LOCUS       MR218828                8640 bp    DNA     circular  SYN 
DEFINITION  Mtr (Myc-DDK-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr)
ACCESSION   NM_001081128
VERSION     NM_001081128.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="Mtr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAAAACC CTGCAGGATG AGATTGAAGC CATTCTTCGG AAGAGGATCA 
     1081 TGGTGCTAGA CGGTGGGATG GGGACCATGA TCCAGCGGTA CAAACTAAGT GAAGAACATT 
     1141 TCCAAGGTCA AGAGTTTAAA GATCACAGCA GGCCACTGAA AGGCAACAAT GACATCTTAA 
     1201 GTATAACTCA GCCTGATATT ATTTACCAGA TTCATAAGGA ATACTTGCTG GCTGGAGCAG 
     1261 ATATCATCGA AACAAACACT TTCAGCAGTA CTAGTATCGC CCAGGCTGAC TATGGCCTTG 
     1321 AACACTTGGC CTACCGGATG AACAAGTGCT CTGCAGATGT GGCCAGAAAA GCCGCTGAGG 
     1381 AGATAACTCT GCAGACAGGA GTCAAGAGGT TTGTGGCTGG AGCTCTGGGT CCGACTAATA 
     1441 AGACACTTTC TGTCTCCCCA TCTGTGGAAA GGCCAGATTA TAGGAACATC ACATTTGATG 
     1501 AGCTTGTTGA CGCATACCAG GAGCAGGCCA AGGGACTGCT GGATGGCAGG GTCGACATCT 
     1561 TACTCATTGA AACAATTTTT GATACAGCTA ATGCCAAAGC AGCCTTGTTT GCGATCCAGA 
     1621 ACCTCTTTGA AGAGAATTAT GCTCCTCCTC GGCCTATCTT TATTTCTGGG ACCATTGTTG 
     1681 ATAAAAGTGG AAGGACTCTT TCTGGGCAGA CGGGAGAGGC TTTTGTCACC AGCGTGTCTC 
     1741 ATTCGGACCC CCTGTGCATT GGATTAAATT GTTCTCTGGG TGCAGCTGAA ATGAGGCCTT 
     1801 TCATTGAAAC AATTGGAAAA TGTACAACAG CCTATGTCCT CTGTTACCCA AATGCAGGTC 
     1861 TTCCCAACAC TTTTGGCGAC TATGATGAAA CACCATCCAC GATGGCCACG CACCTGAAGG 
     1921 ACTTTGCTGT GGATGGCTTG GTGAATATAG TTGGTGGATG CTGTGGTTCA ACACCTGATC 
     1981 ACATCAGGGA AATTGCGGAA GCTGTGAAAA AGTGTAAGCC AAGAGTCCCC CCTGCTAGTG 
     2041 TTTTTGAAGG ACATATGTTA CTGTCTGGTC TAGAGCCCTT CAGGATTGGA CCATACACCA 
     2101 ACTTTGTTAA CATTGGAGAG CGCTGTAATG TGGCAGGATC CAGGAAATTT GCTAAACTCA 
     2161 TCATGGCAGG AAACTATGAA GAAGCCCTGA GCATTGCCAA AGCACAGGTG GAAATGGGAG 
     2221 CTCAGGTGCT CGATATCAAC ATGGACGACG GCATGCTGGA TGGTCCCAGT GCAATGACCA 
     2281 GATTTTGCAA CTCCATTGCT TCTGAGCCTG ACATTGCCAA GGTGCCCCTG TGCATTGACT 
     2341 CTTCTAACTT TGCCGTGATT GAAGCTGGGT TGAAGTGCTG CCAAGGGAAG TGCATAGTCA 
     2401 ACAGCATCAG CCTCAAGGAG GGGGAGGGGG ACTTCCTGGA GAAGGCCCGG AAGATTAAGA 
     2461 AGTTTGGAGC TGCTGTGGTG GTTATGGCTT TTGATGAGGA AGGACAAGCA ACAGAAACAG 
     2521 ATGTTAAAGT CAATGTGTGC ACCAGAGCTT ACCATCTACT TGTGGACAAA GTGGGCTTCA 
     2581 ATCCAAATGA CATCATCTTT GACCCCAACA TCCTTACCAT TGGTACTGGA ATGGAGGAAC 
     2641 ATAACTTGTA TGCTATCAAT TTTATCCATG CAACAAGAGT CATTAAGGAA ACCTTACCAG 
     2701 GAGTCAGAAT AAGTGGAGGT CTTTCCAACT TATCCTTCTC CTTCCGAGGG ATGGAAGCCA 
     2761 TTCGAGAAGC AATGCATGGT GTGTTCCTTT ATCACGCGAT CAAGTTTGGT ATGGACATGG 
