LOCUS       MR218938                8583 bp    DNA     circular  SYN 
DEFINITION  Alpk1 (Myc-DDK-tagged) - Mouse alpha-kinase 1 (Alpk1)
ACCESSION   NM_027808
VERSION     NM_027808.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4738..4829 
                     /label="Myc-DDK"  
     misc_feature    4859..5444 
                     /label="PolyA signal"  
     misc_feature    4916..4935 
                     /label="Restriction Enzyme"  
     misc_feature    5720..6392 
                     /label="ColE1"  
     misc_feature    6822..7616 
                     /label="Kan/Neo"  
     misc_feature    7651..7953 
                     /label="SV40 ori"  
     misc_feature    8039..8516 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4713..4720 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4713 
                     /label="Alpk1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATAATCAA GATGCCGTGG CTTCGATACT GCATGAGTGC AAGCAAGTGC 
     1081 TGGATCGTCT CTTGTTGGAA ACGCCAGATG TGTCTACAGA GGACAAGAGT GAGGACCAGC 
     1141 GGTGCAGAGC TTCACTCCCC AGCGAGTTAA GGACCCTGAT CCAGGAGGCA GAGGAAATGA 
     1201 AGTGGCCCTT CGTGCCTGAA AAGTGGCAAT ACAAACAAGC CATGAGCCCA GAGGACAAAA 
     1261 CAAACCTGCA AGATGTGATT GGCGCCGGGC TGCAGCAGTT ACTGGCGGCC TTGCGAGCCT 
     1321 CCATCCTTGT CCAGGACTGC GCTGCCGCCT CCGCTATTGT CTTCTTGATG GACCGGTTCC 
     1381 TGTATGGACT CGACGTCTCC GGAAAACTCC TGCAGGTCGC CAAAGGCTTG CACAAGCTGA 
     1441 AGCCTGCAAC GCCCATCGCC CCGCAGGTGG TCATCCGACA AGCCCGAGTC TCCGTCAACT 
     1501 CAGGGAAACT GTTGAAGGCA GAATACATCC TGAGCAGCCT GATAAGCAAC AACGGAGCAA 
     1561 CCGGGACCTG GCTGTATAGG AATGAGAGTG ACAAAGTCCT GGTACAGTCG GTCTGCATAC 
     1621 AGATCAGAGG GCAGATTCTG CAGAAGCTAG GCATGTGGTA TGAAGCGGCA GAATTGATAT 
     1681 GGGCCTCTGT CATAGGATAT CTGACACTCC CTCAGCCAGA TAAGAAGGGC ATTTCCACAT 
     1741 CACTGGGCAT ACTGGCAGAC ATCTTTGTTT CCATGAGCAA AACCGATTAT GAAAAGTTTA 
     1801 AGAAGAGCCC AAAGGTTAAC TTGGCCCTGC TCAAGGAGTT TGACCACCAT TTGTTGTCAG 
     1861 CCTCGGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA CACACCCCTC TTTGTGCTCA 
     1921 CGGCTGTGAA TATCCGTGGC ACATGTTTGT TGTCATATAG CTGTTCTGCT GACTGCCCTC 
     1981 CGGGAATGAA AAGTGTGCAT CTGTGTGAAG CCAAAGAAGC TTTTGAGATT GGCCTCCTGA 
     2041 CCAAGAAAGA TGGCGAGCTG GTTAGTGGCA AGCAGGAGCT GCACAGTTTC ATCAAAGCTG 
     2101 CTTTCGGCCT CACCACGGTG CACTCCAGAC TCCATGGGGA GACAGATGCA GTTCGCGCAG 
     2161 CAAGGCAGCT ATGCAGTGAA GCTGTGGGCA AGCTCTACAC CTTCAGCACT TCCCCCACAA 
     2221 GCCAGGACAG AGAAGGCCTG TCTCAGGAAA TCATGTCTCT CATCAGCCAG GTGAAGGGAC 
     2281 ATCTACGAGT TCAAAGCTTC CCAAATTTAG ATGCTTGCTC TTATGTCCCA GAGAGTTTCA 
     2341 AGTGTGGTTT AGATCGACTC ATCTTGCATG GACACGTGGA TTTCCAACAA ATTCTTGAAA 
     2401 CCTATTCCCA GCACCATACT TCAGTGTGTG AGGTATTTGA AAGCACTTGT GGGAACAGCA 
     2461 AACGCAACCA GAGAGACACA AAATCGGAGG TCTGTATTAC CACTCTGAAG ACAGAAACAA 
     2521 ACACCGCAGA TACTATGGTT GCTACTCTAG AGAGGGTGAG TTCCCAAGAC AGCAGGAGTA 
     2581 CAGCTTCTTC CAAAATGTCT AAGAAAGATC AAGGGAAGCT CCAAAGAGAA AGGGGGAGAA 
     2641 GCTGGACCCA TTCCAAGGCA TTCCGAGTCT CCCTGGATCT AGATATGGAG ACTGAGACTG 
     2701 AGCCACCAAA TCACAGCAAT GGAGGGACAG ATGTTTTCAA CAAGTCTCTG AGAGACAACT 
     2761 CTAGCTCCTG TTCTTGGGGC AGACTGTCAG GGCTCAGCTC TTCCACAAGC TGGGAGGAAG 
     2821 TGAACTGCGC TGTTCAGGAT GTAGTAAGGA AAGGGTCTGG CCAAGAAGAG CATCCTGTGG 
     2881 AGGCGCAAAG CTCTGAGGCA GCGTCTGAGG AGCCCAAGAG GAACAGAAGC AGCAGAGCTG 
     2941 TGTATTTGTC TTCAAAGCTC CGTGGTGTCT CTCTTCAGAC AACTGGAGAT GACAACTTAG 
     3001 AGTCTTCACC AAGTCAGTTA CACAATCACA CATCCATCCT TCCTTTCAAT GCAAAAGATA 
     3061 CTTGTTTGGC CTCGGGTGCA GGACTGGTAG AAACCGCTGA AGGATCTAGC AATACTTCTC 
     3121 TTCAGTCCAG TCATTCGTGT GGTTCTGATT CCTGGTCCTT GTCTAGTTCA GACAGGTTCA 
     3181 CGGATGTGAC CACGAATCCT TCAGTTCAGG AAGAAGAACC CTCTGGAATA ATGGGTGATG 
     3241 TCCCAGAATC CAAGTATGAC TGTAAAGACT GGCACGGAGA AAAGAATGGG GGAACACTCG 
     3301 CTGAAATATG CACAGGGCCT GAATTAACAT CTGCCCCCTC TTCGGTCGAC CCAGAAGGAG 
     3361 AAACAGCAGA AAGCACAGAC GATGGACTAT CACCCTCTCA GGTAGCCCTG GGATGTTTAG 
     3421 AAGGCAGCCA TTCAATGTCT ACGCGTAGGA CTTTCTTTCC GGATGGGTCG GTTCAAAACG 
     3481 CTGACTCAGC AAAGACTGGC TGCTCAGTCA GAGACCAGAC TGTCGACCCT GATGCCTCAA 
     3541 CGGTGGATGA GGAGGGCCAA ATGCTTGACA GCACAGAGAT CTGCTCCATT GGCCAAGATG 
     3601 GCGCTCACAG ACCACGTGCT TTGAGGTCCG GTCAGAGTGC GGAGGGGCCA AAGTCCTTTG 
     3661 TAAATGGCTC CAGTCCTTCC CCTATCTTCG ATGAGGACTT CAGCACCACA GAGGAAGGAG 
     3721 AGGAGCTTGG GAGCATGCTG AAGAGCAGCC AAAACTCCAG CTCATACTCA CCATGGTGGC 
     3781 TAAAATCACC AGCCTTTTCC CGCAGTTCGT CAGATGGGGA AAGCTCCTGG TCTCTGCTGA 
     3841 ACTCAAGCAG AAGTTCCTTC GCCTCGCTGG CAGGACAGAC CAGTCAAGAG ATCCTTGAGG 
     3901 CTCGAACCCT ACAGCCTGAT GACCTTGAAA AACTTCTGGC GGGCGTGAGG CATGATTGGC 
