LOCUS       MR219032                7962 bp    DNA     circular  SYN 
DEFINITION  Myo1g (Myc-DDK-tagged) - Mouse myosin IG (Myo1g)
ACCESSION   NM_178440
VERSION     NM_178440.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4117..4208 
                     /label="Myc-DDK"  
     misc_feature    4238..4823 
                     /label="PolyA signal"  
     misc_feature    4295..4314 
                     /label="Restriction Enzyme"  
     misc_feature    5099..5771 
                     /label="ColE1"  
     misc_feature    6201..6995 
                     /label="Kan/Neo"  
     misc_feature    7030..7332 
                     /label="SV40 ori"  
     misc_feature    7418..7895 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4092..4099 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4092 
                     /label="Myo1g"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTGCAGTG GGCAGGATGG AGGACGAAGA AGGCCCTGAG TATGGGAAAC 
     1081 CAGACTTCGT GCTTTTGGAT CAACTGACCA TGGAGGACTT CATGAAGAAC CTAGAGCTCA 
     1141 GGTTTGAGAA GGGCCGTATC TATACCTACA TTGGTGAGGT GCTCGTATCC GTGAACCCCT 
     1201 ACCAGGAACT GCCATTGTAT GGGCCAGAGG CCATTGCCAA GTACCAGGGC CGCGAGCTCT 
     1261 ATGAGCGACC ACCTCATCTT TACGCCGTGG CCAATGCTGC TTACAAGGCA ATGAAGCGCA 
     1321 GATCCAGGGA CACCTGCATC GTCATCTCAG GGGAGAGTGG GGCAGGGAAG ACAGAAGCCA 
     1381 GCAAGCACAT CATGCAGTAC ATTGCTGCTG TCACCAACCC AAGCCAGAGG GCTGAGGTGG 
     1441 AGAGGGTGAA GAATGTGCTC CTCAAGTCCA CCTGTGTGCT CGAAGCCTTT GGCAATGCCC 
     1501 GCACCAATCG CAACCACAAC TCCAGCCGCT TTGGCAAGTA CATGGACATC AACTTCGACT 
     1561 TCAAGGGGGA TCCTGTTGGT GGACACATCC ACAGCTACCT GCTGGAGAAG TCTAGGGTTC 
     1621 TCAAGCAACA TGTAGGTGAG AGGAACTTCC ATGCCTTCTA CCAGTTGCTT CGAGGCAGTG 
     1681 AGGACCAAGA GCTGCAAGGA CTGCATCTGG AAAGAAATCC TGCTGTGTAT AATTTCACGC 
     1741 GTCAGGGAGC TGGGCTCAAC ATGGGTGTGC ACAATGCCTT GGACAGTGAT GAGAAGAGCC 
     1801 ACCAAGGAGT GATGGAGGCC ATGAGGATCA TCGGCTTCAG TCCTGACGAG GTGGAGTCCA 
     1861 TCCATCGCAT CCTTGCCGCC ATATTACACC TGGGAAACAT CGAGTTTGTG GAGACAGAGG 
     1921 AAAATGGACC ACAGAAAGGA GGCCTGGAAG TGGCTGATGA GGCCCTGGTA GGATATGTGG 
     1981 CCAAGCTGAC AGCCACTCCC AGAGACCTTG TTCTGCGAAC CCTGCTGGCT CGAACAGTGG 
     2041 CTTCAGGAGG CCGAGAAGTC ATTGAGAAGA GCCACACCGT GGCTGAGGCC AGCTATGCCC 
     2101 GGGATGCCTG TGCAAAGGCG ATGTACCAGC GACTGTTTGA GTGGGTTGTG AACAAGATCA 
     2161 ATAGCATCAT GGAACCCCGA AACCGAGACC CTCGGTGTGA TGGCAAGGAT ACTGTCATTG 
     2221 GGGTGCTGGA CATTTACGGC TTTGAGGTGT TCCCTGTCAA CAGCTTTGAG CAGTTCTGCA 
     2281 TCAACTACTG CAACGAGAAG CTGCAGCAGC TCTTTATCCA GCTTATCCTG AAGCAAGAGC 
     2341 AGGAGGAGTA TGAGCGAGAG GGCATCGCCT GGCAGACCAT CGAGTACTTC AACAACGCTA 
     2401 CCATTGTGGA ACTTGTAGAG CAGCCCCACA GAGGCATCCT GGCTGTGTTA GATGAAGCCT 
     2461 GCAGCACGGC AGGCCCCATC ACTGACCGAA TCTTCCTGCA GACCCTGGAC ACACACCACC 
     2521 GCCACCACCC ACACTATTCC AGCCGCCAGC TTTGCCCTAC GGACAAGACC ATGGAGTTTG 
     2581 GCCGAGACTT CCAGATCAAA CACTATGCAG GCGATGTCAC GTACTCTGTG GAAGGCTTCA 
     2641 TTGACAAGAA TAGAGACTCT CTCTTCCAGG ACTTCAAACG GCTGCTGTAC AATAGTGTGG 
     2701 ATCCCACCTT GCGAGCCATG TGGCCTGACG GGCAACAGGA CATCACGGAA GTGACCAAGC 
     2761 GTCCCCTGAC AGCCGGCACA CTCTTTAAGA ATTCCATGGT TGCCCTGGTG GAAAACTTGG 
     2821 CTTCCAAGGA ACCCTTCTAT GTCCGCTGCA TCAAACCCAA CGAAGACAAG GTGGCTGGGC 
     2881 GGCTCGATGA AGCCCACTGT CGTCACCAGG TCGAATACCT GGGACTATTG GAGAATGTGA 
     2941 GGGTCCGCAG GGCTGGCTTT GCTTCCCGGC AGCCCTACCC TCGATTCCTG CTCAGGTACA 
     3001 AGATGACCTG TGAGTACACG TGGCCCAACC ACCTGCTGGG CTCTGACCGG GATGCGGTGA 
     3061 GCGCCCTGCT GGAACAGCAT GGGCTGCAAG GGGATGTGGC CTTTGGCCAC AGCAAGCTGT 
     3121 TCATCCGATC CCCAAGGACG CTGGTCACTC TGGAGCAGAG CCGAGCTCGC CTGATTCCCA 
     3181 TCATTGTGTT ATTGCTGCAG AAGGCTTGGC GGGGCACCCT GGCTAGGTGG CACTGCCGGC 
     3241 GACTAAGGGC CATCTACACC ATCATGCGCT GGTTCCGGAG GCACAAGGTG CGTGCTCACC 
     3301 TGATTGAACT ACAGCGCCGG TTCCAGGCTG CACGGCAGCC CCCACTCTAT GGCCGTGACC 
     3361 TTGTGTGGCC CACACCTCCT GCTGTGCTGC AGCCCTTCCA GGACACTTGC CGTGTTCTCT 
     3421 TCAGCAGGTG GCGGGCACGG CAGTTAGTGA AGAACATCCC TCCTTCAGAC ATGATCCAGA 
     3481 TCAAGGCCAA GGTGGCTGCT ATGGGGGCCT TGCAAGGATT GCGGCAGGAC TGGGGTTGCC 
     3541 AGCGGGCCTG GGCCCGAGAC TACCTGTCCT CTGACACTGA CAACCCCACA GCTTCCCATC 
     3601 TGTTTGCTGA GCAACTAAAG GCACTTCGGG AGAAAGATGG CTTTGGCTCT GTGCTTTTCT 
