LOCUS       MR218816                8667 bp    DNA     circular  SYN 
DEFINITION  Shank2 (Myc-DDK-tagged) - Mouse SH3/ankyrin domain gene 2 (Shank2), transcript variant 1
ACCESSION   NM_001081370
VERSION     NM_001081370.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4824..4915 
                     /label="Myc-DDK"  
     misc_feature    4945..5530 
                     /label="PolyA signal"  
     misc_feature    5002..5021 
                     /label="Restriction Enzyme"  
     misc_feature    5806..6478 
                     /label="ColE1"  
     misc_feature    6908..7702 
                     /label="Kan/Neo"  
     misc_feature    7737..8039 
                     /label="SV40 ori"  
     misc_feature    8125..8602 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4806..4812 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4806 
                     /label="Shank2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAGCGTC CCCGGCGGTG GAGCAGCCAC CGTGATGATG ACCGGTTACA 
     1081 ATAATGGTCG CTATCCCCGG AATTCTCTCT ACAGTGACTG CATTATTGAG GACAAGACGG 
     1141 TGGTCCTGCA AAAGAAGGAC AACGAGGGCT TTGGATTTGT GCTCCGAGGG GCAAAAGCCG 
     1201 ATACCCCCAT TGAGGAATTC ACACCCACGC CAGCATTTCC AGCCCTGCAG TACCTGGAGT 
     1261 CCGTGGATGA AGGTGGGGTG GCATGGCAAG CCGGACTAAG GACCGGGGAC TTCTTGATTG 
     1321 AGGTTAACAA TGAAAATGTC GTCAAGGTGG GCCACAGGCA GGTGGTGAAC ATGATCCGCC 
     1381 AGGGAGGGAA TCACCTCATC CTTAAGGTCG TCACGGTGAC CAGGAATCTA GACCCTGATG 
     1441 ATACAGCCAG AAAGAAAGCT CCCCCACCTC CAAAGCGGGC TCCGACCACG GCCCTCACCC 
     1501 TGCGTTCCAA GTCCATGACA GCGGAGTTGG AGGAACTCGT GGACAAAGCC TCAGTCCGGA 
     1561 AGAAGAAGGA TAAACCGGAA GAGATAGTCC CAGCCTCCAA GCCCTCCAGG ACTGCAGAGA 
     1621 ACGTGGCCAT CGAATCCAGG GTGGCGACCA TCAAGCAGCG GCCCACAAGC CGGTGCTTCC 
     1681 CAGCTGCCTC TGATGTGAAT TCCGTGTACG AGCGCCAAGG GATTGCTGTA ATGACGCCCA 
     1741 CGGTCCCTGG GAGCCCGAAA GGCCCATTTC TGGGCCTCCC TCGAGGTACG ATGCGAAGGC 
     1801 AGAAATCGAT AGACAGCAGA ATCTTTCTAT CAGGGATAAC AGAGGAAGAG CGGCAGTTTC 
     1861 TGGCTCCCCC AATGCTGAAG TTCACCCGAA GCTTGTCCAT GCCAGACACT TCTGAGGACA 
     1921 TCCCCCCTCC GCCACAGTCT GTGCCCCCCT CTCCCCCTCC TCCTTCCCCC ACCACATACA 
     1981 ACTGTCCCAG GTCCCCGACT CCAAGAGTCT ATGGGACAAT TAAGCCCGCG TTCAATCAGA 
     2041 ACCCCGTCGT CGCCAAGGTG CCCCCAGCCA CCAGGTCTGA CACTGTGGCC ACCATGATGC 
     2101 GGGAAAAGGG GATGTTCTAC AGGAGAGAGC TGGACAGATT TTCCCTGGAC TCAGAAGACG 
     2161 TCTACAGCCG CAGCCCCGCC CCACAGGCCG CCTTCCGCAC CAAGCGGGGA CAGATGCCTG 
     2221 AGAACCCGTA CTCAGAGGTG GGAAAGATAG CCAGCAAGGC CGTCTATGTC CCTGCCAAGC 
     2281 CAGCCAGGCG GAAGGGCGTG CTGGTAAAGC AGTCCAACGT GGAGGATAGC CCCGAGAAGA 
     2341 CGTGCTCCAT ACCCATCCCA ACCATCATCG TCAAGGAACC CTCCACCAGC AGCAGCGGCA 
     2401 AGAGCAGCCA GGGGAGCAGC ATGGAGATTG ACCCCCAGGC CACCGAGCCC GGCCAGCTGC 
     2461 GGCCAGATGA CAGCCTCACC GTCAGCAGCC CCTTTGCTGC GGCCATCGCT GGGGCTGTGC 
     2521 GTGACCGGGA GAAGCGTCTG GAAGCCAGGA GGAATTCCCC AGCCTTCCTC TCCACCGACC 
     2581 TGGGAGATGA GGACGTGGGT CTGGGGCCGC CTGCTCCCCG GATGCAGGCC TCCAAGTTCC 
     2641 CAGAGGAGGG TGGGTTTGGT GACGAGGATG AGACGGAACA ACCGCTGTTG CCTACCCCCG 
     2701 GGGCAGCACC CAGGGAGCTG GAGAATCACT TCCTAGGTGG TGGTGAGGCT GGTGCTCAGG 
     2761 GGGAGGCTGG GGGACCCCTG AGTTCCACAT CCAAAGCCAA GGGGCCTGAG AGTGGCCCAG 
     2821 CAGCCCCCCT CAAGAGCAGC AGCCCAGCCG GCCCTGAGAA TTACGTGCAC CCACTCACAG 
     2881 GGCGGCTGCT TGACCCCAGC TCCCCACTGG CCCTGGCACT CTCAGCCAGG GACCGAGCCA 
     2941 TGCAGGAGTC CCAGCAGGGG CACAAAGGAG AGGCCCCCAA GGCTGACCTT AACAAGCCTC 
     3001 TCTACATCGA TACCAAAATG CGGCCCAGCG TGGAGTCCGG CTTTCCTCCA GTCACCAGAC 
     3061 AGAACACCAG GGGTCCCCTG CGACGGCAAG AGACAGAGAA CAAGTACGAG ACAGACCTGG 
     3121 GCAAGGACCG GAGGGCGGAC GACAAGAAGA ACATGCTGAT CAACATCGTG GACACTGCCC 
     3181 AGCAGAAGTC AGCTGGCCTA CTGATGGTGC ACACAGTGGA CGTCCCCATG GCCGGGCCAC 
     3241 CCCTGGAGGA AGAGGAGGAC AGAGAGGATG GGGATACAAA GCCAGACCAC TCACCCTCCA 
     3301 CAGTGCCAGA AGGCGTTCCC AAAACCGAAG GTGCTTTACA GATCTCCGCT GCCCCGGAGC 
     3361 CCGCCGTCGC GCCCGGCAGG ACCATCGTGG CGGCGGGCTC CGTGGAAGAG GCGGTGATTC 
     3421 TGCCATTCCG CATCCCCCCT CCCCCTCTGG CATCCGTGGA CTTGGATGAG GACTTTCTTT 
     3481 TCACAGAACC GTTGCCTCCC CCCCTGGAAT TCGCCAATAG TTTTGATATC CCCGATGACC 
     3541 GGGCAGCTTC AGTTCCCGCT CTGGCTGACC TGGTCAAGCA GAAGAAAAAC GACACCCCTC 
     3601 AGCCCCCTAC GTTGAACTCC AGCCAACCAG CCAACTCCAC AGACAGTAAG AAGCCAGCCG 
     3661 GTATCTCGAA CTGTCTGCCC TCCTCGTTCC TGCCACCCCC CGAAAGTTTC GATGCAGTCA 
     3721 CCGACTCGGG GATTGAGGAG GTGGACAGCC GGAGTAGCAG CGACCACCAC CTGGAGACTA 
     3781 CCAGCACCAT CTCCACGGTG TCCAGCATCT CCACGCTGTC CTCAGAGGGC GGCGAGAGCA 
     3841 TGGACACCTG CACAGTCTAT GCAGACGGGC AAGCCTTTGT GGTTGACAAG CCCCCAGTAC 
     3901 CTCCAAAGCC AAAAATGAAG CCCATCGTTC ACAAGAGCAA CGCACTTTAC CAAGACACGC 
