LOCUS       MR219363                7848 bp    DNA     circular  SYN 
DEFINITION  Kcnh1 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 1 (Kcnh1), transcript variant 1
ACCESSION   NM_010600
VERSION     NM_010600.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4005..4096 
                     /label="Myc-DDK"  
     misc_feature    4126..4711 
                     /label="PolyA signal"  
     misc_feature    4183..4202 
                     /label="Restriction Enzyme"  
     misc_feature    4987..5659 
                     /label="ColE1"  
     misc_feature    6089..6883 
                     /label="Kan/Neo"  
     misc_feature    6918..7220 
                     /label="SV40 ori"  
     misc_feature    7306..7783 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3987..3993 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3987 
                     /label="Kcnh1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCATGGCT GGCGGCCGGC GGGGACTAGT GGCCCCGCAG AACACGTTTC 
     1081 TGGAGAACAT CGTGCGGCGG TCCAACGACA CTAATTTTGT GTTGGGGAAT GCCCAGATAG 
     1141 TCGACTGGCC CATCGTGTAC AGCAATGATG GATTCTGCAA GCTGTCTGGC TACCACCGGG 
     1201 CAGAAGTGAT GCAGAAAAGC AGCGCCTGCA GTTTTATGTA TGGAGAGCTG ACTGACAAGG 
     1261 ACACAGTTGA AAAGGTTCGC CAGACCTTTG AGAACTACGA GATGAATTCC TTCGAAATTC 
     1321 TGATGTACAA GAAGAACAGG ACACCTGTGT GGTTTTTTGT GAAGATCGCT CCGATCAGGA 
     1381 ACGAACAGGA TAAAGTGGTC CTGTTCCTTT GCACTTTCAG CGACATAACT GCATTCAAGC 
     1441 AGCCCATCGA GGACGACTCC TGCAAAGGTT GGGGGAAGTT TGCCCGACTG ACCAGAGCTC 
     1501 TGACAAGCAG CAGGGGAGTC CTGCAGCAGC TGGCCCCCAG TGTACAGAAG GGTGAGAATG 
     1561 TTCACAAGCA CTCGCGCCTG GCAGAGGTCC TGCAGCTGGG TTCAGACATC CTTCCCCAGT 
     1621 ACAAGCAAGA GGCGCCAAAG ACACCCCCTC ACATCATCCT ACACTACTGT GTCTTTAAGA 
     1681 CCACGTGGGA TTGGATCATC TTGATCCTGA CCTTCTACAC AGCCATCTTG GTCCCTTACA 
     1741 ACGTCTCCTT TAAAACCAGG CAGAATAATG TGGCCTGGCT GGTGGTGGAC AGCATCGTGG 
     1801 ATGTCATCTT TTTGGTGGAC ATTGTTTTGA ATTTTCACAC CACCTTTGTC GGGCCAGCGG 
     1861 GGGAAGTGAT ATCTGACCCC AAACTTATCC GCATGAACTA CCTGAAGACG TGGTTTGTGA 
     1921 TCGACCTTCT GTCCTGTTTG CCGTATGACG TCATCAACGC TTTTGAGAAC GTGGATGAGG 
     1981 TTAGTGCCTT TATGGGTGAT CCAGGGAAGA TTGGTTTTGC TGATCAGATC CCACCACCAC 
     2041 TGGAGGGGAG AGAGAGTCAG GGCATCAGCA GCCTGTTCAG TTCTCTGAAA GTTGTGCGGC 
     2101 TGCTCCGTCT CGGGCGAGTG GCCCGCAAAC TGGATCATTA CATCGAGTAT GGAGCCGCGG 
     2161 TACTGGTCCT GTTGGTGTGC GTGTTTGGGC TTGCTGCCCA CTGGATGGCC TGCATCTGGT 
     2221 ACAGCATTGG GGACTATGAG ATCTTTGATG AAGACACCAA GACCATCCGC AACAACAGCT 
     2281 GGCTCTACCA ACTGGCATTG GACATCGGCA CTCCATACCA GTTTAATGGG TCTGGTTCAG 
     2341 GGAAGTGGGA AGGTGGGCCA AGCAAGAACT CTGTCTACAT CTCCTCGCTG TACTTCACCA 
     2401 TGACAAGTCT CACCAGTGTG GGCTTTGGTA ACATTGCCCC ATCCACAGAC ATCGAGAAGA 
     2461 TCTTCGCAGT GGCCATCATG ATGATTGGCT CTCTTCTGTA TGCCACCATC TTTGGGAACG 
     2521 TGACAACCAT TTTCCAGCAG ATGTACGCCA ACACCAACAG GTACCACGAG ATGCTCAACA 
     2581 GTGTCCGGGA TTTCCTGAAG CTCTACCAGG TGCCCAAAGG GCTGAGTGAG CGGGTCATGG 
     2641 ACTACATTGT GTCCACCTGG TCCATGTCCC GAGGCATCGA CACAGAGAAG GTCCTGCAGA 
     2701 TCTGCCCCAA GGACATGCGA GCCGACATTT GCGTGCACCT GAACCGCAAA GTGTTCAAAG 
     2761 AACACCCTGC CTTTCGATTG GCCAGCGATG GCTGCCTGCG GGCCTTGGCC ATGGAGTTCC 
     2821 AGACAGTGCA CTGTGCCCCA GGGGACCTCA TCTATCATGC TGGAGAGAGT GTGGACAGCC 
     2881 TCTGCTTCGT GGTCTCAGGC TCCCTGGAGG TGATCCAGGA TGACGAGGTG GTGGCCATCC 
     2941 TAGGGAAAGG AGACGTGTTT GGGGATGTTT TCTGGAAGGA AGCTACCCTT GCACAGTCCT 
     3001 GTGCTAATGT CCGGGCCTTG ACCTACTGTG ACCTGCACGT GATCAAGAGG GACGCCCTTC 
     3061 AGAAAGTGCT AGAATTCTAC ACAGCCTTCT CCCACTCCTT CTCCCGGAAC CTGATTCTCA 
     3121 CCTACAACCT GAGGAAGAGG ATTGTGTTCC GGAAGATCAG CGACGTGAAA CGAGAAGAGG 
     3181 AGGAGAGGAT GAAACGGAAG AACGAGGCCC CCCTTATCCT GCCTCCTGAC CACCCCGTCA 
     3241 GAAGACTCTT CCAAAGGTTC CGCCAGCAGA AAGAAGCCAG GCTGGCAGCC GAGAGAGGGG 
     3301 GCCGGGACCT GGATGACCTG GATGTAGAAA AGGGCAATGC CCTCACGGAC CATACCTCAG 
     3361 CCAACCACAG CCTGGTGAAG GCCAGTGTGG TCACGGTGCG TGAGAGCCCC GCCACGCCTG 
     3421 TGTCCTTCCA GGCGGCCACC ACCTCCACCA TGTCAGACCA CGCCAAGCTG CATGCCCCCG 
     3481 GATCTGAGTG CCTAGGTCCC AAGGCAGTCA GCTGTGACCC TGCCAAGCGC AAAGGCTGGG 
     3541 CCCGTTTCAA AGATGCCTGT GGAAAGGGTG AGGATTGGAA CAAGGTGTCC AAGGCAGAGT 
