LOCUS       MR219220                8037 bp    DNA     circular  SYN 
DEFINITION  Clmn (Myc-DDK-tagged) - Mouse calmin (Clmn), transcript variant 1
ACCESSION   NM_053155
VERSION     NM_053155.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4194..4285 
                     /label="Myc-DDK"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    4372..4391 
                     /label="Restriction Enzyme"  
     misc_feature    5176..5848 
                     /label="ColE1"  
     misc_feature    6278..7072 
                     /label="Kan/Neo"  
     misc_feature    7107..7409 
                     /label="SV40 ori"  
     misc_feature    7495..7972 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4176..4182 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4176 
                     /label="Clmn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCTCAG GAGTGGGACT GGTTCCAGCG CGAGGAGCTC ATCGGGCAGA 
     1081 TCAGCGACAT CCGTGTGCAA AACCTGCAAG TGGAGAGGGA AAACGTGCAG AAGAGGACTT 
     1141 TCACACGATG GATAAACCTA CACCTCGAAA AGTGTGACCC ACCTCTAGAA GTTACGGACC 
     1201 TGTTTGTTGA TATTCAAGAT GGCAAAATCC TAATGGCTCT GTTAGAAGTC CTCTCTGGGC 
     1261 GAAATCTGCT GCATGAATAC AAATCCTCAT CACATCGTAT TTTTCGGTTG AACAACATAG 
     1321 CGAAAGCACT CAAGTTTCTG GAAGATAGCA ATGTCAAGCT GGTTAGCATC GATGCAGCGG 
     1381 AAATAGCGGA CGGCAACCCC TCGCTGGTTC TCGGGCTGAT ATGGAACATC ATTCTCTTCT 
     1441 TCCAGATAAA GGAGCTCACG GGCAACCTCA GCAGGAGTTC TCCATCTTCC AGCTTGTCAC 
     1501 CGGGCTCTGG GGGCACCGAC TCCGACTCTT CCTACCCACC CACCCCCACC ACCGAGAGGA 
     1561 GTGTGGCAGT GGCAGTGAAA GACCAGAGGA AGGCCATCAA GACCCTGCTG TCATGGGTGC 
     1621 AGAGGAAAAC CAGGAAGTAT GGTGTGGCAG TCCAAGACTT TGCAGGCAGC TGGAGGAGTG 
     1681 GCCTGGCTTT CCTGGCTGTC ATCAAAGCTA TTGACCCCAG CCTTGTAGAC ATGAAGCAGG 
     1741 CTCTGGAAGA TTCCACCCGG GACAATCTGG AAAAGGCCTT CAGCATTGCC CATGATTCCC 
     1801 TGCATATCCC GAGGCTCCTG GAGCCGGAAG ACATCATGGT TGACATGCCA GATGAGCAGT 
     1861 CCATTGTGAC ATACGTGGCC CAGTTTCTAG AGCGCTTTCC AGAGTTGGAG CCTGAAGATT 
     1921 TTGTGAATCC GGACAAAGAA GCCCCCATCG AGTCCACCTT TGTCCGCATC AAAGAAAGCC 
     1981 CCTCTGAGCA GGGGAGCAGA GTCCTCCTCC TCAGCGAGAA CGGAGAGCGG GCCTACACTG 
     2041 TCAACCAGGA AACCAGCTAC CCGCCGCCTG ACAAGGTCTT CGTCTGTGAC CAGCTTGAGA 
     2101 GTCCGACTGG CTTCTGTCTG GATAGTGCTC CCAGCCACAA ACTGTCAGAC AGCTCCACAG 
     2161 AGTTTATGCA TGAGATTATT GACCAAGTCC TCCAGGGGAG CACGGGGAAG ACTGGCTCCA 
     2221 TCGCAGAGCC AACTCCAGAG TCGTCCATTT TGTCAACCAG AAAGGACGGC AGGAGGTCCA 
     2281 ACTCTTTGCC TGTCAAGAAA ACCGTCCACT TCGAGGCAGA CTTGCACAAG GATGCCTCGT 
     2341 GTAGTAAGGA CCCTTTCTAT AGCTCTGACT TCCGCTTCGA GGGGAGCCCC AAGGCAACCA 
     2401 AGGAGCTGTC AAAGCAGGAC GGCCATGTCT CACTGGCTGA GGTGTCCAAG GAAAAGAAGA 
     2461 AGTCAGAACA GGAAGCCAGG CTAGTGCTGG AAGCTGCCTC AGACAAGGTC CCCGAAAGCA 
     2521 CGGTCGATGG TCTGGATGCT GTGCCCCAAG ATGCTCAGCC TTCCCAGGAC TCCTCCTTCT 
     2581 GTAACGGCAC TGTAGAGAGC CCATCCAGCC AAGGTGAGAA AGGCCCTCCT CCATCATCCC 
     2641 CTGGAGATCA TACTCTCCTG GCCAACTCCA CTGAGCTCAA GGTTCAGCTG CTGACCGTTG 
     2701 AGCCTATGGA CAAGGAAGAC TACTTTGAAT GCATCCCACT AAAAGCTTCA AAGTTTAACA 
     2761 GGGACCTAGT GGATTTCGCC TCCACCAGCC AGGCTTTTGG TGAGGATCCT TCGTCCCACG 
     2821 AGAAGACCCG TGGGGAGGAA GAGGGTTCTG AGAATCACGC TGAGAAACCA GGGAAAAGGA 
     2881 AATCAAAGTC TCCCCGTGCA GAAACAGAGG CCGCCGAGTC CAGATTGGAG CCCAAGAAGC 
     2941 TCGAGCCTCC TCCCAAGGAT CCCGAACAAG AAGACCAAGG CCACGCTTTG CCCCCGGAAA 
     3001 CCCCTGCAGA CAAAAAGCCC AAAGTGTATG AAAAGGCCAA GAGAAAGTCC ACTCGTCATC 
     3061 ACAGCGAGGA AGAAGGAGAA GCAGAAAGTG GCTTCTCTGC AGTCTGTGAA GAAGAAATAC 
     3121 CATCCGCCCC TCCCAGCACC AGCGTCAGCC TGGAAACCCT CAGGAGTCAC AGCGAAGAAG 
     3181 GTTTGGATTT CAAACCTTCG CCGCCGCTCT CGAAGATCTC TGTCATCCCC CACGACCTGT 
     3241 TCTACTACCC TCACTACGAA GTGCCCCTGG CCGCTGTGCT GGAGGCTTAC GCGGAAGGCG 
     3301 GGGAGGATTT GAAAAGTGAA GACACAGACC TAGAGCATCC AGAGGACAGC TATCTGCAGG 