     2821 GGATAGTCAA TGCCGGCAAC CTCCCTGTGT ATGATGCTAT CCACAAGGAC CTTCTTCAGC 
     2881 TCTGTGAAGA CCTCATCTGG AACAAAGACT CTGAGGCTAC TGAGAAACTC TTGCGATATG 
     2941 CCCAGACTCA TGGCACAGGA GGGAAGAAAG TCATCCAGAC AGATGAGTGG AGGAATGGCT 
     3001 CCATTGAAGA GCGCCTGGAG TATGCTCTTG TGAAGGGCAT TGAAAAGCAC ATTGTTGAAG 
     3061 ATACTGAAGA AGCCAGGTTA AACGGGGAAA AATACCCTCG GCCTCTGAAT ATAATTGAAG 
     3121 GGCCCCTAAT GAATGGCATG AAAGTGGTTG GTGATCTTTT TGGAGCTGGA AAGATGTTTC 
     3181 TACCTCAGGT TATAAAGTCA GCCCGTGTCA TGAAGAAGGC TGTTGGCCAC CTTATCCCTT 
     3241 TCATGGAAAA AGAAAGAGAA GAAGCCAGAT TGATTAATGG TTCGGTTGAA GAAGAGGACC 
     3301 CTTACCAAGG CACCATTGTG CTGGCCACTG TTAAAGGGGA CGTGCATGAC ATCGGCAAGA 
     3361 ACATAGTTGG CGTGGTGCTG GCCTGTAACA ATTTCAGAGT TATTGATTTA GGAGTCATGA 
     3421 CTCCCTGTGA TAAGATACTT CAAGCTGCTC TGGACCACAA AGCAGATATA ATTGGCTTGT 
     3481 CAGGACTCAT CACTCCATCC TTGGATGAGA TGATTTTTGT TGCCAAGGAA ATGGAGAGGT 
     3541 TGGCTATAAA GATTCCATTG TTGATAGGAG GAGCAACCAC TTCAAGAACC CACACAGCAG 
     3601 TTAAAATTGC ACCACGGTAC AGCGCGCCTG TGATCCACGT CCTAGATGCA TCCAAGAGTG 
     3661 TGGTGGTGTG TTCTCAGCTG TTAGATGAAA ATCTGAGAGA TGACTACTTT GAAGAAATAC 
     3721 TGGAAGAGTA TGAAGATATT AGACAGGACC ACTATGAGTC TCTCAAAGAG AGAAAATACG 
     3781 TACCCCTAAG TCAAGCCAGA AAACACGGTT TCCACATTGA TTGGCTCTCT GAACCTCATC 
     3841 CAGTGAAGCC CACGTTTATT GGGACCCAGG TCTTTGAGGA CTACAACCTG CAAAAGCTGG 
     3901 TGGACTACAT TGACTGGAAG CCTTTCTTTG ACGTCTGGCA GCTCCGGGGC AAGTACCCGA 
     3961 ACCGAGGATT TCCCAAGATA TTTAATGACA AAGCAGTAGG TGAAGAAGCC AGAAAGGTAT 
     4021 ACAATGATGC TCAGAATATG CTGAACATAC TGATTAGTCA AAAGAAACTG CAGGCCAGGG 
     4081 GTGTGGTTGG ATTCTGGCCA GCACAGAGTG TCCAGGATGA CATCCACCTG TATGCAGAGG 
     4141 GGGTGGTGCC CCAGGCCGCT GAGCCCATAG CCACCTTCTA TGGACTGAGG CAGCAGGCTG 
     4201 AGAAGGACTC TTCTAGTACA GACCCCTACC ACTGCCTCTC AGACTTCATT GCTCCTTTGC 
     4261 ATTCTGGTGT CTGTGACTAC TTGGGTCTGT TTGCTGTTGC CTGCTTTGGG GTTGAAGAGC 
     4321 TGAGTAAGAC CTATGAGGAT GATGGCGATG ACTACAGCAG CATCATGGTG AAGGCGCTGG 
     4381 GCGACAGGCT GGCAGAGGCC TTTGCAGAGG AGCTCCATGA GAGAGTTCGC CGAGAACTGT 
     4441 GGGCCTACTC TAGGAGTGAG CAGTTGGGCG TCCCAGACTT GCGCAGACTC CGGTATGAGG 
     4501 GCATCCGGCC AGCTCCTGGT TACCCCAGCC AGCCTGACCA TACTGAGAAG CTTACCATGT 
     4561 GGAGACTGGC CAGCATCGAG CAGGCCACAG GCATCAGGCT GACAGAATCC TTGGCAATGG 
     4621 CACCCGCTTC AGCAGTGTCA GGGCTGTACT TCTCCAACGT AAAGGCCAAA TATTTTGCTG 
     4681 TGGGGAAAAT TTCCAAAGAT CAGACTGAGG ATTATGCTTT GAGGAAGAAC ATGCCAGTGG 
     4741 CCGAGGTGGA GAAATGGCTT GGTCCCATTC TGGGCTATGA CACAGACACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//