LOCUS       MR218690                8637 bp    DNA     circular  SYN 
DEFINITION  Cfh (Myc-DDK-tagged) - Mouse complement component factor h (Cfh)
ACCESSION   NM_009888
VERSION     NM_009888.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4794..4885 
                     /label="Myc-DDK"  
     misc_feature    4915..5500 
                     /label="PolyA signal"  
     misc_feature    4972..4991 
                     /label="Restriction Enzyme"  
     misc_feature    5776..6448 
                     /label="ColE1"  
     misc_feature    6878..7672 
                     /label="Kan/Neo"  
     misc_feature    7707..8009 
                     /label="SV40 ori"  
     misc_feature    8095..8572 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4776..4782 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4776 
                     /label="Cfh"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTACAGCAC AGATTTCTCT TGGAGTCAGT TGGTCCCAGA AAGATCCAAA 
     1081 TTATGAGACT GTCAGCAAGA ATTATTTGGC TTATATTATG GACTGTTTGT GCAGCAGAAG 
     1141 ATTGTAAAGG TCCTCCTCCA AGAGAAAATT CAGAAATTCT CTCAGGCTCG TGGTCAGAAC 
     1201 AACTATATCC AGAAGGCACC CAGGCTACCT ACAAATGCCG CCCTGGATAC CGAACACTTG 
     1261 GCACTATTGT AAAAGTATGC AAGAATGGAA AATGGGTGGC GTCTAACCCA TCCAGGATAT 
     1321 GTCGGAAAAA GCCTTGTGGG CATCCCGGAG ACACACCCTT TGGGTCCTTT AGGCTGGCAG 
     1381 TTGGATCTCA ATTTGAGTTT GGTGCAAAGG TTGTTTATAC CTGTGATGAT GGGTATCAAC 
     1441 TATTAGGTGA AATTGATTAC CGTGAATGTG GTGCAGATGG GTGGATCAAT GATATTCCAC 
     1501 TATGTGAAGT TGTGAAGTGT CTACCTGTGA CAGAACTCGA GAATGGAAGA ATTGTGAGTG 
     1561 GTGCAGCAGA AACAGACCAG GAATACTATT TTGGACAGGT GGTGCGGTTT GAATGCAATT 
     1621 CAGGCTTCAA GATTGAAGGA CATAAGGAAA TTCATTGCTC AGAAAATGGC CTTTGGAGCA 
     1681 ATGAAAAGCC ACGATGTGTG GAAATTCTCT GCACACCACC GCGAGTGGAA AATGGAGATG 
     1741 GTATAAATGT GAAACCAGTT TACAAGGAGA ATGAAAGATA CCACTATAAG TGTAAGCATG 
     1801 GTTATGTGCC CAAAGAAAGA GGGGATGCCG TCTGCACAGG CTCTGGATGG AGTTCTCAGC 
     1861 CTTTCTGTGA AGAAAAGAGA TGCTCACCTC CTTATATTCT AAATGGTATC TACACACCTC 
     1921 ACAGGATTAT ACACAGAAGT GATGATGAAA TCAGATATGA ATGTAATTAT GGCTTCTATC 
     1981 CTGTAACTGG ATCAACTGTT TCAAAGTGTA CACCCACTGG CTGGATCCCT GTTCCAAGAT 
     2041 GTACCTTGAA ACCATGTGAA TTTCCACAAT TCAAATATGG ACGTCTGTAT TATGAAGAGA 
     2101 GCCTGAGACC CAACTTCCCA GTATCTATAG GAAATAAGTA CAGCTATAAG TGTGACAACG 
     2161 GGTTTTCACC ACCTTCTGGG TATTCCTGGG ACTACCTTCG TTGCACAGCA CAAGGGTGGG 
     2221 AGCCTGAAGT CCCATGCGTC AGGAAATGTG TTTTCCATTA TGTGGAGAAT GGAGACTCTG 
     2281 CATACTGGGA AAAAGTATAT GTGCAGGGTC AGTCTTTAAA AGTCCAGTGT TACAATGGCT 
     2341 ATAGTCTTCA AAATGGTCAA GACACAATGA CATGTACAGA GAATGGCTGG TCCCCTCCTC 
     2401 CCAAATGCAT CCGTATCAAG ACATGTTCAG CATCAGATAT ACACATTGAC AATGGATTTC 
     2461 TTTCTGAATC TTCTTCTATA TATGCTCTAA ATAGAGAAAC ATCCTATAGA TGTAAGCAGG 
     2521 GATATGTGAC AAATACTGGA GAAATATCAG GATCAATAAC TTGCCTTCAA AATGGATGGT 
     2581 CACCTCAACC CTCATGCATT AAGTCTTGTG ATATGCCTGT ATTTGAGAAT TCTATAACTA 
     2641 AGAATACTAG GACATGGTTT AAGCTCAATG ACAAATTAGA CTATGAATGT CTCGTTGGAT 
     2701 TTGAAAATGA ATATAAACAT ACCAAAGGCT CTATAACATG TACTTATTAT GGATGGTCTG 
     2761 ATACACCCTC ATGTTATGAA AGAGAATGCA GTGTTCCCAC TCTAGACCGA AAACTAGTCG 
     2821 TTTCCCCCAG AAAAGAAAAA TACAGAGTTG GAGATTTGTT GGAATTCTCC TGCCATTCAG 
     2881 GACACAGAGT TGGGCCAGAT TCAGTGCAAT GCTACCACTT TGGATGGTCT CCTGGTTTCC 
     2941 CTACATGTAA AGGTCAAGTA GCATCATGTG CACCACCTCT TGAAATTCTT AATGGGGAAA 
     3001 TTAATGGAGC AAAAAAAGTT GAATACAGCC ATGGTGAAGT GGTGAAATAT GATTGCAAAC 
     3061 CTAGATTCCT ACTGAAGGGA CCCAATAAAA TCCAGTGTGT TGATGGGAAT TGGACAACCT 
     3121 TGCCTGTATG TATTGAGGAG GAGAGAACAT GTGGAGACAT TCCTGAACTT GAACATGGCT 
     3181 CTGCCAAGTG TTCTGTTCCT CCCTACCACC ATGGAGATTC AGTGGAGTTC ATTTGTGAAG 
     3241 AAAACTTCAC AATGATTGGA CATGGGTCAG TTTCTTGCAT TAGTGGAAAA TGGACCCAGC 
     3301 TTCCTAAATG TGTTGCAACA GACCAACTGG AGAAGTGTAG AGTGCTGAAG TCAACTGGCA 
     3361 TAGAAGCAAT AAAACCAAAA TTGACTGAAT TTACGCATAA CTCCACCATG GATTACAAAT 
     3421 GTAGAGACAA GCAGGAGTAC GAACGCTCAA TCTGTATCAA TGGAAAATGG GATCCTGAAC 
     3481 CAAACTGTAC AAGCAAAACA TCCTGCCCTC CTCCACCGCA GATTCCAAAT ACCCAAGTGA 
     3541 TTGAAACCAC CGTGAAATAC TTGGATGGAG AAAAATTATC TGTTCTTTGC CAAGACAATT 
     3601 ACCTAACTCA GGACTCAGAA GAAATGGTGT GCAAAGATGG AAGGTGGCAG TCATTACCTC 
     3661 GCTGCATTGA AAAAATTCCA TGTTCCCAGC CCCCTACAAT AGAACATGGA TCTATTAATT 
     3721 TACCCAGATC TTCAGAAGAA AGGAGAGATT CCATTGAGTC CAGCAGTCAT GAACATGGAA 
     3781 CTACATTCAG CTATGTCTGT GATGATGGTT TCAGGATACC TGAAGAAAAT AGGATAACCT 
     3841 GCTACATGGG AAAATGGAGC ACTCCACCTC GCTGTGTTGG ACTTCCTTGT GGACCTCCAC 
     3901 CTTCAATTCC TCTTGGTACT GTTTCTCTTG AGCTAGAGAG TTACCAACAT GGGGAAGAGG 
