LOCUS       MR219273                8178 bp    DNA     circular  SYN 
DEFINITION  Pde3b (Myc-DDK-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b)
ACCESSION   NM_011055
VERSION     NM_011055.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4335..4426 
                     /label="Myc-DDK"  
     misc_feature    4456..5041 
                     /label="PolyA signal"  
     misc_feature    4513..4532 
                     /label="Restriction Enzyme"  
     misc_feature    5317..5989 
                     /label="ColE1"  
     misc_feature    6419..7213 
                     /label="Kan/Neo"  
     misc_feature    7248..7550 
                     /label="SV40 ori"  
     misc_feature    7636..8113 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4317..4323 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4317 
                     /label="Pde3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAAAGAC GAGCGCGAGC GGGACGCGCC AGCCATGAGG TCCCCGCCGC 
     1081 CGCCGCCAGC CTCGGCCGCC TCGCCCCCCG AGAGCCTGCG CAACGGCTAC GTGAAGAGCT 
     1141 GCGTGAGCCC GCTGCGGCAG GACCCTCCGC GCAGCTTCTT CTTCCACCTC TGCCGCTTCT 
     1201 GCAACGTGGA GCCGCCGGCG GCCTCGCTCC GCGCCGGGGC ACGCCTCTCG CTCGGCGTCC 
     1261 TGGCCGCCTT TGTCCTGGCC GCGCTGCTGG GCGCGAGGCC CGAGCGCTGG GCGGCCGCGG 
     1321 CAGCCGGGCT CCGGACGCTG CTGAGCGCCT GCTCGCTCAG CCTCAGCCCG CTCTTCAGCA 
     1381 TCGCCTGTGC CTTCTTCTTC CTCACCTGTT TCCTGACGCG CGCGCAGCGC GGCCCGGGCC 
     1441 GCGGCGCCGG CTCCTGGTGG CTGCTGGCGC TGCCCGCCTG CTGCTACCTG GGCGACTTCG 
     1501 CGGCGTGGCA GTGGTGGTCG TGGCTGCGTG GGGAGCCGGC GGCGGCGGGC CGGCTCTGCC 
     1561 TGGTGCTGAG CTGCGTGGGG CTGCTGACGC TCGCGCCCCG CGTGAGGCTG CGGCACGGCG 
     1621 TCCTGGTGCT GCTCTTCGCC GGCCTGGTGT GGTGGGTGTC CTTCTCCGGC CTCGGGGCTC 
     1681 TGCCGCCCGC GCTCAGGCCT CTGCTGTCGT GCCTGGTCGG GGGCGCGGGA TGCCTGCTAG 
     1741 CCCTGGGCTT GGACCACTTC TTTCACGTCC GGGGAGCCTC CCCTCCGCCG CGCTCGGCGA 
     1801 GTACCGCGGA GGAAAAAGTG CCTGTGATCA GACCCCGGAG GAGGTCCAGC TGCGTGTCGC 
     1861 TGGGAGAAAG CGCAGCCGGT TACTATGGCA GTGGCAAGAT GTTCAGGAGA CCGTCGTTGC 
     1921 CTTGTATTTC CCGAGAACAG ATGATCCTCT GGGACTGGGA CTTGAAGCAG TGGTGTAAAC 
     1981 CTCATTACCA GAATTCTGGA GGTGGAAATG GAGTTGATCT TTCAGTGCTA AATGAAGCTC 
     2041 GCAATATGGT GTCAGACCTG CTGATTGACC CAAGCCTTCC CCCACAAGTC ATTTCTTCTC 
     2101 TGCGGAGTAT CAGTAGCTTG ATGGGTGCTT TCTCAGGTTC CTGTAGGCCA AAGATTAATT 
     2161 CTTTTACACC ATTTCCTGGA TTTTATCCCT GCTCTGAAGT AGAAGATCCA GTTGAGAAAG 
     2221 GAGATCGAAA ACTTCACAAG GGATTGAGTG GCAGAACCAG TTTCCCAACT CCACAGCTGA 
     2281 GGAGGAGCTC TGGAGCCTCA TCGTTGCTGA CTAATGAGCA CTGTTCAAGG TGGGATCGCA 
     2341 GCAGTGGTAA GAGGTCTTAC CAAGAACTCA GTGTTTCAAG TCATGGATGC CACCTAAATG 
     2401 GGCCTTTTAG TTCAAATCTT TTTACAATTC CAAAGCAGAG GTCATCGTCT GTGTCACTGA 
     2461 CGCACCATGC AGGTCTGAGA AGAGCTGGTG CTTTGCCCAG CCACAGTCTT CTGAATTCTT 
     2521 CAAGCCATGT ACCAGTGTCT GCTGGCTCTC TAACTAATCG ATCACCCATA GGATTTCCTG 
     2581 ATACCACTGA TTTTCTTACT AAGCCAAATA TTATTTTACA TAGATCACTG GGCAGTGTAT 
     2641 CAAGTGCAGC AGATTTCCAT CAGTACCTTA GGAACTCTGA CAGCAATCTG TGTAGCAGCT 
     2701 GTGGACACCA AATACTCAAA TATGTTTCAA CATGTGAACC CGATGGTACA GACCACCCCA 
     2761 GTGAAAAATC AGGTGAAGAA GACAGCAGTG TTTTCTCAAA AGAACCATTG AACATTGTGG 
     2821 AAACCCAAGA AGAAGAGACC ATGAAGAAAG CCTGCAGGGA GTTATTTTTG GAAGGTGATA 
     2881 GTCACCTGAT GGAAGAGGCA CAGCAACCAA ATATCGATCA GGAAGTGTCA CTGGATCCAA 
     2941 TGTTAGTAGA AGATTATGAT TCATTAATAG AAAAGATGAA CAACTGGAAT TTTCAGATTT 
     3001 TTGAGCTTGT AGAAAAGATG GGAGAAAAAT CAGGAAGGAT TCTCAGTCAG GTTATGTATA 
     3061 CTTTATTTCA AGATACTGGT TTATTGGAAA CATTTAAAAT TCCCACTCAA GAATTTATGA 
     3121 ATTATTTTCG TGCCTTAGAA AATGGCTACC GGGACATTCC ATATCACAAT CGTGTGCATG 
     3181 CCACAGATGT CCTACATGCT GTTTGGTATT TGACAACCCG ACCAATTCCT GGCTTACCTC 
     3241 AGATCCATAA TAACCATGAA ACGGAAACCA AAGCAGATTC AGATGGTAGA CTTGGTTCTG 
     3301 GACAGATTGC TTACATTTCT TCGAAGAGTT GCTGTATTCC AGATATGAGT TATGGCTGCC 
     3361 TATCTTCAAA CATCCCTGCA TTAGAATTGA TGGCTTTGTA TGTGGCAGCT GCCATGCACG 
     3421 ATTACGATCA CCCAGGAAGA ACAAATGCGT TTCTAGTGGC TACAAATGCA CCTCAGGCAG 
     3481 TTTTATACAA TGACAGATCT GTTCTAGAGA ATCATCATGC TGCATCAGCT TGGAATCTGT 
     3541 ATCTTTCTCG CCCAGAGTAC AACTTCCTCC TTAACCTTGA TCACATGGAA TTCAAGCGGT 
     3601 TTCGATTTTT AGTTATAGAA GCAATCCTTG CTACAGATCT CAAAAAACAT TTTGATTTCC 
     3661 TTGCAGAATT CAATGCCAAG GCCAATGATG TAAATAGTAA CGGTATAGAA TGGAGCAGTG 
     3721 AAAACGATCG CCTCTTGGTC TGCCAGGTGT GCATCAAATT AGCAGATATC AATGGCCCAG 
