LOCUS       MR218696                9390 bp    DNA     circular  SYN 
DEFINITION  Arhgap5 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 5 (Arhgap5)
ACCESSION   NM_009706
VERSION     NM_009706.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5547..5638 
                     /label="Myc-DDK"  
     misc_feature    5668..6253 
                     /label="PolyA signal"  
     misc_feature    5725..5744 
                     /label="Restriction Enzyme"  
     misc_feature    6529..7201 
                     /label="ColE1"  
     misc_feature    7631..8425 
                     /label="Kan/Neo"  
     misc_feature    8460..8762 
                     /label="SV40 ori"  
     misc_feature    8848..9325 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5529..5535 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5529 
                     /label="Arhgap5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCAAAA AACAAAGAGC CTCGACCCCC ATCTTATACT GTCAGTGTAG 
     1081 TTGGACTCTC TGGGACTGAA AAAGACAAAG GAAACTGTGG AGTTGGAAAA TCTTGTTTGT 
     1141 GCAATAGATT TGTACGTTCA AAAGCAGATG AATATTATCC AGAGCATACT TCTGTGCTTA 
     1201 GCACCATTGA CTTTGGAGGC CGAGTAGTAA ACAATGATCA CTTTTTATAC TGGGGTGACA 
     1261 TAACACAAAA TGGTGAAGAT GGTGTAGAAT GCAAAATTCA TGTCATTGAA CAAACAGAGT 
     1321 TCATTGATGA CCAGACTTTC TTGCCTCATC GAAGTACAAA TTTGCAACCA TATATAAAAC 
     1381 GTGCAGCTGC CTCAAAATTG CAGTCAGCAG AAAAACTAAT GTACATTTGT ACCGATCAGC 
     1441 TAGGCTTGGA GCAAGACTTT GAACAGAAGC AAATGCCTGA AGGGAAACTC AATGTAGATG 
     1501 GATTTTTATT GTGTATTGAT GTGAGTCAAG GATGTAATAG GAAGTTTGAT GATCAACTTA 
     1561 AATTTGTGAA TAACCTTTTT GTCCAGCTAT CAAAATCCAA AAAGCCTGTG ATAATAGCAG 
     1621 CAACTAAATG TGATGAATGT GTGGATCATT ACCTTAGAGA AGTTCAAGCC TTTGCCTCAA 
     1681 ACAAAAAGAA TCTTCTAGTA GTGGAAACAT CAGCACGATT TAATGTCAAC ATTGAGACAT 
     1741 GTTTCACTGC TTTGGTACAA ATGTTGGATA AAACTCGTGG CAAACCTAAA ATTATTCCCT 
     1801 ATCTGGATGC TTATAAAACA CAGAGACAAC TTGTTGTCAC AGCAACAGAC AAGTTTGAAA 
     1861 AACTTGTACA GACTGTGAGA GATTATCATG CAACTTGGAA AACTGTTAGT AATAAATTAA 
     1921 AAAATCATCC TGATTATGAA GAATATATCA ACTTAGAGGG AACAAGAAAG GCCAGAAATA 
     1981 CATTCTCAAA GCATATAGAG CAACTCAAAC AGGAACATAT AAGAAAAAGG AGAGAAGAAT 
     2041 ATATAAGTAC CTTACCAAGG GCTTTTAATA CTCTCTTGCC AGATCTAGAA GAGATTGAAC 
     2101 ATTTGAATTG GTTGGAAGCT TTGAAGTTAA TGGAAAAGAG AGCAGATTTC CAGTTATGTT 
     2161 TTGTGGTGCT AGAAAAAACA CCTTGGGATG AAACTGACCA TATAGACAAA ATTAATGATA 
     2221 GACGGATCCC ATTCGACCTT CTGAGCACTT TAGAAGCAGA AAAAGTCTAT CAAAACCATG 
     2281 TACAACATCT GATATCAGAG AAAAGAAGAA TAGAAATGAA GGAGAAATTC AAGAAGACTT 
     2341 TAGAAAAAAT TCAGTTCATT TCACCTGGGC AGCCATGGGA GGAAGTTATG TGTTTTGTCA 
     2401 TGGAGGATGA AGCATTCAAA TACATCACTG AGGCTGATAG CAAAGAAGTA TATGGTAGGC 
     2461 ATCAGCGAGA GATAGTAGAA AAAGCCAAAG AAGAGTTTCA GGAAATGCTT TTTGAGCATT 
     2521 CTGAACTTTT TTATGATTTA GATCTTAATG CAACACCAAG TTCTGATAAA ATGAGTGAAA 
     2581 TTCATACCGT TCTAAGTGAA GAACCTAGAT ATAAAGCTTT ACAGAAACTT GCACCTGATA 
     2641 GAGAATCTCT TCTACTTAAG CATATAGGAT TTGTTTATCA TCCCACTAAA GAAACATGCC 
     2701 TCAGTGGCCA ATATTGTACA GACATTAAAG TGGAAAATTT ACTTGCCACT AGTCTATTAG 
     2761 AGATGGATCA TAACCGCGTA CGCTTGTATC ATGATAGTAC CAATATAGAC AAAGTTAATC 
     2821 TTTTCATTTT AGGGAAAGAT GGCCTTGCCC AGGAACTAGC AAATGAGATA AGGACTCAAT 
     2881 CCACTGATGA TGAGTATGCC TTAGATGGAA AAATTTATGA ACTTGATCTT CGGCCTGTTG 
     2941 ATGCCAAATC GCCTTACATT TTGAGTCAGC TATGGACTGC AGCCTTTAAA CCACATGGGT 
     3001 GCTTCTGTGT ATTCAATTCC ATCGAGTCAC TGAGTTTTAT TGGAGAATTT ATTGGAAAAA 
