LOCUS       MR219008                7908 bp    DNA     circular  SYN 
DEFINITION  Grid1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, delta 1 (Grid1)
ACCESSION   NM_008166
VERSION     NM_008166.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4065..4156 
                     /label="Myc-DDK"  
     misc_feature    4186..4771 
                     /label="PolyA signal"  
     misc_feature    4243..4262 
                     /label="Restriction Enzyme"  
     misc_feature    5047..5719 
                     /label="ColE1"  
     misc_feature    6149..6943 
                     /label="Kan/Neo"  
     misc_feature    6978..7280 
                     /label="SV40 ori"  
     misc_feature    7366..7843 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4047..4053 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4047 
                     /label="Grid1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGCGTTG ACGCTGTGGC TTCTTCCCTG GATATGCCAG TGCGTTACGG 
     1081 TGCGGGCCGA CTCCATCATC CACATCGGTG CCATCTTCGA GGAGAACGCA GCCAAGGACG 
     1141 ACAGGGTGTT CCAGTTGGCT GTATCAGACC TGAGCCTCAA TGATGACATC CTGCAGAGTG 
     1201 AGAAGATCAC CTACTCCATC AAGGTCATCG AGGCCAATAA TCCGTTCCAG GCGGTGCAAG 
     1261 AAGCCTGTGA CCTCATGACC CAGGGAATTT TGGCCTTGGT CACATCCACG GGCTGTGCAT 
     1321 CCGCCAATGC CCTGCAGTCC CTCACAGACG CCATGCACAT CCCACACCTC TTTGTCCAGC 
     1381 GCAACCCCGG GGGTTCACCA CGTACTGCCT GCCACCTGAA CCCTAGCCCT GACGGCGAGG 
     1441 CCTACACACT GGCTTCGAGA CCACCTGTCC GTCTCAATGA TGTCATGCTC AGGCTGGTGA 
     1501 CAGAACTGCG CTGGCAGAAG TTCGTCATGT TCTATGACAG CGAGTATGAT ATCCGTGGGC 
     1561 TCCAAAGCTT CCTGGATCAG GCCTCAAGGC TGGGTCTGGA TGTCTCTTTA CAAAAGGTGG 
     1621 ACAAGAACAT CAGTCACGTG TTCACTAGCC TTTTCACCAC CATGAAGACC GAGGAGCTGA 
     1681 ATCGCTACCG GGACACACTG CGCCGTGCCA TTCTACTGCT CAGCCCACAG GGGGCGCACT 
     1741 CCTTCATCAA TGAGGCTGTG GAGACCAACT TGGCTTCCAA GGACAGCCAC TGGGTCTTTG 
     1801 TGAATGAGGA AATCAGTGAC CCCGAGATCC TGGATCTGGT CCACAGTGCC CTTGGCAGGA 
     1861 TGACCGTGGT CCGGCAAATC TTCCCATCTG CAAAGGACAA CCAGAAATGT ATGAGGAATA 
     1921 ACCACCGCAT CTCCTCCCTG CTCTGTGATC CACAGGAAGG CTACCTCCAA ATGCTGCAGA 
     1981 TCTCCAATCT CTACCTGTAT GACAGTGTTC TGATGCTGGC CAACGCCTTC CACAGGAAGC 
     2041 TGGAAGACCG GAAGTGGCAT AGTATGGCAA GCCTTAACTG CATCCGGAAG TCTACCAAGC 
     2101 CATGGAATGG AGGGAGATCC ATGCTAGACA CGATTAAAAA GGGACATATC ACTGGCCTCA 
     2161 CAGGGGTTAT GGAGTTTCGG GAAGATAGCT CAAATCCTTA TGTCCAGTTT GAAATCCTTG 
     2221 GCACAACCTA TAGTGAGACT TTTGGCAAAG ACATGCGCAA GCTGGCCACC TGGGACTCGG 
     2281 AGAAGGGCTT GAATGGCAGT CTACAGGAGA GGCCCATGGG CAGCCGCCTC CAAGGACTGA 
     2341 CTCTCAAAGT GGTGACTGTC TTGGAAGAGC CTTTTGTGAT GGTAGCTGAG AATATCCTTG 
     2401 GACAACCCAA GCGTTACAAA GGGTTCTCCA TAGATGTGCT GGATGCACTG GCTAAGGCTC 
     2461 TAGGATTCAA ATATGAGATA TACCAGGCCC CTGATGGCAG GTATGGTCAC CAGCTCCATA 
     2521 ACACCTCCTG GAATGGGATG ATCGGGGAGC TCATTAGCAA GAGAGCAGAC TTGGCCATCT 
     2581 CTGCTATTAC CATCACCCCA GAGAGAGAGA GCGTTGTGGA CTTCAGCAAG CGCTACATGG 
     2641 ACTACTCAGT GGGGATCCTC ATCAAGAAGC CTGAGGAGAA GATCAGCATC TTTTCCCTTT 
     2701 TCGCCCCCTT TGACTTTGCG GTGTGGGCCT GCATTGCCGC AGCCATTCCT GTGGTGGGCG 
     2761 TGCTCATATT CGTGTTGAAT CGGATACAGG CTGTAAGATC TCAGAGTGCC ACCCAGCCAC 
     2821 GACCCTCGGC TTCTGCCACC CTGCACAGTG CCATTTGGAT TGTCTATGGA GCCTTTGTCC 
     2881 AGCAAGGTGG TGAGTCTTCG GTGAACTCTG TGGCCATGCG CATCGTGATG GGCAGCTGGT 
     2941 GGCTCTTCAC CCTCATTGTG TGTTCCTCCT ACACAGCCAA CCTGGCCGCT TTCCTCACAG 
     3001 TGTCTAGGAT GGACAACCCC ATAAGGACAT TTCAGGACCT GTCCAAGCAA CTGGAGATGT 
     3061 CTTATGGCAC TGTCCGGGAC TCTGCTGTCT ATGAGTACTT CAGAGCCAAG GGGACCAATC 
     3121 CCCTGGAGCA GGATAGCACT TTTGCTGAGC TCTGGCGGAC CATAAGCAAG AATGGAGGGG 
     3181 CTGACAACTG TGTGTCCAAT CCTTCAGAAG GTATCAGGAA GGCAAAGAAG GGGAACTACG 
     3241 CCTTTCTATG GGATGTGGCC GTGGTGGAGT ATGCAGCCCT GACAGATGAC GACTGCTCAG 
     3301 TGACTGTCAT CGGCAACAGC ATCAGCAGCA AGGGCTACGG GATTGCCCTG CAACATGGCA 
     3361 GCCCCTACAG GGACCTTTTC TCCCAGAGGA TCCTGGAGCT GCAAGACACA GGGGACCTGG 
     3421 ATGTGCTCAA GCAGAAGTGG TGGCCACACA CAGGTCGATG TGACCTCACC AGCCATTCCA 
     3481 GTACCCAGAC TGAAGGCAAA TCCCTTAAGC TGCACAGCTT CGCTGGGGTC TTCTGCATTT 
     3541 TGGCCATTGG CCTCCTTCTT GCCTGCCTCG TGGCTGCTCT AGAGTTATGG TGGAACAGCA 