     3961 TCCCAGAAGA GGACACGGAT GGCTTTGTGA TCCCCCCACC TGCACCCCCG CCCCCGCCGG 
     4021 GCAGCGCCCA GGCCGGTGTG GCGAAGGTCA TCCAGCCAAG GACCTCCAAG TTGTGGGGTG 
     4081 ACGTTCCAGA GGTCAAAAGC CCAATTCTCT CAGGCCCAAA GGCAAATGTC ATTAGTGAGC 
     4141 TAAACTCCAT TCTGCAGCAG ATGAACAGGG GGAAATCGGT CAAGCCCGGG GAAGGGCTGG 
     4201 AGCTGCCGGT GGGAGCCAAG TCGGCCAACC TCGCTCCAAG AAGCCCGGAG GTCATGAGCA 
     4261 CCGTCTCAGG TACACGGAGC ACGACGGTCA CCTTCACTGT CCGCCCTGGC ACCTCCCAGC 
     4321 CCATCACCCT GCAGAGCCGG CCCCCCGACT ATGAAAGCAG AACCTCAGGA CCTAGACGCG 
     4381 CCCCAAGCCC TGTGGTTTCA CCAACGGAAT TGAGCAAAGA GATCCTGCCC ACCCCTCCCC 
     4441 CTCCGTCCGC CACTGCAGCC TCTCCCTCCC CCACACTCTC AGATGTCTTT AGCCTTCCGA 
     4501 GCCAGTCCCC TGCAGGGGAC CTCTTTGGCT TGAACCCAGC AGGACGGAGC AGGTCACCAT 
     4561 CTCCTTCAAT ATTGCAACAG CCAATCTCAA ATAAGCCTTT TACAACTAAG CCTGTCCACC 
     4621 TGTGGACGAA ACCAGATGTG GCAGACTGGC TGGAAAGTCT GAACTTGGGT GAACACAAGG 
     4681 AGACGTTCAT GGACAATGAG ATTGACGGCA GCCACCTGCC AAACCTTCAG AAGGAAGACT 
     4741 TGATAGATCT TGGGGTGACT CGAGTTGGGC ATAGGATGAA CATAGAAAGG GCTTTGAAAC 
     4801 AGCTGCTGGA CAGAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4861 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4921 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4981 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5041 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5101 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5161 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5221 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5281 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5341 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5401 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5461 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5521 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5581 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5641 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5701 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5761 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5821 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5881 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5941 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6001 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6061 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6121 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6181 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6241 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6301 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6361 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6421 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6481 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6541 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6601 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6661 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6721 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6781 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6841 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6901 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6961 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7021 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7081 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7141 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7201 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7261 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7321 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7381 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7441 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7501 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7561 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7621 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7681 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7741 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7801 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7861 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8101 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8161 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8221 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8281 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8341 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8401 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8461 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8521 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8581 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8641 ATTTAACAAA AATTTAACGC GAATTTT
//