     3661 CCAGCCATGT GCGCAAGGTG AATCGCTTCC GCAAGAGCCG GGACCGGGCC CTTCTGCTCA 
     3721 CAGATCGGTA TCTGTACAAG CTGGAGCCTG GACGACAGTA CCGGGTGATG CGGGCTGTGC 
     3781 CTCTGGAGGC GGTGACAGGG CTGAGTGTGA CCAGTGGAAG AGATCAGCTG GTGGTGTTAC 
     3841 ATGCCCAAGG CTATGATGAT CTTGTAGTGT GTCTACACCG TTCCCAACCA CCACTGGACA 
     3901 ATCGAATTGG GGAGCTGGTG GGCATGCTGG CTGCACACTG CCAGGGAGAG GGACGAACTC 
     3961 TGGAGGTCCG TGTCTCTGAC TGCATCCCAC TGAGCCAGCG TGGTGCCCGG CGCCTCATTT 
     4021 CTGTGGAGCC CAGGCCAGAG CAGCCTGAGC CAGATTTCCA AAGCAGCCGT AGCACCTTTA 
     4081 CCCTCCTCTG GCCAAGCCAC AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4141 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4201 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4261 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4321 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4381 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4441 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4501 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4561 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4621 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4681 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4741 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4801 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4861 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4921 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4981 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5041 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5101 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5161 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5221 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5281 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5341 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5401 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5461 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5521 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5581 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5641 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5701 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5761 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5821 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5881 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5941 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6001 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6061 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6121 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6181 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6241 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6301 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6361 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6421 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6481 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6541 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6601 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6661 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6721 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6781 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6841 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6901 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6961 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7021 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7081 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7141 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7201 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7261 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7321 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7381 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7441 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7501 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7561 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7621 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7681 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7741 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7801 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7861 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7921 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//