LOCUS       MR219346                8535 bp    DNA     circular  SYN 
DEFINITION  Aff1 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 2
ACCESSION   NM_133919
VERSION     NM_133919.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4692..4783 
                     /label="Myc-DDK"  
     misc_feature    4813..5398 
                     /label="PolyA signal"  
     misc_feature    4870..4889 
                     /label="Restriction Enzyme"  
     misc_feature    5674..6346 
                     /label="ColE1"  
     misc_feature    6776..7570 
                     /label="Kan/Neo"  
     misc_feature    7605..7907 
                     /label="SV40 ori"  
     misc_feature    7993..8470 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4674..4680 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4674 
                     /label="Aff1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCCAT TCCAGCTTGT ACAATGAAGA TAGAAACCTG CTTCGAATCA 
     1081 GAGAGAAGGA AAGACGCAAC CAAGAAGCTC ACCAGGAGAA GGAGGCATTT CCCGAGAAGG 
     1141 CTCCCCTGTT TCCAGAGCCT TACAAGACTG CAAAAGGCGA TGAGCTATCA AGTCGGATCC 
     1201 AGACCATGCT GGGTGACTAT GAGGAGATGA AGGAGTTCCT GAGCAGCAAG TCCCACCCCC 
     1261 ACCGCCTGGA TGGCTCTGAA GACAGGCCCG GGAAGCCCAG ATATCCCTTA GGTCATGACA 
     1321 GGGGGAACGG GGCTGCATCC AGCTCCCTCC GCACACATGT CTACCACCAG CCTATCCACA 
     1381 CTTCTGCTCC CGGATCACGT CCTGTCGGTA ACATTAGCCA CAGTCCAAAG ATGGCACAGC 
     1441 CAAGGATGGA GCCAAGTCTC CACACCAAAA TCTATGATGG CCCACGTCTG ACTCAAGACC 
     1501 ACCTCAGTCA GGGACATTGT TCCAGAAAGT GTGACCGAAG AGCTGAAGGA GACTCTGCTC 
     1561 CCGAGAGGAA GCTCTCGCCC TTGATCTCCT CTTTGCCATC CCCGGTGCCC CCTCTATCAC 
     1621 CTGTACATTC CAGGCTGCAG GGAACCAGCA AGGCTCACAG CAGTGGCGTT AGCAGTAAAA 
     1681 GCTGCTGTGT GGCCAAGTCT TCCAAGGACT TGGTGGCGAA GGCCCAAGAT AAAGAGACTC 
     1741 CTCATGACGG TTTGGTGGCA GTTACCAGCC TTGGATCAGC CCCTCCTCAG CCACCTTGCC 
     1801 AGACATTTCC ACCCCCTCCT CTCCCCTCAA AAAGTGCTGC AATGCAGCAG AAGCCCACGG 
     1861 CATATGTCCG TCCCATGGAC GGTCAGGATC AGGCTCCCAG TGAGTCCCCC GAGCTGAAGC 
     1921 TGCCACTGGA GGACTATGGT CAGCAAAGCT TTGAGAAACC AGACCTTAAA GTGCCTGCCA 
     1981 AAGCCAAGCT CACCAGACTA AGGATGCCCT CTCAGTCGGT GGAGCAGCCG TACTCCAATG 
     2041 AAGTCCATTG CGTTGAAGAG ATTCTGAAGG AAATGACCCA CTCGTGGCCA CCGCCTCTGA 
     2101 CAGCCATACA CACACCCAGT ACAGCCGAGC CGTCCAGATT TCCTTTCCCC ACAAAGGACC 
     2161 CTCTGCACGT CAGTCCTGCA ACCCAGAGCC AAAAACAATA TGACACGCCT TCAAAAACCC 
     2221 ACCCCAATCC CCAGCAAGGA ACCTCCATGC TGGAGGACGA CCTGCAGCTC AGCGACAGTG 
     2281 AAGACAGCGA CACGGAGCAA GCCACAGAGA AGCCTCCATC CCCACCTGCA CCTCCCAGCG 
     2341 CTCCGCAGAC ACTTCCCGAG CCCGTGGCCT CAGCACATTC CAGTAGTGGG GAGTCAGAGA 
     2401 GCAGCGAGTC AGACAGTTCC TCAGACTCTG AGAGTGAGAG CAGCTCCAGC GACAGCGAGG 
     2461 AGGAGGAAGA GAACGAGCCC CTGGAGACCC GGGCTCCCGA GCCCGAGCCT CCGACGACAA 
     2521 ACAAATGGCA GCTGGACAAC TGGTTGACCA AAGTCAACCA ACCCTCAGTG CCCCTGGATG 
     2581 GCCGGGGGAG CACAGAGTCT CCACAATGGC GCCAGGAAAG TAAGGGTGTC GCTGAGGGCA 
     2641 GCAGTGACCA GCAGCATCCT GATTCCAAAG ACCCTCTCCC AAAGAGCTCC AGCAAGACTC 
     2701 TCCGGGGTCC CTCTGAGGGG CCCCAGCCTG GGAAGAGGGG CTGTCCGAAA TCCCCCGCTC 
     2761 AACAGGAACC CCCACCTCGG CAAACCGTGG GCAGCAAACA ACCCAGGAAA CCCGCCAAGG 
     2821 GCTCCGGCCA GGCAGAGCCC CAGGCCAGCT CGCAGGCAGA GAGTGAAGTA GGACCCCTTC 
     2881 CCTACGGCTC AAAGGAGCAG ACTTCCAAAG ACAGACCCAA GGTGAAGACG AAAGGCAGGC 
     2941 CGCGTGCCGT GGGCAGTCGG GAGCCTAAGC CAGAGGTCCC TGCGCCCACG CCTCAGGCAG 
     3001 CCGTGCCCAG ACCGAAGCCG CCTGTGCCCA CCCCCAGTGA GAAGAGGAAG CACAAGAGCT 
     3061 CCACGGCCCC CTCCAAGGCG CCCTCAGCCC CCCAGCCTCC GAAGGACAGT GCTGGGGACA 
     3121 GGAACCCAGA ACACTCTGCT CTTGTCTCCT TGACTCAGAG CCAGGGTCCA TCCCACAGCA 
     3181 GCAGGGGCAG CAGTGGCAGC GTCAGGACTA GTGGCTGCCG ACAGGCTGTG ATTGCCCAAG 
     3241 GGGACGGCTG TAAAGACAAG CTCCTGTTGC CTTTGAGAGA CACCAAATTG CTCTCGCCGC 
     3301 TCAGGGACTC CCCACCACCG ACAAGCTTGG TGGTGAAGAT AACCCTAGAC CTCCTCACGA 
     3361 GGATTCCCCA GCCTCTGGGG AAGGGGAGCC GCCCCAGGAA AGCAGAAGAC AAGCAGCTGT 
     3421 CAGCAGGGAA GAAGCAGGAC TCGGAGACCA AGAGCTGTGA CAGCTCCAGC AGAGTGACCA 
     3481 AGAAGAGAAA GGGTGACCCA GAAAAAGAGC ACAGTAACAA GAGACACAAA CTGGATAAGT 
     3541 CGCAGACTGC GTCATCTTCA TCTTCCCATA CAGAATCTTC CAGAACAAAA GCGCCCAGGT 
     3601 CCTCCTCAGA GAACTCAAGA AAGGAAATGC TGCCTCCAGC ATCGGCGTCC TCAGTCTCCT 
     3661 CTTCCTCATC CTCCCAGAAG CCAAGCAGAC CTGCACAGAA GAGGCCAAGG CCAGACGAAG 
     3721 ACACGTGTAG CCAGGAACCC CCCAGAAGTG CCAGCAGCAC AAAGAGCAGC TCTACAGACC 
     3781 CTCCCGCTCC CAAGCACAGA AAAGTACAGG CCAGGGGCTC TGAACACAAG GGATCTTCTG 
     3841 GGGATGCTGC GAATGCTGCA AATCCTTTTC CAGTGCCTTC TTTGCCGAAT GGTAACGCTA 
     3901 AACCAGGGAA GCCACAGGTG AAGTCTGACA GACAACAAGC TGACTTTCAC ATGAAGGAGG 
