LOCUS       MR219224                8625 bp    DNA     circular  SYN 
DEFINITION  Vwa5b2 (Myc-DDK-tagged) - Mouse von Willebrand factor A domain containing 5B2 (Vwa5b2), transcript variant 1
ACCESSION   NM_001144953
VERSION     NM_001144953.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4782..4873 
                     /label="Myc-DDK"  
     misc_feature    4903..5488 
                     /label="PolyA signal"  
     misc_feature    4960..4979 
                     /label="Restriction Enzyme"  
     misc_feature    5764..6436 
                     /label="ColE1"  
     misc_feature    6866..7660 
                     /label="Kan/Neo"  
     misc_feature    7695..7997 
                     /label="SV40 ori"  
     misc_feature    8083..8560 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4764..4770 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4764 
                     /label="Vwa5b2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGGCCTG TACTGCCCCA CCAGCTGGAC GCCGCTGCCG CTCACCGACT 
     1081 CCTGCGTCCG GGCCTATGCC AAGGGACCCT GCCTCAGCCT GCGTGCCCGG CTTACCTACC 
     1141 ACAACCCACA GCCCCAGCCG GTGGAAGGCG TGTTCGTGTA CCCTCTGGCT GAGGCGGAGG 
     1201 TGGTATCTGG CTTCGAGGCC GAGGCGGCCG GACGGCGCGT CTCCTTCCAG TTGCATAGCC 
     1261 GGCGCCGATC ACAGGCCGCC TGCTGTCGCG CTCTGGGCCC GGGACTAGGG ACCTCTACAC 
     1321 CCCGCCGCTG CGCACAGGGT CATCTTGTCT TGAATCTGGC CCAAGCCCGG TCCACACTGG 
     1381 TGTTGCCCAC TGGCCTTGTG GCTGCAGCTG GTACCATGAC AGTGACCCTG TGCAGCAGCC 
     1441 GGGAGTTGCC CTCCAGGCCT GATGGGGTGA TGCATGTGGC CCTGCCCACT GTGTTCACCC 
     1501 CACTGGCCCA GCCAAACCTG CCAGGGTCCC CCAGGTCTCC GGGGCTCTGT GACGACAGCC 
     1561 CCACCAGCTG CTTCGGGGTA GGCAGCCCTG AGGAGGAAAG GCCGACCTGG GAGCAACCAA 
     1621 CTGCCACTCC GGACGTGTTC TCAGGCCCTG CCCGCTGTCC TGCTCCGTAT ACCTTCTCTT 
     1681 TCGAGATGCT AGTGACCGGA CCATGTCTGT TGGCAGGCCT GGAGAGCCCC TCTCATGCTC 
     1741 TGCGTGCAGA TGCCCTCCCT CATGCCAGCT CTGCAGCAAC TATCCGTGTC ACCCTGGCAG 
     1801 AGGGCCACCA GTGTGATCGG GCCTTGGAGA TCCTCTTGCA CCCCAGTGAG CCCCATCAGC 
     1861 CACACTTGAT GCTGGAGACT GGCAGCTTGA GTTCAGCAGA ATATGAGGCC CAAGTGAGGG 
     1921 CCCGCCATGA CTTCCAGAGG TTGCAGCAAA GAGACAGTGG TGGGGAACGG CAGGTCTGGT 
     1981 TCCTGCAGCG ACGGTTCCAT AAGGACATCT TGCTGAACCC TGTGCTGGTG CTGAACTTCT 
     2041 GCCCAGACCT GAGCTCCAAG CCTGGACACC TGAATGCAGC TACCCGGGAG CTCCTCTTCC 
     2101 TGTTAGATGG CAGTGGTGCA GGACACAAGG ATGCCATTGT TTTGGCTGTA AAATCCCTCC 
     2161 CAGCTCAGAC TCTAGTCAAC CTAGCCATAT TTGGGACATT GGTACAGCCA CTCTTCCCAG 
     2221 AGAGCCGGCC TTGCAGTGAT GACACTGTGC AGCTGATCTG CGAGAGCATC GAGACTCTCC 
     2281 AGACTGTGAA TGGTCCCCCT GATATGCTGG CTGTATTGGA TTGGGCCTTA GGGCAGCCCC 
     2341 AGCACAGGGC CTACCCTCGA CAGATGTTCC TGATCACTGC TGCCTCGCCA ACGGCTGCCA 
     2401 CCACTCACCA AGCCCTGGAG TTTATGAGGT GGCACAGAGG GGCAGCCAGG TGCTTCTCCT 
     2461 TTGCATTGGC ACCTGCCTGC CGCCAGCTGC TCCATGACTT GTCTGTCCTC AGCAGAGGAC 
     2521 AGGCTTACTT CCTGAGGCCC GGGGAGAGAC TTCAGCCCAA GCTGGTACAG GCTCTGCGGA 
     2581 AGGCACTGGA ACCTGCTTTG AGTGACATCT CTGTGGACTG GTTTGTGCCT GATGCTGTGG 
     2641 AGGCACTGCT GACACCCCGG GAGATCCCAG CTCTCTACCC TGGGGACCAG CTGCTTGGCT 
     2701 ACTGCTCGCT TTTCAGGGTG GATGGCTTCC GGTCCCATGC TCTAGGGGGA CAAGAGCCTG 
     2761 GTTGGCAGAG CTTGGCCGGT TCAGTCTTCC CATCTCCAGA GGAGGTGTTA TCCGCCACCA 
     2821 GCCCTGGCAC TGAGCCTACG CACACCACAG AGCCCCTGGG AACAGGCACT GTGTCAGCAG 
     2881 AGCTGTCGAG CCCATGGGCT GTAGGCGACT CAGAGCAGAG TATGGAAGCT CTGACAGACC 
     2941 CAGTCATGGA TCCTGGACCT AATCCCTCAT CTGACACAGC TATATGGCGG CGCATCTTCC 
     3001 AGTCCTCATA CATCCGGGAG CAGTATGTGC TTACCCACTG CTCTGCCAGC CCTGAGCCAG 
     3061 GCCCAGGCTC CACCTGCAGT AGCGAGTCCC CAGGTTCCCA AGGCCCTGGC TCCCCCAGTG 
     3121 GTAGCCGTCC CCTGGATCCC CCTTCTCAGC AGGGCTGCCG AAGCCTGGCC TGGGTAGAAC 
     3181 CTGCAGGCTC CCGCTCCTGC CCCCTGCCTG TACCCCCACC ATCTCCATTC AAGGTGGGAG 
     3241 CCATGAGCGC TGAGGTGCTG GGCCGTCGGC AAAGAGCAGC TCTGGCTGGT CGAAGTCTCT 
     3301 CATCTCCCTC AGGCCGGGCA AACCCAGTCC CCGGAAGAGC CCGGCACCCC TCTCTAGATG 
     3361 CAATACCAGA TGGATTAGGC CCTGAGCCAG GACAACAGCT GGGTCAGGGC CTCGATGATT 
     3421 CAGGAAACCT GCTCTCCCCA GCCCCCTTGG ACTGGGATAT GTTGATGGAA CCATCTTTCT 
     3481 TATTCAAGCC TGTGCCGTCC AGTGCGGAAT CAGCTCCTCC AGCAGAATGT CTGCCTCCTC 
     3541 AGGCTCCACG TTGCCACGTG GTGATCCGGG CCCTGTGTGG GGAACAGCCA ATGTGCTGGG 
     3601 AGGTGGGTGT TGGGCTGGAA GAACTCTGGG GTCCTGGGGA TGGCTCCCAG CCTGAATCAC 
     3661 TTCCGATGAG AGAAGCTGCG TGGGATCAGG CACTCCACCG GCTGACAGCA GCCTCTGTGG 
     3721 TCCAGGACAA TGAACAGCTG GCTCTCCGTG GAAGAGCTGA AACCAGGGCT GAGCAGGGCA 
     3781 GAGTCAGAAG GTCCTGGCTC AGAGCCATTC AGACAAGCAA GGTCAGCTCT GCCCCATCCT 