     3361 ACTCCAGGGA GGAGGAGGCC GACGAGGACG AGGAGGAGGC CCAGAGCTCT CAAAGCAGCT 
     3421 GCAGTTTCTC CTTGCCTGTT GACAACAGCT ACCCCAGCGT CAGTGAGCAT GTGTCCCATG 
     3481 TCGATGGGAG TTCTGAGGGA CCCACGTCAG CCTTGGGACC TGGCTCTCCA CCCTCTCATG 
     3541 AGGACCACCA GCCAAAGGAA ACCAAAGAGA ATGGCCCTGT GGAAAGCCAG CAGTCCCAAG 
     3601 AACCCCCAAA CCCGGAACTG CCCACAAAGC CATTAGAAGA AAAGTTAACG GAAGCCTCAA 
     3661 CTAGCAGTAA AAAGAAGGAA AAACGGAAAC ACATGGACCA CGTAGAAAGC TCATTATTTA 
     3721 TAGCCCCTGG GACGGTCCGC TCCTCAGACG ACCTTGAAGA AAACTCCAGC GAACACAAGG 
     3781 TCCCTTCCAG GAATAGTCAC AGTGACTCCA GCATTTACAT TCGGCGACAC ACTAATAGGT 
     3841 CTTTGGAATT GGACCATTTT AGCTATGTTC AATTGAGGAA CGCAGCTGAT CTGGATGACA 
     3901 GGAGAAACAG AGTGTTAAAC AGGTACAATT CTCAGAAGCT CACTGAGCTG ATTTTACAGT 
     3961 TCTACGGCAT CAGAGCAGAC ATGAAGCGCG AGTACAAACA TGCCAGGCTG TCTATGACTG 
     4021 GCACCAACTC CTCTGGAGAA GCTGTGCCCC TGGGGAACCA GAGCCCGCCC AACGACTCTC 
     4081 TGACGCAGTT TGTACAGCAG CCCGACGTGA TATATTTCAT TCTCTTCCTC TGGCTCCTCG 
     4141 TTTACTGCTT ACTGCTCTTC CCACAGCTTG ATGTCAGCCG GCTCACGCGT ACGCGGCCGC 
     4201 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4261 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4321 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4381 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4441 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4501 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4561 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4621 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4681 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4741 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4801 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4861 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4921 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4981 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5521 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5761 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5821 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5881 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5941 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6001 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6061 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6121 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6181 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6241 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6301 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6361 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6421 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6481 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6541 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6601 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6661 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6721 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6781 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6841 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6901 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6961 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7021 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7081 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7141 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7201 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7261 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7441 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7621 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7681 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7741 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7801 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7861 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7921 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7981 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//