     3601 CCATGGAGAC GCTGCCCGAG AGGACAAAGG CACCGGGTGA GGCCACGCTG AAGAAGACAG 
     3661 ACTCCTGTGA CAGCGGAATC ACCAAGAGCG ACCTGCGCTT GGACAATGTG GGTGAGACCA 
     3721 GGAGTCCTCA GGACCGGAGC CCCATCTTGG CTGAGGTCAA GCATTCTTTC TACCCCATCC 
     3781 CTGAGCAGAC GCTGCAGGCC ACAGTGCTGG AGGTGAAATA TGAGCTGAAG GAGGATATCA 
     3841 AGGCCTTGAA TGCCAAAATG ACCTCCATTG AGAAGCAGCT GTCTGAGATA CTCAGGATAC 
     3901 TAATGTCCAG AGGGTCCGCC CAATCTCCTC AGGAGACGGG TGAGATCTCC AGGCCCCAGT 
     3961 CCCCAGAGTC AGACAGAGAC ATTTTTGGAG CAAGCACGCG TACGCGGCCG CTCGAGCAGA 
     4021 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4081 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4141 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4201 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4261 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4321 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4381 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4441 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4501 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4561 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4621 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4681 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4741 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4801 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4861 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4921 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4981 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5041 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5101 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5161 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5221 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5281 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5341 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5401 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5461 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5521 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5581 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5641 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5701 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5761 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5821 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5881 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5941 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6001 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6061 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6121 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6181 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6241 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6301 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6361 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6421 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6481 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6541 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6601 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6661 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6721 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6781 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6841 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6901 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6961 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7021 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7081 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7141 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7201 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7261 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7321 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7381 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7441 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7501 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7561 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7621 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7681 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7741 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7801 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//