     3961 CTAAAAAGCT GAAGTGCAAA GCAGAGACAA TGGTAGACAA GGCTGGAAAA GCCTTCAAGT 
     4021 ATTTGGAAGC CGTCCTCTCT TTTATCGAGT GTGGAATGGC CTCAGAGTCA GAAAGCTCAG 
     4081 CCAAGTCGGC GTACGCTGTG TACTCAGAAA CCATAGACCT CATTAGGTAT GTGATGTCAC 
     4141 TAAAATGCTT CTCAGATAAC ACGATGCCAG CACAAGAGAA GATATTTGCT GTTTTATGCT 
     4201 TGCGTTGCCA GTCCCTTTTG AACATGGCGA TGTTTCGATG TAAGAAAGAC ACAGTGATGA 
     4261 AGTATTCTCG TACTCTCAGT GAACACTTCA AGAGTACTTC CAAAGTGGCC CAGGCACCTT 
     4321 CTCCATGCAC GGCAAGAAGC ACAGGCGTTC CGTCCCCCCT CTCCCCAATG CCTTCTCCTG 
     4381 CCAGCTCCGT GGGGTCCCAA TCAAGTGCTG GCAGCAGTAT GGGGAGCGTC GGGGTGACGG 
     4441 CCACCGTCAG TACTCCAGTC TCCATCCAGA ACATGACCTC CTCCTATGTC ACCATCACAT 
     4501 CCCATGTCCT TACGGCCTTT AGCCTTTGGG AACAGGCCGA GGCCCTCACA AGGAAGAACA 
     4561 AAGAATTCTT CGCTCAGCTC AGCACAAAAG TGCGTGTGTT GGCCCTCAAC AGCAGCCTGG 
     4621 TGGACCTGGT GCACTACACA AGACAGGGCC TGCAGCGGCT AAAACAGTCA CCCAAAGGCC 
     4681 CCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4741 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4801 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4861 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4921 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4981 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5041 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5101 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5161 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5221 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5281 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5341 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5401 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5461 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5521 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5581 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5641 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5701 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5761 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5821 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5881 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5941 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6001 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6061 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6121 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6181 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6241 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6301 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6361 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6421 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6481 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6541 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6601 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6661 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6721 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6781 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6841 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6901 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6961 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7021 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7081 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7141 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7201 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7261 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7321 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7381 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7441 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7501 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7561 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7621 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7681 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7741 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7861 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7921 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7981 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8041 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8101 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8161 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8221 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8281 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8341 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8401 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8461 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8521 TTTAACGCGA ATTTT
//