LOCUS       MR219347                8223 bp    DNA     circular  SYN 
DEFINITION  Tbc1d8b (Myc-DDK-tagged) - Mouse TBC1 domain family, member 8B (Tbc1d8b)
ACCESSION   NM_001081499
VERSION     NM_001081499.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4380..4471 
                     /label="Myc-DDK"  
     misc_feature    4501..5086 
                     /label="PolyA signal"  
     misc_feature    4558..4577 
                     /label="Restriction Enzyme"  
     misc_feature    5362..6034 
                     /label="ColE1"  
     misc_feature    6464..7258 
                     /label="Kan/Neo"  
     misc_feature    7293..7595 
                     /label="SV40 ori"  
     misc_feature    7681..8158 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4362..4368 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4362 
                     /label="Tbc1d8b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCTGAAG CCCGAGGAAG TGCTGTTGAA AAATGCGCTG AAGCTGTGGC 
     1081 TGATGGAAAG ATCCAACGAG TACTTTGTCC TGCAAAGGCG TCGAGGCTAC GGGGAAGAAG 
     1141 GAGGAGGGGG ACTTACAGGG CTCTTGGTTG GGACTCTTGA TTCTGTCTTA GACTCCACTG 
     1201 CTAAAGTAGC TCCATTTCGC ATCCTACACC AGACACCAGA TTCTCAAGTG TACTTGTCAA 
     1261 TTGCATGTGG AGCCAACAGA GAAGAAATAA CAAAACATTG GGACTGGTTG GAACAAAATA 
     1321 TAATGAAGAC TTTATCTGTG TTTGATTCAA ATGAAGATAT TACCAATTTT GTACAAGGCA 
     1381 AAATAAGAGG ACTAATCGCT GAAGAGGGGA AACAGTCTTT TGCTAAAGAA GATGATCCTG 
     1441 AGAAATTTCG AGAAGCCCTT TTGAAATTTG AAAAATCTTT TGGTTTACCA GAGCAGGAAA 
     1501 AATTAGTAAC TTATTATTCC TGCAGTTACT GGAGAGGACG GGTCCCTTGT CAGGGCTGGC 
     1561 TCTATCTAAG TACCAACTTT CTGAGCTTCT ATTCCTTCTT GCTAGGATCA GAAATTAAAC 
     1621 TCATTATCTC CTGGGATGCA ATCTCAAAAC TTGAAAAGAC TTCAACTGTT ATATTAACTG 
     1681 AGAGCATTCA CGTATGTTCT CAAGGAGAGA ATCACTATTT TTCAATGTTT TTGCACATTA 
     1741 ATGAGACATA CCTTCTTATG GAACAACTGG CAAACTATGC CATAAAGAGA CTTTTTGATA 
     1801 AAGAAACATT TGATAATGAC CCAGTCCTTG ATGATCCTCT ACAGATTACC AAAAGAGGTT 
     1861 TGGAATATCG AGCCCACAGT GAGCAATTCA AAGCCTTTTT TAGGCTACCC AAAGAAGAGA 
     1921 CATTGAAAGA AGTACATGAG TGTTTCTTAT GGGTACCATT CAGCCACTTC AGTAGTCACG 
     1981 GAAAAATGTG CATTTCAGAA AACTATATTT GCTTTGCTAG CCAGGATGGG AATCTATGCA 
     2041 GTGTAATCAT TCCATTACGA GAGGTCTTAG CTATAGATAA AACTGATGAC TCCAACAGAT 
     2101 CGGTCATCAT TAGCATCAAA GGAAAAACAG CTTTTCGTTT CAGTGAGCTT AAAGACTTTG 
     2161 AGCAACTGGT GGCAAAACTC AGGCTCAAGT GCCGGGCAGC TTCAACTCAA GATGATGTTA 
     2221 GTACAGAGGT TGCTGTTAGT TCTGACTCGA CAGGCCCATC TGAGAATTTT GAGGAGCAGC 
     2281 CTTTGACATG TCCAAAGGAA TGCAGCAAAA CTGTGAACAC TGAAGCCTTA ATGACAGTAT 
     2341 TTCACCCTCA AAATCTGGAA AATCTTGATT CTAAAATGTT GAAAGAAAAG ATGAAGGAGC 
     2401 AATCATGGAA CATCTTATTT TCAGAATGTG GGCGAGGTGT TAGCATGTTC CGGACAAAAA 
     2461 AGACTCGAGA TCTGGTGGTA AGAGGGATTC CAGAGACGTT AAGAGGAGAG CTCTGGATGC 
     2521 TTTTCTCAGG TGCTGTTAAT GACATGGCAA CAAACCCTGG CTATTATGCT GAAGTGGTTG 
     2581 AGCAATCCTT AGGGACCTCC AACTTGGCTA CTGAAGAAAT TGAGCGTGAT TTGCGTCGCT 
     2641 CTCTGCCCGA ACATCCAGCA TTTCAGAGTG ATACTGGCAT ATCTGCTCTG AGGCGGGTAC 
     2701 TGACAGCTTA TGCATATAGG AATCCCAAAA TAGGATACTG CCAGGCAATG AACATTTTGA 
     2761 CTTCAGTATT GCTCCTATAT GCAAAAGAAG AAGAGGCTTT TTGGCTTCTG GTTGCTGTAT 
     2821 GTGAACGAAT GTTGCCTGAT TATTTTAATC GTCGAATCAT TGGTGCCTTG GTGGACCAGG 
     2881 CAGTGTTTGA AGAACTTATC AGGGATCACC TTCCTCAGCT GACAGACCAC ATGACCGACA 
     2941 TGACTTTCTT TTCCTCTGTT TCTCTTTCCT GGTTTCTCAC ACTTTTCATT AGTGTGCTAC 
     3001 CTATTGAAAG TGCAGTGAAT GTGGTGGACT GTTTCTTCTA TGATGGAATA AAAGCCATTT 
     3061 TACAACTGGG ATTGGCAATA CTTGACTATA ATTTAGACAA ACTTCTGACA TGTAAAGATG 
     3121 ATGCTGAAGC GGTGACGGCA CTAAACAGGT TCTTTGACAA TGTCATTAAT AAGGATAGCC 
     3181 CACTGCCTTC AAATGTTCAA CAGGGTTCAA ATATCAGTAA TGAAAAAAGT GATCATACTA 
     3241 AAGTGGATAT TACAGATTTG ATTAAAGAGT CCAATGAGAA ATATGGTAGT ATCCGCTATG 
     3301 AAGATATACA TAGCATGCGC TGTCGAAATA GGTTGTATGT GATCCAGACC CTGGAAGAGA 
     3361 CAACAAAGCA GAATGTGCTA CGTGTTGTAT CGCAAGACGT GAAAATGAGC CTTCAGGAAC 
     3421 TGGACGAACT TTATGTCATT TTTAAGAAAG AGCTGTTTAT ATCTTGTTAT TGGTATTTGA 
     3481 GTTGTCCAGG ATTGAAGCAT CATGACCCCA GCCTGCCATA TTTGGAACAA TATCAGATTG 
     3541 ACTGCCAGCA ATTCAGAGTA TTATATCACT TGCTGAGTCC CTGGGCTCAT TCTGCAAACA 
     3601 GAGACTCATT GGCTTTATGG ACATTCCGAT TGTTGGATGA GAACTCTGAT TGCCTTATAA 
     3661 ACTTCAAAGA ATTCTCCTCT GCAATAGATA TAATGTACAA TGGAAGTTTT ACTGACAAGC 
     3721 TCAAGCTGCT TTTTAAGCTG CATATTCCTC CAGCTTATAC TGAAGTGATG TCTAAGACGT 
