LOCUS       MR218935                8166 bp    DNA     circular  SYN 
DEFINITION  Adcy2 (Myc-DDK-tagged) - Mouse adenylate cyclase 2 (Adcy2)
ACCESSION   NM_153534
VERSION     NM_153534.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4323..4414 
                     /label="Myc-DDK"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    4501..4520 
                     /label="Restriction Enzyme"  
     misc_feature    5305..5977 
                     /label="ColE1"  
     misc_feature    6407..7201 
                     /label="Kan/Neo"  
     misc_feature    7236..7538 
                     /label="SV40 ori"  
     misc_feature    7624..8101 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4305..4311 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4305 
                     /label="Adcy2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCAGGAG GCGATGCGGC GGCGCCGCTA CCTGCGGGAC CGCGCCGAGG 
     1081 CGGCGGCAGC AGCGGCGGCG GGCGGCGGCG AGGGGCTGCA GCGGTCCCGG GACTGGCTCT 
     1141 ACGAGTCCTA CTACTGCATG AGCCAGCAGC ACCCTCTCAT CGTCTTCCTG CTGCTCATCG 
     1201 TCATGGGCGC CTGCCTCGCC CTGCTCGCCG TCTTCTTCGC GCTCGGGCTG GAGGTAGAAG 
     1261 ACCATGTGGC ATTTTTAATA ACCGTTCCCA CTGCCCTGGC CATCTTCTTC GCCATATTCA 
     1321 TTCTTGTCTG CATTGAGTCT GTGTTCAAGA AGCTTCTCCG AGTGTTCTCG CTGGTGATCT 
     1381 GGATATGTCT GGTTGCCATG GGATACCTGT TCATGTGCTT TGGAGGGACC GTGTCTGCCT 
     1441 GGGACCAGGT GTCATTCTTC CTCTTCATCA TCTTCGTGGT GTACACCATG CTTCCCTTCA 
     1501 ACATGAGGGA TGCCATCATC GCCAGCGTCC TCACCTCTTC ATCTCATACG ATAGTGCTGA 
     1561 GTGTCTACCT GTCTGCAACA CCAGGGGCCA AGGAGCACCT GTTCTGGCAG ATACTGGCCA 
     1621 ACGTGATCAT TTTCATCTGT GGGAACTTGG CGGGAGCTTA CCATAAGCAC CTCATGGAAC 
     1681 TTGCCCTGCA GCAAACCTAT CGGGACACAT GTAATTGCAT CAAGTCCCGG ATCAAGCTGG 
     1741 AATTTGAAAA ACGGCAGCAG GAACGACTTC TGCTTTCCCT GCTGCCTGCT CACATTGCCA 
     1801 TGGAAATGAA GGCTGAAATC ATTCAAAGGC TACAGGGACC CAAAGCAGGA CAGATGGAAA 
     1861 ACACGAACAA CTTCCACAAC CTGTATGTCA AGCGACACAC CAACGTGAGC ATATTATATG 
     1921 CTGACATTGT TGGCTTCACC CGCCTTGCAA GCGATTGCTC CCCTGGTGAA CTGGTCCACA 
     1981 TGCTGAATGA ACTCTTTGGG AAGTTTGATC AAATTGCAAA GGAGAATGAG TGCATGAGAA 
     2041 TTAAAATTTT AGGAGACTGC TATTACTGTG TTTCCGGGCT CCCGATATCT CTCCCTAACC 
     2101 ATGCCAAGAA CTGTGTGAAA ATGGGACTGG ATATGTGCGA AGCCATAAAG AAAGTGAGGG 
     2161 ATGCTACTGG GGTCGATATC AACATGCGCG TGGGAGTGCA TTCTGGGAAC GTTCTCTGTG 
     2221 GTGTGATTGG TCTCCAGAAG TGGCAGTATG ACGTGTGGTC TCATGATGTT ACTTTGGCCA 
     2281 ACCACATGGA AGCTGGAGGA GTCCCTGGAC GTGTTCACAT TTCCTCAGTC ACTCTGGAGC 
     2341 ACTTGAATGG GGCTTATAAA GTGGAGGAAG GAGACGGTGA GATAAGAGAC CCATATTTAA 
     2401 AGCAGCATTT GGTGAAAACC TACTTTGTAA TCAACCCCAA GGGAGAAAGA CGGAGTCCTC 
     2461 AGCATCTCTT CAGACCTCGA CACACTCTGG ATGGAGCCAA GATGAGAGCA TCTGTCCGCA 
     2521 TGACCCGGTA CTTGGAGTCC TGGGGAGCAG CCAAGCCATT TGCACATCTG CACCACAGAG 
     2581 ATAGCATGAC CACAGAGAAT GGGAAGATTA GCACCACGGA TGTGCCAATG GGTCAACATA 
     2641 ATTTTCAAAA TCGCACCTTA AGAACTAAGT CACAGAAGAA GCGATTCGAA GAAGAACTGA 
     2701 ATGAAAGGAT GATCCAAGCA ATTGACGGGA TCAACGCACA AAAGCAATGG CTCAAGTCAG 
     2761 AAGACATTCA AAGAATCTCC CTGTTTTTCT ATAACAAAAA TATAGAAAAA GAATATCGAG 
     2821 CCACTGCACT GCCAGCATTC AAGTACTACG TGACCTGTGC CTGCCTCATC TTCCTCTGTA 
     2881 TCTTCATTGT ACAGATTCTT GTACTGCCAA AAACGTCCAT CCTTGGCTTC TCCTTTGGAG 
     2941 CTGCATTTCT CTCCCTCATC TTCATCCTCT TTGTCTGCTT CGCTGGACAG CTTTTGCAAT 
     3001 GCAGCAAAAA GGCCTCTGCC TCTCTCTTGT GGCTTTTGAA ATCATCAGGC ATCATAGCCA 
     3061 ACCGCCCATG GCCACGGATC TCCCTCACGA TCGTCACCAC AGCTATCATA CTAACCATGG 
     3121 CTGTGTTTAA CATGTTTTTC CTGAGCAACT CTGAGGAGAC AACCCTTCCG ACTGCCAATG 
     3181 CATCTAATGC AAACGTTTCT GTCCCAGACA ACCAGACAGC AATTCTTCAT GCACGAAACT 
     3241 TGTTTTTCCT CCCGTACTTC ATATACAGCT GCATTCTGGG TTTGATCTCC TGCTCCGTTT 
     3301 TCCTGAGGGT GAACTACGAG TTGAAAATGT TAATCATGAT GGTGGCACTC GTGGGCTACA 
     3361 ACATCATTCT GCTCCACACC CATGCCCATG TCCTGGATGC ATACAGCCAG GTCCTGTTTC 
     3421 AGAGACCAGG CATTTGGAAA GACCTGAAGA CCATGGGCTC TGTGTCCCTC TCTATATTCT 
     3481 TCATCACGCT GCTCGTTCTG GGTAGACAGA GTGAATATTA CTGTAGGTTA GACTTCTTGT 
     3541 GGAAGAACAA GTTCAAAAAA GAGCGGGAGG AGATCGAAAC CATGGAGAAC CTAAACCGAG 
     3601 TGCTGCTGGA GAACGTGCTA CCTGCACACG TGGCTGAACA CTTCCTGGCC AGGAGCCTGA 
     3661 AAAACGAGGA GTTGTACCAC CAGTCCTACG ATTGTGTCTG TGTCATGTTT GCCTCCATTC 
     3721 CGGACTTCAA GGAATTCTAC ACGGAGTCAG ATGTGAACAA GGAAGGCTTG GAATGCCTCC 
