LOCUS       MR218857                7437 bp    DNA     circular  SYN 
DEFINITION  Polk (Myc-DDK-tagged) - Mouse polymerase (DNA directed), kappa (Polk)
ACCESSION   NM_012048
VERSION     NM_012048.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3594..3685 
                     /label="Myc-DDK"  
     misc_feature    3715..4300 
                     /label="PolyA signal"  
     misc_feature    3772..3791 
                     /label="Restriction Enzyme"  
     misc_feature    4576..5248 
                     /label="ColE1"  
     misc_feature    5678..6472 
                     /label="Kan/Neo"  
     misc_feature    6507..6809 
                     /label="SV40 ori"  
     misc_feature    6895..7372 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3576..3582 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3576 
                     /label="Polk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATAACACA AAGGAAAAGG ACAACTTCAA AGACGACCTC CTGCTCCGCA 
     1081 TGGGACTAAA CGATAACAAA GCAGGCATGG AAGGGTTGGA TAAGGAGAAA ATTAACAAAA 
     1141 TTATCATGGA AGCCACAAAG GGGTCCAGAT TTTATGGAAA TGAGCTCAAG AAGGAAAAGC 
     1201 AAGTCAATCA ACGGATTGAA AATATGATGC AACAAAAAGC TCAAATTACC AGCCAGCAAC 
     1261 TAAGGAAAGC TCAATTACAG GTTGACAAAT TTGCAATGGA GTTAGAACGG AACCGGAATT 
     1321 TGAACAATAC CATAGTTCAT GTTGACATGG ACGCTTTCTA TGCAGCTGTG GAAATGAGGG 
     1381 ACAACCCGGA ACTGAAGGAT AAACCCATTG CTGTAGGATC CATGAGCATG TTGGCTACTT 
     1441 CGAATTACCA TGCAAGGAGG TTTGGTGTCC GTGCAGCCAT GCCAGGATTT ATTGCTAAGA 
     1501 GGCTCTGCCC ACAACTTATT ATAGTGCCCC CAAACTTTGA CAAATATAGA GCTGTGAGTA 
     1561 AGGAGGTTAA GGAGATACTT GCTGAATATG ATCCCAATTT TATGGCCATG AGTCTGGACG 
     1621 AAGCCTACTT GAATATAACA CAGCACTTGC AGGAAAGGCA AGATTGGCCT GAGGACAAAA 
     1681 GAAGATACTT CATCAAGATG GGAAACTACT TAAAAATCGA CACACCCAGA CAGGAAGCTA 
     1741 ACGAGCTGAC TGAGTATGAG CGGTCCATCT CCCCGCTGCT TTTTGAAGAT AGTCCTCCTG 
     1801 ATTTGCAACC CCAAGGAAGT CCTTTCCAAC TGAACTCTGA AGAACAAAAC AATCCTCAAA 
     1861 TAGCCCAAAA TTCAGTTGTT TTTGGAACAT CAGCTGAGGA AGTGGTAAAG GAAATTCGCT 
     1921 TCAGAATTGA ACAAAAAACA ACGCTGACAG CCAGCGCAGG CATCGCCCCC AATACAATGT 
     1981 TAGCAAAAGT GTGCAGTGAT AAGAATAAGC CAAACGGACA GTACCAGATC CTTCCCAGCA 
     2041 GGAGCGCGGT GATGGACTTC ATCAAGGACC TGCCTATTAG AAAGGTTTCT GGGATAGGAA 
     2101 AAGTTACAGA GAAAATGTTA ATGGCTCTCG GGATTGTTAC TTGCACAGAA CTCTACCAAC 
     2161 AGAGAGCGTT GCTGTCTCTC CTTTTCTCTG AAACCTCTTG GCATTATTTT CTTCACATCG 
     2221 CGCTGGGTCT AGGTTCAACA GACCTGGCAA GGGATGGAGA AAGGAAAAGC ATGAGTGTTG 
     2281 AAAGGACATT CAGTGAGATA AGTAAGACAG AGGAACAGTA CAGCCTGTGC CAAGAACTGT 
     2341 GCGCTGAGCT CGCCCACGAC CTCCAGAAGG AAGGACTTAA GGGAAGAACC GTCACCATTA 
     2401 AGCTGAAGAA CGTGAATTTT GAAGTAAAAA CTCGTGCATC TACCGTTCCG GCCGCCATTT 
     2461 CTACTGCAGA GGAAATATTT GCCATTGCCA AGGAGCTGCT AAGGACAGAA GTTAATGTGG 
     2521 GTTCTCCACA CCCCCTGCGG TTAAGACTGA TGGGTGTCCG AATGTCTACT TTTTCCAGTG 
     2581 AAGATGACAG GAAACACCAA CAAAGGAGCA TCATTGGTTT CTTACAAGCT GGAAACCAAG 
     2641 CTTTGTCATC TACTGGGGAT AGTCTAGACA AAACTGACAA AACTGAGCTT GCAAAGCCCT 
     2701 TAGAAATGTC TCATAAGAAG AGTTTCTTTG ATAAAAAGCG ATCAGAAAGA ATCTCCAACT 
     2761 GTCAAGACAC ATCCAGATGT AAAACTGCGG GTCAGCAAGC TTTACAGATC TTGGAACCAT 
     2821 CCCAAGCATT AAAGAAGCTG AGCGAGAGTT TTGAAACATC AGAGAATTCA AATGACTGTC 
     2881 AGACATTTAT ATGTCCAGTT TGCTTTAGGG AGCAAGAAGG TGTCAGTCTG GAAGCCTTTA 
     2941 ATGAACATGT AGATGAGTGT CTTGATGGAC CGTCAACCAG TGAGAACTCA AAAATATCCT 
     3001 GTTACTCACA TGCTTCCTCT GCAGACATTG GTCAGAAGGA AGATGTACAC CCCTCTATTC 
     3061 CACTGTGTGA GAAACGGGGG CATGAAAATG GAGAGATCAC TTTAGTAGAT GGTGTAGATC 
     3121 TAACAGGGAC GGAAGACAGA TCATTGAAAG CAGCAAGGAT GGACACTCTA GAGAATAATC 
     3181 GCAGCAAAGA GGAATGTCCT GATATTCCAG ACAAGTCTTG TCCTATATCA CTGGAAAATG 
     3241 AAACCATCAG TACATTAAGT AGGCAAGACT CTGTCCAGCC TTGTACAGAT GAGGTAGTAA 
     3301 CAGGACGAGC TCTAGTGTGT CCTGTTTGTA ACCTAGAACA AGAGACTTCT GATCTTACCC 
     3361 TCTTCAACAT ACATGTGGAT ATTTGCTTAA ATAAAGGTAT TATCCAAGAA CTGAGAAATA 
     3421 GTGAAGGTAA TTCAGTTAAA CAACCCAAAG AAAGCTCGAG AAGTACTGAC AGACTTCAGA 
     3481 AGGCTTCAGG AAGGACCAAA AGGCCAGGAA CGAAGACAAA GAGCTCAACT TTGAAGAAAA 
     3541 CAAAGCCCCG AGATCCCAGA CACACCCTTG ATGGATTTTT TAAAACGCGT ACGCGGCCGC 
     3601 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3661 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3721 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3781 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3841 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3901 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3961 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4021 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4081 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4141 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4201 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4261 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4321 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4381 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4441 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4501 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4561 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4621 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4681 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4741 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4801 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4861 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4921 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4981 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5041 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5101 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5161 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5221 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5281 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5341 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5401 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5461 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5521 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5581 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5641 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5701 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5761 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5821 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5881 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5941 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6001 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6061 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6121 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6181 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6241 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6301 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6361 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6421 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6481 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6541 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6601 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6661 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6721 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6781 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6841 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6901 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6961 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7021 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7081 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7141 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7201 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7261 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7321 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7381 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//