     3841 GTTTTACCTG CCCTGTTGCT GTAGATGCTA CCACTAGGGA GGTATTGCCT GGAGCCCTGC 
     3901 AGGTGTGGAG TTCAGATCCA GCTGAGCTCT CAGGCATGTC TGCCTCTCAA GACCAGCTAG 
     3961 CTGCCGCTCC TCTGTCCACA GCAGTCCACT CTAAAGGACA TCAGGGAGGC TGTTCTGCAG 
     4021 GTGCCTGGGA CCTGGACCTA AATGACAACT CCAAGTCAGC ATTGGGGGAG CCCATATCTC 
     4081 CTACTGGAGA TCATCATGGC TTGCCCCACC AGCCTCCAGC CTCTTCCAGG CTCAGCCTGG 
     4141 GTCGTCATCG AAGACTCTGC AGCTCCAATA AGGGCCAGAC TCATGAGAAC AGCAATGACG 
     4201 GCAGCAACCA TGACTACCTG CCTCTGGTAC GACTGCAAGA GGCACCAGGC TCCTTCCGCC 
     4261 TGGATGAACC CTTCTGTGCT GCTGTGTGCA TTCCTCAAGA ACGCCTGTGT CGAGCCTCGC 
     4321 CCTTTGCCGC ACACCGTGCC AGCCTCAGCC CCACCTCAGC ATCATCTCCT TGGGCATTTC 
     4381 TGAGCCCTGG TATTGGCCAA GGTGACAGTG CCACAGCCTC CTGCAGCCAG TCCCCCAGCT 
     4441 CAGGTTCTGA GGGTCCAGGC CAGGTAGACA GTGGGAGGGG CTCAGATACT GAGGCCTCAG 
     4501 AAGGAATGGA AAGGCAGGAC AGCTCTGATC TTCGTGGGCG CACTTGGGCC ACAGCTGTGG 
     4561 CCCTGGCGTG GCTGGAACAC CGCTGTGCAG CTGCCTTTGG CGAGTGGGAA CTGACAGCTT 
     4621 CCAAAGCTGA TTGCTGGCTG AGAGCCCAAC ACCTGCCAGA CGGACTTGAT CTGACTGCTC 
     4681 TTAAAGCTGC TGCCCGGGGG CTCTTTCTGT TACTGCGACA CTGGGACCAG AATCTGCAGC 
     4741 TACACTTGCT GTGTTACAGC CCTTCGAATG TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4801 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4861 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4921 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4981 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5041 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5101 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5161 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5221 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5281 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5341 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5401 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5461 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5521 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5581 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5641 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5761 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5821 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5881 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5941 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6061 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6121 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6181 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6241 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6361 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6421 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6481 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6541 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6601 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6661 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6721 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6781 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6841 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6901 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6961 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7021 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7081 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7141 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7201 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7261 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7321 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7381 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7441 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7501 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7561 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7621 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7681 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7741 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7801 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7861 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8041 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8161 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8221 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8281 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8341 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8401 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8461 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8521 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8581 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//