LOCUS       MR219269                8610 bp    DNA     circular  SYN 
DEFINITION  Tbc1d4 (Myc-DDK-tagged) - Mouse TBC1 domain family, member 4 (Tbc1d4)
ACCESSION   NM_001081278
VERSION     NM_001081278.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4767..4858 
                     /label="Myc-DDK"  
     misc_feature    4888..5473 
                     /label="PolyA signal"  
     misc_feature    4945..4964 
                     /label="Restriction Enzyme"  
     misc_feature    5749..6421 
                     /label="ColE1"  
     misc_feature    6851..7645 
                     /label="Kan/Neo"  
     misc_feature    7680..7982 
                     /label="SV40 ori"  
     misc_feature    8068..8545 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4749..4755 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4749 
                     /label="Tbc1d4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCGCCT AGCTGCATTC AGGATGAGCC TTTCCCGCAC CCCCTGGAGC 
     1081 CTGAGCCGAG TGCCCCAGCA CAGCCAGGTG CCACCAAGCC TGGCGACAAG CGCTTCCGGC 
     1141 TATGGTACGT GGGAGGGTCT TGCTTGGATC GCAGGACCAC GCTACCCATG CTCCCTTGGC 
     1201 TCATGGCCGA GATCCGTCGG CGCAGCCAGA AGCCCGATGC GGGCGGCTGC GGGGCGCCCG 
     1261 CAGCCCGGGA GGTGATCCTG GTGCTCAGCG CCCCCTTTCT GCGTTGTGTC CCTGCGCCGG 
     1321 GTGCCGGGGT CGGCGGGGGC GCAGGCTCCG GGGCTGTGCA GCCCAACACC GGGGTGTTCA 
     1381 TCTTCGAGCA CAAGGCTCAG CACATCTCGC GCTTCATCCA CAACAGTCAC GACCTCACCT 
     1441 ACTTTGCCTA TTTGATCAAG GCGCAGCCCG ACGACCCCGA GTCACAGATG GCCTGCCACG 
     1501 TTTTCCGCGC CACGGATCCT AACCAGGTTC CAGATGTGAT CAGTAGCATC AGGCAGTTAT 
     1561 CCAAGGCTGC CATGAAGGAG GACTCCAAAC CCAGCAAAGA TAACGAGGAT GCCTTCTACA 
     1621 ACTCTCAGAA ATTCGAAGTG CTTTACTGTG GGCGGGTGAT CGTGACCCAT AAGAAGGCGC 
     1681 CCTCCAGCCT CATTGATGAC TGCAAGGACA AGTTCAGCCT GCATGAGCAG CAGCGACTGA 
     1741 AGCTGCAAGG CGAGAGAGGT GGTGACCCTG GCGACGAGAT GGGTGTCCTT GAGGTCGAGT 
     1801 CTCCTGTGTC TCCAGATGAC AGCCTGCCCG AGAAGGCAGA TGGTACAGTC AACAGCCCCC 
     1861 GTGCCCTGCC CTCTCTGGCT AGCCTTCCTG CCTTGGCCAG CCAACCCGCA CTAGCCAGCT 
     1921 CTCGGGTTTG CTTCCCAGAG AGGATTTTGG AAGACTGTGG CTTTGACGAG CAGCAAGAGT 
     1981 TCCGATCTCG GTGCAGCAGC GTCACAGGCG TCATGCAAAA GAAGGTTCAT GAGAACAACC 
     2041 AGAAAACACA GCCAAGAAGG AGGCACGCGA GCGCGCCCAG TCACGTGCAG CCCTCAGACT 
     2101 CGGAGAAGAA TCGGACCATG CTCTTCCAGG TCGGCCGCTT TGAGATTAAC CTCATCAGTC 
     2161 CCGACACTAA ATCAGTCGTG CTGGAGAAGA ATTTCAAAGA TATCTCCTCT TGTTCTCAGG 
     2221 GAATAAAGCA TGTTGATCAC TTTGGCTTTA TCTGTCGGGA GTCTCCGGAG CCGGGGCTGA 
     2281 GCCAGTACAT CTGTTATGTG TTCCAGTGCG CCAACGAATC TCTGGTGGAT GAGGTCATGC 
     2341 TGACTCTGAA GCAAGCTTTC AGCACGGCTG CTGCGCTGCA GAGTGCCAAG ACGCAGATTA 
     2401 AACTGTGCGA GACCTGCCCC ATGCACTCTT TGCATAAGCT CTGTGAGAGA ATCGAAGGTC 
     2461 TCTACCCACC TCGAGCCAAG CTGGTAATCC AGAGGCACCT CTCTTCGCTG ACGGATAACG 
     2521 AGCAAGCTGA CATCTTTGAA CGGGTTCAGA AGATGAAGCC CATCAGCGAC CAGGAGGAGA 
     2581 ACGAACTTGT GATTCTGCAC CTGAGGCAGC TCTGTGAAGC CAAGCAGAGG ACCCACGTGC 
     2641 ACATCGGGGA AGGGCCGGCG ATTATTTCAA ATAGTACAAT CCCAGAAAAT GTGACAAGCG 
     2701 GTGGGAGGTT CAAGCTTGAT GTTCTGAAAA ACAAAGCGAA GAGATCCTTA ACTTCCTCCC 
     2761 TGGAAAATAT CTTCTCCAGG GGAGCTAACA GGATGAGAGG TCGGCTTGGA AGTATGGACA 
     2821 GCTTTGAACG CGCCAACAGT CTTGCCTCAG AGAAGGACTT CTCACCAGGG GACTCTCCTC 
     2881 CTGGGACACC GCCAGCCTCC CCACTGTCCT CCGCCTGGCA CGCCTTCCCA GAGGAGGACT 
     2941 CCGACTCTCC ACAGTTCCGA AGACGGGCTC ACACATTCAG CCATCCACCT TCAAGTTCAA 
     3001 GGAGGAAGCT GAACTTGCAA GATGGCAAGG CCCACGGCCT ACGGTCCCCT CTACTGAGGC 
     3061 AGAGCTCCAG CGAACAATGC AGTGATGGAG AAGGGAGAAA GAGGACGTCA TCCACCTGCA 
     3121 GCAACGAGTC CCTAAATGCC GGAGGAACCC CGGTCACTCC TCGCAGAGTC TCCTGGAGAC 
     3181 AGCGGATTTT CCTCCGGGTT GCATCTCCTG TGAACAAGTC CCCTTCAGCA ATGCAGCAGC 
     3241 AAAAAGATGG GCTAGACAGG ACTGAGCTGC TCCCGTTGTC TCCTCTCTCT CCCACCATGG 
     3301 AGGAAGAGCC TCTCATTATC TTCTTGTCTG GTGACGAGGA CACAGAAAAA GTTGAAGAAA 
     3361 AAAAGAAATC AAAGGAGCTC AAGAGTCTGT GGAAAAAAGC TATACACCAG CAAATCCTGC 
     3421 TGCTTCGAAT GGAGAAGGAA AACCAGAAAC TGGAAGCAAG AAGAGATGAG CTCCAATCTA 
     3481 GAAAAGTCAA GTTGGACTAT GAAGAAGTTG GGACATGTCA GAAGGAGATC TTAATAGCAT 
     3541 GGGACAAGAA GCTGTTAAAT TGCAGAACTA AAATCAGGTG TGACATGGAA GATATCCATA 
     3601 CGTCCCTTAA AGAAGGGGTC CCTAAAAGTC GGCGAGGGGA AATTTGGCAA TTTCTAGCAT 
     3661 TACAATATCG CCTAAGACAC AGATTGCCCA ACAAACACCA GCCTCCAGAC ACCTCCTATA 
     3721 AGGAGCTTTT GAAGCAGCTC ACTGCTCAGC AGCACGCCAT CCTTGTGGAT TTGGGGAGGA 
     3781 CTTTTCCCAC CCACCCTTAC TTTTCAGTGC AGCTTGGGGC AGGACAGCTG TCCCTCTTTA 
     3841 ACCTGCTGAA AGCCTACTCT CTGCTGGACA AAGAGGTAGG CTACTGTCAG GGCATCAGCT 
     3901 TTGTGGCCGG AGTCCTGCTT CTGCACATGA GTGAAGAGCA AGCCTTTGAA ATGCTCAAGT 
