LOCUS       MR219379                8559 bp    DNA     circular  SYN 
DEFINITION  Aff1 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 1
ACCESSION   NM_001080798
VERSION     NM_001080798.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4716..4807 
                     /label="Myc-DDK"  
     misc_feature    4837..5422 
                     /label="PolyA signal"  
     misc_feature    4894..4913 
                     /label="Restriction Enzyme"  
     misc_feature    5698..6370 
                     /label="ColE1"  
     misc_feature    6800..7594 
                     /label="Kan/Neo"  
     misc_feature    7629..7931 
                     /label="SV40 ori"  
     misc_feature    8017..8494 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4698..4704 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4698 
                     /label="Aff1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAACGAGG ATTCTCCCAC GAGAGAGTGG TGGAGCCTGC TTGTACAATG 
     1081 AAGATAGAAA CCTGCTTCGA ATCAGAGAGA AGGAAAGACG CAACCAAGAA GCTCACCAGG 
     1141 AGAAGGAGGC ATTTCCCGAG AAGGCTCCCC TGTTTCCAGA GCCTTACAAG ACTGCAAAAG 
     1201 GCGATGAGCT ATCAAGTCGG ATCCAGACCA TGCTGGGTGA CTATGAGGAG ATGAAGGAGT 
     1261 TCCTGAGCAG CAAGTCCCAC CCCCACCGCC TGGATGGCTC TGAAGACAGG CCCGGGAAGC 
     1321 CCAGATATCC CTTAGGTCAT GACAGGGGGA ACGGGGCTGC ATCCAGCTCC CTCCGCACAC 
     1381 ATGTCTACCA CCAGCCTATC CACACTTCTG CTCCCGGATC ACGTCCTGTC GGTAACATTA 
     1441 GCCACAGTCC AAAGATGGCA CAGCCAAGGA TGGAGCCAAG TCTCCACACC AAAATCTATG 
     1501 ATGGCCCACG TCTGACTCAA GACCACCTCA GTCAGGGACA TTGTTCCAGA AAGTGTGACC 
     1561 GAAGAGCTGA AGGAGACTCT GCTCCCGAGA GGAAGCTCTC GCCCTTGATC TCCTCTTTGC 
     1621 CATCCCCGGT GCCCCCTCTA TCACCTGTAC ATTCCAGGCT GCAGGGAACC AGCAAGGCTC 
     1681 ACAGCAGTGG CGTTAGCAGT AAAAGCTGCT GTGTGGCCAA GTCTTCCAAG GACTTGGTGG 
     1741 CGAAGGCCCA AGATAAAGAG ACTCCTCATG ACGGTTTGGT GGCAGTTACC AGCCTTGGAT 
     1801 CAGCCCCTCC TCAGCCACCT TGCCAGACAT TTCCACCCCC TCCTCTCCCC TCAAAAAGTG 
     1861 CTGCAATGCA GCAGAAGCCC ACGGCATATG TCCGTCCCAT GGACGGTCAG GATCAGGCTC 
     1921 CCAGTGAGTC CCCCGAGCTG AAGCTGCCAC TGGAGGACTA TGGTCAGCAA AGCTTTGAGA 
     1981 AACCAGACCT TAAAGTGCCT GCCAAAGCCA AGCTCACCAG ACTAAGGATG CCCTCTCAGT 
     2041 CGGTGGAGCA GCCGTACTCC AATGAAGTCC ATTGCGTTGA AGAGATTCTG AAGGAAATGA 
     2101 CCCACTCGTG GCCACCGCCT CTGACAGCCA TACACACACC CAGTACAGCC GAGCCGTCCA 
     2161 GATTTCCTTT CCCCACAAAG GACCCTCTGC ACGTCAGTCC TGCAACCCAG AGCCAAAAAC 
     2221 AATATGACAC GCCTTCAAAA ACCCACCCCA ATCCCCAGCA AGGAACCTCC ATGCTGGAGG 
     2281 ACGACCTGCA GCTCAGCGAC AGTGAAGACA GCGACACGGA GCAAGCCACA GAGAAGCCTC 
     2341 CATCCCCACC TGCACCTCCC AGCGCTCCGC AGACACTTCC CGAGCCCGTG GCCTCAGCAC 
     2401 ATTCCAGTAG TGGGGAGTCA GAGAGCAGCG AGTCAGACAG TTCCTCAGAC TCTGAGAGTG 
     2461 AGAGCAGCTC CAGCGACAGC GAGGAGGAGG AAGAGAACGA GCCCCTGGAG ACCCGGGCTC 
     2521 CCGAGCCCGA GCCTCCGACG ACAAACAAAT GGCAGCTGGA CAACTGGTTG ACCAAAGTCA 
     2581 ACCAACCCTC AGTGCCCCTG GATGGCCGGG GGAGCACAGA GTCTCCACAA TGGCGCCAGG 
     2641 AAAGTAAGGG TGTCGCTGAG GGCAGCAGTG ACCAGCAGCA TCCTGATTCC AAAGACCCTC 
     2701 TCCCAAAGAG CTCCAGCAAG ACTCTCCGGG GTCCCTCTGA GGGGCCCCAG CCTGGGAAGA 
     2761 GGGGCTGTCC GAAATCCCCC GCTCAACAGG AACCCCCACC TCGGCAAACC GTGGGCAGCA 
     2821 AACAACCCAG GAAACCCGCC AAGGGCTCCG GCCAGGCAGA GCCCCAGGCC AGCTCGCAGG 
     2881 CAGAGAGTGA AGTAGGACCC CTTCCCTACG GCTCAAAGGA GCAGACTTCC AAAGACAGAC 
     2941 CCAAGGTGAA GACGAAAGGC AGGCCGCGTG CCGTGGGCAG TCGGGAGCCT AAGCCAGAGG 
     3001 TCCCTGCGCC CACGCCTCAG GCAGCCGTGC CCAGACCGAA GCCGCCTGTG CCCACCCCCA 
     3061 GTGAGAAGAG GAAGCACAAG AGCTCCACGG CCCCCTCCAA GGCGCCCTCA GCCCCCCAGC 
     3121 CTCCGAAGGA CAGTGCTGGG GACAGGAACC CAGAACACTC TGCTCTTGTC TCCTTGACTC 
     3181 AGAGCCAGGG TCCATCCCAC AGCAGCAGGG GCAGCAGTGG CAGCGTCAGG ACTAGTGGCT 
     3241 GCCGACAGGC TGTGATTGCC CAAGGGGACG GCTGTAAAGA CAAGCTCCTG TTGCCTTTGA 
     3301 GAGACACCAA ATTGCTCTCG CCGCTCAGGG ACTCCCCACC ACCGACAAGC TTGGTGGTGA 
     3361 AGATAACCCT AGACCTCCTC ACGAGGATTC CCCAGCCTCT GGGGAAGGGG AGCCGCCCCA 
     3421 GGAAAGCAGA AGACAAGCAG CTGTCAGCAG GGAAGAAGCA GGACTCGGAG ACCAAGAGCT 
     3481 GTGACAGCTC CAGCAGAGTG ACCAAGAAGA GAAAGGGTGA CCCAGAAAAA GAGCACAGTA 
     3541 ACAAGAGACA CAAACTGGAT AAGTCGCAGA CTGCGTCATC TTCATCTTCC CATACAGAAT 
     3601 CTTCCAGAAC AAAAGCGCCC AGGTCCTCCT CAGAGAACTC AAGAAAGGAA ATGCTGCCTC 
     3661 CAGCATCGGC GTCCTCAGTC TCCTCTTCCT CATCCTCCCA GAAGCCAAGC AGACCTGCAC 
     3721 AGAAGAGGCC AAGGCCAGAC GAAGACACGT GTAGCCAGGA ACCCCCCAGA AGTGCCAGCA 
     3781 GCACAAAGAG CAGCTCTACA GACCCTCCCG CTCCCAAGCA CAGAAAAGTA CAGGCCAGGG 
     3841 GCTCTGAACA CAAGGGATCT TCTGGGGATG CTGCGAATGC TGCAAATCCT TTTCCAGTGC 
     3901 CTTCTTTGCC GAATGGTAAC GCTAAACCAG GGAAGCCACA GGTGAAGTCT GACAGACAAC 