     3601 ACCGGTGCCA CCAGGAGACC CCCAAAGAGG ACAAAGAAGT GAATCTGGAG CAGGTACACC 
     3661 GGCGCATCAA CAGCCTCATG GATGAAGACA TTGCTCACAA GCAAATTTCC CCAGCGTCCA 
     3721 TTGAGCTTTC TGCCCTAGAG ATGGGGGGCC TGGCTCCAAG CCAAGCTTTG GAGCCCACGA 
     3781 GGGAGTACCA GAACACCCAG CTCTCAGTCA GCACCTTTCT GCCGGAGCAG AGCAGCCATG 
     3841 GCACCAGCCG GACACTGTCG TCAGGGCCCA GCAGCAACCT GCCACTGCCA CTGAGCAGCT 
     3901 CAGCCACTAT GCCCTCAATT CAGTGCAAAC ACAGGTCGCC CAATGGGGGA CTGTTTCGAC 
     3961 AGAGCCCGGT GAAGACCCCC ATCCCTATGT CCTTCCAGCC CGTGCCTGGA GGCGTCCTTC 
     4021 CAGAGGCTCT GGACACCTCT CATGGCACCT CCATCACGCG TACGCGGCCG CTCGAGCAGA 
     4081 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4141 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4201 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4261 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4321 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4381 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4441 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4501 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4561 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4621 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4681 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4741 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4801 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4861 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4921 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4981 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5041 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5101 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5161 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5221 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5281 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5341 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5401 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5461 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5521 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5581 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5641 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5701 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5761 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5821 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5881 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5941 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6001 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6061 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6121 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6181 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6241 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6301 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6361 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6421 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6481 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6541 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6601 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6661 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6721 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6781 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6841 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6901 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6961 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7021 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7081 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7141 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7261 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7321 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7381 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7441 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7501 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7561 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7621 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7681 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7741 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7801 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7861 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//