     3961 TCCTAATGTA CGACCTGGGC TTCCGAAAGC AGTACCGACC GGATATGATG TCACTTCAGA 
     4021 TTCAGATGTA CCAACTGTCC AGACTCCTTC ATGACTACCA CCGAGAACTC TACAATCATC 
     4081 TTGAGGAAAA TGAGATCAGC CCCAGTCTGT ACGCTGCTCC CTGGTTCCTC ACACTCTTTG 
     4141 CCTCTCAGTT TCCCTTAGGA TTCGTAGCCA GAGTTTTTGA TATTATTTTC CTTCAAGGAA 
     4201 CTGAAGTTAT ATTCAAGGTT GCTCTCAGCT TACTGAGCAG CCAGGAGGCA CTTATAATGG 
     4261 AGTGTGAAAA CTTTGAAAAT ATTGTTGAAT TTCTCAAAAG TACATTACCT GATATGAATA 
     4321 CCACTGAAAT GGAAAAAATT ATTACCCAGG TTTTTGAGAT GGATATTTCC AAACAGTTAC 
     4381 ACGCCTATGA GGTAGAATAT CACGTGCTGC AGGATGAGCT GCTAGAATCT TCCTATGCCT 
     4441 GTGAGGACAA TGAATCTCTG GAGAAGCTGG AGAGAGCCAA CAACCAATTG AAAAGACAGA 
     4501 ACATGGACCT CTTGGAAAAA CTCCAGGTGG CTCATGCTAA AATTCAGGCC TTGGAATCCA 
     4561 ACCTAGAAAC TCTTTTGACC AGAGAAACCA AAATGAAGGC TTTAATCCGG ACCCTGGAAC 
     4621 AAGATAAAAT GGCATATCAA AAGACAGTGG AACAGATCCG GAAGCTGCTA CCAGCAGATG 
     4681 CGCTGGCCAA CTGTGAATTG CTGTTGAAAG ACTTAACTCA TCCAACGAAT GACAAAGCCA 
     4741 AGGCAGGAAA TAAGCCAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4801 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4861 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4921 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4981 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5041 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5101 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5161 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5221 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5281 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5341 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5401 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5461 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5521 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5581 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5641 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5701 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5761 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5821 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5881 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5941 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6001 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6061 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6121 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6181 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6241 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6301 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6361 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6421 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6481 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6541 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6601 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6661 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6721 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6781 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6841 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6901 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6961 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7021 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7081 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7141 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7201 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7261 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7321 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7381 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7441 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7501 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7561 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7621 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7681 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7741 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7801 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7861 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7981 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8041 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8101 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8161 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8221 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8281 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8341 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8401 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8461 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8521 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8581 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//