     3961 AAGCTGACTT TCACATGAAG GAGGCTAAAA AGCTGAAGTG CAAAGCAGAG ACAATGGTAG 
     4021 ACAAGGCTGG AAAAGCCTTC AAGTATTTGG AAGCCGTCCT CTCTTTTATC GAGTGTGGAA 
     4081 TGGCCTCAGA GTCAGAAAGC TCAGCCAAGT CGGCGTACGC TGTGTACTCA GAAACCATAG 
     4141 ACCTCATTAG GTATGTGATG TCACTAAAAT GCTTCTCAGA TAACACGATG CCAGCACAAG 
     4201 AGAAGATATT TGCTGTTTTA TGCTTGCGTT GCCAGTCCCT TTTGAACATG GCGATGTTTC 
     4261 GATGTAAGAA AGACACAGTG ATGAAGTATT CTCGTACTCT CAGTGAACAC TTCAAGAGTA 
     4321 CTTCCAAAGT GGCCCAGGCA CCTTCTCCAT GCACGGCAAG AAGCACAGGC GTTCCGTCCC 
     4381 CCCTCTCCCC AATGCCTTCT CCTGCCAGCT CCGTGGGGTC CCAATCAAGT GCTGGCAGCA 
     4441 GTATGGGGAG CGTCGGGGTG ACGGCCACCG TCAGTACTCC AGTCTCCATC CAGAACATGA 
     4501 CCTCCTCCTA TGTCACCATC ACATCCCATG TCCTTACGGC CTTTAGCCTT TGGGAACAGG 
     4561 CCGAGGCCCT CACAAGGAAG AACAAAGAAT TCTTCGCTCA GCTCAGCACA AAAGTGCGTG 
     4621 TGTTGGCCCT CAACAGCAGC CTGGTGGACC TGGTGCACTA CACAAGACAG GGCCTGCAGC 
     4681 GGCTAAAACA GTCACCCAAA GGCCCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4741 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4801 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4861 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4921 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4981 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5041 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5101 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5161 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5221 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5281 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5341 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5401 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5461 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5521 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5581 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5641 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5701 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5761 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5821 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5881 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5941 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6001 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6061 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6121 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6181 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6241 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6301 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6361 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6421 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6481 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6541 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6601 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6661 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6721 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6781 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6841 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6901 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6961 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7021 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7081 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7141 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7201 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7261 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7321 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7381 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7441 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7501 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7561 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7621 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7681 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7741 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7801 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7921 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7981 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8041 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8161 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8221 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8281 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8341 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8401 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8461 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8521 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//