LOCUS       MR218764                7611 bp    DNA     circular  SYN 
DEFINITION  Mars (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 1
ACCESSION   NM_001171582
VERSION     NM_001171582.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3768..3859 
                     /label="Myc-DDK"  
     misc_feature    3889..4474 
                     /label="PolyA signal"  
     misc_feature    3946..3965 
                     /label="Restriction Enzyme"  
     misc_feature    4750..5422 
                     /label="ColE1"  
     misc_feature    5852..6646 
                     /label="Kan/Neo"  
     misc_feature    6681..6983 
                     /label="SV40 ori"  
     misc_feature    7069..7546 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3750..3756 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3750 
                     /label="Mars"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACTGTTC GTGAGCGAGG GTTCCCCGGG GAGCCTGCCC GTGCTGGCTG 
     1081 CGGCCGCGAG GGCCCGGGGT CGGGCGGAGC TGCTCATCAG CACCGTAGGC CCCGAAGAGT 
     1141 GTGTGGTACC ATTCCTTACC CGGCCTAAGG TCCCTGTCTT GCAGCTGGAT AGTGGCAACT 
     1201 ACCTCTTCTC TGCTAGTGCA ATCTGCCGAT ATTTTTTTCT GTTATGTGGC TGGGAACAAG 
     1261 ATGATCTCAC CAACCAGTGG CTGGAATGGG AGGCAACAGA ACTGCAGCCA GTTCTGTCTG 
     1321 CTGCCCTACA CTGTCTAGTG GTTCAAGGCA AGAAAGGGGA AGATATACTT GGCCCACTTC 
     1381 GGAGAGTCCT GACTCACATT GATCACAGCT TGAGTCGTCA AAACTGTCCT TTCCTGGCTG 
     1441 GGGACACAGA ATCTCTAGCT GACATTGTTT TGTGGGGAGC ACTGTATCCT TTACTGCAAG 
     1501 ACCCAGCTTA CCTCCCTGAG GAGTTGGGTG CCCTGCAAAG TTGGTTCCAG ACACTGAGTA 
     1561 CCCAGGAACC GTGTCAGCGA GCTGCAGAGA CGGTGCTAAA ACAGCAGGGT GTCCTGGCAC 
     1621 TTCGTCTGTA CCTCCAGAAA CAGCCACAGC CTCAGCCCCC GCCTCCTGAG GGGAGAACTG 
     1681 TCAGCAACGA GCTGGAGGAA GAGGAACTGG CTACCTTGTC TGAGGAGGAC ATCGTTACAG 
     1741 CTGTTGCCGC GTGGGAGAAG GGTCTGGAAA GCCTGCCTCC GCTAAAGCTC CAGCAGCATC 
     1801 CAGTGTTGCC TGTGCCTGGA GAGAGGAATG TTCTCATCAC CAGTGCCCTC CCCTATGTCA 
     1861 ACAATGTCCC CCACCTTGGA AACATCATTG GCTGTGTGCT CAGTGCTGAT GTCTTTGCAA 
     1921 GGTATTGTCG CCTTCGCCAG TGGAATACCC TCTATCTGTG TGGTACAGAT GAGTATGGTA 
     1981 CTGCGACAGA GACCAAGGCC ATGGAGGAGG GCCTAACCCC ACGGGAAATC TGTGACAAGT 
     2041 ACCATGCCAT CCATGCTGAC ATCTACCGCT GGTTCGGCAT ATCGTTCGAT ACTTTCGGGC 
     2101 GCACTACCAC TCCTCAGCAG ACCAAAATCA CCCAGGACAT CTTCCAGAGG TTGCTGACCC 
     2161 GGGGGTTTGT GCTGCGAGAT ACTGTGGAGC AGCTTCGGTG TGAGCGGTGT GCACGTTTCC 
     2221 TGGCTGACCG CTTTGTGGAG GGTGTGTGTC CCTTCTGTGG CTATGAAGAG GCCCGAGGTG 
     2281 ACCAGTGTGA CAGGTGTGGC AAGCTCATCA ATGCCATTGA GCTCAAGAAA CCACAGTGCA 
     2341 AAATCTGCCG CTCCTGCCCT GTGGTGAGGT CCTCACAGCA CCTGTTTCTA GACTTGCCTA 
     2401 AGTTGGAAAA GCGTCTGGAG GACTGGTTGG GGAAGACAGT GCCTGGCAGT GACTGGACAC 
     2461 CCAATGCCAG GTTCATTATA CGTTCCTGGC TTCGAGATGG CCTCAAGCCA CGATGCATCA 
     2521 CCAGAGACCT CAAATGGGGA ACGCCTGTGC CCTTGGAAGG TTTTGAGGAC AAGGTATTTT 
     2581 ACGTCTGGTT TGATGCTACT ATTGGCTACG TGTCCATCAC AGCCAACTAC ACAGACCAAT 
     2641 GGGAGAAATG GTGGAAGAAC CCAGAACAAG TGGACCTTTA CCAGTTCATG GCCAAAGACA 
     2701 ATGTTCCCTT CCATGGCTTG GTCTTTCCGT GTTCAGTCCT AGGAGCTGAG GACAACTACA 
     2761 CCCTGGTCAA GCACATCATT GCTACAGAGT ACCTGAACTA TGAGGATGGG AAATTCTCTA 
     2821 AGAGCCGGGG CATAGGAGTG TTTGGAGACA TGGCCAAGGA TACAGGAATC CCTGCTGACA 
     2881 TCTGGCGATT CTATCTGCTA TACATTCGGC CTGAGGGCCA GGACAGTGCC TTCTCCTGGA 
     2941 CAGACTTGTT GATTAAAAAC AATTCTGAGC TGCTCAACAA CCTGGGCAAC TTCATCAACA 
     3001 GAGCTGGCAT GTTTGTTTCT AAGTTTTTTG GCGGTTGTGT GCCTGAGATG GCGCTAACCC 
     3061 CTGATGACAG ACGCCTGGTG GCCCATGTCT CTTGGGAACT CCAGCACTAT CACCAGCTGT 
     3121 TGGAAAAGGT TCGGATCCGG GATGCCTTGC GCAGTATCCT CACCATATCT CGCCATGGCA 
     3181 ACCAATACAT TCAAGTGAAT GAGCCCTGGA AACGGATTAA AGGTGGTGAG ATGGACAGGC 
     3241 AGCGGGCAGG CACAGTGACA GGCATGGCAG TGAACATGGC TGCCTTGCTG TCTGTCATGC 
     3301 TGCAGCCATA CATGCCCACA GTCAGCTCTA CCATCCAGAC CCAGCTGCAG CTCCCACCTG 
     3361 CAGCCTGCCG CATCCTTGCC ACAAGCTTCA TTTGTACCTT GCCAGCAGGC CACCGAATTG 
     3421 GCACAGTCAG TCCTTTGTTC CAAAAACTGG AAAATGACCA GATTGAAAAT TTGAGGCAGC 
