 CCCAAGGGAG TAACAAGAAC CTGGCCTCCT TTTGTAACAA AGTAAAAAAG ATAAGAGAAT 
     1801 CATACCATGC AAGTGACATT AATTCCAATT CTGGGAAGAT CTGGGCCATC ACCACAGCCT 
     1861 ATCCATCTCG GCTCTTCGCT GACACCAAGT TCAGAGTAAA AATTTCCATT GATAACTCAG 
     1921 CCCAACTCCT CCTTCTTATG CCACACGCTA ATTATCTTGT CAAAGACCTA ATTGCTGAAA 
     1981 TTCTACTTTT ATGTGCAAAT GAGCCGCTTT CCCCCAAAGA GTATCTTCTA AGTGTATGCG 
     2041 GTTCTGAGGA ATTTTTACAG ATGGATCACT CTCTAGGGAG CCACAAAATA TTCCAGAAAA 
     2101 ATAAATCTGT CATTCAACTT CATCTTCAGA AAAACAGGGA CACTCCAGGA AAATTATCCC 
     2161 GGAAGAGTGA AGATGACCAC AGTCCGTTTC ATCTGAACCA ACTTCTAGAA TTTACACATA 
     2221 TCTGGAAAAT ATCCAGACAA TGCCTCTCCA CAGTAATGAA AAAATACAAC CTCCACGTGG 
     2281 AACACCTGTT GAAACCCCAG AAAGACATGG AGGAGAAACA TTTGTCGTCC ATGGTTTCTG 
     2341 GTAACCAACA CACTTCTCAG CCACATGTGA ATAATGTTCT TGAAGAAGTT AAAAACATAT 
     2401 GCAGTGTTTT GGGGTGTATT GAAACCAAAC AAGTTTCAGA TGCCGTGAAA GAACTAAATC 
     2461 TAATTCTGCA GAGACCATCA CAGAATTTTC ATCAGAATTC AGAGACTTCA AAAAAAGGCT 
     2521 TCATAGAGAG AGTGACAGCT GAACTATCAA GGTCCATCTA TCAGCTAATC GACGTGTACT 
     2581 GCAGTAGCTT CTGTACAGAT TTCCAGCCTG TGCACACACC CGGAGGTGTC TCCCATGTCC 
     2641 ACGCTGGGCT CCAGTCCCAC CTGAGCTTCA CAGTGTGTTC CCTGCACAAT GTCCCAGAGA 
     2701 CTTGGGCACA CAGCTACAAA GCATTTTCGT TTTCCTGCTG GCTCACATAT GCTGGGAAGA 
     2761 AGCTGTGCCA AGTGAAAAGC TGCAGACCCC TGCCAGTCAC AAAGTCATTC TCCCTTTTGG 
     2821 TCAACTGGAA TGAAATAATC AATTTTCCTC TTGAGATAAA GTCACTTCCA AGAGAATCCA 
     2881 TGCTCGTTAT AAAACTGTTC GGGATTGACT CTGCCACCCA CAGCACAAAT CTGCTGGCCT 
     2941 GGACCTGCCT TCCACTGTTT CCGAGACAGG AGTCTGTGCT GGGGTCTAGG CTGTTCAGTG 
     3001 TGACGTTACA GAGTGAGCCT CCAATCGAAA TGATAGCTCC AGGCGTATGG GATGGGAGCC 
     3061 AGCCTTCCCC ACTGACCCTG CAGATAGATT TTCCAGACGC TGGGTGGGAG TATCTGAAAC 
     3121 CTGAGTCTGA AGAGAACAGA ACTGACCATG AAGAGCCGCC AAGAGAGTGT TTAAAACACA 
     3181 TCGCCAAACT CTCCCAGAAG AAGTCTCCCT TGCTACTTTC TGAGGAAAAG AGGAGATATT 
     3241 TGTGGTTTTA TCGTCTCTAC TGCAACAATG AGAACTCCTC CCTCCCTTTG GTCTTGGGCA 
     3301 GCGCCCCTGG TTGGGATGAA GAGACAGTTT CTGAAATGCA TGCAATCTTG AGAAGGTGGA 
     3361 CGTTTTCACA CCCATGGGAA GCTCTTGGTC TTTTGACTTC CAGGTTTCCA GACCAAGACA 
     3421 TTCGTGAAGT TGCCGTTCAA CAATTAGACA CCCTCTTGAC TGACGAGCTG CTGGACTGCC 
     3481 TCCCACAGCT AGTGCAGGCT GTCAAGTTTG AGTGGAACCT CGAAAGTCCC TTGGTGGAAC 
     3541 TCCTGCCTCG TCGCCCCTTG CAAAGCATCC GAGTGGCTCA CTGTCTGTAC TGGCTGCTAC 
     3601 GGGATGCCCA AGGTGAAGCC TACTTTAAAA GCTGGTATCA GGAGCTGTTG GCCGCTCTCC 
     3661 AGTTCTGTGC AGGAGAAGCC CTGAATGAAG AGCTTTCCAA GGAGCAGAAA CTTGTCAAAC 
     3721 TTCTGGGTGA TATTGGAGAA AAAGTGAAGT CAGCCAGTGA CCCTCAGAGA AAGGATGTGC 
     3781 TAAAGAAAGA GATTGGCAGT CTAGAAGAAT TCTTTAAAGA TATAAAGACT TGCCATCTTC 
     3841 CTCTGAACCC GGCACTGTGC ATAAAAGGAA TTGATCGGGA TGCTTGCTCG TATTTCACAT 
     3901 CCAACGCTTC ACCATTGAAG ATTACTTTCA TCAATGCTAA TCCAATGGGC AAAAATATCA 
     3961 GTGTTATTTT TAAGGCCGGA GATGATCTTC GGCAGGATAT GCTTGCTCTG CAGATTATTC 
     4021 AAGTGATGGA CAACGCTTGG CTGCAGGAGG GCCTCGATAT GCAAATGATC ACTTATGGAT 
     4081 GTCTATCCAC AGGAAGAGCC CAAGGATTCA TAGAGATGGT GCCTGATGCT GTAACCCTGG 
     4141 CCAAGATCCA TCTGCACTCT GGGCTGATCG GACCCCTGAA AGAAAACACC ATCAAGAAGT 
     4201 GGTTCAGTCA GCACAACCAC TTAAAGGAAG ATTATGAAAA GGCCTTGAGG AACTTCTTTT 
     4261 ACTCTTGTGC TGGCTGGTGT GTGGTGACAT TCATCTTGGG AGTCTGTGAC CGACATAATG 
     4321 ACAATATCAT GCTGACAAAG TCAGGTCACA TGTTTCATAT TGACTTTGGA AAATTCTTGG 
     4381 GTCATGCGCA AACATTTGGC GGTATCAAAA GGGACAGAGC CCCTTTCATT TTTACTTCAG 
     4441 AGATGGAGTA CTTTATTACT GAGGGTGGAA AAAACATACA GCATTTTCAA GACTTCGTGG 
     4501 AGCTTTGCTG CAGAGCTTAC AACATCGTCA GGAAACACAG CCAGTTGATC CTGAGCCTTC 
     4561 TGGAGATGAT GCTGCATGCA GGACTGCCTG AGCTAAGGGG AATCGAAGAC CTGAAGTATG 
     4621 TACATAACAA TCTCCGCCCA CAAGACACAG ACCTGGAAGC CACAAGTCAT TTCACCAAGA 
     4681 AGATAAAGGA AAGTCTGGAG TGTTTCCCAG TGAAATTGAA TAACCTGATC CACACACTTG 
     4741 CACAGATGCC AGCCTTAAGC CTTGCCAAAC CTGCCCCTCA GACTCTTCTC CAGGAATCCT 
     4801 GCATCCTGAA TAAGACCAGG ACAATTCAGA GAGTCACAAT TTTAGGGTTC AGCAAGACAC 
     4861 ACAGCAACCT GTACCTGATG GAGGTGACAT GCAGTGACAA TAGGAGAAGC CTGACCAAGA 
     4921 AGTCCTTCGA GCAGTTTTAC AGACTTCACA GCCAAATGCA GAAGCAGTTC TCCTCGTTGG 
     4981 CTCTCCCAGA ATTTCCTCAC TGGTGGCATC TACCTTTCAC AGACTCAGAT CACAAGAGAA 
     5041 TCAGAGATCT GAGTCACTAC GTGGAACAGG TGCTACGCGG ATCTTATGAA GTTGCAAACA 
     5101 GTGATTGTGT ACTTAGCTTT TTTCTCTCTG AACATATTCA ACCAACACTT GAAGACTCTC 
