LOCUS       MR219161                8403 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged) - Mouse synaptojanin 2 (Synj2), transcript variant 4
ACCESSION   NM_001113351
VERSION     NM_001113351.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4558..4649 
                     /label="Myc-DDK"  
     misc_feature    4679..5264 
                     /label="PolyA signal"  
     misc_feature    4736..4755 
                     /label="Restriction Enzyme"  
     misc_feature    5540..6212 
                     /label="ColE1"  
     misc_feature    6642..7436 
                     /label="Kan/Neo"  
     misc_feature    7471..7773 
                     /label="SV40 ori"  
     misc_feature    7859..8336 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4540 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGTGGGC AGAATTCCAG ATGCAGAGAT CTACAAAATC ACTGCCACTG 
     1081 AGTTGTACCC CCTGCAGGAA GAGGCCAAGG AAGAGGACCG CCTGCCCACC TTAAAGAAAA 
     1141 TCCTGAGCTC AGGGGTGTTC TATTTCGCAT GGCCCAATGA TGGCGCCTGC TTCGATCTGA 
     1201 CCATCAGGGC TCAGAAACAG GGTGATGACG GCTCTGAATG GGGGACCTCT TTCTTCTGGA 
     1261 ACCAGCTATT GCATGTGCCT CTGCGGCAGC ACCAGGTGAA CTGTCATAAC TGGTTGCTGA 
     1321 AAGTCATCTG TGGGGTGGTG ACCATCCGCA CAGTATATGC CTCCCACAAG CAGGCCAAGG 
     1381 CCTGTCTCAT CTCTCGCATC AGCTGTGAAC GCGCAGGTGC TCGCTTCCTC ACCCGTGGTG 
     1441 TGAACGATGA TGGCCACGTG TCCAACTTTG TGGAGACAGA GCAGACGATT TACATGGATG 
     1501 ATGGAGTATC GTCCTTTGTC CAGATCCGAG GCTCCGTTCC GCTGTTCTGG GAGCAACCAG 
     1561 GACTTCAGGT TGGCTCCCAT CATCTGAGAC TGCACAGAGG CCTAGAGGCC AACGCTCCTG 
     1621 CTTTTGAAAG GCACATGGTG CTTCTGAAGG AGCAATACGG TAAGCAGGTG GTTGTGAACC 
     1681 TGCTGGGTAG CAGAGGCGGT GAAGAGGTGC TCAACAGAGC CTTCAAGAAG TTGCTCTGGG 
     1741 CTTCTTGCCA CGCGGGTGAC ACACCTATGA TAAATTTTGA CTTCCATCAG TTTGCCAAAG 
     1801 GTAGGAAGCT AGAGAAATTG GAGAACCTGT TGAGACCTCA GTTACAGCTA CACTGGGAAG 
     1861 ACTTCGGCGT GTTTGCGAAG GGCGAGAATG TAAGTCCACG GTTTCAGAAA GGCACTCTGC 
     1921 GGATGAACTG TCTCGACTGT CTGGATAGAA CCAACACTGT GCAGTGCTTC ATTGCTCTTG 
     1981 AGGTCCTTCA TCTGCAGCTT GAGAGCTTGG GGCTAAATTC AAAGCCCATC ATTGACCGTT 
     2041 TTGTGGAGTC CTTCAAAGCC ATGTGGTCTC TGAATGGGCA CAGCCTGAGC AAGGTGTTCA 
     2101 CAGGGAGCAG GGCCCTGGAA GGAAAGGCCA AGGTGGGAAA GCTGAAGGAT GGGGCCCGAT 
     2161 CCATGTCTCG CACCATCCAG TCCAACTTCT TCGACGGGGT GAAGCAGGAG GCCATCAAGC 
     2221 TACTGCTAGT CGGAGATGTC TACAATGAAG AGTCTACAGA CAAAGGACGG ATGCTGCTGG 
     2281 ACAACACGGC CCTTCTGGCG ACCCCCAGGA TCTTGAAGGC CATGACAGAA CGCCAGTCGG 
     2341 AATTCACGAA TTTCAAGCGC ATCCAGATTG CTGTGGGGAC CTGGAATGTG AACGGAGGAA 
     2401 AGCAATTCCG TAGCAATCTC CTGGGGACGG CTGAGCTCAC GGACTGGCTC CTAGATGCTC 
     2461 CTCAGCTGTC AGGAGCAGTG GACTCCCAGG ATGATGGCAG TCCTGCTGAC GTATTTGCCA 
     2521 TCGGGTTTGA GGAGATGGTG GAACTGAGTG CGGGAAATAT TGTCAATGCC AGCACCACCA 
     2581 ACAGGAAGAT GTGGGGCGAG CAGCTTCAGA AAGCCATCTC CCGCTCCCAT CGGTACATCC 
     2641 TCTTGACCTC CGCACAGCTG GTGGGCGTCT GTCTTTACAT CTTTGTACGT CCGTACCACG 
     2701 TCCCGTTCAT CAGAGACGTG GCCATCGACA CCGTGAAGAC CGGCATGGGG GGAAAGGCGG 
     2761 GGAATAAGGG TGCTGTGGGC ATCCGCTTCC AGCTCCACAG CACCAGTTTC TGCTTCGTCT 
     2821 GTAGCCACCT GACGGCTGGG CAGTCTCAGG TGAAGGAGAG GAATGAAGAC TACCGGGAGA 
     2881 TCACGCACAA ACTCTCCTTC CCTTCGGGGA GAAACATATT TTCACATGAT TACGTGTTTT 
     2941 GGTGTGGCGA TTTCAACTAC CGTATTGATC TTACTTACGA AGAAGTCTTC TATTTTGTTA 
     3001 AACGCCAAGA CTGGAAGAAA CTTATGGAAT TCGATCAGTT ACAGTTACAG AAATCAAGTG 
     3061 GAAAAATTTT TAAAGACTTT CATGAAGGAG CCGTTAACTT CGGACCCACC TACAAGTATG 
     3121 ACGTTGGATC AGCTGCCTAC GACACAAGTG ACAAGTGCCG TACCCCAGCC TGGACAGACA 
     3181 GGGTGCTGTG GTGGAGGAAG AAGCATCCAT ATGATAAAAC AGCTGGTGAA CTCAACCTTC 
     3241 TAGACAGCGA TCTAGACGGC GATCCCCAAA TCAGACACAC CTGGTCTCCA GGCACTCTGA 
     3301 AATACTACGG CCGTGCAGAG CTGCAGGCGT CTGATCACAG ACCTGTGCTG GCCATTGTGG 
     3361 AGGTGGAGGT TCAAGAGGTG GATGTAGGAG CCCGGGAGAG GGTCTTCCAG GAAGTGTCCT 
     3421 CTGTCCAAGG CCCGCTGGAT GCCACCGTTG TTGTAAACCT CCAGTCTCCA ACCCTAGAAG 
     3481 AGAAAAATGA ATTTCCAGAG GACCTGCGCA CAGAACTTAT GCAGACTTTG GGGAATTATG 
     3541 GGACGATTAT TCTAGTCAGG ATCAACCAAG GGCAGATGCT GGTGACGTTT GCAGACAGCC 
     3601 ACTCGGCTCT CAGTGTGCTG GATGTGGATG GTATGAAGGT GAAAGGCAGG GCCGTGAAGA 
     3661 TTCGACCAAA GACCAAAGAT TGGCTAGAAG GCCTGAGAGA GGAGCTTCTC CGGAAGCGAG 
     3721 ACAGCATGGC CCCTGTGTCT CCCACCGCCA ACTCCTGCTT GTTGGAGGAG AACTTTGACT 
     3781 TCTCGAGTCT GGACTATGAG TCCGAAGGGG ATGTTCTTGA AGAGGATGAA GACTATTTAG 
