LOCUS       MR219330                6606 bp    DNA     circular  SYN 
DEFINITION  Ces5a (Myc-DDK-tagged) - Mouse carboxylesterase 5A (Ces5a)
ACCESSION   NM_001003951
VERSION     NM_001003951.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2763..2854 
                     /label="Myc-DDK"  
     misc_feature    2884..3469 
                     /label="PolyA signal"  
     misc_feature    2941..2960 
                     /label="Restriction Enzyme"  
     misc_feature    3745..4417 
                     /label="ColE1"  
     misc_feature    4847..5641 
                     /label="Kan/Neo"  
     misc_feature    5676..5978 
                     /label="SV40 ori"  
     misc_feature    6064..6541 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2745..2751 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2745 
                     /label="Ces5a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGGGGAC TGGGTGCGTC CAGGACAAGC CCTAATCTGG GTTATTTGGA 
     1081 TCTTTGGAGC CATCATCGAG GGGTCTGTGA CTGAAGAGCC ACATAGGTAC ACCAAGCTGG 
     1141 GATGGGTGCA GGGGAAGCAA GCCACCGTGC TGGGAAGACT GGAACCCGTG AACGTGTTTC 
     1201 TTGGGATCCC CTTTGCTGCA CCCCCTCTTG GACCTCTGCG GTTTTCCAAG CCACAGCCTC 
     1261 CGATCCCCTG GGATAATTTG CGAGAAGCCA CTGCCTATCC CAATTTGTGC TTTCAGAACT 
     1321 TAGAGTGGTT ATTCATATAT CAAAACCTGC TCAAAGTAAG TTACCCTATA CTTGGAATGT 
     1381 CAGAGGACTG CTTATACCTC AACATTTATG CACCATGCCA TGCCAATAAC GGCTCCAGTC 
     1441 TCCCGGTCAT GGTGTGGATA CCAGGTGGTG GCTTTGAGAC TGGCTCAGCC TCCATCTTTG 
     1501 ATGGCTCTGC CCTGGCTGTC TATGAGGATG TGTTGGTTGT CACCATCCAG TACCGCCTAG 
     1561 GAATATTTGG CTTCTTCACC ACACAGAACC AGCATGCCCC TGGGAATTGG GCCTTCTGGG 
     1621 ACCAGCTGGC TGCCTTGCTG TGGGTCAGGG AGAACATCAA GTACTTCGGT GGAAACCCTG 
     1681 ACTCCGTGAC CATCTTCGGT AACTCAGCGG GAGCCATAAG CATTTCTAGC CTCATTCTGT 
     1741 CTCCTTTGAG TGCGGACTTA TTCCACAGAG CCATCATGCA GAGTGGAGTG GCCATCATCC 
     1801 CCAGCCTGAA GAGCTCTGAT AATGATCTGA AACATGATTT GCAGGTGGTT GCAAATGTTT 
     1861 GTGATTGCAA TGTATCAGAC TCCAAGGCTT TGCTGAAGTG CCTGAGGGAA AAGTCTTCTT 
     1921 TGGAGTTGAT GAGCCTTAGC CAGAAAGCAA AATCATTCAC TCGAGTGGTC GACGGTTCTT 
     1981 TCTTCTCCGA AGAACCACTA GAACTGTTGT CTCAAAAGAC ATTGAAAATA GTACCTTCTA 
     2041 TCATTGGAGT CAATAACCAG GAGTGTGGCT ACATATTGCC CGTGAGAGAC ACTCCTGAGA 
     2101 TTCTCCTCGG CTCCAATGAG TCTACTGCTC TCACCTTGAT ACATACTTTG CTGCATATCC 
     2161 CCACCCAGCA TTTGTACATT GTGACTAAAG AGTACTTCCA TGGCAAGCAT TCTCCGACCG 
     2221 ACATCCGAGA CACTTTACTG GACTTGTTTG GAGATGTGTT CTTTGTGGTT CCCGGGCTGG 
     2281 TTACAGCTCG ATATCACAGA GATTCTGGTG GACCTGTCTA TTTTTATGAG TTTCAGCACA 
     2341 GACCCCATTG CTTTCAAAAC TCAAGGCCAG CCTTCGTGAA GGCTGACCAC ACAGACGAAA 
     2401 TCCGCTTCGT TTTTGGAGGC CCTTTCCTGA AGGGCGACGT GGTCATGTTT GAGGAAGCCA 
     2461 CCGAGGAAGA GAAGTTGCTA AGCAGGAAGA TGATGAAATA CTGGGCTAAC TTTGCCAGGA 
     2521 GCGGGGATCC TAACGGTGCC GACCTGCCTC CGTGGCCAGT CTATGATGAG AATGAACAGT 
     2581 ACCTGGAACT GGATGTGAAC ATCAGCACTG GGCGGAGACT GAAAGACCAG AGGGTGGAGT 
     2641 TTTGGACAGA CACCCTCCCC CTGATCCTGT CTGCTTCCAA AGCGCTGCTC AGTCCCACGT 
     2701 TCTCCTTAAT CCTCCTATCA CTACTGTCGC CTGTCCTTCT CTCCGCTGCT TCTACGCGTA 
     2761 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     2821 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     2881 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     2941 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3001 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3061 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3121 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3181 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3241 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3301 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3361 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3421 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3481 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3541 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     3601 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     3661 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     3721 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     3781 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     3841 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     3901 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     3961 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4021 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4081 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4141 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4201 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4261 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4321 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4381 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4441 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4501 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4561 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     4621 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     4681 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     4741 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     4801 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     4861 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     4921 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     4981 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5041 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5101 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5161 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5221 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5281 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5341 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5401 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5461 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5521 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     5581 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     5641 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     5701 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     5761 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     5821 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     5881 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     5941 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6001 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6061 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6121 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6181 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6241 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6301 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6361 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6421 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6481 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6541 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     6601 AATTTT
//