LOCUS       MR218639                7194 bp    DNA     circular  SYN 
DEFINITION  Adam33 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 33 (Adam33), transcript variant 2
ACCESSION   NM_001163529
VERSION     NM_001163529.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3351..3442 
                     /label="Myc-DDK"  
     misc_feature    3472..4057 
                     /label="PolyA signal"  
     misc_feature    3529..3548 
                     /label="Restriction Enzyme"  
     misc_feature    4333..5005 
                     /label="ColE1"  
     misc_feature    5435..6229 
                     /label="Kan/Neo"  
     misc_feature    6264..6566 
                     /label="SV40 ori"  
     misc_feature    6652..7129 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3333..3339 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3333 
                     /label="Adam33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTCGAGG TGCGGGAGAC CCGGGGGGTC TCCGGTGCTG CTATTGCTGC 
     1081 CGCTGTTGCT GCCCTCGTGT CCGCTGCGGA GCGCTCGGAT GTTTCCAGGA AATGCCCATG 
     1141 GAGAGCTAGT CACTCCCCAC TGGATCCTGG AGGGCAGACT CTGGCTCAAG GTCACCCTGG 
     1201 AGGAGCCGAT CTTGAAGCCT GACTCGGTGC TGGTGGCTTT AGAGGCTGAA GGCCAGGATC 
     1261 TCCTGCTTGA ACTGGAGAAG AAGCACAAGC TTCTGGCCCC AGGATACACA GAAACCCACT 
     1321 ACAGGCCAGA TGGGCATCCG GTAGTGCTGT CCCCCAACCA CACGGATCAT TGCCAATATC 
     1381 ACGGGCGTGT GAGGGGCTTC CGGGAATCCT GGGTGGTTCT CAGCACCTGC TCTGGGATGA 
     1441 GTGGCCTTAT TGTGCTCAGC AGCAAAGTCA GCTATTATCT GCAACCTCGG ACTCCTGGGG 
     1501 ATACCAAAGA CTTCCCAACC CACGAGATCT TCCGGATGGA GCAGTTGTTC ACCTGGAGAG 
     1561 GGGTCCAGAG AGACAAGAAC TCCCAATACA AAGCAGGAAT GGCCAGTCTT CCTCATGTCC 
     1621 CCCAGAGCCG GGTGAGGCGA GAGGCGCGCA GGAGTCCCAG GTACCTGGAA CTGTACATAG 
     1681 TGGCTGACCA CACCCTGTTC TTGCTTCAGC ATCAGAACTT GAACCACACG AGACAGCGCC 
     1741 TCCTGGAGGT TGCCAATTGC GTGGACCAGA TTCTCAGGAC TCTGGATATA CAGTTGGTGT 
     1801 TGACCGGGCT GGAAGTGTGG ACCGAGCAGG ATCTCAGTCG CATCACTCAG GACGCAAACG 
     1861 AAACGCTCTG GGCTTTCCTA CAGTGGCGCC GCGGGGTGTG GGCCAGGAGA CCACACGACT 
     1921 CCACACAACT GCTCACGGGC CGCACCTTCC AGGGTACCAC GGTGGGCCTG GCACCTGTGG 
     1981 AGGGCATATG CCGCGCGGAG AGCTCCGGAG GTGTGAGCAC AGACCACTCG GAACTCCCCA 
     2041 TCGGCACAGC AGCCACCATG GCCCACGAGA TAGGCCACAG CCTGGGCCTC CACCATGATC 
     2101 CCGAGGGCTG CTGCGTGCAG GCCGATGCAG AGCAAGGAGG CTGCGTCATG GAGGCAGCCA 
     2161 CAGGGCACCC TTTCCCGCGC GTCTTCAGCG CCTGCAGCCG CCGCCAGCTG CGCACCTTCT 
     2221 TCCGCAAAGG GGGCGGTCCT TGCCTCTCCA ACACCTCGGC GCCGGGGCTC CTGGTGCTGC 
     2281 CCAGCCGCTG CGGAAACGGC TTCTTGGAAG CAGGAGAAGA GTGCGACTGC GGTTCTGGCC 
     2341 AGAAGTGCCC AGACCCCTGC TGCTTTGCCC ACAATTGCTC CCTGCGTGCG GGGGCTCAAT 
     2401 GTGCCCACGG TGATTGCTGT GCGAGGTGCC TGTTAAAGTC CGCGGGCACG CCTTGTCGTC 
     2461 CTGCTGCGAC TGACTGCGAT CTCCCCGAGT TCTGCACCGG CACCTCCCCG TATTGCCCCG 
     2521 CAGATGTTTA CCTACTGGAT GGCTCACCCT GCGCTGAGGG TCGCGGCTAT TGCCTAGACG 
     2581 GCTGGTGTCC CACGCTGGAG CAGCAGTGCC AGCAGCTATG GGGGCCTGGG TCCAAGCCGG 
     2641 CCCCAGAGCC ATGTTTCCAG CAGATGAACT CCATGGGGAA TTCGCAAGGG AACTGTGGCC 
     2701 AGGACCACAA GGGTAGCTTC CTGCCTTGTG CTCAGAGGGA CGCTCTGTGT GGGAAACTGC 
     2761 TGTGCCAGGG AGGGGAGCCG AACCCACTAG TGCCGCACAT AGTGACTATG GACTCCACAA 
     2821 TTCTCCTAGA GGGCCGCGAA GTGGTTTGCC GAGGGGCCTT TGTGCTCCCA GATAGTCACC 
     2881 TGGACCAGCT TGACTTGGGT CTGGTAGAGC CAGGCACCGG CTGTGGACCT AGAATGGTTT 
     2941 GCAATAGCAA TCGTAACTGT CACTGTGCTG CTGGCTGGGC TCCACCCTTC TGTGACAAGC 
     3001 CTGGCTTGGG TGGTAGCGTG GATAGTGGCC CTGCACAGTC TGCAAACCGA GATGCCTTCC 
     3061 CCTTGGCCAT GCTCCTCAGC TTCCTGCTGC CTCTGCTCCC TGGGGCTGGC CTAGCCTGGT 
     3121 GCTACTACCA GCTCCCAACA TTCTGTCATC GAAGGGGACT GTGCTGCAGG AGGGACCCCC 
     3181 TATGGAATAG AGACATACCC CTGGGCAGTG TGCATCCGGT GGAGTTTGGC TCCATCATCA 
     3241 CTGGAGAGCC CTCGCCCCCT CCCCCATGGA CCTCTTGCCA ACAGCGTTCG CACCCTCCAT 
     3301 CTCTTGACTT GCTCTCAGAC CCTGCGAACT CTGAGCTTAC CACGCGTACG CGGCCGCTCG 
     3361 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3421 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3481 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3541 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3601 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3661 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3721 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3781 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3841 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3901 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3961 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4021 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4081 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4141 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4201 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4261 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4321 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4381 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4441 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4501 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4561 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4621 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4681 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4741 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4801 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4861 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4921 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4981 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5041 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5101 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5161 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5221 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5281 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5341 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5401 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5461 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5521 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5581 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5641 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5701 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5761 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5821 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5881 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5941 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6001 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6061 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6121 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6181 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6241 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6301 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6361 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6421 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6481 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6541 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6601 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6661 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6721 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6781 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6841 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6901 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6961 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7021 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7081 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7141 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//