     5161 CATTTGTGGA CCCAGGTGAG AACTCTCTAG ACAAGAGCCC TAAGGTGCAG TTACTGATGA 
     5221 CGTATGAGGA CTCGAGGCTC ACCATCCTAG TGAAACACTT GAAAAACATC CATCTCCCAG 
     5281 ATGGCTCTGT GCCCAGTGCA CATGTTGAAA TTTATCTTCT ACCACATCCC AGTGAAGTTC 
     5341 GGAGGAAGAA AACAAAGTGT GTTCCAAAGT GCACTGACCC AACTTACAAT GAAATTGTGG 
     5401 TATATGATGA AGTCTTGGGG CTCCAGGGCC ATGTTTTAAT GCTTATCGTG AAGAGCAAAA 
     5461 CTGTATTTGT GGGAGCAGTT AACATCCAGC TCTGCAGTGT ACCCCTCAAT GAAGAAAAGT 
     5521 GGTACCCACT AGGAAACAGT ATCATCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5581 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5641 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5701 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5761 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5821 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5881 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5941 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     6001 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     6061 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     6121 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6181 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6241 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6301 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6361 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6421 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6481 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6541 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6601 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6661 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6721 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6781 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6841 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6901 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6961 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     7021 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     7081 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     7141 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7201 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7261 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7321 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7381 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7441 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7501 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7561 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7621 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7681 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7741 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7801 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7861 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7921 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7981 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     8041 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     8101 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     8161 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8221 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8281 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8341 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8401 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8461 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8521 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8581 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8641 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8701 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8761 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8821 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8881 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8941 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     9001 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     9061 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     9121 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9181 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9241 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9301 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9361 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//