LOCUS       MR218633L3                8648 bp    DNA     circular  SYN 
DEFINITION  Cerk (Myc-DDK-tagged) - Mouse ceramide kinase (Cerk)
ACCESSION   NM_145475
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6939..7119 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7847..8506 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7167..7786 
                     /label="ColE1"  
     misc_feature    4979..5002 
                     /label="DDK"  
     misc_feature    5814..5834 
                     /label="LR50"  
     misc_feature    4931..5002 
                     /label="MYC"  
     misc_feature    5012..5077 
                     /label="P2A"  
     misc_feature    5078..5677 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    5823..6414 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4913..4919 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4913 
                     /label="Cerk"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGGGCAATG GGGGCGGCGG AGCCGCTGCA 
     3361 CTCCGTGCTG TGGGTGAAAC GGCGACGCTG TGCCGTCAGC CTGGAGCCCG CGCGGGCTCT 
     3421 GCTACGCTGG TGGCGGAGCC CGGAGCCCGG GCCCTCCGCG CCGGGTGCCG ATGCCCGCTC 
     3481 GGTACTGGTG TCGGAGATCA TCGCTGTTGA GGAAAAAGAC GACTGCGAGA AACACGCCTC 
     3541 CAGTGGCCGA TGGCATAAAA TGGAAAATCC GTTCGCATTC ACAGTCCACC GTGTGAAGCG 
     3601 AGTACGACAT CACCGCTGGA AGTGGGCACG GGTGACCTTC TGGAGCGCCG ACGAGCAGCT 
     3661 GTGTCACCTG TGGCTGCAGA CCCTCCGTGG GCTGCTGGAG AGCCTGACTT CAAGACCGAA 
     3721 GCACTTGCTG GTATTCATCA ACCCTTTCGG AGGGAAAGGT CAGGGCAAGC GCATCTATGA 
     3781 AAAAACAGTG GCGCCTCTGT TTACCTTGGC TTCCATCACT ACGGAGATCA TCATTACAGA 
     3841 GCATGCCAAC CAAGCCAAGG AGACTTTATA CGAGATCAAC ACAGACAGCT ATGATGGCAT 
     3901 CGTGTGCGTA GGTGGGGACG GCATGTTCAG CGAGGTGCTG CATGGGGTGA TTGGGAGGAC 
     3961 GCAGCAGAGC GCTGGTATCG ACCCCAATCA CCCCCGAGCC GTGCTGGTGC CCAGTACCCT 
     4021 CAGGATCGGC ATCATACCCG CAGGGTCCAC AGATTGTGTG TGTTACTCAA CAGTGGGCAC 
     4081 AAACGACGCA GAGACATCGG CTTTGCACAT CATTATTGGG GACTCACTGG CAATAGACGT 
     4141 GTCCTCTGTG CACTACCATA ACACGCTGCT GCGGTACTCG GTTTCTCTGC TGGGCTACGG 
     4201 TTTCTACGGG GACTTAATCA AGGACAGTGA AAAGAAACGG TGGATGGGCC TCGTCCGGTA 
     4261 TGATTTCTCA GGGTTGAAGA CCTTTCTCTC TCATCAGTAC TATGAAGGGA CACTGTCCTT 
     4321 CCTCCCAGCA CAGCACACGG TGGGATCTCC ACGGGACAAT AAACCCTGCC GGGCTGGGTG 
     4381 CTTCGTGTGC AGGCAGAGCA AGCAACAGCT GGAAGAAGAA GAGAAGAAAG CCCTGTATGG 
     4441 CCTGGAGAAC GCCGAGGAAA TGGAAGAGTG GCAAGTGACA TGTGGGAAGT TCCTGGCCAT 
     4501 CAATGCCACC AACATGTCCT GTGCTTGTCC TCGGAGCCCT GGGGGCCTGT CCCCATTTGC 
     4561 CCATCTGGGA GATGGGTCTT CTGACCTCAT CCTTATCCGG AAGTGCTCCA GGTTCAACTT 
     4621 CCTGAGATTC CTCATCCGGC ACACGAACCA GGAGGACCAG TTCGACTTCA CTTTCGTTGA 
     4681 AGTTTATCGA GTCAAGAAAT TCCACTTCAC GTCGAAGCAC GTGGAAGACG AGGACAATGA 
     4741 CTCGAAGGAA CAAGAGAAGC AGAAGTTTGG GAAGATCTGC AAGGACAGAC CCTCTTGCAC 
     4801 TTGCTCAGCC TCCAGAAGCT CCTGGAACTG CGATGGCGAA GTCATGCACA GCCCGGCCAT 
     4861 TGAGGTCAGG GTCCACTGCC AGCTGGTGCG CCTCTTTGCT CGGGGAATCG AGGAAGAGTC 
     4921 AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     4981 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTGGGTA GGAAGCGGAG CTACTAACTT 
     5041 CAGCCTGCTG AAGCAGGCTG GAGACGTGGA GGAGAACCCT GGACCTATGA CCGAGTACAA 
     5101 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC 
     5161 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGATCGCC ACATCGAGCG 
     5221 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG 
     5281 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG 
     5341 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC 
     5401 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT 
     5461 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT 
     5521 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC 
     5581 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC 
     5641 CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGATGTA CACCCAAACG 
     5701 GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA 
     5761 CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA 
     5821 AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 
     5881 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 
     5941 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 
     6001 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 
     6061 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 
     6121 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 
     6181 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC 
     6241 CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 
     6301 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 
     6361 TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC 
     6421 ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT 
     6481 ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT 
     6541 TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT 
     6601 AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA 
     6661 GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC 
     6721 ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA 
     6781 GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT 
     6841 GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC 
     6901 CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG 
     6961 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT 
     7021 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT 
     7081 AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA 
     7141 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 
     7201 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 
     7261 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 
     7321 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 
     7381 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 
     7441 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 
     7501 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 
     7561 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 
     7621 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 
     7681 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 
     7741 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 
     7801 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG 
     7861 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA 
     7921 AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT 
     7981 GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT 
     8041 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA 
     8101 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 
     8161 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG 
     8221 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT 
     8281 CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA 
     8341 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA 
     8401 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT 
     8461 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT 
     8521 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8581 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8641 CACCTGAC
//