     3841 TGGATGGGTT TGGCCAGCCT GTAGTCTCAG ACAGTGAGCT CGGTGGAGAC AACTCTTCCG 
     3901 ACACCATGAG CTCCTTGACA CCCGCCAGCA AGTCTCCCGC CCTGGCTAAA AAGAAGCAAC 
     3961 ATCCAACATA CAAAGCTGGC TTAATGGTGA AGAAGTCAGC CTCAGACGCG TCCATCTCTT 
     4021 CTGGCACTCA TGGACAATAT TCCATCTTGC AGACAGCGAA ACTTCTCCCA GGAGCACCCC 
     4081 AGCAACCACC CAAGGCTAGA ACTGGAATAA GTAAACCTTA CAACGTCAAA CAGATCAAAA 
     4141 CCACCAACGC TCAGGAGGCA GAAGCAGCTA TCCGGTGTCT CCTGGAAGCT AGCGGAGGGG 
     4201 TCCCGGAATC AGCCCCAGGT GCCATACCCC TGAGAAACCA AGGGTCTTCT AAGCCAGAGG 
     4261 CCACCCTGGG GCCCCCAGCC CTGCCCCGCC GGCCTGCTCC AAGGGTTCCC ACTATGAAGA 
     4321 AACCAACTTT GAGGAGGACA GGAAAGGTGT ACTCGGGCAT CTCCCAGTGC TTGCGTGAAG 
     4381 AGCTGCGCTC TGCTGCCTGC ACCCCACACG CAGTGAGTGC ACAGGACTGC GGAGACCTTA 
     4441 ACAACAGATG GAGAATGCCA CGTTTCTCGC ACTATATTCA CACAAAAAAA TGGAAGAACG 
     4501 TATCTCTCAG TTTTCAAGAT CTCTGGCTAA AGTTTCGCAG AAGCGGACCG ACGCGTACGC 
     4561 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4621 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4681 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4741 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4801 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4861 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4921 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4981 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5041 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5101 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5161 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5221 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5281 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5341 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5461 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5521 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5581 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5641 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5761 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5821 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5881 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5941 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6061 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6121 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6181 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6241 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6301 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6361 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6421 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6481 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6541 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6601 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6661 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6721 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6781 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6841 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6901 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6961 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7021 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7081 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7141 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7201 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7261 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7321 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7381 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7441 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7501 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7561 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7621 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7801 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7861 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7921 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7981 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8041 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8101 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8161 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8221 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8281 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8341 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8401 TTT
//