     3061 TAAGAACCGA AGCATCTCAG ATCAGAAAAG ATAAATATAT GACTAATCTT CCATTTACAT 
     3121 TAATTCTTGC TAATCAGAGG GATTCCATTA GTAAAAATCT ACCAATTCTC AGGCACCAGG 
     3181 GTCAGCAGTT GGCCAACAAA TTGCAGTGTC CTTTTGTAGA CGTACCTACT GGTACATATC 
     3241 CTCGTAAATT TAATGAATCA CAAATAAAGC AAGCTCTAAG AGGAGTATTG GAATCAGTTA 
     3301 AACACAATTT AGATGTGGTG AGCCCAGTTC CTATCAATAA GGATGTGTCA GAAGCTGACT 
     3361 TGAGAATTGT CATGTGTGCC ATGTGTGGCG ATCCATTTAG TGTGGATCTC ATTCTTTCAC 
     3421 CTTTCCTTGA CTCTCATTCT TGCAGTGCTG CTCAAGCTGG ACAGAATAAT TCTTTAATGC 
     3481 TTGACAAAAT TATTGGTGAA AAAAGGAGGC GAATACAGAT CACAATATTG TCATACCACT 
     3541 CCTCAATTGG AGTAAGAAAA GATGAACTGG TTCATGGGTA TATATTAGTT TATTCTGCAA 
     3601 AAAGGAAAGC ATCAATGGGA ATGCTTCGTG CATTTCTATC AGAAGTTCAA GATACTATTC 
     3661 CTGTACAACT GGTGGCAGTT ACTGACAGTC AAGCTGATTT CTTTGAAAAT GAGGCTATCA 
     3721 AGGAGTTAAT GACTGAAGGA GAACACATTG CAACTGAGAT AACCGCTAAA TTTACAGCAT 
     3781 TATATTCTTT ATCTCAGTAT CATAGGCAAA CTGAGGTTTT CACTTTGTTT TTCAGTGATG 
     3841 TTCTAGAGAA AAAAAATATG ATAGAAAATT CCTATTTGTC TGATAATACA AGGGAATCCA 
     3901 CTCATCAGAG TGAAGATGTT TTTCTACCGT CTCCAAGAGA CTGTTTTCCC TATAACAACT 
     3961 ACCCTGATTC AGATGATGAC ACAGAAGCAC CACCTCCATA TAGTCCAATT GGAGATGATG 
     4021 TACAGTTGCT TCCAACACCT AGTGACCGTT CCAGATACAG GTTAGATTTG GAAGGAAATG 
     4081 AGTATCCTGT TCATAGCACT CCAAATTGTC ACGATCATGA ACGTAACCAT AAAGTGCCTC 
     4141 CACCTATTAA ACCTAAACCA GTTGTACCTA AAACAAATGT GAAAAAACTG GATCCAAACC 
     4201 TTTTAAAAAC AATTGAAGCT GGTATTGGTA AAAATCCAAG AAAACAGACT TCCCGGGTGC 
     4261 CTTTGGCACA TCCTGAAGAT ATGGATTCTT CAGATAACTA TGTGGAACCC CTTGACACAA 
     4321 TTTTCAAGCA GAAGGGCTAT TCTGATGAGA TTTATGTTGT CCCAGATGAT AGTCAGAATC 
     4381 GAATTATTAA AATTCGAAAC TCATTTGTAA ATAACACTCA AGGAGATGAA GAAAATGGGT 
     4441 TTTCTGATAG AACCTCAAAA GGTCATGGAG AGCGTAGGCC TTCAAAATAT AAATATAAAT 
     4501 CTAAAACTTT GTTTAGTAAA GCCAAGTCAT ACTACAGAAG AACACACTCA GATGCAAGCG 
     4561 ATGATGAGGC TTTCACTACT TCCAAAACAA AAAGAAAAGG AAGACATCGT GGAAGTGAAG 
     4621 AAGATCCACT ACTGTCTCCT GTTGAAACTT GGAAAGGTGG TATTGATAAT CCTGCAATCA 
     4681 CATCTGACCA GGAGGTAGAT GATAAGAAGA TAAAGAAGAA AACCCACAAA GTAAAGGAAG 
     4741 ATAAAAAGCA GAAAAAGAAA ACTAAGACCT TCAACCCACC AACACGTAGA AATTGGGAAA 
     4801 GTAATTACTT TGGGATGCCC CTCCAGGATC TGGTTACAGC TGAGAAGCCT ATACCACTAT 
     4861 TTGTTGAAAA ATGTGTGGAA TTTATTGAAG ACACAGGATT ATGTACTGAA GGACTATACC 
     4921 GTGTTAGTGG AAATAAAACT GATCAAGACA ACATTCAAAA GCAGTTTGAT CAAGATCATA 
     4981 ATATCAATCT AGCATCAATG GAAGTGACAG TCAATGCTGT AGCTGGAGCT CTCAAAGCTT 
     5041 TCTTTGCTGA CCTGCCGGAT CCTTTGATTC CATATTCACT CCACCCAGAG CTATTGGAAG 
     5101 CAGCAAAAAT CCCAGATAAA ACAGAGCGCT TTCATGCCTT GAAAGAAATT GTTAAGAAAT 
     5161 TTCATCCTGT AAACTATGAT GTATTCAGAT ATGTGATAAC ACATCTAAAC AGGGTTAGTC 
     5221 AGCAAAATAA AATCAACCTA ATGACAGCAG ACAACTTATC CATCTGTTTT TGGCCAACTT 
     5281 TGATGAGACC TGATTTTGAA AATCGAGAGT TTCTGTCTAC CACTAAAATC CATCAATCTG 
     5341 TCGTTGAAAC ATTTATTCAA CAGTGCCAGT TTTTCTTTTA CAATGGAGAA ATTGTAGAAA 
     5401 CTGCGAACAC TGTGGCTCCT CCACCTACTT CAAATCCAGG ACAATTGGTA GAATCAATGG 
     5461 TACCACTTCA GTTGCCACCA CCATTGCAAC CTCAGCTAAT TCAACCACAA TTGCAGACAG 
     5521 ATCCTCTTGG TATTATAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5581 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5641 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5701 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5761 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5821 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5881 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5941 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     6001 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     6061 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6121 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6181 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6241 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6301 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6361 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6421 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6481 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6541 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6601 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6661 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6721 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6781 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6841 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6901 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6961 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     7021 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     7081 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7141 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7201 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7261 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7321 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7381 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7441 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7501 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7561 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7621 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7681 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7741 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7801 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7861 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7921 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7981 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     8041 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     8101 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8161 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8221 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8281 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8341 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8401 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8461 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8521 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8581 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8641 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8701 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8761 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8821 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8941 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     9001 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     9061 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9121 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9181 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9241 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9301 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9361 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//