LOCUS       MR218890                7302 bp    DNA     circular  SYN 
DEFINITION  Plcd4 (Myc-DDK-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 2
ACCESSION   NM_148937
VERSION     NM_148937.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3459..3550 
                     /label="Myc-DDK"  
     misc_feature    3580..4165 
                     /label="PolyA signal"  
     misc_feature    3637..3656 
                     /label="Restriction Enzyme"  
     misc_feature    4441..5113 
                     /label="ColE1"  
     misc_feature    5543..6337 
                     /label="Kan/Neo"  
     misc_feature    6372..6674 
                     /label="SV40 ori"  
     misc_feature    6760..7237 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3441..3447 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3441 
                     /label="Plcd4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACATCTCAG ATTCAAGACC TACTAGCCAC TGATCAGGAC CTGCTGCTGA 
     1081 TGCAAGAAGG CACAATGATG CGCAAGGTGA GGACCAAAAG CTGGAAGAAG CTAAGATACT 
     1141 TCAGACTTCA GAATGACGGC ATGACAGTCT GGCATGGAAG TCAACCAGAA AGCATGCCCA 
     1201 AGCCCACTTT TTCGATCTCT GATGTGGAGA GGATACGTAA GGGGCAGGAT TCTGAGTTGT 
     1261 TGCGCTATCT GGTGGAGGAG TTTCCCCTGG AGCAAGGGTT CACCGTTGTC TTTCATGGGC 
     1321 GCCGCCCCAA CCTGGACCTA GTGGCCAACA GTGTTGAAGA GGCCCAGATT TGGATGCGAG 
     1381 GACTCCAGCT GTTGGTGGAT CTTGTTGCCA GCATGGACCA CCAGGAGCAA ATGGATCAAA 
     1441 TGTTGAATGA GTGGTTCCAG CAAGCAGACA GAAACCAGGA TGGCCGGATG AGTTTCAGAG 
     1501 AAGCTCAACG GCTGCTGCTT CTGATGAACG TGGAAATGGA TGAAGAGTAC GCCTTCAGTC 
     1561 TTTTCCAGGA GGCAGATGTT ACCCAGTCTG ATGATCTGGG GTCGGAAGAA TTTGTACAGT 
     1621 TCTATAAGGC CCTGACTAAG CGCACTGAGA TTGAAGAAAT ATTTGAGGAC TTCTCATCTG 
     1681 ACAAACAGAA GCTGACCTTG CTGGAATTTG TGGATTTCCT TAGAAAGGAA CAGAAAGAAA 
     1741 AAGACCATGC TCCTGACCTC GCTCTGGAAC TCATTGACCG CTATGAGCCT TCTGAGAATG 
     1801 GCCGATTGCT GCATGTGCTG AGCAAGGATG GCTTCCTCAA GTACCTCTGC TCGAAGGATG 
     1861 GAAACATCTT CAACAGTGAC TGCCTCCCTA TCTACCAGGA TATGACTCAG CCTCTGAGCC 
     1921 ACTACTACAT TAACTCTTCT CACAACACCT ACCTAGTGGG GGACCAGCTT TGCGGCCAGA 
     1981 GCAGCGTTGA AGGATACATA CGGGCCTTGA AACGGGGGTG CCGCTGTGTG GAGGTGGACA 
     2041 CGTGGGATGG ACCTGATGGA GAGCCTGTTG TTTATCATGG GCATACCCTG ACCTCTCGCA 
     2101 TCCTATTCAA AGATGTGTTG GCAACTCTCG CGCAATATGC CTTCCAGTCA TCAGACTACC 
     2161 CTCTCATCTT GTCTCTGGAA AACCACTGCA CCTGGGAACA GCAGAGGACC ATGGCACACC 
     2221 ATCTGACCGA GATCCTGGGA GAGCAGCTGC TGAGGAACAC CCTAGAGGGA TTGCTGGTTG 
     2281 ACAGCATGCC CTCGCCAGAG CAGCTTCGGG GGAAGATCTT GGTGAAAGGA AAGAAGTTAA 
     2341 GAACAATTGA AGTTGACAAG GAAGAAGAAG AAGAAGAAGA AGAAGAAGAG CTGGAGAAAG 
     2401 ATGAGGGGCC AGATCTGGAC CCAGCCTCCC CTGAGTTGGA CACTCAACCT CAGCCTGAGA 
     2461 CCCAGGGGCA GGCCGCTGGG AACAAGAAGG AGAGGAAGAA GAAGGTTATG AAGTGTCCGA 
     2521 TGTCTTGTCT GCTTATCTGT GGTCATGTTA TGGCCCAGGC TCCTAGTTCC ATTCCTGAGT 
     2581 CCATTCTCTT GTCAAAGCAG TTTCTTCTCC TGAGTTCCAC AACCATCATG TGCCCAGACC 
     2641 TATCTGCCCT GGTTGTCTAC TTGAGGACTG TCCCCTTCTG CAGCTTCACT CATTCCAAAG 
     2701 AAAACTACCA CATCTATGAC ATTTCATCCT TTTCTGAGTC CAAGGCCAAG AACCTCATCA 
     2761 AGGAGGCAGG CAACGAGTTT GTACAGCACA ATGCCAGACA GTTATGCCGC GTGTATCCCA 
     2821 GTGGTCTGAG GACAGATTCA TCCAACTTTA ACCCCCAGGA ACACTGGAAT GTAGGCTGCC 
     2881 AGATGGTGGC TATGAATATG CAGACTGCAG GAAGTGCAAT GGATATCTGT GATGGGCTCT 
     2941 TCCGCCAGAA TGGTGGCTCT GGCTATGTGC TGAAGCCAGA ATTCCTGAGA GATACCCAGA 
     3001 GTTCATTCAA TCCTGAGAGG CCAATTAGTC TTTATAAGGC CCAGATCCTT GTAGTCCAGG 
     3061 TGATCAGTGG CCAACAACTC CCCAAAGTGG ATAAAACCAA AGAGACAACC GTTGTGGATC 
     3121 CACTGGTGAA AGTAGAGCTC TATGGTGTTC CTGAAGACAC AAAGGAGCAA GAGACCAGCC 
     3181 ATGTGGAGAA CAATGGTATT AATCCATACT GGGGGGAGAC TTTCTATTTC CGGCTCCAGG 
     3241 TACCTGAACT TGCTATGTTG CGTTTTGTGG TAAAGGATTA TAGCAGGAAA TCCCGAAATA 
     3301 ACTTTATTGG CCAATACACC CTACCTTGGA CCTGTATGAA ACAAGGCTAC CGACATGTAT 
     3361 CCTTGCTCTC CAGAGATGGC ACTAGCCTCA ATCCAGCTTC CATCTTTGTA TACACCTGCA 
     3421 TGCAGGAAGA CCTGGATATG GATGAGCCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3481 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3541 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3601 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3661 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3721 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3781 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3841 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3901 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3961 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4021 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4081 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4141 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4201 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4261 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4321 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4381 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4441 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4501 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4561 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4621 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4681 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4741 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4801 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4861 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4921 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4981 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5041 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5101 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5161 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5221 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5281 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5341 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5401 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5461 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5521 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5581 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5641 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5701 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5761 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5821 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5881 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5941 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6001 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6061 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6121 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6181 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6241 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6301 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6361 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6421 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6481 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6541 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6601 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6661 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6721 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6781 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6841 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6901 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6961 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7021 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7081 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7141 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7201 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7261 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//