     3961 TTACATACCA TTGTTCTACA GGCTTTGGAA TTGATGGACC AGCATTTATT ATATGCGAAG 
     4021 GAGGAAAGTG GTCTGACCCA CCAAAATGCA TAAAAACGGA TTGTGACGTT TTACCCACAG 
     4081 TTAAAAATGC CATAATAAGA GGAAAGAGCA AAAAATCATA TAGGACAGGA GAACAAGTGA 
     4141 CATTCAGATG TCAATCTCCT TATCAAATGA ATGGCTCAGA CACTGTGACA TGTGTTAATA 
     4201 GTCGGTGGAT TGGACAGCCA GTATGCAAAG ATAATTCCTG TGTGGATCCA CCACATGTGC 
     4261 CAAATGCTAC TATAGTAACA AGGACCAAGA ATAAATATCT ACATGGTGAC AGAGTACGTT 
     4321 ATGAATGTAA TAAACCTTTG GAACTATTTG GGCAAGTGGA AGTGATGTGT GAAAATGGGA 
     4381 TATGGACAGA AAAACCAAAG TGCCGAGACT CAACAGGGAA ATGTGGGCCT CCTCCACCTA 
     4441 TTGACAATGG AGACATCACC TCCTTGTCAT TACCAGTATA TGAACCATTA TCATCAGTTG 
     4501 AATATCAATG CCAGAAGTAT TATCTCCTTA AGGGAAAGAA GACAATAACA TGTAGAAATG 
     4561 GAAAGTGGTC TGAGCCACCA ACATGCTTAC ATGCATGTGT AATACCAGAA AACATTATGG 
     4621 AATCACACAA TATAATTCTC AAATGGAGAC ACACTGAAAA GATTTATTCC CATTCAGGGG 
     4681 AGGATATTGA ATTTGGATGT AAATATGGAT ATTATAAAGC AAGAGATTCA CCGCCATTTC 
     4741 GTACAAAGTG CATTAATGGC ACCATCAATT ATCCCACTTG TGTAACGCGT ACGCGGCCGC 
     4801 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4861 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4921 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4981 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5041 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5101 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5161 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5221 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5281 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5341 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5401 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5461 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5521 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5581 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5641 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5701 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5761 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5821 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5881 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5941 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6001 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6061 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6121 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6181 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6241 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6301 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6361 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6421 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6481 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6541 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6601 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6661 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6721 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6781 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6841 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6901 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6961 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7021 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7081 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7141 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7201 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7261 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7321 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7381 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7441 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7501 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7561 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7621 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7681 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7741 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7801 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7861 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7921 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8041 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8101 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8161 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8221 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8281 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8341 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8401 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8461 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8521 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8581 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//