LOCUS       MR219052                7665 bp    DNA     circular  SYN 
DEFINITION  Pde2a (Myc-DDK-tagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 3
ACCESSION   NM_001143849
VERSION     NM_001143849.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3822..3913 
                     /label="Myc-DDK"  
     misc_feature    3943..4528 
                     /label="PolyA signal"  
     misc_feature    4000..4019 
                     /label="Restriction Enzyme"  
     misc_feature    4804..5476 
                     /label="ColE1"  
     misc_feature    5906..6700 
                     /label="Kan/Neo"  
     misc_feature    6735..7037 
                     /label="SV40 ori"  
     misc_feature    7123..7600 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3804..3810 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3804 
                     /label="Pde2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCTGGTG TTGCACCACA TCCTCATCGC TGTTGTCCAA TTCCTCAGGC 
     1081 GGGGCCAGCA GGTCTTCCTC AAGCCGGACG AGCCGCCGCC GCAGCCATGC GCCGACAGCC 
     1141 TGCAGGATGC TTTGCTGAGC CTAGGCGCCG TTATCGACAT TGCTGGCCTG CGACAGGCTG 
     1201 CCAGGGATGC CCTCTCAGCC GTGCTCCCCA AAGTGGAGAC TGTCTACACC TACCTGCTAG 
     1261 ATGGGGAGTC CAGACTGGTG TGTGAGGACC CCCCTCATGA GCTGCCACAG GAAGGAAAAA 
     1321 TTCGAGAAGC TGTGATCTCT CAGAAGCGGC TGAGTTGCAA TGGGCTGGGA CCTTCGGACC 
     1381 TACTGGGAAA GCCCTTGGCC AGGCTGGTGG CTCCACTGGC TCCTGACATG CAAGTGCTGG 
     1441 TCATACCCCT GCTGGACAAG GAGACTGGAA GTGTGGCAGC TGTCATCTTG GTGCACTGTG 
     1501 GCCAGCTGAG TGACAGTGAG GAACAGAGCT TGCAGGTGGT AGAGAAGCAC GCTCTGGTAG 
     1561 CCCTGCGGAG GGTGCAGGCC CTGCAGCAGC GCAGGCCTGA AGCTGTTCAG AACACGTCAG 
     1621 TGGATGCCTC TGAAGATCAA AAGGATGAGA AGGGGTACAC CGACCATGAC CGAAAGATCC 
     1681 TGCAACTGTG TGGGGAACTC TTTGACTTGG ATGCTACTTC TCTGCAGCTC AAAGTCCTTC 
     1741 AATATCTGCA GCAGGAGACA CAGGCCACTC ACTGCTGCCT CCTGCTGGTG TCGGAGGACA 
     1801 ATCTACAGCT TTCCTGCAAG GTCATTGGAG ACAAAGTGCT GGGAGAAGAG GTCAGCTTTC 
     1861 CATTGACCAT GGGACGTCTG GGGCAGGTGG TGGAAGACAA GCAGTGTATC CAGTTGAAGG 
     1921 ACCTAACCTC TGACGATGTG CAACAGCTGC AAAACATGTT GGGTTGTGAG CTGCAGGCTA 
     1981 TGCTGTGTGT CCCTGTCATC AGCCGAGCCA CTGACCAGGT GGTGGCCCTG GCTTGCGCCT 
     2041 TCAACAAGCT TGGAGGAGAT TTCTTCACAG ATGAGGATGA ACACGTGATC CAACACTGCT 
     2101 TCCACTACAC AGGCACGGTG CTCACCAGCA CCTTGGCCTT CCAGAAGGAG CAGAAGCTCA 
     2161 AGTGTGAGTG CCAGGCTCTT CTCCAAGTGG CAAAGAACCT CTTCACCCAC CTGGATGATG 
     2221 TCTCTGTCCT GCTACAGGAG ATCATCACGG AAGCCAGAAA CCTCAGCAAC GCAGAGATCT 
     2281 GCTCGGTGTT CCTGCTGGAT CAGAACGAGC TGGTGGCCAA GGTGTTCGAT GGTGGCGTTG 
     2341 TGGACGATGA GAGTTATGAG ATCCGCATCC CGGCGGACCA AGGCATCGCG GGCCACGTGG 
     2401 CGACTACGGG CCAGATCCTG AACATCCCAG ATGCATATGC CCATCCGCTT TTCTATCGCG 
     2461 GCGTAGATGA CAGCACTGGC TTCCGCACGC GCAACATTCT CTGCTTCCCT ATCAAGAACG 
     2521 AGAACCAGGA GGTCATTGGT GTGGCTGAGC TAGTGAACAA GATCAATGGG CCATGGTTCA 
     2581 GCAAGTTTGA TGAGGACCTG GCCACAGCCT TCTCCATCTA CTGTGGCATC AGCATCGCCC 
     2641 ACTCTCTCCT ATACAAAAAG GTGAATGAAG CCCAATACCG CAGCCACCTG GCCAATGAGA 
     2701 TGATGATGTA TCATATGAAG GTCTCTGATG ATGAATACAC CAAGCTTCTC CACGATGGCA 
     2761 TCCAACCTGT GGCCGCCATT GACTCCAACT TTGCCAACTT TACCTACACG CCTCGGTCTC 
     2821 TGCCTGAGGA CGACACTTCT ATGGCCATCC TGAGCATGCT GCAAGACATG AACTTCATCA 
     2881 ATAACTACAA AATTGACTGC CCAACTCTGG CCCGATTCTG CCTGATGGTG AAGAAAGGCT 
     2941 ACCGGGATCC ACCCTACCAC AACTGGATGC ACGCCTTCTC TGTCTCTCAT TTTTGCTACC 
     3001 TGCTCTACAA GAATCTGGAG CTCTCCAACT ACCTCGAGGA CATCGAGATC TTTGCATTGT 
     3061 TTATTTCCTG CATGTGTCAT GACCTGGACC ACAGAGGCAC AAACAACTCC TTCCAGGTGG 
     3121 CCTCGAAATC TGTGCTGGCC GCACTCTACA GCTCAGAGGG CTCTGTCATG GAGAGGCACC 
     3181 ACTTTGCTCA AGCCATTGCT ATCCTCAACA CCCACGGCTG CAATATCTTT GACCACTTCT 
     3241 CTCGGAAGGA CTATCAGCGC ATGCTGGACC TGATGAGGGA CATCATCTTG GCTACAGACC 
     3301 TGGCACACCA CCTCCGCATC TTCAAGGACC TGCAGAAGAT GGCTGAAGTG GGTTATGACC 
     3361 GAAACAACAG GCAACACCAC AGGCTTCTTC TGTGCCTCCT CATGACCTCC TGTGACCTCT 
     3421 CTGACCAGAC AAAGGGCTGG AAGACCACCA GAAAGATTGC AGAGCTGATC TACAAAGAGT 
     3481 TCTTCTCCCA GGGAGACTTG GAGAAGGCCA TGGGCAACCG ACCGATGGAG ATGATGGACC 