     3781 CAAAAGATCG GGACCTACAT TTGAGATGGA CAGAAGGCAT TGTGAATGAA TTTTATGAGC 
     3841 AGGGAGATGA AGAAGCAACC CTGGGTCTAC CTATTAGTCC CTTCATGGAT CGTTCTTCTC 
     3901 CTCAACTAGC AAAACTCCAA GAATCTTTTA TCACCCACAT TGTGGGCCCC CTGTGCAACT 
     3961 CCTATGATGC TGCTGGCTTG CTGCCGGGTC AGTGGATAGA AACAGAAGAG GGTGATGATA 
     4021 CAGAAAGTGA TGATGATGAT GATGATGATG ATGGTGGTGG TGAAGAATTA GATTCAGATG 
     4081 ATGAAGAAAC AGAAGACAAT CTAAATCCTA AACCACAAAG AAGGAAAGGC AGGCGGCGAA 
     4141 TATTTTGCCA ACTAATGCAC CACCTCACTG AAAACCACAA GATATGGAAG GAAATCATAG 
     4201 AAGAAGAAGA AGAAAAATGT AAAGCTGAGG GGAACAAACT GCAGGTGGAT AATGCCTCCT 
     4261 TACCTCAGGC AGATGAGATT CAGGTTATTG AAGAAGCAGA TGAAGAGGAA GAACAAATGT 
     4321 TTGAAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4381 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4441 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4501 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4561 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4621 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4681 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4741 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4801 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4861 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4921 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4981 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5041 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5101 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5161 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5221 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5281 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5341 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5401 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5461 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5521 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5581 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5641 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5701 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5761 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5821 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5881 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5941 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6001 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6061 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6121 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6181 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6241 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6301 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6361 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6421 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6481 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6541 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6601 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6661 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6721 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6781 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6841 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6901 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6961 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7021 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7081 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7141 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7201 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7261 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7321 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7381 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7441 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7501 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7621 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7681 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7741 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7801 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7861 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7921 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7981 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8041 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8101 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8161 AAATTTAACG CGAATTTT
//