     3781 GGCTCCTGAA TGAGATCATC GCTGACTTTG ATGATCTGCT TTCCAAGCCG AAGTTCAGTG 
     3841 GCGTTGAAAA GATCAAGACC ATTGGTAGCA CATACATGGC AGCCACGGGT CTGAGTGCTG 
     3901 TACCCAGTCA GGAGCACGCC CAGGAACCCG AGCGTCAATA CATGCACATA GGCACCATGG 
     3961 TGGAGTTTGC ATACGCCCTG GTGGGGAAAC TGGATGCCAT CAATAAGCAC TCCTTCAACG 
     4021 ACTTCAAACT TCGAGTGGGT ATCAACCATG GACCTGTAAT AGCTGGCGTC ATAGGGGCTC 
     4081 AGAAGCCACA ATATGACATC TGGGGCAACA CTGTCAACGT CGCTAGTAGG ATGGACAGCA 
     4141 CCGGAGTCCT GGACAAAATA CAGGTGACTG AGGAGACAAG CCTCATCCTG CAGACGCTTG 
     4201 GCTACACGTG TACATGTCGA GGTATCATCA ACGTGAAGGG GAAAGGGGAC CTGAAGACGT 
     4261 ACTTTGTAAA CACCGAGATG TCACGGTCCC TTTCTCAGAG CAACTTGGCT TCCACGCGTA 
     4321 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4381 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4441 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4501 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4561 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4621 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4681 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4741 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4801 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4861 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4921 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4981 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5041 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5101 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5161 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5221 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5281 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5341 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5401 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5461 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5521 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5581 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5641 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5701 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5761 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5821 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5881 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5941 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6001 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6061 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6121 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6181 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6241 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6301 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6361 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6421 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6481 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6541 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6601 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6661 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6721 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6781 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6841 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6901 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6961 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7021 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7081 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7141 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7201 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7261 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7321 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7381 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7441 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7501 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7561 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7621 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7681 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7741 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7801 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7861 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7921 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7981 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8041 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8101 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8161 AATTTT
//