     3541 GTGAGAAGGC CTACATCCCT GAGCTTCAGA TCAGCTTTAT GGAGCACATT GCCATGCCTA 
     3601 TCTACAAGCT TTTACAAGAC CTGTTCCCCA AGGCGGCAGA GCTGTATGAA CGTGTGGCCT 
     3661 CCAACCGTGA GCACTGGACC AAGGTGTCCC ACAAGTTCAC CATCCGAGGC CTCCCCAGTA 
     3721 ACAACTCGCT GGATTTCCTG GACGAGGAAT ACGAGGTCCC CGATTTGGAC GGCACCAGAG 
     3781 CTCCTGTCAA TGGCTGCTGC AGCCTCGAGG GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3841 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3901 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3961 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4021 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4081 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4141 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4201 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4261 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4321 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4381 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4441 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4501 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4561 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4621 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4681 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4741 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4801 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4861 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4921 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4981 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5041 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5101 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5161 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5221 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5281 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5341 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5401 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5461 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5521 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5581 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5641 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5701 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5761 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5821 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5881 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5941 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6001 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6061 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6121 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6181 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6241 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6301 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6361 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6421 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6481 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6541 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6601 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6661 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6721 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6781 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6841 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6901 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6961 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7021 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7081 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7141 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7201 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7261 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7321 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7381 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7441 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7501 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7561 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7621 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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