     3781 CTTCAAAGGG CGATGAGCTT TCAACAGAAG AATTACTTTA TTTCAGTCAG CTCCAGGTTT 
     3841 CTAAGCCTGC AGATGAGAAG GAAACAGAAT CGGGAAGAAA TAGCCCTGAA AAAGGCAAAG 
     3901 GAAAAATTGA TATTCAAGCC TATTTAAGTC AATGGCAAGA TGAGCTTCTT AAAAAAGAAG 
     3961 AAACCATTAA AGATTTACCA AGAATGAATC AGTCACAATT TATTCAATTT TCAAAGACCC 
     4021 TGTATAATTT ATTTCATGAG GACCCTGAAG AAGAGTCGCT ATATCAAGCC ATTGCTATTG 
     4081 TAACCAACCT TCTGCTCAGG ATGGAAGAAG TTGGAAGGAA ACTCCATAGC CCGGCATCAT 
     4141 CAGCCAGTAC AGCTCGTGAT TCTGGACCCA GTGAAGGGAA TGCAGAGAGC AGTGTGAAGA 
     4201 AAGATCTTCC CTCTCCCAGG GAAGAACATC AGTGGTCGTT TGCATTTGAG CAGATTCTTG 
     4261 CTTCCCTGTT AAATGAACCA GCCTTGGTGA GGTTTTTTGA GAGACCCTTA GATTTAAAAG 
     4321 CCAAGCTAGA AAATGCGAAA AGCTCCCAGC TAAGGTCTAG AACCAAGATG ACGCGTACGC 
     4381 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4441 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4501 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4561 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4621 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4681 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4741 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4801 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4861 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4921 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4981 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5041 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5101 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5161 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5221 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5281 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5341 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5401 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5461 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5521 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5581 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5641 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5701 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5761 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5821 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5881 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5941 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6001 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6061 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6121 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6181 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6241 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6301 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6361 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6421 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6481 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6541 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6601 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6661 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6721 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6781 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6841 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6901 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6961 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7021 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7081 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7141 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7201 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7261 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7321 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7381 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7441 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7561 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7621 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7681 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7741 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7801 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7861 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7921 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7981 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8041 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8101 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8161 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8221 TTT
//