LOCUS       MR218699                8796 bp    DNA     circular  SYN 
DEFINITION  Mast3 (Myc-DDK-tagged) - Mouse microtubule associated serine/threonine kinase 3 (Mast3)
ACCESSION   NM_199308
VERSION     NM_199308.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4953..5044 
                     /label="Myc-DDK"  
     misc_feature    5074..5659 
                     /label="PolyA signal"  
     misc_feature    5131..5150 
                     /label="Restriction Enzyme"  
     misc_feature    5935..6607 
                     /label="ColE1"  
     misc_feature    7037..7831 
                     /label="Kan/Neo"  
     misc_feature    7866..8168 
                     /label="SV40 ori"  
     misc_feature    8254..8731 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4935..4941 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4935 
                     /label="Mast3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGACCCC AGCTACTGGA CCGCGGTCGC GGCCCCGGGG CACCGCAGCC 
     1081 GCCTGGCCAA GGGTGCGCTG CTACAGCGTT CCAAAAGCTG CCGCAGTGGC AACCGTAAGA 
     1141 GCTTGGTCGT GGGGACGCCC TCACCCACTC TCTCCAGACC TCTGTCACCA CTCTCTGTCC 
     1201 CAACTGCAGG TAACAGCCCC CTGGACAGTC CTCGGAACTT CTCTGCTGCA GCAGCCATCA 
     1261 GTTTCCCCTT TGCCCGGAGG GCTGACGGCA GAAGATGGTC ACTTGCTTCC CTCCCGTCCT 
     1321 CGGGCTATGG GACCAACACT CCCAGCTCCA CTGTATCGTC AAGCTCGTCC TCCCGGGAGC 
     1381 GTCTACACCA GCTCCCCTTC CAGCCCACAG CAGACGAGCT GCGCTTCCTG TCCAAGCACT 
     1441 TCCGCAGCTC AGAGAGTGTG GTGGACGAGG ACGGAGGCCG TTCTCCACGC CTGCGCCCAC 
     1501 GCTCACGCAG CCTCAGCCCT GGACGCACAT CAGGAACCTT CGACAACGAA ATAGTGATGA 
     1561 TGAACCACGT GTACCGGGAG CGTTTCCCCA AGGCCACGGC GCAGATGGAG GGCCGGCTGC 
     1621 AGGACTTCCT GGCAGCATTT GCTCCTGGCG ACCGCCTGGC ACTGGCAGAC GGTGTCTTGG 
     1681 GCTTTATCCA CCACCAGATC GTGGAACTGG CACGGGACTG CCTGGCCAAG TCGGGCGAGG 
     1741 CTCTGGTCAC TTCCCGCTAT TTCCTGGAGA TGCAGGACAA GCTGGAGAGG CTGTTGCAGG 
     1801 ATGCTCACGA GCGGTCGGAC AGCGCCGAGG TGGGCTTCAT AGTGCAGCTG GTCCGAAAGC 
     1861 TGCTCATCAT CATCTCCAGG CCAGCGCGGC TGCTCGAGTG CTTGGAGTTT GACCCGGAAG 
     1921 AATTCTACCA TCTGCTGGAG GCTGCAGAAG GCCAGGCCCG CGAGGACCAG GGCGTGAAGA 
     1981 CTGATCTGCC CCGCTACATC ATCCGCCAGC TGGGCCTTGC CAAGGACCCG CTGGAAGAGA 
     2041 TCCAGCCCCT GAATGACCTT GATGAAAGCC AGCCCCCTGC TCCTGGATCC CCTGAGAGCC 
     2101 GAGGCCTGGG TGGTCCGTCT CGGAGGAAGC CATGTGAGAG CGACTTTGAG ACCATCAAGC 
     2161 TCATTAGCAA TGGAGCCTAT GGGGCCGTCT ACCTGGTGCG CCACAGGGAT ACGCGGCAGC 
     2221 GCTTCGCCAT CAAGAAAATC AACAAGCAGA ATTTAATTCT GCGCAACCAG ATCCAACAGG 
     2281 TGTTTGTGGA GCGAGACATC CTCACCTTCG CCGAGAACCC CTTTGTGGTT GGCATGTTCT 
     2341 GCTCCTTTGA GACCCGCCGC CACCTCTGTA TGGTCATGGA ATATGTGGAA GGGGGTGACT 
     2401 GCGCTACGCT CCTGAAAAAC ATGGGCCCAC TGCCCGTGGA CATGGCACGC ATGTACTTCG 
     2461 CGGAGACGGT GCTGGCACTG GAATATCTGC ACAACTATGG CATTGTGCAC CGAGATCTCA 
     2521 AACCTGACAA CCTGCTCATC ACCTCCCTCG GCCATATCAA GCTGACGGAC TTCGGCCTGT 
     2581 CCAAGATCGG CCTCATGAGC ATGGCCACCA ACCTGTATGA AGGTCACATC GAGAAGGATG 
     2641 CTCGAGAGTT TGTGGACAAG CAGGTGTGTG GGACACCCGA GTACATCGCA CCAGAGGTGA 
     2701 TCTTCCGCCA GGGCTACGGG AAGCCAGTAG ACTGGTGGGC CATGGGCGTC ATCCTGTATG 
     2761 AGTTCCTGGT GGGCTGTGTG CCATTCTTCG GGGACACGCC TGAGGAGCTG TTCGGACAGG 