     3481 GCTTTGGAGG GGGTCAGCTA GAAGAGTCCT TGGAGTTAAA GGCTAAAGGC TCCCCCAAGC 
     3541 CAGCAGCTGT GGAGGCAGTT ACAGCAGCAG GCTCGCAGCA CATACAAACG CTGACGGATG 
     3601 AGGTGACCAA GCAGGGCAAC GTCGTCCGGG AACTGAAAGC ACAGAAGGCA GACAAGAACC 
     3661 AGGTTGCTGC AGAGGTGGCT AAACTCTTGG ATCTAAAGAA ACAGTTGGCT TTGGCTGAGG 
     3721 GGAAACCCAT TGAAACTCCT AAAGGCAAGA AGAAAAAGAC GCGTACGCGG CCGCTCGAGC 
     3781 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3841 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3901 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3961 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4021 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4081 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4141 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4201 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4261 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4321 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4381 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4441 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4501 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4561 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4621 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4681 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4741 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4801 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4861 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4921 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4981 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5041 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5101 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5161 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5221 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5281 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5341 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5401 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5461 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5521 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5581 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5641 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5701 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5761 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5821 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5881 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5941 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6001 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6061 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6121 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6181 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6241 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6301 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6361 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6421 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6481 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6541 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6601 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6661 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6721 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6781 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6841 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6901 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6961 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7021 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7081 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7141 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7201 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7261 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7321 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7381 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7441 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7501 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7561 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//