     3961 TGCTTCAGAG ACTGGAGAAT ACTGGCGTTT TGAAGTCCAA TCAACTCCAA CAAGCCCACA 
     4021 GTGCCCTTCT GCTAAAATAT TCCAAAAAGT CTGAGTTGTG GACAGCGCAG GAAACTGTGG 
     4081 TGTATCTGGG AGACTACCTG AAAGTGAAGA AGAAGGGCAA GCAGAGAAAT GCATTTTGGG 
     4141 TCCACTATCT CCATCAAGAG GAAACCCTGG GGAGATACGT TGGGAAAGAA TACAAAGAGA 
     4201 GGAAGGGGCT CCGGCACCAC TTCACTGATG TGGAGCGGCA GATGACAGCA CAGCACTACG 
     4261 TGACAGAGTT TAACAAGAGA CTCTACGAGC AAAAGATTCC GACTCAGATA TTCTACATCC 
     4321 CCTCTACAAT ATTACTGATT TTGGAAGACA GGACTATAAA GGGATGCATC AGTGTAGAGC 
     4381 CTTACATCCT GGGGGAATTC GTCAAGTTAT CCAATAACAC CAAAGCAGTC AAAAATGAGT 
     4441 ACAAAGCTAC AGAATATGGC TTGGCCTATG GCCATTTTTC TTATGAGTTT TCTAACCATA 
     4501 GAGATGTGGT GGTTGATTTA CAAGGTTGGG TGACTGGTAA TGGAAAAGGC CTGATCTATC 
     4561 TCACGGATCC TCAGATTCAC TCTGTAGACC AGAAAGATGT CACTACAAAC TTTGGAAAGC 
     4621 GGGGAATATT TTACTTCTTT AATAACCAAC ATGCAAGCTG TAATGAAATA TGCCATCGTC 
     4681 TTTCTCTGAC TAGACCTTCA CTAGAGCAAA CAAGTAAGGT AAGCGGACCG ACGCGTACGC 
     4741 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4801 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4861 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4921 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4981 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5041 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5101 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5161 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5221 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5281 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5341 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5401 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5461 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5521 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5581 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5641 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5701 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5761 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5821 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5881 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5941 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6001 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6061 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6121 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6181 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6241 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6301 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6361 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6421 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6481 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6541 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6601 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6661 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6721 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6781 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6841 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6901 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6961 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7021 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7081 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7141 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7201 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7261 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7321 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7381 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7441 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7501 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7561 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7621 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7681 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7741 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7801 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7981 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8041 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8101 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8161 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8221 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8281 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8341 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8401 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8461 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8521 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8581 TTT
//