     2821 TTGTCAGTGA TGAGATCATG TGGCCAGAGG GAGATGAGGC CCTTCCCCTT GACGCTCAGG 
     2881 ACCTGATTAC TAGGCTTCTG CGTCAGAGCC CAATGGACAG GCTGGGGACT GGTGGCACCC 
     2941 ATGAGGTGAA ACAGCACCCC TTCTTCCTGG CCCTGGACTG GGCAGGGCTC CTGAGACACA 
     3001 AGGCAGAGTT TGTCCCACAG CTTGAAGCTG AGGATGACAC CAGCTACTTT GACACTCGCT 
     3061 CTGAGCGGTA CCGCCACTTG GGCTCCGAGG ACGACGAGAC GAATGATGAG GAGTCCTCCA 
     3121 CGGAGATCCC CCAGTTCTCC TCCTGTTCCC ACCGCTTCAG CAAGGTTTAC AGCAGCTCTG 
     3181 AGTTCCTGGC CGTCCAGCCC ACCCCAACCT TTGCTGAAAG GAGCTTCAGT GAGGACCGAG 
     3241 AAGACGGCTG GGGGCAAAGC CTCGGGGACC GTGGCCGCCG GCTGAGCGCT GACCTCCGGC 
     3301 TAAGGTCCTG GACACCTGCC TCTTCCTGTC AGCCATCTTC CTGTCAGACT GACCGGGGTC 
     3361 CTAGCCCGTC TCTCCTAAGC ACTATCAGTT TGGATGTGAT GCCTAAATTT GCCTTCTCCT 
     3421 CTGAAGATGA GGGAGCAAGT TCAGGACCTG CTGATCCCCA AAAGCCTGTC TTCATTCTGG 
     3481 GGGAGCCTGA TCCCCCACCC CCGACCACCC CAGTGACACC CAAGCCCTGC AACCTTTCTG 
     3541 CTGACACAGC TGTCCTCAGC CATGCCCGCC TTCGAAGTAA CAGTACAGGA GCCCGGCACT 
     3601 CCACTCCTCG GCCCCTGGAT GCTGGCAGAG GCCGCCGCCT AGGAGGCTCA AGAGATCCTG 
     3661 GCCCTGAGAA GCCCCGGGCC TCCCCTGGTG GCAGTGGGGG TCGAGTGCCC AAGTCAGCCT 
     3721 CTGTGTCTGC CCTGTCCCTC ATCATCACGG CAGACGACGG CAGCGGGGGC CCTCTCATGA 
     3781 GCCCCTTGTC GCCCCGCTCG CTGTCCTCCA ATCCCTCCTC CCGAGACTCC TCACCCAGCC 
     3841 GGGACCCATC ACCAGTGTGT GGTAGCCTGC GGCCTCCCAT CGTCATCCAC AGCTCAGGCA 
     3901 AAAAGTATGG CTTCAGCCTG CGCGCCATTC GTGTCTACAT GGGTGATAGT GACGTGTACA 
     3961 CGGTGCACCA TGTGGTCTGG AGCGTGGAGG AGGGGAGCCC TGCACAGGAG GCAGGACTGC 
     4021 GTGCTGGGGA CCTCATCACA CACATCAATG GGGAGTCAGT CCTGGGCCTG GTACACATGG 
     4081 ACGTGGTGGA GCTGCTGCTG AAGAGTGGCA ACAAGATCTC CCTGCGCACC ACGGCCCTGG 
     4141 AGAACACCTC CATCAAGGTG GGACCCGCGC GGAAGAACGT GGCCAAGGGC CGCATGGCCC 
     4201 GTAGGAGTAA GCGCAGCCGC CGGCGTGAGA CCCAGGACCG GCGGAAGTCT CTGTTTAAGA 
     4261 GGATCTCAAA GCCATCTTCT GTGCTCCACA CCAGCCGTAG CTTTTCCTCA GGCCTCCAGC 
     4321 ACTCTCTGTC CTCCAGCGAG AGCCTCCCCG GATCACCCAC TCACAGCCTG TCTCCCAGCC 
     4381 CCACCACGCC CTGCCGCAGC CCTGCCCCCG ACGCCCCCAC AGACACAGCA TCCCCACCCA 
     4441 ATGTGTCCCC AAGCTCCAGC AGCCCCGCTT CTCCAGCCAC TGGTCACACC CGTCCCAGCT 
     4501 CCCTGCATGG CCTGGCAGCC AAGCTCGGAC CACCTCGTCA CAAGAGCGGT CGCCGCAAGT 
     4561 CCACAAGCAG TATCCCTCCA TCTCCGCTGG CCTGCCCGCC CGTGCCCACG CCCCCGCCAC 
     4621 GCTCACCATC CCCCCTTCCG GGGCACATCC CCATACCCGC CCGGTCTCCT AGGCTGCGTA 
     4681 GGGGCCAGTC AGCCGACAAG CTGGGCCTAG GCACCAGTGA GAGGCTGGAT GGGGATGGAG 
     4741 GCCGGCGCGC CCGGGGGGCC GAGGCTGAGC TGGTCGTCAT GAGGAGGCTG CATCTATCAG 
     4801 AGCGTCGGGA TTCCTTCAAG AAGCAGGAGG CAGTGCAGGA GGTGAGCTTC GATGAGGAGC 
     4861 CTGGGCCGCC TCGCGGGGTG CCGAAGATCG CTGTGCAGGG TGCAGAGGCC ACACCTGGAA 
     4921 CCCCAGGACA TGCCAGGAAA GACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4981 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5041 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5101 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5161 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5221 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5281 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5341 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5401 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5461 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5521 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5581 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5641 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5701 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5761 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5821 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5881 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5941 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6001 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6061 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6121 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6181 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6241 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6301 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6361 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6421 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6481 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6541 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6601 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6661 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6721 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6781 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6841 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6901 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6961 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7021 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7081 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7141 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7201 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7261 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7321 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7381 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7441 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7501 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7561 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7621 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7681 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7741 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7801 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7861 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7921 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7981 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8041 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8161 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8221 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8281 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8341 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8401 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8461 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8521 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8581 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8641 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8701 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8761 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//